Radionuclude Levels in Caribou of Northern Alaska in 1995-96

Caribou (Rangifer tarandus) were sampled (1995-96) from a mortality event near the Project Chariot site (the location of a radiotracer experiment in northwestern Alaska during the 1960s) and reference sites. Radionuclide levels in muscle and bone and the cause(s) of the mortality were determined because of local residents' concerns. Bone gross alpha mean activity (n=65) was 130.0 Bq/kg, and varied significantly (p<0.01) from 73.3 to 168.0 Bq/kg among locations. Bone gross beta mean activity was 510.4, and muscle gross beta mean activity was 9.78 Bq/kg. Bone strontium-90 mean activity (n=58) was 137.8 Bq/kg. Muscle potassium-40 mean activity (n=65) was 83.0 Bq/kg, and varied significantly from 76.0 to 104.4 Bq/kg among locations. Muscle cesium-137 mean activity (n=65) was 6.67 Bq/kg, ranged significantly from 0.74 to 15.6 Bq/kg by location, and increased with increasing body condition score. Bone potassium-40 mean activity ranged from 18.9 to 47.4 Bq/kg, and muscle strontium-90 ranged from 8.89 to 20.0 Bq/kg. Radionuclide concentrations were at expected levels. In some cases, they were low compared to those in Canadian caribou studies.En 1995 et 1996, on a prélevé des échantillons de caribou (Rangifer tarandus) morts accidentellement près de l'emplacement du projet Chariot (N.-O. de l'Alaska), sur les lieux d'une expérience menée avec des radiotraceurs dans les années 1960 et sur des lieux de référence. On a déterminé les niveaux de radionucléides dans les muscles et les os ainsi que la ou les causes de mortalité afin de répondre aux questions que se posaient les résidents de la région. La radioactivité moyenne brute alpha dans les tissus osseux (n = 65) était de 130,0 Bq/kg, et variait largement (p < 0,01), de 73,3 à 168,0 Bq/kg selon les emplacements. La radioactivité moyenne brute bêta dans les tissus osseux était de 510,4 et celle dans les tissus musculaires était de 9,78 Bq/kg. La radioactivité moyenne du strontium 90 (n = 58) était de 137,8 Bq/kg. La radioactivité moyenne du potassium 40 (n = 65) dans les tissus musculaires était de 183,0 Bq/kg, et elle variait largement, de 76,0 à 104,4 Bq/kg selon les emplacements. La radioactivité moyenne du césium 137 (n = 65) dans les tissus musculaires était de 6,67 Bq/kg et variait largement, de 0,74 à 15,6 Bq/kg selon l'emplacement; son augmentation suivait celle de la cote de l'état du corps. La radioactivité moyenne du potassium 40 dans les tissus osseux allait de 18,9 à 47,4 Bq/kg, et celle du strontium 90 dans les tissus musculaires allait de 8,89 à 20,0 Bq/kg. Les niveaux de concentration en radionucléides étaient ceux auxquels on s'attendait. Dans certains cas, ils étaient bas, par rapport à ceux d'études canadiennes sur le caribou

Human Health Implications of Omega-3 and Omega-6 Fatty Acids in Blubber of the Bowhead Whale (Balaena mysticetus)

