2,348 research outputs found
Using the technology of the confessional as an analytical resource: four analytical stances towards research interviews in discourse analysis
Among the various approaches that have developed from FOUCAULT's work is an Anglophone
discourse analysis that has attempted to combine Foucaultian insights with the techniques of
Conversation Analysis. An important current methodological issue in this discourse analytical approach
is its theoretical preference for "naturally occurring" rather than research interview data. A Foucaultian
perspective on the interview as a research instrument, questions the idea of "naturally-occurring
discourse". The "technology of the confessional" operates, not only within research interviews, but
permeates other interactions as well. Drawing on FOUCAULT does not dismiss the problems of the
interview as research instrument rather it shows they cannot be escaped by simply switching to more
"natural" interactions. Combining these insights with recent developments within discourse analysis can
provide analytical resources for, rather than barriers to, the discourse analysis of research interviews. To
aid such an approach, we develop a four-way categorisation of analytical stances towards the research
interview in discourse analysis. A demonstration of how a research interview might be subjected to a
discourse analysis using elements of this approach is then provided
Computational Geometry Column 42
A compendium of thirty previously published open problems in computational
geometry is presented.Comment: 7 pages; 72 reference
Genomics clarifies taxonomic boundaries in a difficult species complex.
Efforts to taxonomically delineate species are often confounded with conflicting information and subjective interpretation. Advances in genomic methods have resulted in a new approach to taxonomic identification that stands to greatly reduce much of this conflict. This approach is ideal for species complexes, where divergence times are recent (evolutionarily) and lineages less well defined. The California Roach/Hitch fish species complex is an excellent example, experiencing a convoluted geologic history, diverse habitats, conflicting species designations and potential admixture between species. Here we use this fish complex to illustrate how genomics can be used to better clarify and assign taxonomic categories. We performed restriction-site associated DNA (RAD) sequencing on 255 Roach and Hitch samples collected throughout California to discover and genotype thousands of single nucleotide polymorphism (SNPs). Data were then used in hierarchical principal component, admixture, and FST analyses to provide results that consistently resolved a number of ambiguities and provided novel insights across a range of taxonomic levels. At the highest level, our results show that the CA Roach/Hitch complex should be considered five species split into two genera (4 + 1) as opposed to two species from distinct genera (1 +1). Subsequent levels revealed multiple subspecies and distinct population segments within identified species. At the lowest level, our results indicate Roach from a large coastal river are not native but instead introduced from a nearby river. Overall, this study provides a clear demonstration of the power of genomic methods for informing taxonomy and serves as a model for future studies wishing to decipher difficult species questions. By allowing for systematic identification across multiple scales, taxonomic structure can then be tied to historical and contemporary ecological, geographic or anthropogenic factors
Thermal Infrared Observations of Asteroid (99942) Apophis with Herschel
The near-Earth asteroid (99942) Apophis is a potentially hazardous asteroid.
We obtained far-infrared observations of this asteroid with the Herschel Space
Observatory's PACS instrument at 70, 100, and 160 micron. These were taken at
two epochs in January and March 2013 during a close Earth encounter. These
first thermal measurements of Apophis were taken at similar phase angles before
and after opposition. We performed a detailed thermophysical model analysis by
using the spin and shape model recently derived from applying a 2-period
Fourier series method to a large sample of well-calibrated photometric
observations. We find that the tumbling asteroid Apophis has an elongated shape
with a mean diameter of 375 m (of an equal volume sphere) and a
geometric V-band albedo of 0.30. We find a thermal inertia in
the range 250-800 JmsK (best solution at 600
JmsK), which can be explained by a mixture of low
conductivity fine regolith with larger rocks and boulders of high thermal
inertia on the surface. The thermal inertia, and other similarities with
(25143) Itokawa indicate that Apophis might also have a rubble-pile structure.
