new route for tizanidine administration: a pharmacokinetics and light microscope autoradiography study

Spasticity could represent a complication of several central nervous system (CNS) disorders. Multiple sclerosis and painful paroxysmal syndromes (e.g. trigeminal neuralgia) are pathologies in which anti-spastic drugs are used to a greater extent . Baclofen, tizanidine (TIZ), benzodiazepines, dantrolene, and, more recently, gabapentin are the pharmacological agents more widely used. Baclofen, TIZ, benzodiazepines, gabapentin, clonidine but not dantrolene are active on CNS. The alpha2 adrenoceptor agonist TIZ is one of the most eff ective and largely used anti-spastic drugs. Oral treatment is the only route of anti-spastics administration, although it may cause problems of bioavailability and/or compliance in spastic patients with impaired deglutition. This study was designed to assess the possibility of develop a new route of administration of TIZ and to identify its targets in the spinal cord. New Zealand rabbits were treated with oral (OR , n=6), intramuscular (IM, n=6) or intranasal (IN, n=6) TIZ (3.2 mg/kg/day). Plasma concentration was measured by HPLC on samples collected at 0, 30, 60, 90, 120 and 480 min after treatment. Curves of average concentrations of TIZ vs. time were constructed. In plasma, TIZ reached a peak between 45-65 min after administration. Cmax was in the range of 268.33–1213.64 ng/ml for IM treatment, of 73.95–135.92 ng/ml for IN treatment and of 16.86–857.25 ng/ml for OR treatment. After pharmacokinetic studies, diff erent spinal cord tracts were removed and used for radioligand binding assay and autoradiography. Using [3H]-RX821002 ([3H]-RX) (0.1-14nM), the Kd and Bmax for every spinal cord segment were calculated. The non-specifi c binding was obtained with 100μM of (-)-epinephrine. [3H]-RX sites were accumulated in the superfi cial laminae of dorsal horn. Dense [3H]-RX binding in control was seen over the superfi cial dorsal horn (laminae I-II) and centrally located lamina X. The ventral horn showed moderate levels of binding. [3H]-RX was displaced after TIZ treatment using diff erent administration routes. The heaviest accumulation of silver grains (lowest displacement of TIZ) occurred after OR administration of the compound, the lowest after IM administration. These results suggest that both IM and IN administration of TIZ may represent routes of administration of the drug alternative to the OR one. Considering predictable adverse eff ects of IM treatment, the IN administration could represent the elective route to administrate this kind of drugs

Design-led intervention for active behaviour to tackle youth’s sedentary behaviour

Physical inactivity has been a predominant cause of major chronic health problems. In 2020 the World Health Organization (WHO) issued revised guidelines on physical activity and sedentary behavior for children to encourage a more active lifestyle. However, due to the growing trends of digital culture, children at a young age are exposed to increased screen-related sedentary activities. We add to this endeavor by considering creative ways to promote an active lifestyle to reduce the risks associated this. In this paper we report on a design-led intervention that was applied to increase physical activity and reduce sedentary time in the home environment. We also discuss the effectiveness of this intervention on regulating changed behavior. Data were gathered from 20 households' participants over 12 weeks using a novel prototype called the Knudgebox. This is a program where physical activities are a condition to have screen access. This prototype was designed to facilitate active self-regulation to reduce sedentary behavior instead of stimulating a new behavior change through extrinsic benefits. Our results show that an increase in physical activities and a decrease in screen time can be achieved using the Knudgebox. We identified new behavioral patterns and insights regarding how design elements affected their determinants in taking changed behavior from triggered to self-regulation

Animal Cruelty: A Review

Animal Cruelty cases make headlines around the world every day, whether it’s the person who kills the neighbour’s cat, the hoarder of sick and dying animals or the family whose freezing, starving dog is tied up outside in the middle of the winter. Animal Cruelty has several types which may be one of Simple Neglect, Gross Neglect, Intentional Abuse, Animal Hoarding, Organized Abuse, Ritualistic Abuse or Animal Sexual Assault. These cases of Cruelty cannot be overlooked as it has proven to have severe implications ranging from the fact that it is linked to other crimes, to the factual truth that these practises inflict great pain on these animals that have nobody to speak for them. Combating this menace of Animal Cruelty is a Journey that must be undertaken and everybody including the Government, NGOs and even Culture has a major role to play in this.  This review looks at Animal Cruelty in the world while paying a little more attention to Nigeria

Dark Energy or Apparent Acceleration Due to a Relativistic Cosmological Model More Complex than FLRW?

