Glibenklamidin sulfonylurea glyburide on catalase activities in streptozotocin-ınduced diabetic rat muscle

Purpose; Free radicals, those are chemical species possessing an unpaired electron in their molecular or atomic orbit, have been the most attractive subjects in medicine because of their roles in destruction of cell or tissue. Many findings support the view that free radicals and oxidative stress play an important role in etiology of diabetes and its complications. Materials and methods; Hence, in the present investigation, we administrated glyburide to streptozotosin-induced diabetic rats and determined the affect of glyburide on muscle (M. gastrocinemius, M. soleus, M. quadriceps femoris) catalase activities. Rats (Sprague Dawley), weighing 150- 200g were used in the present investigation. The experimental group was injected intraperitonally with streptozotosin (STZ, freshly dissolved in citrate buffer, pH 4.5 55 mg\kg) The proceeded data had been provided out from statisticaly (SPSS 10,0) based ware. Results; In the present study, muscle CAT activty was significantly reduced(p<0.001) in STZ-induced diabetic rats. Effecting glyburide treatment on diabetic rat muscles has been seen to make a measurable improvement creating a slight reduction on the decrease rate; however the above mentioned affect has not been traced on gastrocinemius muscles. Conclusion; This study could not cover an intention to find out the mechanism of restrotation of glyburide‘s decreasing affect level on catalayse. Further studies are needed to provide positive adds both for viewing the levels and mechanism of glyburide restration and expressing the the pathogenesis of type II diabetes.Moleküler veya atomik yörüngelerinde çiftleşmemiş e- bulunduran moleküller olan serbest radikaller; dokularda, hücre hasarı oluşumundaki rolleri ile, son yıllarda, tıbbın en ilgi çekici konularından biri durumuna gelmiştir. Diabet ve komplikasyonlarının oluşumunda, serbest radikaller ve oksidatif stresin rol oynayabileceğine ilişkin birçok bulgu vardır. Bu amaçla, streptozosinle diabet oluşturulan ratlara, glibenklamid (gliburid) uygulanmasından önce ve sonra, kas (M. gastrocnemius, M. soleus, M. quadriceps femoris) dokusunda oksidan savunma sistemi enzimlerinden olan katalazın aktivitesi incelendi. Bu araştırma için 150-200 gr ağırlığındaki ratlar kullanıldı. Ratlarda diabet oluşturmak amacıyla streptozotosin (55mg\kg), pH 4.5 sitrat tamponunda eritilerek intraperitonel olarak uygulandı. Elde edilen veriler bilgisayarda istatistik paket programı (SPSS 10,0) kullanılarak analiz edildi. Kas dokularında ölçülen katalaz aktivitelerinde diabet grubu kontrol grubuna göre her üç kasta da anlamlı derecede azalma (p<0.001) saptandı. Diabetik hayvanlara glibenklamid uygulanması ile bu enzimdeki azalmanın düzeldiği gözlendi. Sadece gastrocnemiusta ise glibenklamidin etkisinin olmadiği gözlendi. Glibenklamid’in azalmış katalaz düzeyini restore etmesinin mekanizması bu çalışmada incelenememiştir. Ancak çalışmaların sürdürülmesi, hem glibenklamidin restorasyonunun nedenlerine, hem de diabetin patogenezine katkısı olabileceği için gereklidir

Glibenklamidin sulfonylurea glyburide on catalase activities in streptozotocin-ınduced diabetic rat muscle

Purpose; Free radicals, those are chemical species possessing an unpaired electron in their molecular or atomic orbit, have been the most attractive subjects in medicine because of their roles in destruction of cell or tissue. Many findings support the view that free radicals and oxidative stress play an important role in etiology of diabetes and its complications. Materials and methods; Hence, in the present investigation, we administrated glyburide to streptozotosin-induced diabetic rats and determined the affect of glyburide on muscle (M. gastrocinemius, M. soleus, M. quadriceps femoris) catalase activities. Rats (Sprague Dawley), weighing 150- 200g were used in the present investigation. The experimental group was injected intraperitonally with streptozotosin (STZ, freshly dissolved in citrate buffer, pH 4.5 55 mg\kg) The proceeded data had been provided out from statisticaly (SPSS 10,0) based ware. Results; In the present study, muscle CAT activty was significantly reduced(p<0.001) in STZ-induced diabetic rats. Effecting glyburide treatment on diabetic rat muscles has been seen to make a measurable improvement creating a slight reduction on the decrease rate; however the above mentioned affect has not been traced on gastrocinemius muscles. Conclusion; This study could not cover an intention to find out the mechanism of restrotation of glyburide‘s decreasing affect level on catalayse. Further studies are needed to provide positive adds both for viewing the levels and mechanism of glyburide restration and expressing the the pathogenesis of type II diabetes.Moleküler veya atomik yörüngelerinde çiftleşmemiş e- bulunduran moleküller olan serbest radikaller; dokularda, hücre hasarı oluşumundaki rolleri ile, son yıllarda, tıbbın en ilgi çekici konularından biri durumuna gelmiştir. Diabet ve komplikasyonlarının oluşumunda, serbest radikaller ve oksidatif stresin rol oynayabileceğine ilişkin birçok bulgu vardır. Bu amaçla, streptozosinle diabet oluşturulan ratlara, glibenklamid (gliburid) uygulanmasından önce ve sonra, kas (M. gastrocnemius, M. soleus, M. quadriceps femoris) dokusunda oksidan savunma sistemi enzimlerinden olan katalazın aktivitesi incelendi. Bu araştırma için 150-200 gr ağırlığındaki ratlar kullanıldı. Ratlarda diabet oluşturmak amacıyla streptozotosin (55mg\kg), pH 4.5 sitrat tamponunda eritilerek intraperitonel olarak uygulandı. Elde edilen veriler bilgisayarda istatistik paket programı (SPSS 10,0) kullanılarak analiz edildi. Kas dokularında ölçülen katalaz aktivitelerinde diabet grubu kontrol grubuna göre her üç kasta da anlamlı derecede azalma (p<0.001) saptandı. Diabetik hayvanlara glibenklamid uygulanması ile bu enzimdeki azalmanın düzeldiği gözlendi. Sadece gastrocnemiusta ise glibenklamidin etkisinin olmadiği gözlendi. Glibenklamid’in azalmış katalaz düzeyini restore etmesinin mekanizması bu çalışmada incelenememiştir. Ancak çalışmaların sürdürülmesi, hem glibenklamidin restorasyonunun nedenlerine, hem de diabetin patogenezine katkısı olabileceği için gereklidir

