23 research outputs found
Karakterizacija i toksiÄna aktivnost Aggregatibacter actinomycetemcomitans izolata
Periodontal diseases are widely distributed in the world and represent a major oral health problem both in developed and in developing countries. These chronic inflammatory diseases are characterized by the destruction of tooth supporting tissues. It is commonly accepted that dental plaque bacteria are the primary etiologic agents of periodontal disease. More than 700 species have been detected in the oral cavity in different individuals. Approximately 400 of these species have been isolated from different subgingival microenvironments. However, only a few species have been associated with the disease. From these bacteria specifically associated with destructive disease, Aggregatibacter actinomycetemcomitans is considered as one of the bacterial species of etiological importance in periodontitis and has contributed to the initiation and/or progression of destructive forms of periodontitis. The aim of this study was to evaluate the occurance characteristics and toxic activity of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Periodontal status of all included subjects was evaluated during the initial screening visit. A fullmouth clinical examination was performed in each patient using a manual probe and the following parameters were recorded at six sites per tooth: probing depth (PD in mm), clinical attachment loss (CAL in mm), bleeding on probing (BOP) and plaque index (PI). Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms and further molecular analysis. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using 16S rRNA gene sequencing. Genotyping was performed by polymerase chain reactions specific to the ltx promotor region, serotype-specific and cdt region and by sequencing of 16S rRNA. Cytotoxicity was examined on extravillous trophoblast cell line HTR-8/SVneo...Parodontalna oboljenja su Å”iroko rasprostanjena u celom svetu i u velikoj meri naruÅ”avaju oralno zdravlje, kako u razvijenim tako i u nerazvijenim zemljama. Parodontopatije su hroniÄna inflamatorna oboljenja potpornog aparata zuba koja usled razaranja dubljih parodontalnih tkiva mogu da rezultiraju gubitkom zuba. Etiologija parodontopatije je polimikrobna po svojoj prirodi. Opisano viÅ”e od 700 razliÄitih vrsta mikroorganizama koje naseljavaju usnu duplju, od kojih je oko 400 izolovano iz subgingivalne regije. MeÄutim, samo nekoliko bakterijskih vrsta je dovedeno u vezu sa parodontopatijama, meÄu kojima je i Aggregatibacter actinomycetemcomitans. Ovaj parodontopatogen se smatra veoma važnim u etiologiji parodontopatije, odnosno da doprinosi kako inicijaciji tako i progresiji destrukcije parodontalnih tkiva. Cilj ove studije je bio da se ispita prisustvo, karakteristike i toksiÄna aktivnost bakterije A. actinomycetemcomitans poreklom iz subgingivalnog dentalnog plaka, kod obolelih od parodontopatije kao i kod osoba sa kliniÄki zdravim parodoncijumom. Kod svih ispitanika ukljuÄenih u studiju, evidentiran je parodontalni status i nivo oralne higijene verifikacijom kliniÄkih parametara: dubinom sondiranja (DS u mm), nivoom pripojnog epitla (NPE u mm), krvarenjem na provokaciju (KNP) i plak indeksom (PI). Pulovani uzorci subgingivalnog dentalnog plaka su se koristili za kultivaciju i molekularne analize bakterije A. actinomycetemcomitans. Kultivacijom dobijene kolonije su selektovane u cilju identifikacije bakterije A. actinomycetemcomitans, sekvenciranjem 16S rRNK gena. KoriÅ”Äene su specifiÄne PCR reakcije u cilju genotipizacije promotorskog operona za leukotoksin, citoletalnog toksina istezanja i serotipizaciju kao i identifikaciju bakterije A. actinomycetemcomitans umnožavanjem dela gena za 16S rRNK. Ispitivan je uticaj bakterije A. actinomycetemcomitans na inhibiciju rasta ekstravilusne trofoblastne Äelijske linije HTR-8/SVneo..
Characterisation and toxic activity of Aggregatibacter actinomycetemcomitans isolates
Parodontalna oboljenja su Ŕiroko rasprostanjena u celom svetu i u velikoj meri
naruŔavaju oralno zdravlje, kako u razvijenim tako i u nerazvijenim zemljama.
