Canopy management, leaf fall and litter quality of dominant tree species in the banana agroforestry system in Uganda

Small-scale farmers in the banana-coffee agro-zone of Central Uganda plant andmaintain trees to provide a range of benefits. However, the impact of trees on soil fertility and crop yields is small. On many farms, trees exist in infinite numbers, compositions, with no proper spacing, sequencing and canopy management  recommendations. The objectives of this study were to: quantify and compare leaf fall and leaf litter biomass for the dominant tree species subjected to different pruning regimes and during different seasons and determine the nutrient  concentrations in leaf litter materials in order to assess their potential to improve soil fertility. A survey was conducted on 30 randomly selected farms to examine and  document the tree species. Four tree species were dominant; Ficus natalensis (15.3%), Albizia coriaria (10.2%), Artocarpus  heterophyllus (10.0%) and Mangifera indica (9.2%). Experiments such as canopy pruning, collecting leaf fall and weights of pruned leaf biomass were established for the four dominant trees on five randomly selected farms. A total of 120 trees were subjected to three pruning regimes (0%, 25% and 50%). Litter traps (1 m × 1 m) were placed 2 m from the tree trunk to collect falling leaves. Leaf biomass was collected from pruned branches and eighed. Leaf samples were collected from pruned leaves and a composite sample analysed for carbon and nutrient concentrations. Results showed that 40 tree and shrub species belonging to 21 families existed on farms. Leaf fall was highest in the dry season and A. Heterophyllus registered the highest amount. Leaf biomass from pruned trees was heaviest for M. indica (42.4 ±3 kg) in the wet season and at the 50% pruning regime (45.7± 2.1kg). Total  nutrient concentrations were in the order A. coriaria>F. natalensis>A.heterophyllus>M.indica. We concluded that F. natalensis and A. Coriaria should be integrated more into the agroforestry system to improve soil fertility. A.heterophyllus and M.indica should be planted on farm boundaries. The 50% pruning regime was the best and should be carried out during the wet season.Key words: Tree species, canopy management, leaf fall, nutrient concentrations, soil fertilit

Farmers’ knowledge of the banana (Musa Sp.) agroforestry systems in Kiboga District, Central Uganda

Banana is a major food and cash crop in Uganda, particularly in the central and southwestern regions. However, production is still below attainable yields and at the same time declining due to a number of reasons, with low soil fertility being paramount. Few farmers use inorganic fertilizers, with majority of them relying mainly on organic supplements including integration of trees/shrubs. However, trees and shrubs exist on banana farms in undefined numbers and composition, with no spacing and canopy management recommendations. Also, there is limited information on farmers’ knowledge on these systems. A survey was, therefore, conducted on 70 randomly selected farms in Kiboga district, central Uganda to assess farmers’ knowledge of, as well as identify trees and shrubs species and banana cultivars in their agro-ecologies. A total of 1,558 trees and shrubs belonging to 40 species and 21 families were recorded in the study area (52 trees/shrubs per banana plantation). These were dominated by Jackfruit, Artocarpus heterophyllus (15%), Natal fig, Ficus natalensis (10%), Albizia, Albizia coriaria (10%) and mango, Mangifera indica (9%). Further, 1,779 banana mats belonging to nine (9) cultivars and three (3) genome groups were observed growing underneath these four commonest tree species (2.6 banana mats per tree). The highest number of banana mats was observed growing underneath F. natalensis (54%) and A. coriaria (42%). Most (87%) observed banana cultivars belonged to the cooking East African Highland Banana (Musa AAA-EAHB) and dominated by Ndibwabalangira (21%). Farmers reported 25 benefits they derive from banana-trees/shrubs integration; with shade and firewood (38%), as well as income and timber (35%) being the main benefits reported. They also reported that the highest percentage of benefits was derived from F. natalensis (44%) and A. coriaria (32%). Of the four (4) most prevalent tree species, A. coriaria (100%) and F. natalensis (96%) were reported as good companion plant to bananas, whereas, A. heterophyllus (99%) and M. indica (97%) were regarded as bad companion plants. Farmers preferred a tree that allows light penetration (96%), is compatible with bananas and other crops (87%) and has easily decomposable leaves (83%). Therefore, F. natalensis and A. coriaria should be integrated in banana agrosystems for soil fertility improvement whereas; A. heterophyllus and M. indica be planted on farm boundaries. However, the best-bet spacing and pruning regimes for these trees should be determined to minimize the negative attributes as much as possible.Keywords: Albizia coriaria, Artocarpus heterophyllus, banana cultivars, benefits, companions, Ficus natalensis, Mangifera indica, tree-specie

