Bioremediation of PCB-contaminated marine sediments: From identification of indigenous dehalorespirers to enhancement of microbial reductive dechlorination

Marine sediments are the main accumulation reservoir of organic recalcitrant pollutants such as polychlorinated biphenyls (PCBs). In the anoxic conditions typical of these sediments, anaerobic bacteria of the phylum Chloroflexi are able to attack these compounds in a process called microbial reductive dechlorination. Such activity and members of this phylum were detected in PCB-impacted sediments of the Venice Lagoon. The aim of this work was to investigate microbial reductive dechlorination and design bioremediation approaches for marine sediments of the area. Three out of six sediment cultures from different sampling areas exhibited dechlorination activities in the same conditions of the site and two phylotypes (VLD-1 and VLD-2) were detected and correlated to this metabolism. Biostimulation was tested on enriched dechlorinating sediment cultures from the same site using five different electron donors, of which lactate was the best biostimulating agent; complementation of microbial and chemical dechlorination catalyzed by biogenic zerovalent Pd nanoparticles was not effective due to sulfide poisoning of the catalyst. A new biosurfactant-producing strain of Shewanella frigidimarina was concomitantly obtained from hydrocarbon-degrading marine cultures and selected because of the low toxicity of its product. All these findings were then exploited to develop bioremediation lab-scale tests in shaken reactors and static microcosms on real sediments and water of the Venice lagoon, testing i) a bioaugmentation approach, with a selected enriched sediment culture from the same area, ii) a biostimulation approach with lactate as electron donor, iii) a bioavailability enhancement with the supplementation of the newly-discovered biosurfactant, and iv) all possible combinations of the afore-mentioned approaches. The best bioremediation approach resulted to be a combination of bioaugmentation and bioremediation and it could be a starting point to design bioremediation process for actual marine sediments of the Venice Lagoon area

Partial Discharge Phenomena in Electrical Machines for the More Electrical Aircraft. Part II: Impact of Reduced Pressures and Wide Bandgap Devices

This paper focuses on the inception of partial discharges within the insulation system of electrical actuators used for the More Electrical Aircraft (MEA). Since these machines should operate in the absence of Partial Discharges (PDs), the dependence of the PD Inception Voltage (PDIV) on voltage impulses typical of wide bandgap (SiC) devices is investigated at both 1 bar, reduced pressures close to those typical of aircraft cruising altitudes (150 mbar – 200 mbar) or lower (down to 5 mbar). Propagation issues are not dealt with here as results were obtained working on insulation models consisting of couples of wires twisted together (twisted pairs), thus knowing exactly the potential differences between all points of the insulation model. The results show that the rise times and the switching frequencies associated with wide bandgap devices have little impact on the PDIV. A model able to predict the PDIV of the turn/turn insulation of random wound motors (the most vulnerable part of the insulation) at different pressures is proposed. The model is also able to deal with temperature changes, with limitations that depend on the type on insulation systems

Market Efficiency, Trading Institutions and Information Mirages: evidence from an experimental asset market

We investigate traders’ behaviour in an experimental asset market where uninformed agents cannot be sure about the presence of insiders. In this framework we compare two trading institutions: the continuous double auction and the call market. The purpose of this comparison is to test which of the two trading mechanisms performs better in disseminating the information and in promoting a convergence towards the efficient equilibrium price. Furthermore, we aim to determine which of the two trading institutions is more likely to promote a higher level of informational market efficiency. In a framework where the presence of insiders is neither certain nor common knowledge, inspired by Plott and Sunder (1982) and Camerer and Weigelt (1991), we first test whether a discrete time mechanism of trading, like the call market, might be able to prevent the occurrence of information mirages and promote a greater level of efficiency when no inside information is in the market. Second, we also compare the efficiency of the two trading institutions during periods when insiders are present in the market

Market Efficiency, Trading Institutions and Information Mirages: evidence from an experimental asset market

We investigate traders’ behaviour in an experimental asset market where uninformed agents cannot be sure about the presence of insiders. In this framework we compare two trading institutions: the continuous double auction and the call market. The purpose of this comparison is to test which of the two trading mechanisms performs better in disseminating the information and in promoting a convergence towards the efficient equilibrium price. Furthermore, we aim to determine which of the two trading institutions is more likely to promote a higher level of informational market efficiency. In a framework where the presence of insiders is neither certain nor common knowledge, inspired by Plott and Sunder (1982) and Camerer and Weigelt (1991), we first test whether a discrete time mechanism of trading, like the call market, might be able to prevent the occurrence of information mirages and promote a greater level of efficiency when no inside information is in the market. Second, we also compare the efficiency of the two trading institutions during periods when insiders are present in the market

Do Markets (Institutions) Drive Out Lemmings or Vice Versa?

We investigate, by mean of a lab experiment, a market inspired by two strands of literature on one hand we have herd behaviour in non-market situations, and on the other hand aggregation of private information in markets. The former suggests that socially undesirable herd behaviour may result when information is private; the latter suggests that socially undesirable behaviour may be eliminated through the market. As the trading mechanism might be a compounding factor, we investigate two kinds of market mechanism: the double auction, where bids, asks and trades take place in continuous time throughout a trading period; and the clearing house, where bids and asks are placed once in a trading period, and which are then cleared by an aggregating device. As a main result, this paper shows that double auction markets are, in several instances, superior to clearing house markets in terms of informational efficiency. Moreover, the employed trading institutions do not exhibit significant differences in both market volume and price volatility

Partial Discharges in Electrical Machines for the More Electric Aircraft—Part I: A Comprehensive Modeling Tool for the Characterization of Electric Drives Based on Fast Switching Semiconductors

