Interrogating cellular perception and decision making with optogenetic tools.

Optogenetics promises to deepen our understanding of how cells perceive and respond to complex and dynamic signals and how this perception regulates normal and abnormal function. In this study, we present our vision for how these nascent tools may transform our view of fundamental cell biological processes

A conceptual analytics model for an outcome-driven quality management framework as part of professional healthcare education

BACKGROUND: Preparing the future health care professional workforce in a changing world is a significant undertaking. Educators and other decision makers look to evidence-based knowledge to improve quality of education. Analytics, the use of data to generate insights and support decisions, have been applied successfully across numerous application domains. Health care professional education is one area where great potential is yet to be realized. Previous research of Academic and Learning analytics has mainly focused on technical issues. The focus of this study relates to its practical implementation in the setting of health care education. OBJECTIVE: The aim of this study is to create a conceptual model for a deeper understanding of the synthesizing process, and transforming data into information to support educators’ decision making. METHODS: A deductive case study approach was applied to develop the conceptual model. RESULTS: The analytics loop works both in theory and in practice. The conceptual model encompasses the underlying data, the quality indicators, and decision support for educators. CONCLUSIONS: The model illustrates how a theory can be applied to a traditional data-driven analytics approach, and alongside the context- or need-driven analytics approach

U.S. Small Farms: Decline and Persistence?

We use two comprehensive and representative USDA databases to assess the performance of small farms in the U.S. Farm production is shifting to much larger farms, and the number of small commercial farms is declining. Most large U.S. farms remain family-owned and operated enterprises, and most remain small businesses by U.S. standards. Small commercial farms tend to focus on three commodities: beef cattle, grains and oilseeds, and poultry. On average, large farm financial returns substantially exceed those on small farms, but the range of performance among small farms is quite wide. About one quarter of the nearly 800,000 small commercial farms show very good financial returns.small farms, structural change, farm income, Agricultural and Food Policy, Q12,

Near-bed hydrodynamics and turbulence below a large-scale plunging breaking wave over a mobile barred bed profile

Funded by The research presented in this paper is part of the SINBAD project. Grant Number: STW (12058) and EPSRC (EP/J00507X/1, EP/J005541/1)Peer reviewedPublisher PDFPublisher PD

Analysis of repeated high-intensity running performance in professional soccer

The aims of this study conducted in a professional soccer team were two-fold: to characterise repeated high-intensity movement activity profiles in official match-play; b) to inform and verify the construct validity of tests commonly used to determine repeated-sprint ability in soccer by investigating the relationship between the results from a test of repeated-sprint ability and repeated high-intensity performance in competition. High-intensity running performance (movement at velocities >19.8 km/h for a minimum of 1-s duration) in 20 players was measured using computerised time motion analysis. Performance in 80 French League 1 matches was analysed. In addition, 12 out of the 20 players performed a repeated-sprint test on a non-motorized treadmill consisting of 6 consecutive 6s sprints separated by 20s passive recovery intervals. In all players, the majority of consecutive high-intensity actions in competition were performed after recovery durations ≥61s, recovery activity separating these efforts was generally active in nature with the major part of this spent walking, and players performed 1.1±1.1 repeated high-intensity bouts (a minimum of 3 consecutive high-intensity with a mean recovery time ≤20s separating efforts) per game. Players reporting lowest performance decrements in the repeated-sprint ability test performed more high-intensity actions interspersed by short recovery times (≤20s, p<0.01 and ≤30s, p<0.05) compared to those with higher decrements. Across positional roles, central-midfielders performed a greater number of high-intensity actions separated by short recovery times (≤20s) and spent a larger proportion of time running at higher intensities during recovery periods while fullbacks performed the most repeated high-intensity bouts (statistical differences across positional roles from p<0.05 to p<0.001). These findings have implications for repeated high-intensity testing and physical conditioning regimens

Near-Bed Turbulent Kinetic Energy Budget Under a Large-Scale Plunging Breaking Wave Over a Fixed Bar

Hydrodynamics under regular plunging breaking waves over a fixed breaker bar were studied in a large-scale wave flume. A previous paper reported on the outer flow hydrodynamics; the present paper focuses on the turbulence dynamics near the bed (up to 0.10 m from the bed). Velocities were measured with high spatial and temporal resolution using a two component laser Doppler anemometer. The results show that even at close distance from the bed (1 mm), the turbulent kinetic energy (TKE) increases by a factor five between the shoaling, and breaking regions because of invasion of wave breaking turbulence. The sign and phase behavior of the time-dependent Reynolds shear stresses at elevations up to approximately 0.02 m from the bed (roughly twice the elevation of the boundary layer overshoot) are mainly controlled by local bed-shear-generated turbulence, but at higher elevations Reynolds stresses are controlled by wave breaking turbulence. The measurements are subsequently analyzed to investigate the TKE budget at wave-averaged and intrawave time scales. Horizontal and vertical turbulence advection, production, and dissipation are the major terms. A two-dimensional wave-averaged circulation drives advection of wave breaking turbulence through the near-bed layer, resulting in a net downward influx in the bar trough region, followed by seaward advection along the bar's shoreward slope, and an upward outflux above the bar crest. The strongly nonuniform flow across the bar combined with the presence of anisotropic turbulence enhances turbulent production rates near the bed

An expanded phylogeny of the Entodiniomorphida (Ciliophora : Litostomatea)

The Entodiniomorphida are a diverse and morphologically complex group of ciliates which are symbiotic within the digestive tracts of herbivorous mammals. Previous phylogenies of the group have exclusively considered members of one family, the Ophryoscolecidae, which are symbiotic within ruminants. We sought to improve understanding of evolution within the entodiniomorphs by expanding the range of ciliates examined to include the Cycloposthiidae and Macropodimidae (symbionts of equids and macropodids respectively). The entire SSU-rRNA gene was sequenced for 3 species, Cycloposthium edentatum, Macropodinium ennuensis and M. yalanbense, and aligned against 14 litostome species and 2 postciliodesmatophoran outgroup species. Cycloposthium was consistently grouped as the sister-taxon to the Ophryoscolecidae although support for this relationship was low. This suggests that there is more evolutionary distance between the Cycloposthiidae and Ophryoscolecidae than previously inferred from studies of gross morphology, cell ontogeny or ultrastructure. In contrast, Macropodinium did not group with any of the entodiniomorphs, instead forming the sister group to the entire Trichostomatia (Entodiniomorphida + Vestibuliferida). This early diverging position for the macropodiniids is concordant with their morphology and ontogeny which failed to group the family with any of the entodiniomorph suborders. The currently accepted classification of the Trichostomatia is thus deficient and in need of review