Estudo de tipos sanguineos em bovinos selecionados para leite e para corte da raça Gir (Bos indiais) criada no Brasil

Orientador : Bernardo BeiguelmanTese (doutorado) - Universidade Estadual de Campinas, Instituto de BiologiaResumo: o objetivo do presente trabalho foi caracterizar, por meio de marcadores geneticos do sangue, a raça zebuina Gir criada no Brasil, avaliando as dlferenças entre as variedades leiteira e de corte. Foram feitos testes serológicoa para trinta e tres especificidades antigenicas, referentes a nove s1stemas de grupos sangdlneos (A, B, C, F, J, L, M, S e Z) e testes eletroforeticos em gel de amido, para quatro sistemas de polimorfismos bioquimicos (albumlna, anldrase carbonica, hemogloblna e transferrlna). A amostra consistiu de 922 animais, sendo 476 do grupo de lelte e 446 do grupo de corte, além de 358 descendentes lncluídos na anàlise de determlnaçao dos fenogrupos do sistema B. ... Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digitalAbstract: The purpose of this study was to characterize by means of blood genetec markers, the Gyr breed raised in Brazil, ai well as to evaluate the differences between dairy and beef herds within this breed. Serological tests for 33 specificities of 9 blood group aystems (A, B, C, F, J, L, S, K and Z) and starch gel electrophoresis for albumin, carbonic anhydraae, hemoglobin and transferrin systems were done. A total of 922 animals were analysed, and of those 476 were classified as dairy cattle and 446 as beef cattle. In addition to that, 358 offsprings were typed for the purpose of phenogroup determination in the B system. ... Note: The complete abstract is available with the full electronic digital thesis or dissertationsDoutoradoGeneticaDoutor em Ciências Biológica

Genetic variability of 10 microsatellite markers in the characterization of Brazilian Nellore cattle (Bos indicus)

We assessed the polymorphism of 10 microsatellites in Brazilian Nellore cattle (Bos indicus) using a commercial multiplex system. Allele frequencies, polymorphism information content, heterozygosity and exclusion probability were calculated. Allele frequencies revealed that in the sample analyzed the markers were not equally polymorphic. The exclusion probabilities and the polymorphism information content of some loci in Nellore cattle were lower than in Bos taurus breeds. When all the microsatellites were considered the combined exclusion probability was 0.9989. This multiplex analysis can contribute toward pedigree information, adequate genetic improvements and breeding programs

Data from: Conservation genetics of Neotropical pollinators revisited: microsatellite analysis suggests that diploid males are rare in orchid bees

Allozyme analyses have suggested that Neotropical orchid bee (Euglossini) pollinators are vulnerable because of putative high frequencies of diploid males, a result of loss of sex allele diversity in small hymenopteran populations with single locus complementary sex determination. Our analysis of 1010 males from 27 species of euglossine bees sampled across the Neotropics at 2-11 polymorphic microsatellite loci revealed only 5 diploid males at an overall frequency of 0.005 (95% CIs 0.002-0.010); errors through genetic non-detection of diploid males were likely small. In contrast to allozyme-based studies, we detected very weak or insignificant population genetic structure, even for a pair of populations >500 km apart, possibly accounting for low diploid male frequencies. Technical flaws in previous allozyme-based analyses have probably led to considerable overestimation of diploid male production in orchid bees. Other factors may have a more immediate impact on population persistence than the genetic load imposed by diploid males on these important Neotropical pollinators

Haplotypes/genotypes of individuals at microsatellite loci

Haplotypes (for diploid males: genotypes) of all males collected in the field and analyzed at several microsatellite loci, as presented in the original Evolution paper