Massive Protoplanetary Disks in the Trapezium Region

(abridged) We determine the disk mass distribution around 336 stars in the young Orion Nebula cluster by imaging a 2.5' x 2.5' region in 3 mm continuum emission with the Owens Valley Millimeter Array. For this sample of 336 stars, we observe 3 mm emission above the 3-sigma noise level toward ten sources, six of which have also been detected optically in silhouette against the bright nebular background. In addition, we detect 20 objects that do not correspond to known near-IR cluster members. Comparisons of our measured fluxes with longer wavelength observations enable rough separation of dust emission from thermal free-free emission, and we find substantial dust emission toward most objects. For the ten objects detected at both 3 mm and near-IR wavelengths, eight exhibit substantial dust emission. Excluding the high-mass stars and assuming a gas-to-dust ratio of 100, we estimate circumstellar masses ranging from 0.13 to 0.39 Msun. For the cluster members not detected at 3 mm, images of individual objects are stacked to constrain the mean 3 mm flux of the ensemble. The average flux is detected at the 3-sigma confidence level, and implies an average disk mass of 0.005 Msun, comparable to the minimum mass solar nebula. The percentage of stars in Orion surrounded by disks more massive than ~0.1 Msun is consistent with the disk mass distribution in Taurus, and we argue that massive disks in Orion do not appear to be truncated through close encounters with high-mass stars. Comparison of the average disk mass and number of massive dusty structures in Orion with similar surveys of the NGC 2024 and IC 348 clusters constrains the evolutionary timescales of massive circumstellar disks in clustered environments.Comment: 27 pages, including 7 figures. Accepted by Ap

A brown dwarf companion to the intermediate-mass star HR6037

In the course of an imaging survey we have detected a visual companion to the intermediate-mass star HR 6037. In this letter, we present two epoch observations of the binary with NACO/VLT, and near-IR spectroscopy of the secondary with ISAAC/VLT. The NACO observations allow us to confirm HR 6037B as a co-moving companion. Its J and H band ISAAC spectra suggest the object has an spectral type of M9+-1, with a surface gravity intermediate between that of 10 Myr dwarfs and field dwarfs with identical spectral type. The comparison of its Ks-band photometry with evolutionary tracks allows us to derive a mass, effective temperature, and surface gravity of 62+-20 MJup, Teff = 2330+-200 K, and log g = 5.1+-0.2, respectively. The small mass ratio of the binary, -0.03, and its long orbital period, -5000 yr, makes HR 6037 a rare and uncommon binary system.Comment: (5 pages, 4 figures, accepted for publication in A&A Letters

Proplyds and Massive Disks in the Orion Nebula Cluster Imaged with CARMA and SMA

[Abridged] We imaged a 2' x 2' region of the Orion Nebula cluster in 1.3 mm wavelength continuum emission with the recently commissioned Combined Array for Research in Millimeter Astronomy (CARMA) and with the Submillimeter Array (SMA). Our mosaics include >250 known near-IR cluster members, of which 36 are so-called "proplyds" that have been imaged previously with the Hubble Space Telescope. We detected 40 sources in 1 mm continuum emission, and several of them are spatially resolved with our observations. Dust masses inferred for detected sources range from 0.01 to 0.5 Msun, and the average disk mass for undetected sources is estimated to be ~0.001 Msun, approximately an order of magnitude smaller than the minimum mass solar nebula. Most stars in the ONC thus do not appear to currently possess sufficient mass in small dust grains to form Jupiter-mass (or larger) planets. Comparison with previous results for younger and older regions indicates that massive disks evolve significantly on ~Myr timescales. We also show that the percentage of stars in Orion surrounded by disks more massive than ~0.01 Msun is substantially lower than in Taurus, indicating that environment has an impact on the disk mass distribution. Finally, we explore potential correlations of disk mass with stellar mass and location within the cluster.Comment: 45 pages, 11 figures. Accepted for publication in Ap

Thermodynamic Driving Forces of Guest Confinement in a Photoswitchable Cage

Photoswitchable cages that confine small guest molecules inside their cavities offer a way to control the binding/unbinding process through irradiation with light of different wavelengths. However, a detailed characterization of the structural and thermodynamic consequences of photoswitching is very challenging to obtain by experiment alone. Thus, all-atom molecular dynamics (MD) simulations were carried out to gain insight into the relationship between structure and binding affinity. Binding free energies of the B12F122- guest were obtained for all photochemically accessible forms of a photoswitchable dithienylethene (DTE) based coordination cage. The MD simulations show that successive photo-induced closure of the four individual DTE ligands that form the cage gradually decreases the binding affinity. Closure of the first ligand already significantly lowers the unbinding barrier and the binding free energy, and therefore favours guest unbinding both kinetically and thermodynamically. Analysis of the different enthalpy contributions to the free energy shows that binding is enthalpically unfavourable and thus an entropy-driven process, in agreement with experimental data. Dissecting the enthalpy into the contributions from electrostatic, van der Waals, and bonded interactions in the force field shows that the unfavourable binding enthalpy is due to the bonded interactions being more favourable in the dissociated state, suggesting the presence of structural strain in the bound complex. Thus, the simulations provide microscopic explanations for the experimental findings and open a possible route towards the targeted design of switchable nanocontainers with modified binding properties

