Proceedings of EADPH Pre -Congress Workshop held on Wednesday, 11 September 2019 at Het Pand, University of Ghent, Belgium. Best practices in dental curricula development – a follow-up to the 2018 EADPH Pre-Congress Workshop

This document reports the proceedings of a workshop held in Ghent on 11 September 2019, the day before the annual congress of the European Association of Dental Public Health. It is taken directly from the transcription of an audio recording. The workshop consisted of eight short presentations which described curriculum changes and examples of inter-professional education and practice involving dental public health in European countries. The presentations were followed by discussions in four small working groups and reports from each group which highlighted achievements, barriers and challenge

Exploring the three PIPs and three TIPs of grapevine for transport of water and atypical substrates through heterologous expression in aqy-null yeast

Aquaporins are membrane channels that facilitate the transport of water and other small molecules across the cellular membranes. We examined the role of six aquaporins of Vitis vinifera (cv. Touriga nacional) in the transport of water and atypical substrates (other than water) in an aqy-null strain of Saccharomyces cerevisiae. Their functional characterization for water transport was performed by stopped-flow fluorescence spectroscopy. The evaluation of permeability coefficients (Pf) and activation energies (Ea) revealed that three aquaporins (VvTnPIP2;1, VvTnTIP1;1 and VvTnTIP2;2) are functional for water transport, while the other three (VvTnPIP1;4, VvTnPIP2;3 and VvTnTIP4;1) are non-functional. TIPs (VvTnTIP1;1 and VvTnTIP2;2) exhibited higher water permeability than VvTnPIP2;1. All functional aquaporins were found to be sensitive to HgCl2, since their water conductivity was reduced (24–38%) by the addition of 0.5 mM HgCl2. Expression of Vitis aquaporins caused different sensitive phenotypes to yeast strains when grown under hyperosmotic stress generated by KCl or sorbitol. Our results also indicate that Vitis aquaporins are putative transporters of other small molecules of physiological importance. Their sequence analyses revealed the presence of signature sequences for transport of ammonia, boron, CO2, H2O2 and urea. The phenotypic growth variations of yeast cells showed that heterologous expression of Vitis aquaporins increased susceptibility to externally applied boron and H2O2, suggesting the contribution of Vitis aquaporins in the transport of these speciesinfo:eu-repo/semantics/publishedVersio

Crystal Structure of a Yeast Aquaporin at 1.15 Å Reveals a Novel Gating Mechanism

Atomic-resolution X-ray crystallography, functional analyses, and molecular dynamics simulations suggest a novel mechanism for the regulation of water flux through the yeast Aqy1 water channel

Proceedings of EADPH Pre -Congress Workshop held on Wednesday, 11 September 2019 at Het Pand, University of Ghent, Belgium. Best practices in dental curricula development – a follow-up to the 2018 EADPH Pre-Congress Workshop

This document reports the proceedings of a workshop held in Ghent on 11 September 2019, the day before the annual congress of the European Association of Dental Public Health. It is taken directly from the transcription of an audio recording. The workshop consisted of eight short presentations which described curriculum changes and examples of inter-professional education and practice involving dental public health in European countries. The presentations were followed by discussions in four small working groups and reports from each group which highlighted achievements, barriers and challenge