A computational method for the identification of dengue, zika and chikungunya virus species and genotypes

In recent years, an increasing number of outbreaks of Dengue, Chikungunya and Zika viruses have been reported in Asia and the Americas. Monitoring virus genotype diversity is crucial to understand the emergence and spread of outbreaks, both aspects that are vital to develop effective prevention and treatment strategies. Hence, we developed an efficient method to classify virus sequences with respect to their species and sub-species (i.e. serotype and/or genotype). This tool provides an easy-to-use software implementation of this new method and was validated on a large dataset assessing the classification performance with respect to whole-genome sequences and partial-genome sequences.publishersversionpublishe

Complete Genome Sequence of a Sucrose-Nonfermenting Epidemic Strain of Vibrio cholerae O1 from Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-11-29T16:27:51Z No. of bitstreams: 1 anaCP_vicente_etal_IOC_2012.pdf: 85091 bytes, checksum: 21a9ac0bb61adafc66c73f4f8f851d58 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-11-29T16:37:43Z (GMT) No. of bitstreams: 1 anaCP_vicente_etal_IOC_2012.pdf: 85091 bytes, checksum: 21a9ac0bb61adafc66c73f4f8f851d58 (MD5)Made available in DSpace on 2018-11-29T16:37:43Z (GMT). No. of bitstreams: 1 anaCP_vicente_etal_IOC_2012.pdf: 85091 bytes, checksum: 21a9ac0bb61adafc66c73f4f8f851d58 (MD5) Previous issue date: 2012Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Secção de Bacteriologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação Tecnológica. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil.Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.We report the genome sequence of Vibrio cholerae strain IEC224, which fails to ferment sucrose. It was isolated from a cholera outbreak in the Amazon. The defective sucrose phenotype was determined to be due to a frameshift mutation, and a molecular marker of the Latin American main epidemic lineage was identified

Characterization of three new insect-specific flaviviruses: Their relationship to the mosquito-borne flavivirus pathogens

Three novel insect-specific flaviviruses, isolated from mosquitoes collected in Peru, Malaysia (Sarawak), and the United States, are characterized. The new viruses, designated La Tina, Kampung Karu, and Long Pine Key, respectively, are antigenically and phylogenetically more similar to the mosquito-borne flavivirus pathogens, than to the classical insect-specific viruses like cell fusing agent and Culex flavivirus. The potential implications of this relationship and the possible uses of these and other arbovirus-related insect-specific flaviviruses are reviewed. Copyright © 2018 by The American Society of Tropical Medicine and Hygiene

A computational method for the identification of dengue, zika and chikungunya virus species and genotypes

In recent years, an increasing number of outbreaks of Dengue, Chikungunya and Zika viruses have been reported in Asia and the Americas. Monitoring virus genotype diversity is crucial to understand the emergence and spread of outbreaks, both aspects that are vital to develop effective prevention and treatment strategies. Hence, we developed an efficient method to classify virus sequences with respect to their species and sub-species (i.e. serotype and/or genotype). This tool provides an easy-to-use software implementation of this new method and was validated on a large dataset assessing the classification performance with respect to whole-genome sequences and partial-genome sequences. Available online: http://krisp.org.za/tools.php.SCOPUS: ar.jinfo:eu-repo/semantics/publishe