Antimicrobial resistance patterns of phenotype Extended Spectrum Beta-Lactamase producing bacterial isolates in a referral hospital in northern Tanzania

Background: Production of Extended Spectrum Beta-Lactamase (ESBL) by bacteria is a chronic problem in a health care set up. In order to have adequate information for treatment of bacterial infections especially ESBL producing isolates, it is crucial to understand the trends in the antibiotic-resistance pattern, occurrence and their geographical spread.  The objective of this study was to determine the antimicrobial resistance pattern among phenotype ESBL producing isolates in northern Tanzania.Methods: From July 2013 to January 2014, urine, pus and blood samples were collected from patients suspected to have bacterial infections at Kilimanjaro Christian Medical Centre in Moshi, Tanzania. The isolates were identified based on standard laboratory procedures. Antimicrobial susceptibility tests were carried out using various antimicrobial discs as per the recommendations of Clinical Laboratory Standard Institute.Results: A total of 330 specimens were collected. They consisted of 46 urine, 264 pus (from wound) and 20 blood samples. Among isolated bacteria, ESBL producers were 29.7% (98) and non-producers were 70.5% (232).  Escherichia coli and Klebsiella pneumoniae were the most isolated bacteria and dominant ESBL producers.  ESBL production was highly associated with moderate condition at discharge and longer periods of admission. More than 60% of the ESBL producing E. coli were resistant to ceftazidime, cefpodoxime, cefotaxime, amoxycilin, ciprofloxacin, and gentamycin. More than 80% of ESBL producing K. pneumonia and Proteus mirabilis were resistant to ceftazidime and cefotaxime. Fifty four percent of ESBL producing K. pneumonia were resistant to gentamycin.Conclusion: This study shows that ESLB phenotypes among Gram-negative bacteria are common among patients attending a tertiary hospital in northern in Tanzania. The findings suggest that clinical microbiology laboratories should take into account the diagnosis of ESBL producers in order to define the degree of the problem so as to establish a proper treatment protocol

Canopy management, leaf fall and litter quality of dominant tree species in the banana agroforestry system in Uganda

Small-scale farmers in the banana-coffee agro-zone of Central Uganda plant andmaintain trees to provide a range of benefits. However, the impact of trees on soil fertility and crop yields is small. On many farms, trees exist in infinite numbers, compositions, with no proper spacing, sequencing and canopy management  recommendations. The objectives of this study were to: quantify and compare leaf fall and leaf litter biomass for the dominant tree species subjected to different pruning regimes and during different seasons and determine the nutrient  concentrations in leaf litter materials in order to assess their potential to improve soil fertility. A survey was conducted on 30 randomly selected farms to examine and  document the tree species. Four tree species were dominant; Ficus natalensis (15.3%), Albizia coriaria (10.2%), Artocarpus  heterophyllus (10.0%) and Mangifera indica (9.2%). Experiments such as canopy pruning, collecting leaf fall and weights of pruned leaf biomass were established for the four dominant trees on five randomly selected farms. A total of 120 trees were subjected to three pruning regimes (0%, 25% and 50%). Litter traps (1 m × 1 m) were placed 2 m from the tree trunk to collect falling leaves. Leaf biomass was collected from pruned branches and eighed. Leaf samples were collected from pruned leaves and a composite sample analysed for carbon and nutrient concentrations. Results showed that 40 tree and shrub species belonging to 21 families existed on farms. Leaf fall was highest in the dry season and A. Heterophyllus registered the highest amount. Leaf biomass from pruned trees was heaviest for M. indica (42.4 ±3 kg) in the wet season and at the 50% pruning regime (45.7± 2.1kg). Total  nutrient concentrations were in the order A. coriaria>F. natalensis>A.heterophyllus>M.indica. We concluded that F. natalensis and A. Coriaria should be integrated more into the agroforestry system to improve soil fertility. A.heterophyllus and M.indica should be planted on farm boundaries. The 50% pruning regime was the best and should be carried out during the wet season.Key words: Tree species, canopy management, leaf fall, nutrient concentrations, soil fertilit

Deciphering the Complex Distribution of Human Immunodeficiency Virus Type 1 Subtypes among Different Cohorts in Northern Tanzania.