We use the Szekeres inhomogeneous relativistic models in order to fit supernova combined data sets. We show that with a choice of the spatial curvature function that is guided by current observations, the models fit the supernova data almost as well as the LCDM model without requiring a dark energy component. The Szekeres models were originally derived as an exact solution to Einstein's equations with a general metric that has no symmetries and are regarded as good candidates to model the true lumpy universe that we observe. The null geodesics in these models are not radial. The best fit model found is also consistent with the requirement of spatial flatness at CMB scales. The first results presented here seem to encourage further investigations of apparent acceleration using various inhomogeneous models and other constraints from CMB and large structure need to be explored next.Comment: 6 pages, 1 figure, matches version published in PR

Evaluation of children’s screen viewing time and parental role in household context

Limited screen time has been recommended by the American Academy of Pediatrics for all children and no screen time for children under 2 years of age. With the advancement in technology, the prevalence and detrimental effects of excess screen time on children has become a global problem that can lead to health issues such as obesity and other cardiovascular diseases among both adults and children. This article examines the drivers of screen-related sedentary behavior within the home context and reports on parents’ attitude in supporting children’s associated behavior. The study implemented a mixed method approach of online questionnaire and face-to-face interviews. A total of 140 questionnaires, 10 semistructured interviews, and responses from parents were used to evaluate children’s behavior toward screen use and parents’ perception. The analysis of the article has shown that parents are concerned about their children’s screen time during weekends and holidays with a significant correlation between the number of hours their children spend on on-screen activities and their level of concern (P < .01). Also, for the same P value (P < .01), it has been found that there is a statistically significant positive relationship between children’s screen time and their food and beverages consumption. Children’s use of screen technology is becoming a challenge for parents as they present barriers to healthy physical activities. From the parents’ viewpoint, there is a need to change the screen use habit and this requires an appropriate intervention that promotes gratifying measures to induce more active behaviors to displace screen viewing

Persistence of onchocerciasis in villages in Enugu and Ogun states in Nigeria following many rounds of mass distribution of ivermectin

BACKGROUND: Onchocerciasis is endemic in most local government areas (LGAs) in Enugu and Ogun states. Most meso- and hyper-endemic LGAs have received many rounds of ivermectin mass drug administration (MDA). This study aimed to determine the current prevalence of onchocerciasis in villages in Enugu and Ogun states that were formerly highly endemic and to assess progress toward elimination of the infection in areas believed to be at high risk for persistence. METHODS: Cross-sectional community surveys were conducted 8 to 12 months after the last round of MDA in 16 villages (6 in Enugu state and 10 in Ogun state) in individuals aged ≥ 18 years. Study participants were examined for the presence of palpable subcutaneous nodules. Skin snips from the posterior iliac crests were used to assess microfiladermia (Mf) prevalence and density. RESULTS: 643 subjects were palpated for nodules and 627 individuals (225 in Enugu state; 402 in Ogun state) provided skin snips. Nodule prevalence in the study villages ranged from 42 to 66.7% in Enugu state and from 0 to 25.0% in Ogun state. Mf prevalence in the Enugu and Ogun study villages ranged from 32 to 51.1% and 0 to 28.6%, respectively. Geometric mean skin Mf density in surveyed Enugu state villages ranged between 1 and 3.1 Mf/mg; these values were \u3c 1 Mf/mg in all but one community in Ogun state villages. CONCLUSION: Results from this study show that onchocerciasis persists in adults in many villages in Enugu and Ogun states despite many prior rounds of ivermectin MDA. Prevalence was higher in villages surveyed in Enugu than in Ogun. Low Mf densities suggest the MDA program is working well to reduce disease, but more time will be required to reach the elimination goal

