Finding the best tour for travelling salesman problem using artificial ecosystem optimization

This paper presents a new method based on the artificial ecosystem optimization (AEO) algorithm for finding the shortest tour of the travelling salesman problem (TSP). Wherein, AEO is a newly developed algorithm based on the idea of the energy flow of living organisms in the ecosystem consisting of production, consumption and decomposition mechanisms. In order to improve the efficiency of the AEO for the TSP problem, the 2-opt movement technique is equipped to enhance the quality of the solutions created by the AEO. The effectiveness of AEO for the TSP problem has been verified on four TSP instances consisting of the 14, 30, 48 and 52 cities. Based on the calculated results and the compared results with the previous methods, the proposed AEO method is one of the effective approaches for solving the TSP problem

Expression, purification and evaluation of recombinant L-asparaginase inmehthylotrophic yeast Pichia pastoris: Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast Pichia pastoris: Research article

L-asparaginase (EC 3.5.1.1), a therapeutic enzyme used in the treatment of childhood acute lymphoblastic leukemia (ALL). Hence, the goal of this work is study the expression and evaluation of hydrolysis activity of native sequence (X12746) encoding for L-asparaginase from Erwinia chrysanthemi NCPBB1125 in the popular expression system Pichia pastoris. The sequence of asn encoded for mature protein was expressed in P. pastoris SMD1168 and X33. SDS-PAGE analysis showed recombinant L-asparaginase was secreted efficiently. Stable and high hydrolysis activity of extracellular L-asparaginase in P. pastoris SMD1168 making it a potential candidate to produce recombinant protein. After purification, a specific band whose appearance approximately 45 kDa indicating the glycosylated protein with specific activity by 6.251 Umg-1 and about 3 folds purifications.L-asparaginase (EC 3.5.1.1), một loại enzyme được sử dụng trong điều trị bệng ung thư bạch cầu mãn tính ở trẻ em. Mục tiêu của nghiên cứu này là biểu hiện và đánh giá hoạt tính thủy phân của L-asparaginase mã hóa bởi đoạn gene (X12746) tương ứng từ Erwinia chrysanthemi NCPBB1125 được biểu hiện trong nấm men Pichia pastoris. Gene đã được cắt signal peptide và biểu hiện trong P. pastoris SMD1168 and X33. Qua phân tích kết quả điện di SDS-PAGE của môi trường sau lên men, L-asparaginase tái tổ hợp được tìm thấy trong dịch ngoại bào của P. pastoris. Với khả năng sản xuất protein có hoạt tính cao hơn so với chủng P. pastoris X33, SMD1168 được lựa chọn để biểu hiện L-asparaginase tái tổ hợp. Sau khi tinh sạch, sự xuất hiện của một băng có kích khối lượng phân tử xấp xỉ 45 kDa trên điện di SDS-PAGE cho thấy protein tái tổ hợp đã bị glycosyl hóa với hoạt tính riêng 6.251 Umg-1 và đạt độ sạch 3.471 lần

The Distribution of Microplastics in Beach Sand in Tien Giang Province and Vung Tau City, Vietnam

Microplastics threaten the ecosystem because of undesirable properties such as non-biodegradability, easy-to-absorb persistant organic compounds, etc. They are found worldwide in marine, fresh water and beach sand environments. In this study, microplastics in beach sand samples from two sites in Tien Giang province and two sites in Vung Tau city were investigated. The results showed that the microplastics amount was 0 to 295 pieces/kg dry sand and they mainly distributed near estuarine areas. Microplastics were more prevalent at bathing sites than non-bathing sites. In Tien Giang fragments were the most dominant among the three types of shapes (fragments, fibers, granules) at 60.2%. In Vung Tau granules were most prevalent at 71.7%. The composition of the plastics was confirmed by Fourier-transform infrared spectroscopy. It was revealed that PE, PP and PS were the main types of plastics found in the sampling sites.

MISO DC-DC Farmbot

Making use of renewable energy directly from the location of production requires converting source power into usable power. The specific scope of this project focuses on the DC to DC conversion within a user friendly universal farmbot system. Since renewable sources vary widely in voltage and current, a wide input-range DC to DC converter is desired. Physical isolation, long lifespan, and adverse weather requires safe and reliable final product specifications. The goal of a very wide customer base drives the need for a product that does not require tinkering to get working, but to be usable out of the box for a vast majority of energy sources. This project designs, purely through simulation due to COVID-19 pandemic, a Universal Input Module (UIM) DC-DC converter, which acts as the first step from the energy source to the usable power bus. UIMs can be connected in parallel to effectively make a Multiple Input Single Output (MISO) system. The main component of conversion is a 4-switch buck-boost controller. Input filtering, output filtering, parallel function, and two theoretical renewable inputs are incorporated to give the simulated converter as realistic of a function as possible. The selection process for all main components, surrounding components, and equivalent simulation circuits is included. With use of LTSpice, the simulation results meet the customer’s specifications

FATTY ACID COMPOSITION AND ANTIOXIDANT ACTIVITY OF CAMELLIA NINHII SEED OIL COLLECTED IN LAM DONG PROVINCE

The seed oil of Camellia ninhii was studied for the first time on its fatty acid composition by gas chromatography-mass spectrometry (GC-MS) and antioxidant activity by the DPPH method. The results show that unsaturated fatty acids account for the largest amount, especially oleic acid with 45.43% of the total sample analyzed. In addition, other fatty acids, palmitic, linoleic, pentadecanoic, and two aromatic acids, benzoic and cinnamic, were present. The sample of C. ninhii seed oil exhibited mild antioxidant activity against DPPH free radicals with IC50 = 0.94 mg/mL

A novel method for determining fixed running time in operating electric train tracking optimal speed profile

Tracking the optimal speed profile in electric train operation has been proposed as a potential solution for reducing energy consumption in electric train operation, at no cost to improve infrastructure of existing Metro lines as well. However, the optimal speed profile needs to meet fixed running time. Therefore, this paper focuses on a new method for determining the fixed running time complied with the scheduled timetable when trains track the optimal speed profile. The novel method to ensure the fixed running time is the numerical-analytical one. Calculating accelerating time ta, coasting time tc, braking time tb via values of holding speed vh, braking speed vb of optimal speed profile with the constraint condition: the running time equal to the demand time. The other hands, vh and vb are determined by solving nonlinear equations with constraint conditions. Additionally, changing running time suit for each operation stage of metro lines or lines starting to conduct schedules by the numerical-analytical method is quite easy. Simulation results obtained for two scenarios with data collected from electrified trains of Cat Linh-Ha Dong metro line, Vietnam show that running time complied with scheduled timetables, energy saving by tracking optimal speed profile for the entire route is up to 8.7%, if the running time is one second longer than original time, energy saving is about 11.96%

Study on some groups of microorganism during the forest rehabilitation process in Song Ma district, Son La province

The paper has made assessement on changes of some soil microorganism groups during the process of forest rehabilitation after shifting cultivation and after clear cutting in Song Ma district, Son La province. Sampling has been made in forest areas naturally regenerated for 4-6; 9-11; 14-16 and above 20 years after shifting cultivation and after clear cutting. The results have shown that the total aerobic bacteria, the total actinomycetes and the total microfungi have increased from the rehabilitation stage of 4-6 years (3.12×102; 1.23×103 and 8.05×102 CFU/g respectively in forest areas after shifting cultivation and 4.25×104; 2.32×103 and 7.32×103CFU/g respectively in forest areas after clear cutting) to the rehabilitation stage of ≥ 20 years (3.54×105; 2.23×104 and 2.51×104 CFU/g respectively in forest areas after shifting cultivation and 4.35×106; 2.72×105 and 5.46×105 CFU/g respectively in forest areas after clear cutting). The capacity in free nitrogen fixation, in cellulose decomposition, in phosphates decomposition, and in polysaccharide production of the aerobic bacteria, of actinomycetes, and of microfungi is lowest during the first forest rehabilitation stage (4-6 years), then it grows and reaches the highest value during the stage of≥20 years (5.60×103; 2.75×104; 2.10×103; 1.36×104 CFU/g respectively in forest areas after shifting cultivation and 5.24×103; 3.14×105; 4.27×104; 5.39×104 CFU/g respectively in forest areas after clear cutting)

RESEARCH TRENDS ON COMMUNITY-BASED TOURISM IN THE PERIOD 2013 - 2023

Community-based tourism (CBT) has been around since the 1970s and so far, has grown in popularity in most continents. This study systematically evaluates and generalizes theoretical and practical issues on CBT based on 87 related articles published in scientific journals under the Scopus system from 2013 to 2023 through the application of content analysis methods. The results also show that research in this area has different research areas and mainly uses qualitative methods. The literature review identified a number of key themes including: (1) benefits of CBT development, (2) community and stakeholder engagement, (3) advantages and barriers in CBT development, (4) community perceptions about CBT, and (5) sustainable CBT development. The article has analyzed research trends on CBT: theory and application.  Article visualizations