Increased understanding of the genetic diversity of HIV-1 is challenging but important in the development of an effective vaccine. We aimed to describe the distribution of HIV-1 subtypes in northern Tanzania among women enrolled in studies preparing for HIV-1 prevention trials (hospitality facility-worker cohorts), and among men and women in an open cohort demographic surveillance system (Kisesa cohort). The polymerase encompassing partial reverse transcriptase was sequenced and phylogenetic analysis performed and subtype determined. Questionnaires documented demographic data. We examined factors associated with subtype using multinomial logistic regression, adjusted for study, age, and sex. Among 140 individuals (125 women and 15 men), subtype A1 predominated (54, 39%), followed by C (46, 33%), D (25, 18%) and unique recombinant forms (URFs) (15, 11%). There was weak evidence to suggest different subtype frequencies by study (for example, 18% URFs in the Kisesa cohort versus 5-9% in the hospitality facility-worker cohorts; adjusted relative-risk ratio (aRR) = 2.35 [95% CI 0.59,9.32]; global p = 0.09). Compared to men, women were less likely to have subtype D versus A (aRR = 0.12 [95% CI 0.02,0.76]; global p = 0.05). There was a trend to suggest lower relative risk of subtype D compared to A with older age (aRR = 0.44 [95% CI 0.23,0.85] per 10 years; global p = 0.05). We observed multiple subtypes, confirming the complex genetic diversity of HIV-1 strains circulating in northern Tanzania, and found some differences between cohorts and by age and sex. This has important implications for vaccine design and development, providing opportunity to determine vaccine efficacy in diverse HIV-1 strains

HIV-1 pol Diversity among Female Bar and Hotel Workers in Northern Tanzania

A national ART program was launched in Tanzania in October 2004. Due to the existence of multiple HIV-1 subtypes and recombinant viruses co-circulating in Tanzania, it is important to monitor rates of drug resistance. The present study determined the prevalence of HIV-1 drug resistance mutations among ART-naive female bar and hotel workers, a high-risk population for HIV-1 infection in Moshi, Tanzania. A partial HIV-1 pol gene was analyzed by single-genome amplification and sequencing in 45 subjects (622 pol sequences total; median number of sequences per subject, 13; IQR 5–20) in samples collected in 2005. The prevalence of HIV-1 subtypes A1, C, and D, and inter-subtype recombinant viruses, was 36%, 29%, 9% and 27%, respectively. Thirteen different recombination patterns included D/A1/D, C/A1, A1/C/A1, A1/U/A1, C/U/A1, C/A1, U/D/U, D/A1/D, A1/C, A1/C, A2/C/A2, CRF10_CD/C/CRF10_CD and CRF35_AD/A1/CRF35_AD. CRF35_AD was identified in Tanzania for the first time. All recombinant viruses in this study were unique, suggesting ongoing recombination processes among circulating HIV-1 variants. The prevalence of multiple infections in this population was 16% (n = 7). Primary HIV-1 drug resistance mutations to RT inhibitors were identified in three (7%) subjects (K65R plus Y181C; N60D; and V106M). In some subjects, polymorphisms were observed at the RT positions 41, 69, 75, 98, 101, 179, 190, and 215. Secondary mutations associated with NNRTIs were observed at the RT positions 90 (7%) and 138 (6%). In the protease gene, three subjects (7%) had M46I/L mutations. All subjects in this study had HIV-1 subtype-specific natural polymorphisms at positions 36, 69, 89 and 93 that are associated with drug resistance in HIV-1 subtype B. These results suggested that HIV-1 drug resistance mutations and natural polymorphisms existed in this population before the initiation of the national ART program. With increasing use of ARV, these results highlight the importance of drug resistance monitoring in Tanzania