The Role of Occupational Therapy in Rural Healthcare: A Case Study on Farmers with Disabilities

The impact of disability on farmers' personal affairs and work capacity has consequences not only on farmers, but also on their families, farm operations, and communities. Living in a rural area can play a role in recovery after disease or injury in that beneficial therapy services may not be available due to barriers such as meeting the needs of a widespread population, lack of physician referral, and travel time. To gain the perspective of the impact of disability on farmers' lives, face-to-face interviews were conducted with two farmers in rural Nebraska. Through these discussions five similar themes emerged which include: adaptations, safety, family assistance, therapy services, and specialty services

A national open-access research registry to improve recruitment to clinical studies.

IntroductionBarriers to recruitment for dementia studies are well documented. As part of the UK government's Dementia 2020 strategy, a nationally consistent system to increase public engagement and participation in research was launched in February 2015.MethodsWe describe the development of the "Join Dementia Research" registry, including evolution of policy, involvement of people with dementia in co-production, data requirements, governance, technology, and the impact on study recruitment and what factors may have contributed to the services success.ResultsThe UK-wide online, telephone, and postal service has registered 47,071 volunteers, with 33,139 people (67.9% of all volunteers) taking part in 378 studies, with 49,954 total study enrolments. This has taken place across 295 research sites, involved 1522 researchers, and resulted in 134 peer-reviewed publications.DiscussionPublic registries of individuals interested in research, with user-provided data enabling basic phenotyping, are effective at increasing public engagement with research and removing barriers to study recruitment. Deeper pheno/genotyping could be undertaken to improve matching, but how and when that information is collected will be a key factor

Art expertise in construing meaning of representational and abstract artworks

Aesthetic appraisal of artwork can present the observer with visual problems to solve in the process of grasping its meaning and ‘visual rightness’ (i.e. “good” structure; Locher, 2003), with an elaboration on perceptual, semantic and affective dimensions (e.g. Marković, 2011). Thus observer's expertise is a factor in aesthetic appraisal. To examine the influence of art training on the aesthetic response, and to clarify the nature of the Representational/Abstract distinction, 30 Experts and 33 Non-experts (Art and Psychology students, respectively) were asked to rate 24 paintings on six affective and affective-evaluative semantic differential scales. Stimuli were images of paintings from the period 1900–1935, 12 broadly Representational and 12 broadly Abstract. Relative to Non-experts, Experts rated Abstract artworks as more Interesting, Beautiful, Informative and Sophisticated, distinguishing them less markedly from Representational artworks. Aggregate Expert and Non-expert ratings, processed by factor analysis, resulted in a two-factor solution. The first factor, contrasting Abstract and Representational artworks, appeared more salient for Non-experts. The second factor, Cool–Warm, separating vibrantly-colored paintings from those with a blue-dominated/dull palette, was more salient for Experts. While Non-experts exaggerated differences between Abstract and Representational paintings, Experts appraised these two types of art similarly, attending more to artwork collative properties. We conclude that appreciation of art by Experts involves ‘cognitive mastery’ (Leder, Belke, Oeberst, & Augustin, 2004), i.e. more complex, cues-based visual schemata which equip them with more sophisticated strategies for analysing collative properties and semantics of an artwork while parsing ‘visual rightness’ to unfold its visual meaning

Mitochondrial Alterations in PINK1 Deficient Cells Are Influenced by Calcineurin-Dependent Dephosphorylation of Dynamin-Related Protein 1

PTEN-induced novel kinase 1 (PINK1) mutations are associated with autosomal recessive parkinsonism. Previous studies have shown that PINK1 influences both mitochondrial function and morphology although it is not clearly established which of these are primary events and which are secondary. Here, we describe a novel mechanism linking mitochondrial dysfunction and alterations in mitochondrial morphology related to PINK1. Cell lines were generated by stably transducing human dopaminergic M17 cells with lentiviral constructs that increased or knocked down PINK1. As in previous studies, PINK1 deficient cells have lower mitochondrial membrane potential and are more sensitive to the toxic effects of mitochondrial complex I inhibitors. We also show that wild-type PINK1, but not recessive mutant or kinase dead versions, protects against rotenone-induced mitochondrial fragmentation whereas PINK1 deficient cells show lower mitochondrial connectivity. Expression of dynamin-related protein 1 (Drp1) exaggerates PINK1 deficiency phenotypes and Drp1 RNAi rescues them. We also show that Drp1 is dephosphorylated in PINK1 deficient cells due to activation of the calcium-dependent phosphatase calcineurin. Accordingly, the calcineurin inhibitor FK506 blocks both Drp1 dephosphorylation and loss of mitochondrial integrity in PINK1 deficient cells but does not fully rescue mitochondrial membrane potential. We propose that alterations in mitochondrial connectivity in this system are secondary to functional effects on mitochondrial membrane potential

Interactions between the juvenile Batten disease gene, CLN3, and the Notch and JNK signalling pathways

Mutations in the gene CLN3 are responsible for the neurodegenerative disorder juvenile neuronal ceroid lipofuscinosis or Batten disease. CLN3 encodes a multi-spanning and hydrophobic transmembrane protein whose function is unclear. As a consequence, the cell biology that underlies the pathology of the disease is not well understood. We have developed a genetic gain-of-function system in Drosophila to identify functional pathways and interactions for CLN3. We have identified previously unknown interactions between CLN3 and the Notch and Jun N-terminal kinase signalling pathways and have uncovered a potential role for the RNA splicing and localization machinery in regulating CLN3 function