This paper proposed a modelling approach for the comprehensive analysis of high-frequency challenges in electrical drives designed for aerospace applications, in particular the overvoltage at the machine terminals and the voltage distribution within windings. After a separate description of the models for the estimation of these insulation stress sources, the combined model was detailed. The main benefit of developing a combined, flexible and comprehensive tool is that both overvoltage at machine terminals and uneven voltage distribution can be calculated simultaneously, without neglecting the voltage overshoot when estimating the voltage distribution (and vice versa). In fact, an accurate calculation of the terminal overvoltage is necessary to provide a good estimation of the voltage within winding turns since its waveform shape can be quite different with respect to the converter output, even with cables of a few meters. A case study based on a real aerospace application was considered to investigate the model validity and accuracy. Experimental results were performed on a complete system comprising a SiC-based converter, a connecting cable and a machine stator, proving the simulation model accuracy in terms of peak voltages of both the line-to-line terminal voltage and the turn voltage distribution across the first turns, which are the most relevant quantities for the sake of this study as well as for the investigations of the subsequent companion papers. In the forthcoming papers, the effects of different rise times and cable lengths on the inception of partial discharges will be investigated through fast parametric simulation carried out using the proposed combined model. The feasibility of using conventional insulation systems for aircraft applications using SiC drives fed by a ±270 V DC bus voltage will be discussed, with the aim of signaling and finding solutions to improve the overall reliability

Significance of anti-HB levels below 10 IU/L after vaccination against hepatitis B in infancy or adolescence: an update in relation to sex

Hepatitis B vaccination (three-dose series) induces long-term immunity, but it is not uncommon to find antibody levels below 10 IU/L long after vaccination. However, the majority of the subjects with low antibody levels have a prompt response to a booster dose. A population of 10,294 students at Padua University Medical School, who were subjected to hepatitis B vaccination during infancy or adolescence according to the law, was tested for the presence of anti-HBs, usually during the first year of matriculation. Among the students offered a booster dose, 1,030 were vaccinated, and the antibody titre was re-tested. The present research provides further evidence from a larger number of students (1,030) that an anti-HB level higher than 2 IU/L is predictive of a prompt response to a booster. There are also differences related to sex. The results clearly confirm that an antibody titre equal to or greater than 2 IU/L is enough to prompt a response after a booster dose, even several years after the initial vaccination cycle, and to predict effective immune protection. The length of the interval between the booster/post-booster analyses increases the probability of finding a low response to the booster; furthermore, females show a more rapid response to the booster than males. The importance for healthcare workers of measuring the antibody titre four weeks after a booster is highlighted, and the results suggest that females have a better response than males to booster vaccination

Optimization of washing conditions with biogenic mobilizing agents for marine fuel-contaminated beach sands

Washing is a rapid and effective treatment to remediate contaminated sands impacted by oil spills, although synthetic additives used to increase extraction efficiency may cause additional pollution issues due to their intrinsic toxicity and very often low biodegradability. In this study, different biogenic mobilizing agents (soybean lecithins, cyclodextrins, cholic acids, plant-derived cleaners, rhamnolipids and sophorolipids) were tested in the washing of beach sands artificially contaminated with the Intermediate Fuel Oil IFO-180. Among these, a de-oiled soybean lecithin (SL-1), hydroxypropyl-beta-cyclodextrins (HPB-CD) and sophorolipids (SR) achieved hydrocarbon removals close to those attained with the synthetic surfactant Triton X-100 (TX) in preliminary washing tests carried out at constant mixing rate, water/sand ratio and IFO-180 contamination level using agents concentrations close to their critical micelle concentration (0.1% and 1% w/v for microbial and non-microbial agents, respectively). The effects of agent concentration, water/sand ratio, mixing rate and IFO-180 contamination on hydrocarbons removal were modelled using face-centred central composite design and ANOVA. Optimal washing parameters for sand contamination levels in the range 0.5-20 g/kg were identified with response surface methodology. While HPB-CD and SR performed equally to TX only at low sand contaminations, SL-1 attained hydrocarbon removal higher or equal to that of TX at any IFO-180 contamination and at lower application rates. SL-1 also outperformed TX when minimizing the water/sand ratio, i.e., the volume of water used. Considering its lower toxicity, higher biodegradability and higher hydrocarbon removal efficiencies, SL-1 is an effective and environmentally sustainable alternative to synthetic surfactants in washing treatments for marine fuel-contaminated sands

Varicella seroepidemiology and immunization in a cohort of future healthcare workers in the pre-vaccination era

Objectives: The goal of this study was to establish the seroprevalence of positive antibodies against varicella and compliance with varicella vaccination in the pre-vaccination era. Methods: A cohort of 10 683 Italian students from Padua University Medical School (from 2004 to 2019) were enrolled and classified as unvaccinated, vaccinated once, or vaccinated twice against varicella, according to their vaccination certificate. The antibody titre was measured and the seroprevalence of positive subjects was determined. Subjects with negative or equivocal antibodies were invited for vaccination, and then the antibody titre was retested. Results: Unvaccinated students were mostly seropositive (95.6%), compared with vaccinated students who were less seropositive (68.0% after one dose and 78.6% after two doses) and had significantly lower antibody titres (p < 0.0001). The post-test vaccination had a positive response rate of 85.4%: 67.4% after one dose and 91.4% after two doses. Conclusions: In the pre-vaccination era, only 3.3% of future healthcare workers were vaccinated against varicella (1.1% once and 2.2% twice). Vaccination or revaccination of negative and equivocal individuals could reduce the number of susceptible people. Implementation of varicella vaccine (two doses) in healthcare workers is of primary importance to reduce the risk of transmission

Start up of biological sequencing batch reactor (SBR) and short-term biomass acclimation for polyhydroxyalkanoates production

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