Steering the Ultrafast Opening and Closure Dynamics of a Photochromic Coordination Cage by Guest Molecules

Photochemical studies on supramolecular hosts that can encapsulate small guest molecules commonly focus on three aspects: photoswitching the cage to release or trap the guest, the effect of the confining environment on the guest, and light-induced exciton or charge transfer within the cage structure. Here, we exploit ultrafast spectroscopy to address how the guest alters the photoswitching characteristics of the cage. For this, the impacts of three disparate guest compounds on ring-opening or ring-closure of a dithienylethene (DTE) ligand in a photoswitchable DTE-based coordination cage are juxtaposed. The guest modulates both outcome and timescale of the cage's photodynamics, by an interplay of structural strain, heavy-atom effect, and enhancement of charge-transfer processes exercised by the guest on the photo-excited cage. The approach might prove beneficial for attuning the applicability of photoswitchable nanocontainers and desired guest compounds

Steuerung der ultraschnellen Öffnungs‐ und Schließungsdynamik eines photochromen Koordinationskäfigs durch Gastmoleküle

Photochemische Studien über supramolekulare Wirte, die kleine Gastmoleküle einkapseln können, konzentrieren sich zumeist auf drei Aspekte: Die Photoschaltung des Käfigs, um den Gast freizusetzen oder einzufangen, die Wirkung der Käfigumgebung auf den Gast und die lichtinduzierte Exzitonen- oder Ladungsübertragung innerhalb der Käfigstruktur. Hier nutzen wir ultraschnelle Spektroskopie, um zu untersuchen, wie der Gast die Photoschaltcharakteristik des Käfigs verändert. Zu diesem Zweck werden die Auswirkungen von drei unterschiedlichen Gastmolekülen auf die Ringöffnung oder den Ringschluss eines Dithienylethen (DTE)-Liganden in einem photoschaltbaren Koordinationskäfig auf DTE-Basis einander gegenübergestellt. Der Gast moduliert sowohl das Ergebnis als auch die Zeitskala der Photodynamik des Käfigs durch ein Zusammenspiel von struktureller Wechselwirkung, dem Schweratomeffekt und einer Verstärkung von Ladungstransferprozessen, die der Gast auf den photoangeregten Käfig ausübt. Der Ansatz könnte sich als nützlich erweisen, um die Anwendbarkeit von photoschaltbaren Nanocontainern und gewünschten Gastverbindungen aufeinander abzustimmen

An oomycete NLP cytolysin forms transient small pores in lipid membranes

Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1-like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.Peer reviewe

Mix&Match: Towards Omitting Modelling Through In-Situ Alteration and Remixing of Model Repository Artifacts in Mixed Reality

The accessibility of tools to model artifacts is one of the core driving factors for the adoption of Personal Fabrication. Subsequently, model repositories like Thingiverse became important tools in (novice) makers' processes. They allow them to shorten or even omit the design process, offloading a majority of the effort to other parties. However, steps like measurement of surrounding constraints (e.g., clearance) which exist only inside the users' environment, can not be similarly outsourced. We propose Mix&Match a mixed-reality-based system which allows users to browse model repositories, preview the models in-situ, and adapt them to their environment in a simple and immediate fashion. Mix&Match aims to provide users with CSG operations which can be based on both virtual and real geometry. We present interaction patterns and scenarios for Mix&Match, arguing for the combination of mixed reality and model repositories. This enables almost modelling-free personal fabrication for both novices and expert makers.Comment: 12 pages, 15 figures, 1 table, To appear in the Proceedings of the ACM Conference on Human Factors in Computing Systems 2020 (CHI'20

Combinatorial scaffold morphologies for zonal articular cartilage engineering

AbstractArticular cartilage lesions are a particular challenge for regenerative medicine strategies as cartilage function stems from a complex depth-dependent organization. Tissue engineering scaffolds that vary in morphology and function offer a template for zone-specific cartilage extracellular matrix (ECM) production and mechanical properties. We fabricated multi-zone cartilage scaffolds by the electrostatic deposition of polymer microfibres onto particulate-templated scaffolds produced with 0.03 or 1.0mm3 porogens. The scaffolds allowed ample space for chondrocyte ECM production within the bulk while also mimicking the structural organization and functional interface of cartilage’s superficial zone. Addition of aligned fibre membranes enhanced the mechanical and surface properties of particulate-templated scaffolds. Zonal analysis of scaffolds demonstrated region-specific variations in chondrocyte number, sulfated GAG-rich ECM, and chondrocytic gene expression. Specifically, smaller porogens (0.03mm3) yielded significantly higher sGAG accumulation and aggrecan gene expression. Our results demonstrate that bilayered scaffolds mimic some key structural characteristics of native cartilage, support in vitro cartilage formation, and have superior features to homogeneous particulate-templated scaffolds. We propose that these scaffolds offer promise for regenerative medicine strategies to repair articular cartilage lesions