Characterization of biocompatible scaffolds manufactured by fused filament fabrication of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate

We characterize poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) scaffolds for tissue repair and regeneration, manufactured by three-dimensional fused filament fabrication (FFF). PHBH belongs to the class of polyhydroxyalkanoates with interesting biodegradable and biocompatible capabilities, especially attractive for tissue engineering. Equally, FFF stands as a promising manufacturing technology for the production of custom-designed scaffolds. We address thermal, rheological and cytotoxicity properties of PHBH, placing special emphasis on the mechanical response of the printed material in a wide deformation range. Indeed, effective mechanical properties are assessed in both the linear and nonlinear regime. To warrant uniqueness of the material parameters, these are measured directly through digital image correlation, both in tension and compression, while experimental data fitting of finite-element analyses is only adopted for the determination of the second invariant coefficient in the nonlinear regime. Mechanical data are clearly porosity dependent, and they are given for both the cubic and the honeycomb infill pattern. Local strain spikes due to the presence of defects are observed and measured: those falling in the range 70\u2013100% lead to macro-crack development and, ultimately, to failure. Results suggest the significant potential attached to FFF printing of PHBH for customizable medical devices which are biocompatible and mechanically resilient

Bifidobacteria and lactobacilli in the gut microbiome of children with non-alcoholic fatty liver disease: which strains act as health players?

Introduction: Non-alcoholic fatty liver disease (NAFLD), considered the leading cause of chronic liver disease in children, can often progress from non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH). It is clear that obesity is one of the main risk factors involved in NAFLD pathogenesis, even if specific mechanisms have yet to be elucidated. We investigated the distribution of intestinal bifidobacteria and lactobacilli in the stools of four groups of children: obese, obese with NAFL, obese with NASH, and healthy, age-matched controls (CTRLs). Material and methods: Sixty-one obese, NAFL and NASH children and 54 CTRLs were enrolled in the study. Anthropometric and metabolic parameters were measured for all subjects. All children with suspected NASH underwent liver biopsy. Bifidobacteria and lactobacilli were analysed in children’s faecal samples, during a broader, 16S rRNA-based pyrosequencing analysis of the gut microbiome. Results: Three Bifidobacterium spp. (Bifidobacterium longum, Bifidobacterium bifidum, and Bifidobacterium adolescentis) and five Lactobacillus spp. (L. zeae, L. vaginalis, L. brevis, L. ruminis, and L. mucosae) frequently recurred in metagenomic analyses. Lactobacillus spp. increased in NAFL, NASH, or obese children compared to CTRLs. Particularly, L. mucosae was significantly higher in obese (p = 0.02426), NAFLD (p = 0.01313) and NASH (p = 0.01079) than in CTRLs. In contrast, Bifidobacterium spp. were more abundant in CTRLs, suggesting a protective and beneficial role of these microorganisms against the aforementioned diseases. Conclusions: Bifidobacteria seem to have a protective role against the development of NAFLD and obesity, highlighting their possible use in developing novel, targeted and effective probiotics

Application of key performance indicators for maintenance management of clinics facilities

The investment in the development of the existing portfolio of clinics, as well as in their maintenance and operation, constitutes 3–4% of the annual budgets of health maintenance organizations (HMOs) in Israel. These clinics are the primary facilities for the provision of health services. The research objective was the implementation of previously defined key performance indicators (KPIs) in a 42 clinic sample in order to: (1) assess the maintenance performance of the facilities, (2) intraand inter-benchmark the performance and efficiency, (3) establish a policy for the strategic and tactical maintenance management, and (4) set priorities in the maintenance plan. These actions should be part of a cycle for continuous improvement of the performance and maintenance of the facilities. This set of seven hybrid KPIs can be used for the leading and lagging analysis of the planning and control of facility maintenance. A case study is presented as an example of the applicability of the method

Otoacoustic Emissions Simulated in the Time-Domain by a Hydroynamic Model of the Human Cochlea

Time-domain simulations of the response to click of a human ear show that, if the cochlear amplifier gain (CAG) is a smooth function of basilar-membrane (BM) position, the filtering performed by a middle ear with an irregular (non-smooth) transfer function suffices to produce irregular and long-lasting residual BM oscillations at selected frequencies. Feeding back to the middle ear through hydrodynamic coupling, these oscillations are detected as otoacoustic emissions (OAEs) in the ear canal. If, in addition, also the CAG profile is irregular, residual BM oscillations are even more irregular, often ensuing to self-sustaining oscillations at CAG irregularity loci. Correspondingly, transient evoked OAE spectra exhibit sharp peaks. If both the CAG and the middle-ear transfer function are smooth, residual BM oscillations are characterized by regular waveform, extinguish rapidly and do not generate appreciable emission. Simulating localized damage to the cochlear amplifier results in spontaneous emissions and stimulus-frequency OAEs, with typical modulation patterns, for inputs near hearing threshold

Constraining the relative inclinations of the planets B and C of the millisecond pulsar PSR B1257+12

