Produção e caracterização do biossurfatante de Bacillus subtilis utilizando manipueira como substrato

Orientador: Glaucia Maria PastoreTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de AlimentosDoutorad

Acolhimento temporário institucional

RESUMO Este trabalho tem como objectivo principal um estudo de campo no Centro de Acolhimento Temporário Gracinda Tito com uma população de 18 crianças e jovens institucionalizados da RAM. Pretende apresentar a temática teórica e legal do acolhimento temporário, caracterizar a instituição-alvo e os participantes no estudo e, finalmente, tentar saber a razão pelo qual as crianças/jovens foram acolhidas, bem como a medida de promoção e protecção aplicada a cada criança e jovem e o seu projecto de vida futuro.ABSTRACT The main objective of this research work about temporary institutional reception, aims to study the eighteen children and young people received at Gracinda Tito Temporary Reception Center. This research comes to know the reason why children and young people were received, as well as the promotion and protection measure applied to every child and young, and their future life project

Biosurfactants: properties and applications

Chemically synthesized surfactants are widely used for many purposes in almost every sector of modern industry. Surface-active compounds of biological origin (biosurfactants) have been gaining attention in recent years because of some advantages such as biodegradability, low toxicity, diversity of applications and functionality under extreme conditions. Microbial biosurfactants are useful in bioremediation of water and soil, enhanced oil recovery, and in many formulations of petrochemical, chemical, pharmaceutical, food, cosmetic and textile industries. The importance of biosurfactants, their characteristics and industrial applications are discussed.77277

Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces

Bacterial adhesion to inert surfaces is a complex process influenced by environmental conditions. In this work, the influence of growth medium and temperature on the adhesion of Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes to polystyrene surfaces was studied. Most bacteria demonstrated the highest adhesion when cultured in TSYEA, except S. marcescens, which showed to be positively influenced by the pigment production, favored in poor nutrient media (lactose and peptone agar). P. aeruginosa adhesion to polystyrene increased at low temperatures whatever the medium used. The culture medium influenced the surface properties of the bacteria as assessed by the MATS test

Hurdle Technology Approach to Control Listeria monocytogenes Using Rhamnolipid Biosurfactant

This study evaluates the combination of mild heat with a natural surfactant for the inactivation of L. monocytogenes Scott A in low-water-activity (aw) model systems. Glycerol or NaCl was used to reduce the aw to 0.92, and different concentrations of rhamnolipid (RL) biosurfactant were added before heat treatment (60 °C, 5 min). Using glycerol, RL treatment (50–250 µg/mL) reduced bacterial population by less than 0.2 log and heat treatment up to 1.5 log, while the combination of both hurdles reached around 5.0 log reduction. In the NaCl medium, RL treatment displayed higher inactivation than in the glycerol medium at the same aw level and a larger synergistic lethal effect when combined with heat, achieving ≥ 6.0 log reduction at 10–250 µg/mL RL concentrations. The growth inhibition activity of RL was enhanced by the presence of the monovalent salts NaCl and KCl, reducing MIC values from >2500 µg/mL (without salt) to 39 µg/mL (with 7.5% salt). The enhanced antimicrobial activity of RL promoted by the presence of salts was shown to be pH-dependent and more effective under neutral conditions. Overall, results demonstrate that RL can be exploited to design novel strategies based on hurdle approaches aiming to control L. monocytogenes

Cassava Flour Wastewater As A Substrate For Biosurfactant Production.

Five cassava flour wastewater (manipueira) preparations were tested as culture media for biosurfactant production by a wild-type Bacillus sp. isolate. No-solids (F), no-solids diluted (F/2), natural (I), natural diluted (I/2), and decanted (IPS) were the tested manipueira media. The microorganism was able to grow and to produce biosurfactant on all manipueira preparations. The media whose solids were removed (F and F/2) showed better results than preparations with the presence of solids (I, I/2, and IPS). No-solids medium (F) showed a surface tension of 26,59 mN/m and reciprocal of critical micelle concentration of over 100 and was selected as a potential substrate for biosurfactant production.105 -108295-30