Concerns exist regarding the health and nutrition of subsistence-based communities in Alaska. An apparent increase in diabetes, heart disease, obesity, and other disease conditions among Alaska Natives has accompanied their change from a traditional diet to a more “Western” diet. In northern Alaska, the meat, maktak (epidermis and blubber), and other products of bowhead whales provide important components of Native diets. This study assessed the fatty acid constituents of bowhead whale blubber to evaluate their possible health benefits. Working with hunters in Barrow, Alaska, we acquired samples for chemical analysis from five blubber depths at each of six body locations. We used gas chromatography-mass spectrometry of fatty-acid picolinyl esters to confirm the fatty-acid composition of samples. Analyses indicated that bowhead blubber contains relatively high levels of omega-3 fatty acids and that, on average, blubber samples from sites at the umbilical girth contain more omega-3 fatty acids than do samples from a girth 1 m caudal to the blowhole (roughly at the axillary girth). Omega-6 fatty acids were rare or undetectable in all samples. Omega-3 fatty acids have been suggested or shown to be important in the treatment or prevention of many diseases, including elevated blood pressure and cholesterol, heart disease, stroke, diabetes, arthritis, depression, and some cancers. Beyond the cultural benefits associated with subsistence hunting of bowhead whales, consumption of bowhead whale blubber provides some important health and nutritional benefits.Il existe des inquiétudes quant à la santé et à l’alimentation des collectivités basées sur la subsistance en Alaska. L’augmentation apparente du diabète, des maladies cardiaques, des cas d’obésité et d’autres maladies chez les Autochtones de l’Alaska va de pair avec leur passage d’un régime alimentaire traditionnel à un régime plus « occidental ». Dans le nord de l’Alaska, la viande, maktak (épiderme et petit lard), et d’autres produits de la baleine boréale représentent d’importantes composantes du régime alimentaire des Autochtones. Dans le cadre de cette étude, nous nous sommes penchés sur les composants en acides gras du petit lard de la baleine boréale et ce, afin de déterminer leurs bienfaits possibles sur la santé. De concert avec des chasseurs de Barrow, en Alaska, nous avons prélevé des échantillons de cinq épaisseurs de petit lard provenant de chacun de six endroits différents du corps afin d’en faire l’analyse chimique. Nous avons utilisé la chromatographie en phase gazeuse et la spectrométrie de masse d’esters picoliniques d’acides gras pour confirmer la composition en acides gras des échantillons. Les analyses laissaient supposer que le petit lard de la baleine boréale a une teneur relativement élevée en acides gras oméga-3 et, qu’en moyenne, les échantillons de petit lard provenant des endroits situés à la hauteur ombilicale renferment de plus grandes quantités d’acides gras oméga-3 que les échantillons provenant d’un endroit situé 1 m de la queue jusqu’à l’évent (environ à la hauteur axillaire). Dans tous les échantillons, les acides gras oméga-6 se faisaient rares, voire même indécelables. Certaines recherches portent à croire ou démontrent que les acides gras oméga-3 jouent un rôle important dans le traitement ou la prévention de nombreuses maladies, dont l’hypertension artérielle, le taux de cholestérol élevé, les maladies du coeur, les accidents cérébrovasculaires, le diabète, l’arthrite, la dépression et certains cancers. En plus des avantages culturels liés à la chasse de subsistance de la baleine boréale, la consommation du petit lard de la baleine boréale présente d’importants avantages du point de vue de la santé et de l’alimentation

Radionuclide Contaminant Burdens in Arctic Marine Mammals Harvested During Subsistence Hunting