We investigate on the relative inclination of the planets B and C orbiting the pulsar PSR B1257+12 in connection with potential violations of the equivalence principle (Abridged).Comment: LaTex2e, 10 pages, 1 table, 3 figures, 17 references. Small stylistic changes. Version to appear in Journal of Astrophysics and Astronomy (JAA

On the possibility of measuring relativistic gravitational effects with a LAGEOS-LAGEOS II-OPTIS-mission

In this paper we wish to preliminary investigate if it would be possible to use the orbital data from the proposed OPTIS mission together with those from the existing geodetic passive SLR LAGEOS and LAGEOS II satellites in order to perform precise measurements of some general relativistic gravitoelectromagnetic effects, with particular emphasis on the Lense-Thirring effect.Comment: Abridged version. 16 pages, no figures, 1 table. First results from the GGM01C Earth gravity model. GRACE data include

Cardiac autonomic control in Rett syndrome: Insights from heart rate variability analysis

Rett syndrome (RTT) is a rare and severe neurological disorder mainly affecting females, usually linked to methyl-CpG-binding protein 2 (MECP2) gene mutations. Manifestations of RTT typically include loss of purposeful hand skills, gait and motor abnormalities, loss of spoken language, stereotypic hand movements, epilepsy, and autonomic dysfunction. Patients with RTT have a higher incidence of sudden death than the general population. Literature data indicate an uncoupling between measures of breathing and heart rate control that could offer insight into the mechanisms that lead to greater vulnerability to sudden death. Understanding the neural mechanisms of autonomic dysfunction and its correlation with sudden death is essential for patient care. Experimental evidence for increased sympathetic or reduced vagal modulation to the heart has spurred efforts to develop quantitative markers of cardiac autonomic profile. Heart rate variability (HRV) has emerged as a valuable non-invasive test to estimate the modulation of sympathetic and parasympathetic branches of the autonomic nervous system (ANS) to the heart. This review aims to provide an overview of the current knowledge on autonomic dysfunction and, in particular, to assess whether HRV parameters can help unravel patterns of cardiac autonomic dysregulation in patients with RTT. Literature data show reduced global HRV (total spectral power and R-R mean) and a shifted sympatho-vagal balance toward sympathetic predominance and vagal withdrawal in patients with RTT compared to controls. In addition, correlations between HRV and genotype and phenotype features or neurochemical changes were investigated. The data reported in this review suggest an important impairment in sympatho-vagal balance, supporting possible future research scenarios, targeting ANS

"Galileo Galilei" (GG) a small satellite to test the equivalence principle of Galileo, Newton and Einstein

"Galileo Galilei" (GG) is a small satellite designed to fly in low Earth orbit with the goal of testing the Equivalence Principle-which is at the basis of the General Theory of Relativity-to 1 part in 1017. If successful, it would improve current laboratory results by 4 orders of magnitude. A confirmation would strongly constrain theories; proof of violation is believed to lead to a scientific revolution. The experiment design allows it to be carried out at ambient temperature inside a small 1-axis stabilized satellite (250 kg total mass). GG is under investigation at Phase A-2 level by ASI (Agenzia Spaziale Italiana) at Thales Alenia Space in Torino, while a laboratory prototype (known as GGG) is operational at INFN laboratories in Pisa, supported by INFN (Istituto Nazionale di fisica Nucleare) and ASI. A final study report will be published in 2009

Perspectives on the diagnosis and management of functional cognitive disorder: An international Delphi study

BACKGROUND: Current proposed criteria for functional cognitive disorder (FCD) have not been externally validated. We sought to analyse the current perspectives of cognitive specialists in the diagnosis and management of FCD in comparison with neurodegenerative conditions. METHODS: International experts in cognitive disorders were invited to assess seven illustrative clinical vignettes containing history and bedside characteristics alone. Participants assigned a probable diagnosis and selected the appropriate investigation and treatment. Qualitative, quantitative and inter-rater agreement analyses were undertaken. RESULTS: Eighteen diagnostic terminologies were assigned by 45 cognitive experts from 12 countries with a median of 13 years of experience, across the seven scenarios. Accurate discrimination between FCD and neurodegeneration was observed, independently of background and years of experience: 100% of the neurodegenerative vignettes were correctly classified and 75%-88% of the FCD diagnoses were attributed to non-neurodegenerative causes. There was <50% agreement in the terminology used for FCD, in comparison with 87%-92% agreement for neurodegenerative syndromes. Blood tests and neuropsychological evaluation were the leading diagnostic modalities for FCD. Diagnostic communication, psychotherapy and psychiatry referral were the main suggested management strategies in FCD. CONCLUSIONS: Our study demonstrates the feasibility of distinguishing between FCD and neurodegeneration based on relevant patient characteristics and history details. These characteristics need further validation and operationalisation. Heterogeneous labelling and framing pose clinical and research challenges reflecting a lack of agreement in the field. Careful consideration of FCD diagnosis is advised, particularly in the presence of comorbidities. This study informs future research on diagnostic tools and evidence-based interventions