Prospective, non-interventional, multicenter study of the intraocular pressure-lowering effects of prostaglandin analog/prostamide-containing therapies in previously treated patients with open-angle glaucoma or ocular hypertension

Objective: The objective of this study was to assess the intraocular pressure (IOP)-lowering efficacy, tolerability, safety, and usage patterns of prostaglandin analog/prostamide (PGA/P)containing topical ocular hypotensives in ocular hypertension (OHT) and primary open-angle glaucoma in the Turkish clinical setting

Prevalence of Ocular Surface Disease and Associated Risk Factors in Glaucoma Patients: A Survey Study of Ophthalmologists

Objectives: This survey study of ophthalmologists investigated the prevalence and clinical manifestations of ocular surface disease (OSD) in glaucoma patients, assessment methods used, risk factors, glaucoma drugs considered responsible, and treatment approaches. Materials and Methods: A questionnaire prepared jointly by the Turkish Ophthalmological Association Cornea and Ocular Surface Society and Glaucoma Society using SurveyMonkey was sent to ophthalmologists via e-mail. The distribution of parameters was compared with chi-square test and p < 0.05 was considered statistically significant. Results: Forty-five percent of the ophthalmologists reported that OSD was evident in least 25% of their patients. The most common symptom was redness (91.9%), while the most common ocular surface finding was conjunctival hyperemia (75.6%). The tests considered to be the most important in ocular surface assessment were ocular staining (38.7%) and tear film break-up time (TBUT) (21.9%). Ninety percent of the physicians stated that the main cause of OSD was benzalkonium chloride (BAC) in medications. Prostaglandin analogs and alpha-2 agonists were reported to be the most common medications causing OSD. In case of OSD, the ophthalmologists often switch to a glaucoma drug from a different group (38%), a non-preservative glaucoma drug (33.7%) or a drug with a preservative other than BAC (20.4%). Most physicians prescribed artificial tears (84.6%). Conclusion: In this cross-sectional survey study, ophthalmologists detected varying rates of OSD in glaucoma patients depending on chronic drug use and BAC exposure. Although ocular surface examination was performed by physicians, tests such as TBUT and ocular surface staining were rarely used. Detecting OSD in glaucoma patients and planning personalized treatment increase patient comfort, drug compliance, and treatment effectiveness. For this reason, it is important to prepare an algorithm for the management of comorbid OSD in glaucoma patients

Clinical and molecular evaluation of MEFV gene variants in the Turkish population: a study by the National Genetics Consortium

Familial Mediterranean fever (FMF) is a monogenic autoinflammatory disorder with recurrent fever, abdominal pain, serositis, articular manifestations, erysipelas-like erythema, and renal complications as its main features. Caused by the mutations in the MEditerranean FeVer (MEFV) gene, it mainly affects people of Mediterranean descent with a higher incidence in the Turkish, Jewish, Arabic, and Armenian populations. As our understanding of FMF improves, it becomes clearer that we are facing with a more complex picture of FMF with respect to its pathogenesis, penetrance, variant type (gain-of-function vs. loss-of-function), and inheritance. In this study, MEFV gene analysis results and clinical findings of 27,504 patients from 35 universities and institutions in Turkey and Northern Cyprus are combined in an effort to provide a better insight into the genotype-phenotype correlation and how a specific variant contributes to certain clinical findings in FMF patients. Our results may help better understand this complex disease and how the genotype may sometimes contribute to phenotype. Unlike many studies in the literature, our study investigated a broader symptomatic spectrum and the relationship between the genotype and phenotype data. In this sense, we aimed to guide all clinicians and academicians who work in this field to better establish a comprehensive data set for the patients. One of the biggest messages of our study is that lack of uniformity in some clinical and demographic data of participants may become an obstacle in approaching FMF patients and understanding this complex disease