We conducted gamma spectrometric analyses on more than 200 arctic marine mammal tissue samples. These samples were primarily provided by subsistence hunters from northern Alaska, with a smaller number of samples from the Resolute region in Canada. The majority of samples (>90%) had detectable levels of the anthropogenic radionuclide 137Cs, with a mean level observed in all samples of 0.67 Bq/kg dry weight ± 0.81 (SD). Converted to wet weight, the mean was 0.21 Bq/kg ± 0.19 SD. The median activity observed was 0.45 Bq/kg dry weight (0.18 Bq/kg wet weight) with a range from detection limits to 6.7 Bq/kg dry weight (1.1 Bq/kg wet weight). These findings confirm expectations that current anthropogenic gamma emitter burdens in marine mammals used in the North American Arctic as subsistence food resources are well below activities that would normally merit public health concern (~1000 Bq/kg wet weight). Some differences among species and tissues were observed. Beluga tissues had slightly higher mean burdens of 137Cs overall, and epidermis and muscle tissues in bowhead and beluga whales typically had higher burdens than other tissues analyzed. Low levels of the neutron activation product 108mAg (half-life 418 yr.), probably bioaccumulated from bomb fallout sources, were observed in 16 of 17 beluga livers analyzed, but were not found in any other tissues of beluga or in any other species sampled. A subset of 39 samples of various tissues was analyzed for the alpha and beta emitters 239,240Pu and 90Sr. Plutonium levels were near the threshold of detectability (~0.1 Bq/kg dry weight) in 6 of the 39 samples; all other samples had no detectable plutonium. A detectable level of 90Sr (10.3 ± 1.0 Bq/kg dry weight) was observed in only one of the 39 samples analyzed, a bowhead epidermis sample. Although the accumulation of 108mAg has not been previously reported in any marine mammal livers, all of our analytical measurements indicate that only very low levels of anthropogenic radioactivity are associated with marine mammals harvested and consumed in the North American Arctic.On a effectué des analyses gamma-spectrométriques sur plus de 200 échantillons de tissus prélevés sur des mammifères marins. La plupart de ces échantillons étaient fournis par des chasseurs de subsistance de l'Alaska septentrional, et un petit nombre venaient de la région de Resolute au Canada. La majorité des échantillons (> 90 p. cent) contenaient des niveaux détectables du radionucléide anthropique 137Cs, avec un niveau moyen observé dans tous les échantillons de 0,67 Bq/kg de poids sec ± 0,81 (écart-type). Convertie en poids frais, la moyenne était de 0,21 Bq/kg ± 0,19 d'écart-type. L'activité médiane observée était de 0,45 Bq/kg de poids sec (0,18 Bq/kg de poids frais) avec une fourchette allant des seuils de détection jusqu'à 6,7 Bq/kg de poids sec (1,1 Bq/kg de poids frais). Ces résultats confirment les réponses prévues, à savoir que les charges actuelles des émetteurs gamma anthropiques présentes chez les mammifères marins utilisés en Amérique du Nord comme ressource de subsistance sont bien inférieures aux niveaux qui voudraient normalement qu'on s'inquiète pour la santé publique (~1000 Bq/kg de poids frais). On a observé certaines différences dans les espèces et les tissus. Dans l'ensemble, les tissus prélevés sur le bélouga contenaient des charges moyennes de 137Cs légèrement plus élevées, et l'épiderme et les tissus musculaires de la baleine boréale et du bélouga avaient généralement des charges supérieures à celles trouvées dans les autres tissus analysés. Dans 16 des 17 foies de bélouga analysés, on a observé de faibles niveaux du produit d'activation neutronique 108mAg (demi-vie 418 années), dont la bioaccumulation est probablement due à des retombées de bombes atomiques, mais on n'en a observé aucune trace dans les autres tissus du bélouga ou de toute autre espèce échantillonnée. On a analysé un sous-ensemble de 39 échantillons provenant de tissus divers pour savoir s'ils contenaient des émetteurs alpha et bêta 239,240Pu et 90Sr. Dans 6 des 39 échantillons, les niveaux de plutonium étaient proches du seuil de détectabilité (~ 0,1 Bq/kg de poids sec), et on n'a pas trouvé de plutonium détectable dans aucun des autres échantillons. On a observé un niveau détectable de 90Sr (10,3 ± 1,0 Bq/kg de poids sec) dans un seul des 39 échantillons analysés, soit un échantillon d'épiderme de baleine boréale. Bien qu'on n'ait jamais rapporté auparavant une accumulation de 108mAg dans le foie d'un mammifère marin, toutes nos mesures analytiques révèlent que les mammifères marins faisant l'objet d'une activité d'exploitation et consommés dans l'Arctique nord-américain ne présentent que de très faibles niveaux de radioactivité anthropique

Is the early identification and referral of suspected head and neck cancers by community pharmacists feasible?:A qualitative interview study exploring the views of patients in North East England

