Stratigraphy and Conodont Paleontology of the Salamonie Dolomite and Lee Creek Member of the Brassfield Limestone (Silurian) In Southeastern Indiana and Adjacent Kentucky

Indiana Geological Survey Bulletin 40Zonation established by study of the conodont faunas of the Lee Creek Member (new member) of the Brassfield Limestone and of the Salamonie Dolomite, both of Silurian age, from 42 sections in southeastern Indiana and north-central Kentucky differs from the conodont zonation established by O. H. Walliser in 1964 for the lower Silurian and lower part of the middle Silurian rocks of the Carnic Alps of Europe. Three conodont assemblage zones are named. In ascending order these are the Icriodina irregularis Assemblage Zone, the Neospathognathodus celloni Assemblage Zone, and the Pterospathodus amorphognathoides-Spathognathodus ranuliformis Assemblage Zone. These zones correspond in general with the upper part of Walliser’s Bereich I and with his celloni-and amorphognathoides-Zones. The new name Lee Creek Member is applied to a thin dolomite unit at the top of the Brassfield Limestone. Fifty-nine named species, 16 of them new, belonging to 22 genera, two of them new, were identified from about 8,900 specimens obtained in this study. The new taxa include the genera Diadelognathus and Neospathognathodus and the species Diadelognathus compressus, D. excertus, D. primus, Drepanodus aduncus, Ligonodina petila, Neospathognathodus bullatus, N. ceratoides, N. latus, Ozarkodina hanoverensis, 0. neogaertneri, Spathognathodus hadros, S. polinclinatus, Synprioniodina? variabilis, Trichonodella asymmetrica, T.? expansa, and T. papilio. Two species each of Diadelognathus and of Paltodus, and one each of Carniodus, Distacodus?, and Trichonodella are described but not named.Indiana Department of Natural Resource

The conodont genus Teridontus (Miller, 1980) from the Early Ordovician of Montagne Noire, France

THE CONODONT genus Teridontus was introduced in 1980 by Miller and was based on the Late Cambrian species Oneotodus nakamurai Nogami, 1967 from the Yencho Member of the Fengshan Fm. of northeast China. Teridontus was later reported from either the Upper Cambrian or Lower Ordovician (Landing et al., 1980; Miller, 1980; Landing and Barnes, 1981; Landing,1983; An et al., 1983, 1985; Ni et al., 1983; Peng et al., 1983; Nowlan, 1985; Landing et al., 1986; Bagnoli et al., 1987; An, 1987; Buggisch and Repetski, 1987; Pohler and Orchard, 1990; An and Zheng, 1990; Seo and Ethington, 1993; Wang, 1993; Lehnert, 1994; Nicoll, 1994; Seo et al., 1994; Ji and Barnes, 1994; Taylor et al., 1996; Lehnert et al., 1997; Jia, 2000; Dubinina, 2000; Pyle and Barnes, 2002; Zeballo et al., 2005) sediments in numerous localities around the world, but a unanimous interpretation of the composition of the Teridontus apparatus organization was far from accepted

Ichnofacies of the Stairway Sandstone fish-fossil beds (Middle Ordovician, Northern Territory, Australia)

The Stairway Sandstone is a 30-560 m thick succession of Middle Ordovician siliciclastic sedimentary rocks within the Amadeus Basin of central Australia, deposited in the epeiric Larapintine Sea of northern peri-Gondwana. The Stairway Sandstone is significant as one of only two known Gondwanan successions to yield articulated arandaspid (pteraspidomorph agnathan) fish. Herein we use the ichnology of the Stairway Sandstone to reveal insights into the shallow marine habitat of these early vertebrates, and compare it with that of other known pteraspidomorph-bearing localities from across Gondwana. The Stairway Sandstone contains a diverse Ordovician ichnofauna including 22 ichnotaxa of Arenicolites, Arthrophycus, Asterosoma, Cruziana, Didymaulichnus, Diplichnites, Diplocraterion,? Gordia, Lockeia, Monocraterion, Monomorphichnus, Phycodes, Planolites, Rusophycus, Skolithos and Uchirites. These ichnofauna provide a well-preserved example of a typical Ordovician epeiric sea assemblage, recording the diverse ethologies of tracemakers in a very shallow marine environment of flashy sediment accumulation and regularly shifting sandy substrates. New conodont data refine the age of the Stairway Sandstone to the early Darriwilian, with ichnostratigraphic implications for the Cruziana rugosa group and Arthrophycus alleghaniensis

A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry.

Psychoactive compounds such as chloroquine and amphetamine act by dissipating the pH gradient across intracellular membranes, but the physiological mechanisms that normally regulate organelle pH remain poorly understood. Interestingly, recent human genetic studies have implicated the endosomal Na+/H+ exchanger NHE9 in both autism spectrum disorders (ASD) and attention deficit hyperactivity disorder (ADHD). Plasma membrane NHEs regulate cytosolic pH, but the role of intracellular isoforms has remained unclear. We now find that inactivation of NHE9 in mice reproduces behavioral features of ASD including impaired social interaction, repetitive behaviors, and altered sensory processing. Physiological characterization reveals hyperacidic endosomes, a cell-autonomous defect in glutamate receptor expression and impaired neurotransmitter release due to a defect in presynaptic Ca2+ entry. Acute inhibition of synaptic vesicle acidification rescues release but without affecting the primary defect due to loss of NHE9

Functional studies and distribution define a family of transmembrane AMPA receptor regulatory proteins

Functional expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in cerebellar granule cells requires stargazin, a member of a large family of four-pass transmembrane proteins. Here, we define a family of transmembrane AMPA receptor regulatory proteins (TARPs), which comprise stargazin, γ-3, γ-4, and γ-8, but not related proteins, that mediate surface expression of AMPA receptors. TARPs exhibit discrete and complementary patterns of expression in both neurons and glia in the developing and mature central nervous system. In brain regions that express multiple isoforms, such as cerebral cortex, TARP–AMPA receptor complexes are strictly segregated, suggesting distinct roles for TARP isoforms. TARPs interact with AMPA receptors at the postsynaptic density, and surface expression of mature AMPA receptors requires a TARP. These studies indicate a general role for TARPs in controlling synaptic AMPA receptors throughout the central nervous system

Gambling and problem gambling in Canada in 2018: prevalence and changes since 2002

Permission to archive accepted author manuscript. Reuse is restricted to non-commercial and no derivative uses.Objective The purpose of this study was to provide an updated profile of gambling and problem gambling in Canada and to examine how the rates and pattern of participation compare to 2002. Method An assessment of gambling and problem gambling was included in the 2018 Canadian Community Health Survey and administered to 24,982 individuals aged 15 and older. The present analyses selected for adults (18+). Results A total of 66.2% of people reported engaging in some type of gambling in 2018, primarily lottery and/or raffle tickets, the only type in which the majority of Canadians participate. There are some significant inter-provincial differences, with perhaps the most important one being the higher rate of electronic gambling machine (EGM) participation in Manitoba and Saskatchewan The overall pattern of gambling in 2018 is very similar to 2002, although participation is generally much lower in 2018, particularly for EGMs and bingo. Only 0.6% of the population were identified as problem gamblers in 2018, with an additional 2.7% being at-risk gamblers. There is no significant inter-provincial variation in problem gambling rates. The inter-provincial pattern of problem gambling in 2018 is also very similar to what was found in 2002 with the main difference being a 45% decrease in the overall prevalence of problem gambling. Conclusions Gambling and problem gambling have both decreased in Canada from 2002 to 2018, although the provincial patterns are quite similar between the two time periods. Several mechanisms have likely collectively contributed to these declines. Decreases have also been reported in several other western countries in recent years and have occurred despite the expansion of legal gambling opportunities, suggesting a degree of inoculation or adaptation in the population.Ye

Motor activity improves temporal expectancy

Certain brain areas involved in interval timing are also important in motor activity. This raises the possibility that motor activity might influence interval timing. To test this hypothesis, we assessed interval timing in healthy adults following different types of training. The pre- and post-training tasks consisted of a button press in response to the presentation of a rhythmic visual stimulus. Alterations in temporal expectancy were evaluated by measuring response times. Training consisted of responding to the visual presentation of regularly appearing stimuli by either: (1) pointing with a whole-body movement, (2) pointing only with the arm, (3) imagining pointing with a whole-body movement, (4) simply watching the stimulus presentation, (5) pointing with a whole-body movement in response to a target that appeared at irregular intervals (6) reading a newspaper. Participants performing a motor activity in response to the regular target showed significant improvements in judgment times compared to individuals with no associated motor activity. Individuals who only imagined pointing with a whole-body movement also showed significant improvements. No improvements were observed in the group that trained with a motor response to an irregular stimulus, hence eliminating the explanation that the improved temporal expectations of the other motor training groups was purely due to an improved motor capacity to press the response button. All groups performed a secondary task equally well, hence indicating that our results could not simply be attributed to differences in attention between the groups. Our results show that motor activity, even when it does not play a causal or corrective role, can lead to improved interval timing judgments

The Biochemistry, Ultrastructure, and Subunit Assembly Mechanism of AMPA Receptors

The AMPA-type ionotropic glutamate receptors (AMPA-Rs) are tetrameric ligand-gated ion channels that play crucial roles in synaptic transmission and plasticity. Our knowledge about the ultrastructure and subunit assembly mechanisms of intact AMPA-Rs was very limited. However, the new studies using single particle EM and X-ray crystallography are revealing important insights. For example, the tetrameric crystal structure of the GluA2cryst construct provided the atomic view of the intact receptor. In addition, the single particle EM structures of the subunit assembly intermediates revealed the conformational requirement for the dimer-to-tetramer transition during the maturation of AMPA-Rs. These new data in the field provide new models and interpretations. In the brain, the native AMPA-R complexes contain auxiliary subunits that influence subunit assembly, gating, and trafficking of the AMPA-Rs. Understanding the mechanisms of the auxiliary subunits will become increasingly important to precisely describe the function of AMPA-Rs in the brain. The AMPA-R proteomics studies continuously reveal a previously unexpected degree of molecular heterogeneity of the complex. Because the AMPA-Rs are important drug targets for treating various neurological and psychiatric diseases, it is likely that these new native complexes will require detailed mechanistic analysis in the future. The current ultrastructural data on the receptors and the receptor-expressing stable cell lines that were developed during the course of these studies are useful resources for high throughput drug screening and further drug designing. Moreover, we are getting closer to understanding the precise mechanisms of AMPA-R-mediated synaptic plasticity

Multiorgan MRI findings after hospitalisation with COVID-19 in the UK (C-MORE): a prospective, multicentre, observational cohort study

Introduction: The multiorgan impact of moderate to severe coronavirus infections in the post-acute phase is still poorly understood. We aimed to evaluate the excess burden of multiorgan abnormalities after hospitalisation with COVID-19, evaluate their determinants, and explore associations with patient-related outcome measures. Methods: In a prospective, UK-wide, multicentre MRI follow-up study (C-MORE), adults (aged ≥18 years) discharged from hospital following COVID-19 who were included in Tier 2 of the Post-hospitalisation COVID-19 study (PHOSP-COVID) and contemporary controls with no evidence of previous COVID-19 (SARS-CoV-2 nucleocapsid antibody negative) underwent multiorgan MRI (lungs, heart, brain, liver, and kidneys) with quantitative and qualitative assessment of images and clinical adjudication when relevant. Individuals with end-stage renal failure or contraindications to MRI were excluded. Participants also underwent detailed recording of symptoms, and physiological and biochemical tests. The primary outcome was the excess burden of multiorgan abnormalities (two or more organs) relative to controls, with further adjustments for potential confounders. The C-MORE study is ongoing and is registered with ClinicalTrials.gov, NCT04510025. Findings: Of 2710 participants in Tier 2 of PHOSP-COVID, 531 were recruited across 13 UK-wide C-MORE sites. After exclusions, 259 C-MORE patients (mean age 57 years [SD 12]; 158 [61%] male and 101 [39%] female) who were discharged from hospital with PCR-confirmed or clinically diagnosed COVID-19 between March 1, 2020, and Nov 1, 2021, and 52 non-COVID-19 controls from the community (mean age 49 years [SD 14]; 30 [58%] male and 22 [42%] female) were included in the analysis. Patients were assessed at a median of 5·0 months (IQR 4·2–6·3) after hospital discharge. Compared with non-COVID-19 controls, patients were older, living with more obesity, and had more comorbidities. Multiorgan abnormalities on MRI were more frequent in patients than in controls (157 [61%] of 259 vs 14 [27%] of 52; p<0·0001) and independently associated with COVID-19 status (odds ratio [OR] 2·9 [95% CI 1·5–5·8]; padjusted=0·0023) after adjusting for relevant confounders. Compared with controls, patients were more likely to have MRI evidence of lung abnormalities (p=0·0001; parenchymal abnormalities), brain abnormalities (p<0·0001; more white matter hyperintensities and regional brain volume reduction), and kidney abnormalities (p=0·014; lower medullary T1 and loss of corticomedullary differentiation), whereas cardiac and liver MRI abnormalities were similar between patients and controls. Patients with multiorgan abnormalities were older (difference in mean age 7 years [95% CI 4–10]; mean age of 59·8 years [SD 11·7] with multiorgan abnormalities vs mean age of 52·8 years [11·9] without multiorgan abnormalities; p<0·0001), more likely to have three or more comorbidities (OR 2·47 [1·32–4·82]; padjusted=0·0059), and more likely to have a more severe acute infection (acute CRP >5mg/L, OR 3·55 [1·23–11·88]; padjusted=0·025) than those without multiorgan abnormalities. Presence of lung MRI abnormalities was associated with a two-fold higher risk of chest tightness, and multiorgan MRI abnormalities were associated with severe and very severe persistent physical and mental health impairment (PHOSP-COVID symptom clusters) after hospitalisation. Interpretation: After hospitalisation for COVID-19, people are at risk of multiorgan abnormalities in the medium term. Our findings emphasise the need for proactive multidisciplinary care pathways, with the potential for imaging to guide surveillance frequency and therapeutic stratification

A histochemical analysis of biological residues in conodont elements

A series of histochemical tests have been performed on decalcified conodont elements of five species of conodont, Erratocogon patu, Prioniodus amadeus, Cordylodus spp. Palmatolepis spp. and Polygnathus spp. These tests show that, although the hyaline tissue varies in microstructure among the genera tested, all show a histochemical reaction for collagen. Vertebrate enamel does not possess collagen, and the hyaline tissue is therefore not an equivalent tissue to enamel. In all five conodont genera tested, the albid tissue, possessing lacunae and an inorganic matrix, does not stain for collagen. An organic matrix of collagen is required if this tissue is to be described as bone, and albid tissue and vertebrate bone cannot be equated. Albid tissue does possess lacunae, but these are too small to have housed eukaryotic cells, and moreover, do not contain cell remnants. The apparent 'orthodentine' found in Cordylodus and in P. amadeus does not have the detailed histological structure expected of vertebrate orthodentine, and is not associated with cells. The only traces of cell residues found in conodont elements were localised to the attachment cone, amongst a notochord - like tissue, in three of the conodont species tested, Cordylodus spp., E. patu and P. amadeus. The attachment cone reacted as if it contained chondroitin sulphate and collagen, but the histological appearance indicates that it does not consist of spheritic calcified cartilage. Results of this study are consistent with the view that the actual relationships of the conodont animal rest with the cephalochordates, or animals closely related to this group. They do not support chaetognath, vertebrate or agnathan links