Difficult airway management in an ankylosing spondylitis case using video laryngoscope: a case report

Ankylosing spondylitis (AS) known as autoimmune disease with chronic inflammation within vertebral bone, characterized with bamboo spine. AS with cervical and temporomandibular joint involvement can lead to difficult airway. In this report, we present an AS patient with severe ARDS (acute respiratory distress syndrome) due to COVID-19 who was successfully intubated using video laryngoscope. A-37 years old male, came to emergency room in agitated condition, history of AS with cervical spine and temporomandibular joint involvement, history of pulmonary tuberculosis in 2008, and haven’t been vaccinated (COVID-19). This patient was diagnosed with severe ARDS due to COVID-19 with GCS 3-1-3, respiratory rate 36 times per minute, maximum peripheral saturation only 90% with Jackson Rees and pCO2 103 mmHg. These conditions are indication for endotracheal intubation. With the aid of video laryngoscope, endotracheal intubation was successfully performed. Improvements of peripheral saturation and blood gas analysis were obtained after intubation. This patient was included in the criteria for intubation due to respiratory rate over 30 times per minute and pCO2 more than 100 mmHg. Based on difficult airway predictor (MOANS, LEMON, RODS and SHORT), this patient was categorized as hard to ventilate and hard to intubate so it is necessary to prepare supraglottic airway device if intubation fails or surgical cricothyrotomy if intubation and supraglottic device fail. In difficult airway conditions, endotracheal intubation is ideally performed without muscle relaxants or awake intubation. The use of video laryngoscope is a recommended procedure in difficult airway management as in AS patient or in head and neck trauma cases. The availability of video laryngoscope in every hospital will maximize airway management in emergency room in Indonesia

Large expert-curated database for benchmarking document similarity detection in biomedical literature search

Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we have established the RElevant LIterature SearcH consortium consisting of more than 1500 scientists from 84 countries, who have collectively annotated the relevance of over 180 000 PubMed-listed articles with regard to their respective seed (input) article/s. The majority of annotations were contributed by highly experienced, original authors of the seed articles. The collected data cover 76% of all unique PubMed Medical Subject Headings descriptors. No systematic biases were observed across different experience levels, research fields or time spent on annotations. More importantly, annotations of the same document pairs contributed by different scientists were highly concordant. We further show that the three representative baseline methods used to generate recommended articles for evaluation (Okapi Best Matching 25, Term Frequency-Inverse Document Frequency and PubMed Related Articles) had similar overall performances. Additionally, we found that these methods each tend to produce distinct collections of recommended articles, suggesting that a hybrid method may be required to completely capture all relevant articles. The established database server located at https://relishdb.ict.griffith.edu.au is freely available for the downloading of annotation data and the blind testing of new methods. We expect that this benchmark will be useful for stimulating the development of new powerful techniques for title and title/abstract-based search engines for relevant articles in biomedical science.</p

Multiscale Mechanical Characterization of Soft Matter

Ph.DDOCTOR OF PHILOSOPH

PENGARUH INTENSITAS MENONTON FILM PADA PLATFORM LAYANAN STREAMING NETFLIX TERHADAP MINAT MENONTON FILM DI BIOSKOP (Studi Kuantitatif Eksplanatif dengan Metode Survei pada Masyarakat Umum Usia 18-25 Tahun di Kabupaten Sleman, Yogyakarta)

Penelitian ini bertujuan untuk melihat pengaruh intensitas menonton film pada platform layanan streaming Netflix terhadap minat menonton film di bioskop. Permasalahan ini diangkat dari isu maraknya platform layanan streaming film khususnya Netflix, yang menunjukkan peningkatan pesat selama masa pandemi Covid-19. Pengambilan subjek masyarakat umum usia 18-25 tahun didasarkan pada survei preferensi usia terbanyak penonton film di bioskop dan pembatasan usia pengguna Netflix. Selain itu, Kabupaten Sleman juga dipilih karena heterogenitas masyarakat yang tinggi. Peneliti menggunakan teori uses and gratifications dan konsep konsumen media untuk memperjelas hubungan kedua variabel ini. Jenis penelitian yang digunakan adalah kuantitatif eksplanatif dengan metode survei melalui google form yang menyasar 100 orang responden. Dalam penelitian ini, uji validitas, reliabilitas, dan data hasil penelitian diuji menggunakan IBM SPSS Statistics versi 25.0. Untuk teknik analisis yang digunakan adalah korelasi pearson product moment dan regresi linier sederhana. Hasil penelitian menunjukkan bahwa minat menonton film di bioskop dipengaruhi sebesar 46,8% oleh intensitas menonton film di Netflix dan sifat hubungannya bernilai positif. Kedua variabel juga berada pada kategori tinggi, dengan variabel X yaitu intensitas menonton memperoleh hasil sebesar 72,9%, dan variabel Y yaitu minat menonton memperoleh hasil sebesar 75,1%. Selain itu, hubungan kedua variabel juga bersifat kuat, dilihat dari hasil nilai korelasi yang didapat yaitu sebesar 0,684

Perspektif Orang Tua Terhadap Game Online

Game online merupakan permainan video yang bisa dimainkan dalam bentuk berbagai perangkat dan membutuhkan jaringan. Tujuan dari penelitian ini adalah mengetahui perspektif orang tua terhadap game online. Jenis penelitian ini menggunakan metode deskriptif kuantitatif. Pengambilan data dengan menyebarkan kuesioner menggunakan google form sebagai medianya dan diperoleh 35 orang tua mahasiswa Universitas Atma Jaya Yogyakarta angkatan 2019, 2020, dan 2021 serta dengan rata-rata hasil uji reliabilitas sebesar 0.855. Hasil penelitian ini menunjukkan bahwa orang tua yang menjadi objek penelitian ini didominasi oleh orang tua yang berjenjang pendidikan terakhir S1/sarjana sebesar 49 %, persepsi orang tua mengenai interaksi mahasiswa dengan game online cukup besar yang dimana tingkat kesenangan mahasiswa dalam bermain game online sebagian besar didominasi skala ordinal 4 = setuju dan waktu yang dihabiskan mahasiswa bermain game online dalam sehari sebagian besar lebih dari 3 jam, dan perspektif orang tua terhadap interaksi tersebut didominasi oleh skala ordinal 3 = netral

SegmentAnything helps microscopy images based automatic and quantitative organoid detection and analysis

Organoids are self-organized 3D cell clusters that closely mimic the architecture and function of in vivo tissues and organs. Quantification of organoid morphology helps in studying organ development, drug discovery, and toxicity assessment. Recent microscopy techniques provide a potent tool to acquire organoid morphology features, but manual image analysis remains a labor and time-intensive process. Thus, this paper proposes a comprehensive pipeline for microscopy analysis that leverages the SegmentAnything to precisely demarcate individual organoids. Additionally, we introduce a set of morphological properties, including perimeter, area, radius, non-smoothness, and non-circularity, allowing researchers to analyze the organoid structures quantitatively and automatically. To validate the effectiveness of our approach, we conducted tests on bright-field images of human induced pluripotent stem cells (iPSCs) derived neural-epithelial (NE) organoids. The results obtained from our automatic pipeline closely align with manual organoid detection and measurement, showcasing the capability of our proposed method in accelerating organoids morphology analysis.Comment: submitted to SPIE: Medical Imaging 202

Analisis Pengaruh Perubahan Ukuran Cam Lobe Lift terhadap Performa pada Mesin Otto Empat Langkah

Perubahan ukuran lift pada cam lobe dipelajari untuk melihat pengaruhnya terhadap nilai efisiensi volumetris dan performa pada mesin Otto empat langkah satu silinder berkapasitas 150 cc. Pengujian dilakukan dengan membandingkan cam standar sebesar 7,36 mm dengan cam yang memiliki lift lebih tinggi, yaitu 8,24 mm dan 8,53 mm. Pengujian dilakukan dengan software ANSYS untuk mendapatkan efisiensi volumetris, sedangkan performa diukur menggunakan dinamometer. Dari perhitungan dan pengujian, kedua cam modifikasi yang digunakan berhasil meningkatkan efisiensi volumetris dan performa dari mesin. Cam dengan lift 8,53 mm meningkatkan efisiensi volumetris dari 78,51% menjadi 84,1% dan performa dari 15,36 WHP ke 15,76 WHP pada 9000 rpm jika dibandingkan dengan cam standar

Myofibroblast transdifferentiation of keratocytes results in slower migration and lower sensitivity to mesoscale curvatures

Functional tissue repair after injury or disease is governed by the regenerative or fibrotic response by cells within the tissue. In the case of corneal damage, keratocytes are a key cell type that determine the outcome of the remodeling response by either adapting to a fibroblast or myofibroblast phenotype. Although a growing body of literature indicates that geometrical cues in the environment can influence Myo(fibroblast) phenotype, there is a lack of knowledge on whether and how differentiated keratocyte phenotype is affected by the curved tissue geometry in the cornea. To address this gap, in this study we characterized the phenotype of fibroblastic and transforming growth factor β (TGFβ)-induced myofibroblastic keratocytes and studied their migration behavior on curved culture substrates with varying curvatures. Immunofluorescence staining and quantification of cell morphological parameters showed that, generally, fibroblastic keratocytes were more likely to elongate, whereas myofibroblastic keratocytes expressed more pronounced α smooth muscle actin (α-SMA) and actin stress fibers as well as more mature focal adhesions. Interestingly, keratocyte adhesion on convex structures was weak and unstable, whereas they adhered normally on flat and concave structures. On concave cylinders, fibroblastic keratocytes migrated faster and with higher persistence along the longitudinal direction compared to myofibroblastic keratocytes. Moreover, this behavior became more pronounced on smaller cylinders (i.e., higher curvatures). Taken together, both keratocyte phenotypes can sense and respond to the sign and magnitude of substrate curvatures, however, myofibroblastic keratocytes exhibit weaker curvature sensing and slower migration on curved substrates compared to fibroblastic keratocytes. These findings provide fundamental insights into keratocyte phenotype after injury, but also exemplify the potential of tuning the physical cell environments in tissue engineering settings to steer towards a favorable regeneration response