INTRODUCTION Head and neck cancer (HNC) is the eighth most common cancer in the United Kingdom. Survival rates improve when the cancer is diagnosed at an early stage, highlighting a key need to identify at-risk patients. This study aimed to explore opportunistic HNC identification and referral by community pharmacists (CPs) using a symptom-based risk assessment calculator, from the perspective of patients with a diagnosis of HNC. METHODS Purposive sampling was used to recruit patients from the HNC pathway in three large teaching hospitals in Northern England. Qualitative methodology was used to collect data through an iterative series of semistructured telephone interviews. Framework analysis was utilised to identify key themes. RESULTS Four main themes were constructed through the analytic process: (1) HNC presentation and seeking help; (2) the role of the CP; (3) public perception of HNC and (4) the role of a symptom-based risk calculator. Participants agreed that CPs could play a role in the identification and referral of suspected HNCs, but there were concerns about access as patients frequently only encounter the medicine counter assistant when they visit the pharmacy. HNC symptoms are frequently attributed to common or minor conditions initially and therefore considered not urgent, leading to delays in seeking help. While there is public promotion for some cancers, there is little known about HNC. Early presentation of HNC can be quite variable, therefore raising awareness would help. The use of a symptom-based risk calculator was considered beneficial if it enabled earlier referral and diagnosis. Participants suggested that it would also be useful if the public were made aware of it and could self-assess their symptoms. CONCLUSION In principle, CPs could play a role in the identification and referral of HNC, but there was uncertainty as to how the intervention would work. Future research is needed to develop an intervention that would facilitate earlier identification and referral of HNC while not disrupting CP work and that would promote HNC and the risk calculator more widely. PATIENT OR PUBLIC CONTRIBUTION Patient and public involvement and engagement (PPIE) was integrated throughout the project. Initially, the proposal was discussed during a Cancer Head and Neck Group Experience (CHANGE) PPIE meeting. CHANGE was set up to support HNC research in 2018. The group is composed of seven members (four female, three male) with an age range of 50-71 years, who were diagnosed at Sunderland Royal Hospital. A patient representative from the University of Sunderland PPIE group and a trustee of the Northern HNC Charity were recruited as co-applicants. They attended project management group meetings and reviewed patient-facing documentation

Investigation of Heavy Metals in a Large Mortality Event in Caribou of Northern Alaska

We measured element concentrations (As, Cd, Cu, Pb, Zn, Fe) and body condition (gross and histologic endpoints) of animals from a caribou (Rangifer tarandus) mortality event that occurred in Alaska, in the area of Point Hope and Cape Thompson (including the Chariot site), in 1995. These were compared to results from hunter-killed caribou from reference sites (Barrow and Teshekpuk Lake, Alaska) and from the area of a mine (Red Dog Mine) to determine whether heavy metals had played a role in the mortality event or whether any elements were at concentrations of concern for human consumers. Starvation and malnutrition were major factors leading to death or severe weakness, as very little or no fat (very low body condition scores) and serous atrophy of fat (observed as watery contents of the marrow cavity, with no apparent fat, and histologically) were more prevalent in caribou associated with the mortality event than in hunter-killed animals from reference sites. Accumulation of hepatic (liver) hemosiderin in Kupffer cells (macrophages) was noted as an indicator of cachexia. Concentrations of lead in feces and liver, copper in the rumen contents, and arsenic in muscle were higher in caribou harvested near Red Dog Mine, as might be expected in that mineral-rich area, but were not at levels of concern for toxicoses. Kidney concentrations of cadmium, which increased significantly with increasing age, present a potential concern for human consumers, and this is an expected finding. We concluded that caribou had starved and that heavy metals had played no role in the mortality event. Further investigation of regional mineral differences is required to understand the sources and transport mechanisms that explain these findings and to properly address mining activity. Mortality events on the north slope of Alaska are common and likely involve starvation as described here, but in most cases they are not investigated, even though recent industrial activities have heightened concern among some local residents and wildlife managers.On a mesuré la concentration en éléments (As, Cd, Cu, Pb, Zn, Fe) et l'état corporel (points limites bruts et histologiques) de caribous (Rangifer tarandus) prélevés lors d'un épisode de mortalité qui s'est produit en 1995 en Alaska, dans la région de Point Hope et de Cape Thompson (y compris le site Chariot). On a comparé ces résultats à ceux de caribous tués par des chasseurs à des emplacements témoins (Barrow et Teshekpuk Lake, en Alaska) et à proximité d'une mine (Red Dog Mine) pour trouver si les métaux lourds avaient joué un rôle dans l'épisode de mortalité ou si la concentration d'un ou plusieurs éléments pouvait constituer un risque pour la consommation humaine. La famine et la malnutrition étaient des facteurs majeurs ayant causé la mort ou une extrême faiblesse, vu que la présence minime ou l'absence de graisse (très basses notes d'état corporel) et une atrophie séreuse de la graisse (observée sous forme de contenu aqueux de la cavité médullaire, sans graisse visible, et à la suite de l'examen histologique) étaient plus courantes chez le caribou associé à l'épisode de mortalité que chez les animaux des emplacements témoins tués par les chasseurs. On a noté dans le foie une accumulation d'hémosidérine hépatique des cellules de Kupffer (cellules macrophages) témoignant d'une cachexie. La concentration de plomb dans les matières fécales et le foie, de cuivre dans le rumen et d'arsenic dans le tissu musculaire était plus élevée chez le caribou provenant de Red Dog Mine, comme on pouvait s'y attendre dans cette zone riche en minéraux, mais cette concentration n'atteignait pas un niveau pouvant provoquer des toxicoses. La concentration de cadmium dans le rein, qui augmentait de façon significative avec l'âge, pourrait constituer un risque pour la consommation humaine, ce qui n'est pas surprenant. On a conclu que les caribous étaient morts de faim et que les métaux lourds n'avaient joué aucun rôle dans l'épisode de mortalité. Il faudrait effectuer des recherches plus poussées sur les différences régionales en minéraux afin de comprendre les mécanismes d'origine et de transport qui expliquent ces résultats et d'aborder comme il le faut les activités minières. Les épisodes de mortalité sont courants sur le versant Nord de l'Alaska et sont probablement liés à la famine, comme le décrit cet article, mais dans la plupart des cas ils ne font pas l'objet d'une enquête, même si l'activité industrielle récente est un sujet qui préoccupe de plus en plus certains résidents et gestionnaires locaux de la faune

