27 research outputs found
The welfare of animals transported from Ireland to Spain AND The Physiological haematological and immunological responses of 9-month old bulls (250kg) to transport at two stocking densities (0.85m2 and 1.27m2 /250kg animal) on a 12-hour journey by road.
End of Project ReportFifty-two weanling continental x beef heifers (mean liveweight 269kg) were transported from
Ireland to France on a roll-on roll-off ferry (RO-RO), and onwards by road for 3-hours to a French
lairage, rested for 24 hours at a staging post and taken by road on an 18-hour journey through
France to a feedlot in Spain. Animals transported to France lost 7.6 % of their bodyweight, and
gained 3.3 % of their bodyweight by time of arrival in Spain and recovered to pre-transport
liveweight values by day 6. Although there was some evidence that transport affected physiological
and immunological variables, there was no evidence to suggest that it adversely affected the health
or the performance of the animals post transport.
Creatine kinase activities were increased but values were still within normal acceptable ranges.
Increases in non-esterified fatty acids, beta-hydroxybutyrate and urea concentrations suggested that
the animals' normal pattern of feeding was disrupted during transport. Increases in albumin, total
plasma protein and osmolality would indicate slight dehydration during transit. However, albumin
concentrations returned to control levels by day 38 of the study. While haematocrit values were
decreased, they are within the range of normal referenced data (24 - 48%). Similarly, changes in the
RBC numbers and haemoglobin were within the normal blood referenced ranges ((RBC; 5.0 â 10.0
x106 /ml) and (haemoglobin 8-14 g%)(Schalm, 1961)). The only time at which white blood counts
increased above the upper limit of 12, was 12 hours after arrival at the French lairage. The
aspartate transaminase concentrations for the transported animals at arrival in France and Spain
were not significantly different from their pre-transport concentrations but were increased at day 11
when compared with baseline levels.
Concanavalin-A induced interferon-g levels were lower on arrival in the Spanish feedlot and on Day
11 of the study, when compared with pre-transport baseline levels. Compared with pre-transport
levels, keyhole limpet haemocyanin-induced interferon-g levels for the transported animals were
significantly decreased on the day of arrival in France, with no significant difference on the day of
arrival in Spain or on day 11 of the study. Interferon-g is produced by activated T lymphocytes and
natural killer cells in response to antigen. The percentage (%) of lymphocytes decreased and the %
neutrophils increased post-transport indicating a shift in the population of these blood cells relative
to pre-transport baseline values. There was no significant change in plasma cortisol concentrations
in transported animals at arrival in France and in Spain. On Day 11, the plasma cortisol
concentrations of transported animals were significantly higher than control animals.
There were significantly higher glucose concentrations on arrival in France, and in samples taken at
12 and 24 hours post-arrival in France, on arrival in Spain, and on days 7 and 11 compared with
control levels. Transported animals had significantly higher glucose levels at sample 2 on the day of
arrival in France compared with their pre-transport values.
Transported animals had significantly higher fibrinogen levels at arrival in France compared with
their pre-transport baseline concentrations. Inflammation resulting from stress can cause the release
of acute phase proteins such as haptoglobin and fibrinogen, and acute phase proteins in cattle have
been associated with immunosuppression, however, much higher levels have been reported in
inflammatory conditions. Transported animals had significantly higher non-esterified fatty acid
(NEFA) levels on arrival in France and Spain and on day 11 compared with their pre-transport
baseline concentrations. Control animals had significantly higher levels on day 5 compared with
their pre-transport baseline NEFA concentrations. However, all levels were within the normal
acceptable ranges.
The study concluded that transport had no adverse effect on animal welfare based on the
physiological, immunological and haematological measurements made
The Welfare of Animals Transported From Ireland to Italy.
End of Project ReportThe overall objective of the present study was to investigate
the physiological, haematological and immunological responses of
weanling bulls transported to Italy under present EU legislation and
to evaluate the implications in terms of animal welfare
Effects of Pre-Journey Fasting on the Physiological Responses of Young Cattle to 8-hour Road Transport.
End of Project ReportThe present study evaluated the effects of fasting animals for 8
hours prior to an 8-hour road journey and their ability to cope with
the stress of transport.There was no significant difference in rectal
body temperature, pre and post transport and there were no
significant differences in liveweight among treatments on days 0
(pre-transport), 1, 4 and 10 (post-transport). Bulls (230kg) undergoing
an 8-h transportation at stocking densities of 0.82 m2 /animal
showed physiological and haematological responses that were
within normal referenced ranges. Animals that were fasted for
8-hours and then transported lost 9.4% bodyweight following the
8-hour journey, while non-fasted and transported animals (NF+T)
lost 7.2%. The control animals remaining at grass and non-fasted
(NF+G) gained 2%. The animals that were fasted continuously and
not transported (F+F) and the non-fasted control animals that were
fasted for 8 hours (NF+F) lost 6.1% and 6.2% respectively.
There was no significant change in globulin, glucose, urea, haemoglobin,
beta-hydroxy butyrate, fibrinogen concentrations, haematocrit
and monocyte percentages, monocyte and red blood cell numbers,
platelet numbers among treatments prior to or after transport.
The % lymphocytes were reduced in the fasted and non-fasted
transported animals and post-transport and there was no significant
change in lymphocyte numbers. The % of neutrophils and the
number of neutrophils were significantly increased in the fasted and
non-fasted transported animals. Baseline protein concentrations
were significantly lower in the non-fasted and transported and nonfasted
then fasted treatments initially. Following transport, protein
concentrations were significantly higher in the fasted and transported
treatment compared with the non-fasted animals at grass.
White blood cell (WBC) numbers were not significantly different
prior to transport. Following transport, the WBC numbers were
significantly higher in the fasted and transported treatment compared
with the non-fasted at grass, fasted and then fasted, and the
non-fasted and fasted treatments. Albumin concentrations were
significantly higher following transport in the F+T treatment compared
with the NF+G, F+F, and NF+F treatments and the NF+T
treatment had significantly lower albumin levels than the F+T and
NF+F treatments. Haptoglobin concentrations were not significantly
different prior to transport. Following transport, haptoglobin concentrations
were significantly higher in the F+T compared with the
NF+G treatment. Lactate concentrations were significantly higher in
the F+T and NF+T compared with the NF+G, F+F, and NF+F treatments
following transport.
In conclusion, from the physiological and haematological
measurements, an 8 hour journey time, even without access to feed
for 8 hours prior to transport did not impact negatively on animal
welfare
Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries
Background
Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres.
Methods
This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and lowâmiddle-income countries.
Results
In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of âsingle-useâ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for lowâmiddle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia.
Conclusion
This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both highâ and lowâmiddleâincome countries
Organocatalytic Michael additionâlactonisation of carboxylic acids using α,ÎČ-unsaturated trichloromethyl ketones as α,ÎČ-unsaturated ester equivalents
The authors thank the Royal Society for a University Research Fellowship (ADS), The Carnegie Trust for the Universities of Scotland (LCM), Syngenta/EPSRC (Case award to DGS), GSK/EPSRC (CASE award to SRS), the EU (IEF for CS), and the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013) ERC Grant Agreement no. 279850 (JET) for funding.Isothiourea HBTM-2.1 catalyses the Michael addition-lactonisation of 2-aryl and 2-alkenylacetic acids and α,ÎČ-unsaturated trichloromethyl ketones. Ring-opening of the resulting dihydropyranones and subsequent alcoholysis of the CCl ketone with an excess of methanol gives a range of diesters in high diastereo- and enantioselectivity (up to 95:5 dr and >99% ee). Sequential addition of two different nucleophiles to a dihydropyranone gives the corresponding differentially substituted diacid derivative.PostprintPeer reviewe
Organocatalytic Michael additionâlactonisation of carboxylic acids using α,ÎČ-unsaturated trichloromethyl ketones as α,ÎČ-unsaturated ester equivalents
Isothiourea HBTM-2.1 catalyses the Michael addition-lactonisation of 2-aryl and 2-alkenylacetic acids and α,ÎČ-unsaturated trichloromethyl ketones. Ring-opening of the resulting dihydropyranones and subsequent alcoholysis of the CCl ketone with an excess of methanol gives a range of diesters in high diastereo- and enantioselectivity (up to 95:5 dr and >99% ee). Sequential addition of two different nucleophiles to a dihydropyranone gives the corresponding differentially substituted diacid derivative
On a uniformly accurate finite difference approximation of a singularly perturbed reaction-diffusion problem using grid equidistribution
We examine the convergence properties of a finite difference approximation of a singularly perturbed reaction-diffusion boundary value problem using a nonuniform grid. The grid is based on the equidistribution of a positive monitor function that is a linear combination of a constant floor and a power of the second derivative of the solution. Analysis shows how the monitor function can be chosen to ensure that the accuracy of the numerical approximation is insensitive to the size of the singular perturbation parameter. The use of equidistribution principles appears in many practical grid adaption schemes and our analysis provides insight into the convergence behaviour on such grids. Numerical results are given that confirm the uniform convergence rates