If we combine the new size value with the assumption of an Itokawa-like density
and porosity we estimate a mass between 4.4 and 6.2 10 kg which is more
than 2-3 times larger than previous estimates. We expect that the newly derived
properties will influence impact scenario studies and influence the long-term
orbit predictions of Apophis.Comment: Accepted for publication in Astronomy & Astrophysics, 21 pages, 8
figures, 2 table
Physical Properties of OSIRIS-REx Target Asteroid (101955) 1999 RQ36 derived from Herschel, ESO-VISIR and Spitzer observations
In September 2011, the Herschel Space Observatory performed an observation
campaign with the PACS photometer observing the asteroid (101955) 1999 RQ36 in
the far infrared. The Herschel observations were analysed, together with ESO
VLT-VISIR and Spitzer-IRS data, by means of a thermophysical model in order to
derive the physical properties of 1999 RQ36. We find the asteroid has an
effective diameter in the range 480 to 511 m, a slightly elongated shape with a
semi-major axis ratio of a/b=1.04, a geometric albedo of 0.045 +0.015/-0.012,
and a retrograde rotation with a spin vector between -70 and -90 deg ecliptic
latitude. The thermal emission at wavelengths below 12 micron -originating in
the hot sub-solar region- shows that there may be large variations in roughness
on the surface along the equatorial zone of 1999 RQ36, but further measurements
are required for final proof. We determine that the asteroid has a
disk-averaged thermal inertia of Gamma = 650 Jm-2s-0.5K-1 with a 3-sigma
confidence range of 350 to 950 Jm-2s-0.5K-1, equivalent to what is observed for
25143 Itokawa and suggestive that 1999 RQ36 has a similar surface texture and
may also be a rubble-pile in nature. The low albedo indicates that 1999 RQ36
very likely contains primitive volatile-rich material, consistent with its
spectral type, and that it is an ideal target for the OSIRIS-REx sample return
mission.Comment: Accepted for publication in Astronomy & Astrophysics, 9 pages, 7
figure
Are we all on course? A curriculum mapping comparison of three australian university open-access enabling programs.
© 2019, Adult Learning Australia Inc. All rights reserved.
The curricula in higher education not only provides guidance and direction for students, but aligns with industry standards to safeguard quality offerings in specific programs. While there has been increasing focus on the importance of the curriculum particularly for first year university students, very little is known about the curriculum and design principles that exist in open-access enabling programs in Australia. In the following paper, a comprehensive examination of the curriculum of three large open-access enabling programs is presented. The research team explored the curriculum design via a rigorous mapping exercise to establish potential principles to guide enabling curriculum design. In developing the curriculum-mapping tool for this research project, it became apparent that limited attention has been given to enabling curriculum design in the academic literature. Given increasing attention towards Australian enabling education, the findings of this study emphasise the importance of clarity between the intended and the enacted curriculum, such as in unit learning outcomes and program attributes
SYSTEMS-2: a randomised phase II study of radiotherapy dose escalation for pain control in malignant pleural mesothelioma
SYSTEMS-2 is a randomised study of radiotherapy dose escalation for pain control in 112 patients with malignant pleural mesothelioma (MPM). Standard palliative (20Gy/5#) or dose escalated treatment (36Gy/6#) will be delivered using advanced radiotherapy techniques and pain responses will be compared at week 5. Data will guide optimal palliative radiotherapy in MPM
P05-14 LB. Analysis of virus swarms to identify vaccine immunogens with improved exposure of epitopes recognized by broadly neutralizing antibodies
Recommended from our members
A multi-well plate model of reactive gliosis for high throughput screening of potential CNS therapies
Reactive astrogliosis is an important feature of CNS damage and disease which involves changes in astrocyte phenotype and morphology. In particular, CNS trauma can lead to the formation of a glial scar, a three dimensional (3D) mesh of astrocyte processes that can form a physical and chemical barrier to neuronal regeneration, which is a potential target for CNS drug and cell therapy. However, reactive gliosis is difficult to isolate and monitor in typical animal models of CNS damage. In monolayer culture systems astrocytes adopt a highly reactive phenotype, limiting the range of available models suitable for research in this area.
Our previous 3D cell culture systems allow astrocytes to be maintained with a relatively unreactive phenotype until stimulated, whereupon a classical reactive astrocyte response can be monitored. The aim of the current work is to adapt this approach in order to develop a multi-well plate system to provide a reliable, consistent model of reactive gliosis for high throughput screening and research. Once baseline viability and phenotype of primary rat astrocytes were investigated in these models, reactivity was triggered using treatments such as TGFβ1, as seen in Figure 1, hypoxia and low glucose. Outputs included confocal microscopy and 3D image analysis, Western blotting and RT-PCR to quantify markers such as GFAP and CSPG in test and control gels. Using GFP-labelled astrocytes permitted monitoring of cytoplasmic volume and shape, giving an additional measure of astrocyte hypertrophic response within stimulated conditions. The robust protocol that we have developed can form a basis to investigate astrocyte biology in a highly controlled environment, and to model phenotypic features of astrocytes in both damaged and undamaged CNS. A reproducible multi-well plate system will provide an experimental platform which allows potential CNS therapies to be screened at high-throughput, and the effects of potential modulators of astrocyte reactivity to be investigated simply and systematically
Recommended from our members
Defining glial cell self-alignment parameters for 3D CNS tissue models
- …