Contributions and perspectives of Indigenous Peoples to the study of mercury in the Arctic

Arctic Indigenous Peoples are among the most exposed humans when it comes to foodborne mercury (Hg). In response, Hg monitoring and research have been on-going in the circumpolar Arctic since about 1991; this work has been mainly possible through the involvement of Arctic Indigenous Peoples. The present overview was initially conducted in the context of a broader assessment of Hg research organized by the Arctic Monitoring and Assessment Programme. This article provides examples of Indigenous Peoples' contributions to Hg monitoring and research in the Arctic, and discusses approaches that could be used, and improved upon, when carrying out future activities. Over 40 mercury projects conducted with/by Indigenous Peoples are identified for different circumpolar regions including the U.S., Canada, Greenland, Sweden, Finland, and Russia as well as instances where Indigenous Knowledge contributed to the understanding of Hg contamination in the Arctic. Perspectives and visions of future Hg research as well as recommendations are presented. The establishment of collaborative processes and partnership/co-production approaches with scientists and Indigenous Peoples, using good communication practices and transparency in research activities, are key to the success of research and monitoring activities in the Arctic. Sustainable funding for community-driven monitoring and research programs in Arctic countries would be beneficial and assist in developing more research/ monitoring capacity and would promote a more holistic approach to understanding Hg in the Arctic. These activities should be well connected to circumpolar/international initiatives to ensure broader availability of the information and uptake in policy development

Adaptation of mammalian host-pathogen interactions in a changing arctic environment

Many arctic mammals are adapted to live year-round in extreme environments with low winter temperatures and great seasonal variations in key variables (e.g. sunlight, food, temperature, moisture). The interaction between hosts and pathogens in high northern latitudes is not very well understood with respect to intra-annual cycles (seasons). The annual cycles of interacting pathogen and host biology is regulated in part by highly synchronized temperature and photoperiod changes during seasonal transitions (e.g., freezeup and breakup). With a warming climate, only one of these key biological cues will undergo drastic changes, while the other will remain fixed. This uncoupling can theoretically have drastic consequences on host-pathogen interactions. These poorly understood cues together with a changing climate by itself will challenge host populations that are adapted to pathogens under the historic and current climate regime. We will review adaptations of both host and pathogens to the extreme conditions at high latitudes and explore some potential consequences of rapid changes in the Arctic

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2–4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease