Testing the Hubble Law with the IRAS 1.2 Jy Redshift Survey

We test and reject the claim of Segal et al. (1993) that the correlation of redshifts and flux densities in a complete sample of IRAS galaxies favors a quadratic redshift-distance relation over the linear Hubble law. This is done, in effect, by treating the entire galaxy luminosity function as derived from the 60 micron 1.2 Jy IRAS redshift survey of Fisher et al. (1995) as a distance indicator; equivalently, we compare the flux density distribution of galaxies as a function of redshift with predictions under different redshift-distance cosmologies, under the assumption of a universal luminosity function. This method does not assume a uniform distribution of galaxies in space. We find that this test has rather weak discriminatory power, as argued by Petrosian (1993), and the differences between models are not as stark as one might expect a priori. Even so, we find that the Hubble law is indeed more strongly supported by the analysis than is the quadratic redshift-distance relation. We identify a bias in the the Segal et al. determination of the luminosity function, which could lead one to mistakenly favor the quadratic redshift-distance law. We also present several complementary analyses of the density field of the sample; the galaxy density field is found to be close to homogeneous on large scales if the Hubble law is assumed, while this is not the case with the quadratic redshift-distance relation.Comment: 27 pages Latex (w/figures), ApJ, in press. Uses AAS macros, postscript also available at http://www.astro.princeton.edu/~library/preprints/pop682.ps.g

Missing in Action? Electronic Gaming Machines in Gambling Studies Research

In the past thirty years casinos across the world have become dominated by the rise of “electronic gaming machines” (EGMs). Expanding with tremendous speed, this technology has arguably become the dominant form of non-online gambling around the world at time of writing (DeMichele, 2017; Schwartz, 2018). EGMs are also noted as being one of the most harmful forms of gambling, with significant numbers of players betting beyond their financial limits (MacLaren et al, 2012; Stewart & Wohl, 2013), spending a disproportionate amount of time playing (Cummings, 1999; Ballon, 2005; Schüll, 2012; cf. Dickerson, 1996), becoming disconnected from the world outside of the “zone” (Schüll, 2012) of gambling play, and even becoming bankrupt or otherwise financially crippled as a result of their use (Petry, 2003; Scarf et al, 2011). Using metadata from Web of Science and Scopus databases, we analysed peer-reviewed gambling research produced in Australia, New Zealand, North America and the UK published between 1996 and 2016. Surprisingly, we found that the overwhelming of majority of articles do not specifically address EGMs as the most popular and pervasive gambling technology available. Our paper teases out some concerning implications of this finding for the interdisciplinary field of gambling studies

An investigation of herpes simplex virus promoter activity compatible with latency establishment reveals VP16-independent activation of immediate-early promoters in sensory neurones

Herpes simplex virus (HSV) type-1 establishes lifelong latency in sensory neurones and it is widely assumed that latency is the consequence of a failure to initiate virus immediate-early (IE) gene expression. However, using a Ore reporter mouse system in conjunction with Ore-expressing HSV-1 recombinants we have previously shown that activation of the IE ICPO promoter can precede latency establishment in at least 30 % of latently infected cells. During productive infection of non-neuronal cells, IE promoter activation is largely dependent on the transactivator VP16 a late structural component of the virion. Of significance, VP16 has recently been shown to exhibit altered regulation in neurones; where its de novo synthesis is necessary for IE gene expression during both lytic infection and reactivation from latency. In the current study, we utilized the Ore reporter mouse model system to characterize the full extent of viral promoter activity compatible with cell survival and latency establishment. In contrast to the high frequency activation of representative IE promoters prior to latency establishment, cell marking using a virus recombinant expressing Ore under VP16 promoter control was very inefficient. Furthermore, infection of neuronal cultures with VP16 mutants reveals a strong VP16 requirement for IE promoter activity in non-neuronal cells, but not sensory neurones. We conclude that only IE promoter activation can efficiently precede latency establishment and that this activation is likely to occur through a VP16-independent mechanism

Critical Perspective: The Unnatural Nature of the Animal Rights/Liberation Philosophy

Author Institution: Department of Integrative Biology and Cancer Research Laboratory, University of California at Berkele

Stratigraphy and Conodont Paleontology of the Salamonie Dolomite and Lee Creek Member of the Brassfield Limestone (Silurian) In Southeastern Indiana and Adjacent Kentucky

Indiana Geological Survey Bulletin 40Zonation established by study of the conodont faunas of the Lee Creek Member (new member) of the Brassfield Limestone and of the Salamonie Dolomite, both of Silurian age, from 42 sections in southeastern Indiana and north-central Kentucky differs from the conodont zonation established by O. H. Walliser in 1964 for the lower Silurian and lower part of the middle Silurian rocks of the Carnic Alps of Europe. Three conodont assemblage zones are named. In ascending order these are the Icriodina irregularis Assemblage Zone, the Neospathognathodus celloni Assemblage Zone, and the Pterospathodus amorphognathoides-Spathognathodus ranuliformis Assemblage Zone. These zones correspond in general with the upper part of Walliser’s Bereich I and with his celloni-and amorphognathoides-Zones. The new name Lee Creek Member is applied to a thin dolomite unit at the top of the Brassfield Limestone. Fifty-nine named species, 16 of them new, belonging to 22 genera, two of them new, were identified from about 8,900 specimens obtained in this study. The new taxa include the genera Diadelognathus and Neospathognathodus and the species Diadelognathus compressus, D. excertus, D. primus, Drepanodus aduncus, Ligonodina petila, Neospathognathodus bullatus, N. ceratoides, N. latus, Ozarkodina hanoverensis, 0. neogaertneri, Spathognathodus hadros, S. polinclinatus, Synprioniodina? variabilis, Trichonodella asymmetrica, T.? expansa, and T. papilio. Two species each of Diadelognathus and of Paltodus, and one each of Carniodus, Distacodus?, and Trichonodella are described but not named.Indiana Department of Natural Resource

Photo-Crosslinked Alginate Hydrogels Support Enhanced Matrix Accumulation by Nucleus Pulposus Cells in Vivo

Objective Intervertebral disc (IVD) degeneration is a major health concern in the United States. Replacement of the nucleus pulposus (NP) with injectable biomaterials represents a potential treatment strategy for IVD degeneration. The objective of this study was to characterize the extracellular matrix (ECM) assembly and functional properties of NP cell-encapsulated, photo-crosslinked alginate hydrogels in comparison to ionically crosslinked alginate constructs. Methods Methacrylated alginate was synthesized by esterification of hydroxyl groups with methacrylic anhydride. Bovine NP cells were encapsulated in alginate hydrogels by ionic crosslinking using CaCl2 or through photo-crosslinking upon exposure to long-wave UV light in the presence of a photoinitiator. The hydrogels were evaluated in vitro by gross and histological analysis and in vivo using a murine subcutaneous pouch model. In vivo samples were analyzed for gene expression, ECM localization and accumulation, and equilibrium mechanical properties. Results Ionically crosslinked hydrogels exhibited inferior proteoglycan accumulation in vitro and were unable to maintain structural integrity in vivo. In further studies, photo-crosslinked alginate hydrogels were implanted for up to 8 weeks to examine NP tissue formation. Photo-crosslinked hydrogels displayed temporal increases in gene expression and assembly of type II collagen and proteoglycans. Additionally, hydrogels remained intact over the duration of the study and the equilibrium Young\u27s modulus increased from 1.24 ± 0.09 kPa to 4.31 ± 1.39 kPa, indicating the formation of functional matrix with properties comparable to those of the native NP. Conclusions These findings support the use of photo-crosslinked alginate hydrogels as biomaterial scaffolds for NP replacement

The CaMKII/NMDA receptor complex controls hippocampal synaptic transmission by kinase-dependent and independent mechanisms.

CaMKII is one of the most studied synaptic proteins, but many critical issues regarding its role in synaptic function remain unresolved. Using a CRISPR-based system to delete CaMKII and replace it with mutated forms in single neurons, we have rigorously addressed its various synaptic roles. In brief, basal AMPAR and NMDAR synaptic transmission both require CaMKIIα, but not CaMKIIβ, indicating that, even in the adult, synaptic transmission is determined by the ongoing action of CaMKIIα. While AMPAR transmission requires kinase activity, NMDAR transmission does not, implying a scaffolding role for the CaMKII protein instead. LTP is abolished in the absence of CaMKIIα and/or CaMKIIβ and with an autophosphorylation impaired CaMKIIα (T286A). With the exception of NMDAR synaptic currents, all aspects of CaMKIIα signaling examined require binding to the NMDAR, emphasizing the essential role of this receptor as a master synaptic signaling hub

Author Correction: The CaMKII/NMDA receptor complex controls hippocampal synaptic transmission by kinase-dependent and independent mechanisms.

The originally published version of this Article contained errors in Figure 5, for which we apologise. In panel c, the scatter graph was inadvertently replaced with a scatter graph comprising a subset of data points from panel d. Furthermore, the legends to Figures 5c and 5d were inverted. These errors have now been corrected in both the PDF and HTML versions of the Article, and the incorrect version of Fig. 5c is presented in the Author Correction associated with this Article