Parodontopatije su hroniÄna inflamatorna oboljenja potpornog aparata zuba koja usled
razaranja dubljih parodontalnih tkiva mogu da rezultiraju gubitkom zuba. Etiologija
parodontopatije je polimikrobna po svojoj prirodi. Opisano viÅ”e od 700 razliÄitih vrsta
mikroorganizama koje naseljavaju usnu duplju, od kojih je oko 400 izolovano iz
subgingivalne regije. MeÄutim, samo nekoliko bakterijskih vrsta je dovedeno u vezu sa
parodontopatijama, meÄu kojima je i Aggregatibacter actinomycetemcomitans. Ovaj
parodontopatogen se smatra veoma važnim u etiologiji parodontopatije, odnosno da
doprinosi kako inicijaciji tako i progresiji destrukcije parodontalnih tkiva. Cilj ove
studije je bio da se ispita prisustvo, karakteristike i toksiÄna aktivnost bakterije A.
actinomycetemcomitans poreklom iz subgingivalnog dentalnog plaka, kod obolelih od
parodontopatije kao i kod osoba sa kliniÄki zdravim parodoncijumom. Kod svih
ispitanika ukljuÄenih u studiju, evidentiran je parodontalni status i nivo oralne higijene
verifikacijom kliniÄkih parametara: dubinom sondiranja (DS u mm), nivoom pripojnog
epitla (NPE u mm), krvarenjem na provokaciju (KNP) i plak indeksom (PI). Pulovani
uzorci subgingivalnog dentalnog plaka su se koristili za kultivaciju i molekularne
analize bakterije A. actinomycetemcomitans. Kultivacijom dobijene kolonije su
selektovane u cilju identifikacije bakterije A. actinomycetemcomitans, sekvenciranjem
16S rRNK gena. KoriÅ”Äene su specifiÄne PCR reakcije u cilju genotipizacije
promotorskog operona za leukotoksin, citoletalnog toksina istezanja i serotipizaciju kao
i identifikaciju bakterije A. actinomycetemcomitans umnožavanjem dela gena za 16S rRNK. Ispitivan je uticaj bakterije A. actinomycetemcomitans na inhibiciju rasta
ekstravilusne trofoblastne Äelijske linije HTR-8/SVneo...Periodontal diseases are widely distributed in the world and represent a major oral
health problem both in developed and in developing countries. These chronic
inflammatory diseases are characterized by the destruction of tooth supporting tissues. It
is commonly accepted that dental plaque bacteria are the primary etiologic agents of
periodontal disease. More than 700 species have been detected in the oral cavity in
different individuals. Approximately 400 of these species have been isolated from
different subgingival microenvironments. However, only a few species have been
associated with the disease. From these bacteria specifically associated with destructive
disease, Aggregatibacter actinomycetemcomitans is considered as one of the bacterial
species of etiological importance in periodontitis and has contributed to the initiation
and/or progression of destructive forms of periodontitis. The aim of this study was to
evaluate the occurance characteristics and toxic activity of A. actinomycetemcomitans
in the subgingival biofilm in subjects with periodontal health and disease. Periodontal
status of all included subjects was evaluated during the initial screening visit. A fullmouth
clinical examination was performed in each patient using a manual probe and the
following parameters were recorded at six sites per tooth: probing depth (PD in mm),
clinical attachment loss (CAL in mm), bleeding on probing (BOP) and plaque index
(PI). Pooled samples of subgingival plaque were taken for culture-based identification
of microorganisms and further molecular analysis. Colonies suspected to be A.
actinomycetemcomitans were selected for molecular identification using 16S rRNA
gene sequencing. Genotyping was performed by polymerase chain reactions specific to
the ltx promotor region, serotype-specific and cdt region and by sequencing of 16S
rRNA. Cytotoxicity was examined on extravillous trophoblast cell line HTR-8/SVneo..
Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted. [Projekat Ministarstva nauke Republike Srbije, br. 41008 and br. 173048
Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted
Multiple Major and Minor Anomalies Associated With Klippel-Feil Syndrome: A Case Report
Klippel-Feil syndrome is defined as congenital fusion of two or more cervical vertebrae. In this article, we report a 55-year-old male patient with one-year history of neck pain, headaches, and one episode of syncope after a severe trauma. X-rays and magnetic resonance imaging of cervical spine revealed fused vertebral bodies of C2-C5. The major anomalies associated with Klippel-Feil syndrome (small stature, thoracic kyphoscoliosis, lumbar scoliosis, restricted opening mouth, and bilateral sensorineural hearing loss) as well as multiple minor anomalies (mild face asymmetry, high arched palate, rhinoscoliosis, high nasal bridge, inclined septi nasi, and thin upper lip) were detected. This is a rare case describing the anomalies of the nose in Klippel-Feil syndrome patients. Our patient had no central cord impairment following a severe trauma
Procena periodontalnog zdravlja hospitalizovanih bolesnika sa shizofrenijom
Background/Aim. Many studies on oral health of psychiatric inpatients reported schizophrenia as the most common psychiatric disorder among their sample population. The available evidence suggests the higher prevalence and severity of periodontal disease among the psychiatric inpatients. The aim of this study was to evaluate periodontal health among the inpatients with schizophrenia and to consider possible risk factors for their current periodontal diseases. Methods. This cross-sectional study comprised 190 inpatients with schizophrenia at the Clinic for Psychiatric Disorders "Dr Laza LazareviÄ" in Belgrade, and 190 mentally healthy patients at the Clinic for Periodontology and Oral Medicine, Faculty of Dental Medicine, University in Belgrade. The Community Periodontal Index for Treatment Needs (CPITN) and sociodemographic characteristics were registered in both groups as well as the characteristics of the primary disease among the inpatients with schizophrenia. Results. The patients in the study group had significantly higher scores of the CPITN (2.24 Ā± 0.98) than the patients in the control group (1.21 Ā± 1.10). Most of the patients in the study group had supra, or subgingival calculi (46.8%), in contrast to the control group patients, who had in most cases gingival bleeding (45.8%). The periodontal pockets where detected in 35.8% of schizophrenic inpatients. The linear regression analysis showed that the gender and age were statistically significant predictors of the CPITN value among the inpatients with schizophrenia. Conclusion. The results of this study generally indicate the need for continuous research of psychiatric patients' oral health, in order to determine the modes of its improvement. Similar studies should elucidate significance of psychiatric patients' periodontal health and sensitize psychiatrists and psychiatric nurses to the oral problems of their patients.Uvod/Cilj. Mnogobrojna istraživanja oralnog zdravlju hospitalizovanih psihijatrijskih bolesnika pokazala su da je shizofrenija najÄeÅ”Äi psihijatrijski poremeÄaj meÄu njima. Dostupni podaci ukazuju na veliku prevalenciju i težinu periodontopatije kod bolesnika sa psihijatrijskim poremeÄajima. Cilj ove studije bio je da se proceni periodontalno zdravlje hospitalizovanih osoba sa shizofrenijom, kao i da se ukaže na moguÄe faktore rizika od oboljenja potpornog aparata zuba tih bolesnika. Metode. Studijom preseka obuhvaÄeno je 190 osoba sa shizofrenijom, hospitalizovanih u Klinici za psihijatrijske bolesti "Dr Laza LazareviÄ" u Beogradu i 190 mentalno zdravih osoba, pacijenata Klinike za parodontologiju i oralnu medicinu StomatoloÅ”kog fakultet, Univerziteta u Beogradu. U obe grupe ispitanika registrovane su vrednosti ZajedniÄki periodontni indeks potreba tretmana - Community Periodontal Index for Treatment Needs (CPITN), kao i sociodemografska obeležja, dok su karakteristike primarne bolesti beležene u studijskoj grupi. Rezultati. Ispitanici studijske grupe su imali znatno veÄe vrednosti CPITN (2,24 Ā± 0,98) u odnosu na ispitanike kontrolne grupe (1,21 Ā± 1,10). Kod veÄine ispitanika studijske grupe registrovano je prisustvo supra i subgingivalnog kamenca (46,8%), za razliku od ispitanika kontrolne grupe koji su ÄeÅ”Äe imali gingivalno krvarenje (45,8%). Periodontalni džepovi su registrovani kod 35,8% hospitalizovanih osoba sa shizofrenijom. Linearna regresiona analiza pokazala je da su pol i starost ispitanika bili statistiÄki znaÄajni prediktori vrednosti CPITN hospitalizovanih osoba sa shizofrenijom. ZakljuÄak. Rezultati ovog istraživanja ukazuju na postojanje potrebe za kontinuiranim istraživanjem stanja oralnog zdravlja psihijatrijskih bolesnika, kako bi se odredili naÄini za njegovo unapreÄenje. SliÄne studije bi trebalo da razjasne znaÄaj peridodontalnog zdravlja psihijatrijskih bolesnika, kako bi se psihijatrima i medicinskim sestrama ukazalo na postojanje problema oralnog zdravlja njihovih bolesnika
