Characterization antibacterial constituent from Ficus deltoideusJack leaves

An antibacterial constituent, has been isolated from Ficus deltoideus Jack leaves. Based on spectroscopic data (IR, 1H-NMR, 13 C NMR 1D and 2D and MS), the structure of this compound was identified as Olean-12en-3β-ol, (β-amyrin), C30H50O. This compound showed antibacterial activities against E. coli, B. subtilis and S.  aureus.  The minimum inhibition concentration (MIC) agains  E.  coli,  B. subtilisand  S. aureusare 230, 380 and 460 (µg/mL) respectively.Key words: Antibacterial activity,Ficus deltoideusJack, β-amyri

Characterization antibacterial constituent from Ficus deltoideus Jack leaves Karakterisasi konstituen antibakteri dari daun Ficus deltoideus Jack

An antibacterial constituent, has been isolated from Ficus deltoideus Jack leaves. Based on spectroscopic data (IR, lH-NMR, 13CNMRlD and 2D and MS), the structure of this compound was identified as Olean-12en-3&#946-ol, (&#946-amyrln), C&#8323&#8320H&#8325&#8320O. This compound showed antibacterial activities against E. coli, B. subtilis and S. aureus. The minimum inhibition concentration (MIC) agains E. coli, B. subtilis and S. aureus are 230, 380 and 460 (µ/mL) respectively. Suatu konstituen antibakteri, telah diisolasi dari daun Ficus deltoideus Jack. Karakterisasi struktur menggunakan data spektroskopi IR, lH-NMR, 13C_ NMR, lD dan 2D serta data MS, menunjukkan bahwa konstituen hasll isolasi adalah Olean-12en-3&#946-0I, (&#946-amyrin), C&#8323&#8320H&#8325&#8320O. Uji aktivitas antibakteri terhadap E. coli, B.subtilis dan S. aureus menunjukkan bahwa &#946-amyrin dapat menghambat pertumbuhan bakteri, dengan nllai MIC 230 µ/mL untuk E. coli, 380 µ/mLuntuk B. subtilis, dan 460 µ/mL untuk bakteri S. aureus

STRUCTURE ELUCIDATION OF ANTIBACTERIAL COMPOUND FROM <i>Ficus deltoidea</i> Jack LEAVES

An antibacterial compound has been isolated from Ficus deltoidea Jack leaves. Based on spectroscopic data (IR, 1H-NMR, 13C NMR 1D and 2D and MS), the structure of this compound was identified as 3β-hydroksilup-20(29)-en, (lupeol), C30H50O. This compound showed antibacterial activities against E. coli, B. subtilis and S. aureus. The minimum inhibition concentration (MIC) against E. coli, B. subtilis and S. aureus are 150, 220 and 130 μg/mL respectively

PROFIL FITOKIMIA DAN AKTIFITAS ANTIACETYLCHOLINESTERASE DARI DAUN TABAT BARITO (Ficus Deltoidea Jack)

Phytochemical study and antiacetylcholinesterase assay by using bioautography method were carried out on polar fraction of tabat barito (Ficus deltoidea jack) leaves. The result of this studies showed that the leaves contained flavonoid, terpenoid, steroid and fenolic compounds. This extract gave positive result on antiacetylcholinesterase. Keywords: Antiacetylcholinesterase, Ficus deltoidea Jac

Cytotoxic Aaptamines from Malaysian Aaptos aaptos

In a preliminary screen, Aaptos aaptos showed significant cytotoxic activity towards a panel of cell lines and was thus subjected to bioassay-guided isolation of the bioactive constituents. In addition to the known aaptamine, two new derivatives of the alkaloid were isolated from the bioactive chloroform fraction of the crude methanolic extract. Detailed analysis by NMR and mass spectroscopy enabled their identification to be 3-(phenethylamino)demethyl(oxy)aaptamine and 3-(isopentylamino)demethyl(oxy) aaptamine. The cytotoxic activities of the three alkaloids were further evaluated against CEM-SS cells

Vasorelaxant activity of indole alkaloids from Tabernaemontana dichotoma.

The aim of this study was to search for bioactive natural products from medicinal plants targeting vasorelaxant activity and we found the methanol extract from bark of Tabernaemontana dichotoma showed vasorelaxant activity on rat aorta. We isolated eight indole alkaloids including 10-methoxyalstonerine (1), a new macroline type indole alkaloid, from bark of T. dichotoma. These were respectively identified as 10-methoxyaffinisine (2), lochnerine (3), cathafoline (4), (−)-alstonerine (5), 19,20-dehydro-10-methoxytalcarpine (6), alstonisine (7), and alstonal (8) based on spectroscopic analysis. Among them, sarpagine type (2 and 3), akuammiline type (4), and macroline oxindole type (7 and 8) showed potent vasorelaxant activity. Mechanism of action on vasorelaxant activity of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) was clarified. Effects of 10-methoxyaffinisine (2), cathafoline (4), and alstonisine (7) were partially mediated the NO release from endothelial cells. Furthermore, 10-methoxyaffinisine (2) and alstonisine (7) attribute to the inhibitory effect of VDC and ROC, and cathafoline (4) have inhibitory effect on Ca2+ influx via ROC. In addition, 10-methoxyaffinisine (2) as a major compound from bark of T. dichotoma showed hypotensive effect on normotensive rats in vivo

CYTOTOXICITY STUDIES OF TETRAPRELYLTOLUQUINONE, A PRENILATED HYDROQUINONE FROM GARCINA COWA ROXB ON H-460, MCF-7 AND DU-145

Objective: The aim of the present study was to examine the cytotoxicity of a new ring-reduced tetra prenyltoluquinone (TPTQ), [2E,6E,10E]-(+)-4b-hydroxy-3-methyl-5b-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenyl-2-cyclohexen-1-one against H-460, MCF-7 and DU-145 cell lines.Methods: Different concentrations of TPTQ were subjected to cytotoxicity study by using MTT method and calculate the percentage of cell viability.Results: The results of this study showed that this compound has IC50 value 16.3 ± 3.0 µM in H-460 cancer cell lines without any activities towards another two type of cell lines.Conclusion: TPTQ had selective activity against H-460 cancer cell lines.Â

Inhabitation Effect of Linoleic Acid, the Ingredient of Nigella sativa (Black Seed) on MDA-MB-231 and MCF-7 Human Breast Cancer Cells

Objective: The objective of this study was to investigate inhibition and anti-cancer effects of Linoleic acid on the MCF-7 and MDA-MB-231 human breast cancer cells. Materials and methods: Cell lines Human breast cancer MCF-7 (GDC055) and MDA-MB-231 (HTB-26) cell lines were obtained from ATCC. MCF-7 estrogen receptor positive human breast cancer cell line and  the estrogen receptor negative human breast cancer cell line MDA-MB-231, were grown in DMEM. MDA-MB-231 and MCF-7 human breast cancer cell lines were observed. For each experiment, seven doses were considered diluting from the highest to the lowest doses by half, respectively. MTS apoptosis  and cytotoxic activity assay were used in order to find toxic effects, and the results were supported by flow cytometry (Cell cycle analysis). Results: The results showed the cytotoxic effect of Linoleic acid on the breast cancer cell lines that can be posed as an anti-cancer effect of lionleic acid. According to our findings, when the concentration of lionleic acid was increased, compared with the concentrations currently being reported, it shows anti-cancer effects. Conclusion: It was concluded that Linoleic acid has an inhibiting effect on human breast cancer cell lines which can be due to its two double-bandings molecular structure

Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects