Resolution Study

Resolution effects on cartographic data using conventional stereoplotters with photographs taken at orbital height

Domains of invasion organelle proteins from apicomplexan parasites are homologous with the Apple domains of blood coagulation factor XI and plasma pre-kallikrein and are members of the PAN module superfamily

AbstractMicronemes are specialised organelles, found in all apicomplexan parasites, which secrete molecules that are essential for parasite attachment to and invasion of host cells. Regions of several microneme proteins have sequence similarity to the Apple domains (A-domains) of blood coagulation factor XI (FXI) and plasma pre-kallikrein (PK). We have used mass spectrometry on a recombinant-expressed, putative A-domain from the microneme protein EtMIC5 from Eimeria tenella, to demonstrate that three intramolecular disulphide bridges are formed. These bridges are analogous to those that stabilise A-domains in FXI and PK. The data confirm that the apicomplexan domains are structural homologues of A-domains and are therefore novel members of the PAN module superfamily, which also includes the N-terminal domains of members of the plasminogen/hepatocyte growth factor family. The role of A-domains/PAN modules in apicomplexan parasites is not known, but their presence in the microneme suggests that they may be important for mediating protein–protein or protein–carbohydrate interactions during parasite attachment and host cell invasion

Cost comparison of orthopaedic fracture pathways using discrete event simulation in a Glasgow hospital

Objective: Healthcare faces the continual challenge of improving outcome whilst aiming to reduce cost. The aim of this study was to determine the micro cost differences of the Glasgow non-operative trauma virtual pathway in comparison to a traditional pathway. Design:  Discrete event simulation was used to model and analyse cost and resource utilisation with an activity based costing approach. Data for a full comparison before the process change was unavailable so we utilised a modelling approach, comparing a Virtual Fracture Clinic (VFC) to a simulated Traditional Fracture Clinic (TFC). Setting:  The orthopaedic unit VFC pathway pioneered at Glasgow Royal Infirmary has attracted significant attention and interest and is the focus of this cost study. Outcome measures: Our study focused exclusively on non-operative trauma patients attending Emergency Department or the minor injuries unit and the subsequent step in the patient pathway. Retrospective studies of patient outcomes as a result of the protocol introductions for specific injuries in association with activity costs from the models.ResultsPatients are satisfied with the new pathway, the information provided and the outcome of their injuries (Evidence Level IV). There was a 65% reduction in the number of first outpatient face-to-face attendances in orthopaedics. In the VFC pathway, the resources required per day were significantly lower for all staff groups (p=<0.001). The overall cost per patient of the VFC pathway was £22.84 (95% CI: 21.74, 23.92) per patient compared with £36.81 (95% CI: 35.65, 37.97) for the TFC pathway.  Conclusions:  Our results give a clearer picture of the cost comparison of the virtual pathway over a wholly traditional face-to-face clinic system. The use of simulation-based stochastic costings in healthcare economic analysis has been limited to date, but this study provides evidence for adoption of this method as a basis for its application in other healthcare settings

Quantitative Spectroscopy of Supernovae for Dark Energy Studies

Detailed quantitative spectroscopy of Type Ia supernovae (SNe~Ia) provides crucial information needed to minimize systematic effects in both ongoing SNe Ia observational programs such as the Nearby Supernova Factory, ESSENCE, and the SuperNova Legacy Survey (SNLS) and in proposed JDEM missions such as SNAP, JEDI, and DESTINY. Quantitative spectroscopy is mandatory to quantify and understand the observational strategy of comparing ``like versus like''. It allows us to explore evolutionary effects, from variations in progenitor metallicity to variations in progenitor age, to variations in dust with cosmological epoch. It also allows us to interpret and quantify the effects of asphericity, as well as different amounts of mixing in the thermonuclear explosion.Comment: White paper submitted to the Dark Energy Task Force, 13 pages, 5 figure

Synthetic Spectra of Hydrodynamic Models of Type Ia Supernovae

We present detailed NLTE synthetic spectra of hydrodynamic SNe Ia models. We make no assumptions about the form of the spectrum at the inner boundary. We calculate both Chandrasekhar-mass deflagration models and sub-Chandrasekhar ``helium detonators.'' Gamma-ray deposition is handled in a simple, accurate manner. We have parameterized the storage of energy that arises from the time dependent deposition of radioactive decay energy in a reasonable manner, that spans the expected range. We find that the Chandrasekhar-mass deflagration model W7 of Nomoto etal shows good agreement with the observed spectra of SN 1992A and SN 1994D, particularly in the UV, where our models are expected to be most accurate. The sub-Chandrasekhar models do not reproduce the UV deficit observed in normal SNe Ia. They do bear some resemblance to sub-luminous SNe Ia, but the shape of the spectra (i.e. the colors) are opposite to that of the observed ones and the intermediate mass element lines such as Si II, and Ca II are extremely weak, which seems to be a generic difficulty of the models. Although the sub-Chandrasekhar models have a significant helium abundance (unlike Chandrasekhar-mass models), helium lines are not prominent in the spectra near maximum light and thus do not act as a spectral signature for the progenitor.Comment: submitted to ApJ, 26 pages, 10 figures, uses LaTeX styles aasms4.sty and natbib.sty Also available at: http://www.nhn.ou.edu/~baron

Gemini Photography Evaluation

Gemini photography evaluation for earth resources surve

In-vivo validity of proximal caries detection in primary teeth, with histological validation.

BACKGROUND: Detection and diagnosis of proximal caries in primary molars is challenging. AIM: The aim of this in-vivo study was to assess the validity and reproducibility of four methods of proximal caries detection in primary molar teeth. DESIGN: Eighty-two children (5-10 yrs) were recruited. Initially 1030 proximal surfaces were examined using meticulous visual examination (ICDAS) (VE1), bitewing radiographs (RE), and a laser fluorescence pen device (LF1). Temporary tooth separation (TTS) was achieved for 447 surfaces and these were re-examined visually (VE2) and using the LF-pen (LF2). Three hundred and fifty-six teeth (542 surfaces) were subsequently extracted and provided histological validation. RESULTS: At D1 (enamel and dentine caries) diagnostic threshold, the sensitivity of VE1, RE, VE2, LF1 and LF2 examination were 0.52, 0.14, 0.75, 0.58, 0.60 and the specificity values were 0.89, 0.97, 0.88, 0.85, 0.77 respectively. At D3 (dentine caries) threshold, the sensitivity values were 0.42, 0.71, 0.49, 0.63, 0.65 respectively, while specificity was 0.93 for VE1 and VE2, and 0.98, 0.87 and 0.88 for RE, LF1 and LF2 examinations respectively. ROC analysis showed radiographic examination to be superior at D3 . CONCLUSION: Meticulous caries diagnosis (ICDAS) should be supported by radiographs for detection of dentinal proximal caries in primary molars