Characterizing the weather band variability of the Texas shelf current

Considering the benefits of understanding the circulation patterns of the shelf, it is not surprising that there are numerous studies of the Texas Shelf circulation patterns. Given that previous studies were focused on the low-frequency variability of the circulation which is upcoast (northeast flow) in the summer and downcoast (southwest flow) especially on the inner shelf in the non-summer seasons, this study investigates the weather band (2–15 days) variability of the Texas Shelf near-surface circulation pattern. Current meter data at 1.5 m below the sea surface from the inner, mid, and outer shelves were analyzed. This study demonstrated that there are high-frequency current reversals within the weather band in each season. From the estimated persistence of the currents during reversals, the inner and mid shelf currents are predominantly downcoast in the non-summer seasons and upcoast in the summer season whereas the outer shelf currents are mostly upcoast all year round. The Wavelets analysis of the currents revealed that most of the variabilities on the inner and mid shelf regions were within the 4-12-day band whereas on the outer shelf the dominant variability was within the 3–8-day band. From the cross-spectra analysis of both the currents and wind data, it was determined that the influence of the wind was more dominant on the inner and mid shelf regions at the 8–15-day band than on the outer shelf where the contribution of the wind is prevalent at the 2–4-day band

Measurements of Terrestrial Gamma Dose Rate Distributions along Idiroko Road, Ota, Ogun State, Nigeria: Health Implications on Roadside Dwellers

An extensive study concerning the terrestrial gamma dose rates of the surface soils from Canaanland entrance Road, Ota to Eleha Complex along Idiroko Road, Ota has been carried out to cover the road running across both areas. The In-situ measurements of gamma dose rates and radionuclides were carried out using the Gamma detector (Super Spec RS 125). The result shows that the average outdoor gamma dose rate in the study area is 73.57 nGyh-1. The average values of the radionuclides obtained were 32.9 Bq/kg for 238U, 68.4 Bq/kg for 232Th, and 328.7 Bq/kg for 40K. The background dose rate of 113.3 nGy/h was found in station 9 and is twice higher than the recommended permissible limit of 59 nGy/h suggested by UNCSEAR, 2000, and ICRP, 1999, respectively. Results were obtained based on radiological parameters for the outdoor absorbed dose rate (Dout) with a mean value of 73.57 nGyh-1, the indoor absorbed dose rate (Din) with a mean value of 131.84 nGyh-1, the annual effective dose rate (AEDR) with a mean value of 0.74, the radium equivalent (Raeq) with a mean value of 156.0, the internal hazard index (Hin) with a mean value of 0.51, the external hazard index (Hex) with a mean value of 0.42 and the excess lifetime cancer risk (ELCR) with a mean value of 2.57. Similarly, results obtained from estimated radiological parameters show that the station with the highest value for radium equivalent (Raeq), external hazard index (Hex), internal hazard index (Hin), and annual effective dose rate (AEDR) is Station 9 at 237.91 Bq/Kg, 0.64, 0.72 and 1.13 mSvy-1 respectively while the station with the lowest value for radium equivalent (Raeq), external hazard index (Hex), internal hazard index (Hin) and annual effective dose rate (AEDR) is Station 17 at 85.58 Bq/Kg, 0.23, 0.27 and 0.40 mSvy-1 respectively. This study will serve as a guide on assessing the road construction materials on radioactive contents before application to reduce the exposure risks to the populace along the busy roadsides

Rampant introgressive hybridization in Pogoniulus tinkerbirds (Piciformes: Lybiidae) despite millions of years of divergence

Incomplete reproductive isolation between related species of birds at contact zones is increasingly being documented. Such hybridization typically occurs between sister taxa that diverged in relatively recent times, and hybrids are most often identified based on their intermediate phenotypic characteristics and, increasingly, through genetic admixture analysis. When species have been diverging over relatively longer time scales, prezygotic isolation barriers are expected to evolve, precluding maladaptive interbreeding. Here, we examine the extent of introgressive hybridization in a pair of African barbets, the yellow-fronted tinkerbird (Pogoniulus chrysoconus extoni) and the red-fronted tinkerbird (Pogoniulus pusillus pusillus), which were not previously known to interbreed, across a contact zone in Southern Africa. Although there were significant differences in the coloration of plumage between the species, we found a pattern of extensive admixture in and around the contact zone. Nonetheless, the two species appear to have diverged > 4 Mya and might not even be sister taxa, suggesting that time of divergence alone might not be sufficient for the evolution of prezygotic reproductive barriers. Significantly more phenotypically red-fronted individuals had a P. c. extoni (yellow-fronted) genetic background than vice versa, suggesting possible asymmetry in mate preferences. Sexual selection may thus play a role in breaking down species barriers despite the extent of genetic divergence.A Marie Curie International Reintegration Grant (A.N.G.K.) and the A. P. Leventis Ornithological Research Institute (E.C.N. and B.O.O.).https://academic.oup.com/biolinnean2020-04-03hj2019Mammal Research Institut