Efekat hlorheksidina na ekspresiju receptor aktivatora NF-kb liganda (RANKL) i osteoprotežerina (OPG) kod ljudi i kuÄnih ljubimaca
Periodontal disease is a chronic, multi-factorial disease of the tissues supporting the teeth. Periodontitis in companion animals is an almost identical disease to that in humans in terms of disease course and clinical presentation. Receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) are bioactive molecules that control bone resorption. This study aims to evaluate the effect of Chlorhexidine (CXD) on the RANKL and OPG expressions in gingival crevicular fluid (GCF) collected from subjects with chronic periodontitis. GCF was obtained from subjects with chronic periodontitis.10 subjects (CXD1) rinsed the mouth with 0.12% CXD, 10 subjects (CXD2) utilized 0.20% CXD and the last 10 (PL) used Placebo solution for 7 days. RANKL and OPG concentrations in GCF were measured by enzyme-linked immunosorbent assays ELISA at baseline and after 7 days. Periodontal clinical variables: clinical attachment loss (CAL), probing pocket depth (PPD), papilla-bleeding index (PBI) were evaluated in all groups. After 7 days in CXD1 and CXD2 group RANKL/OPG ratio exhibited a significant decrease (p lt 0.05) in contrast to the PL group where results showed similar values of RANKL/OPG ratio at baseline and after the observation period. RANKL/OPG ratio was positively correlated with PPD, CAL and PBI before and after the observation period in both Chlorhexidine (CXD1, CXD2) groups. In an existing inflammatory response, chlorhexidine reduced the level of periodontal inflammation, which leads to reduction of RANKL/OPG relative ratio. Decrease of RANKL/OPG ratio will apparently induce maintenance of alveolar bone and slow down periodontal tissue breakdown.Parodontopatije su hroniÄna, multikauzalna oboljenja potpornog aparata zuba. Parodontalna oboljenja koja sreÄemo kod kuÄnih ljubimaca su prema toku i kliniÄkoj slici skoro identiÄna onima koje se javljaju kod ljudi. RANKL i osteoprotegerin (OPG) su bioaktivni molekuli koji kontroliÅ”u koÅ”tanu resorpciju. Cilj ove studije je evaluacija efekata hlorheksidina na ekspresiju RANKL-a i OPG-a u gingivalnoj teÄnosti (GT) uzetoj od pacijenata sa hroniÄnom parodontopatijom. 10 pacijenata (CXD1) su ispirali usta sa 0.12% CXD, 10 pacijenata (CXD2) su koristili 0.20% CXD i poslednjih 10 pacijenata (PL) su koristili placebo rastvor 7 dana. RANKL i OPG koncentracije u GT su merene ELISA testom na poÄetku i posle sedam dana. Parodontalni kliniÄki parametri CAL, PPD i PBI su evaluirani u svim grupama. Posle 7 dana u CXD1 i CXD2 grupi RANKL/OPG odnos je pokazao signifikantno smanjenje (p lt 0.05) u poreÄenju sa PL grupom gde su zabaleženi sliÄni rezultati na poÄetku i nakon opservacionog perioda. RANKL/OPG odnos je pokazao pozitivnu korelaciju sa vrednostima PPD-a, CAL-a i PBI-a pre i nakon observacionog perioda u obe eksperimentalne grupe (CXD1, CXD2). U prisutnom inflamatornom odgovoru hlorheksidin je redukovao nivo inflamacije, Å”to je uslovilo redukciju RANKL/OPG odnosa. Rezultati istraživanja dokazuju da koncentracija hlorheksidina ne utiÄe statistiÄki znaÄajno na smanjenje RANKL/OPG odnosa
Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings
Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues
Identifikacija parodontopatogenih mikroorganizama PCR tehnikom
INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.Uvod EpidemioloÅ”ki podaci iz Äitavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma kljuÄnu ulogu igraju razliÄiti rodovi Gram-negativnih bakterija, ponajviÅ”e striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u razliÄitim uzorcima poreklom iz usne duplje pacijenata s kliniÄki dijagnostikovanom parodontopatijom. Metod rada Kao bioloÅ”ki materijal u kojem je dokazivano postojanje DNK mikroorganizama koriÅ”Äeni su zubni plak, tkivo zapaljene gingive i pljuvaÄka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lanÄanog umnožavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve razliÄite bakterije. Rezultati S manjom ili veÄom uÄestaloÅ”Äu, u svim ispitanim uzorcima utvrÄeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najÄeÅ”Äi je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najveÄem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, Å”to je odlika hroniÄnog oblika parodontopatije, a u pljuvaÄki ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapaženo u pljuvaÄki. ZakljuÄak Primenjeni metod PCR ima veliku osetljivost i specifiÄnost. Brzo i precizno otkrivanje mikroorganizama je veoma važno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i leÄenje parodontopatija. U svakodnevnoj kliniÄkoj praksi optimalan bioloÅ”ki materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu