Space Biosciences Division

In the Space Biosciences Division at NASA's Ames Research Center, we perform the biological research and technology development necessary to tackle the challenges of living in the extreme environments of space and to enable NASA's long-term human exploration mission. This brochure provides a broad overview for our research and development capabilities, several case study examples, and finally real-world applications and collaborative partnerships

Risk of cervical cancer is not increased in Chinese carrying homozygous arginine at codon 72 of p53

Homozygous arginine at codon 72 (HA72) of p53 was found in 22% of normal cervices and 30.0% of cervical cancers and no significant difference was detected between normal and cervical cancer with or without HPV 16/18. There was no correlation between HA72 and risk of cervical cancer in Chinese.link_to_subscribed_fulltex

Scalar decay in a three-dimensional chaotic flow

The decay of a passive scalar in a three-dimensional chaotic flow is studied using high-resolution numerical simulations. The (volume-preserving) flow considered is a three-dimensional extension of the randomised alternating sine flow employed extensively in studies of mixing in two dimensions. It is used to show that theoretical predictions for two-dimensional flows with small diffusivity carry over to three dimensions even though the stretching properties differ significantly. The variance decay rate, scalar field structure, and time evolution of statistical moments confirm that there are two distinct regimes of scalar decay: a locally controlled regime, which applies when the domain size is comparable to the characteristic lengthscale of the velocity field, and a globally controlled regime, which when applies when the domain is larger. Asymptotic predictions for the variance decay rate in both regimes show excellent agreement with the numerical results. Consideration of both the forward flow and its time reverse makes it possible to compare the scalar evolution in flows with one or two expanding directions; simulations confirm the theoretical prediction that the decay rate of the scalar is the same in both flows, despite the very different scalar field structures

Disparate Effects of Cu and V on Structures of Exohedral Transition Metal-Doped Silicon Clusters: A Combined Far-Infrared Spectroscopic and Computational Study

The growth mechanisms of small cationic silicon clusters containing up to 11 Si atoms, exohedrally doped by V and Cu atoms, are described. We find that as dopants, V and Cu follow two different paths: while V prefers substitution of a silicon atom in a highly coordinated position of the cationic bare silicon clusters, Cu favors adsorption to the neutral or cationic bare clusters in a lower coordination site. The different behavior of the two transition metals becomes evident in the structures of SinM+ (n = 4−11 for M = V, and n = 6−11 for M = Cu), which are investigated by density functional theory and, for several sizes, confirmed by comparison with their experimental vibrational spectra. The spectra are measured on the corresponding SinM+·Ar complexes, which can be formed for the exohedrally doped silicon clusters. The comparison between experimental and calculated spectra indicates that the BP86 functional is suitable to predict far-infrared spectra of these clusters. In most cases, the calculated infrared spectrum of the lowest-lying isomer fits well with the experiment, even when various isomers and different electronic states are close in energy. However, in a few cases, namely Si9Cu+, Si11Cu+, and Si10V+, the experimentally verified isomers are not the lowest in energy according to the density functional theory calculations, but their structures still follow the described growth mechanism. The different growth patterns of the two series of doped Si clusters reflect the role of the transition metal’s 3d orbitals in the binding of the dopant atoms

The Сategory of Nationality of Literature as a Subject of Comprehension in the History of Russian Criticism of the 19th Century

The article examines the history of the formation of the concept of “nationality” as an aesthetic category in Russian criticism of the 19th century. The research attempts to identify certain constants in understanding nationality as an ethnic-social concept and aesthetic category in fiction and to trace its transformations in changing historical conditions concerning certain ideological discourses and cultural paradigms. The works of V. G. Belinsky, A. S. Khomyakov, N. A. Dobrolyubov, I. V. Kireevsky, N. A. Berdyaev, N. K. Mikhailovsky, the correspondence of P. A. Vyazemsky and articles by A. S. Pushkin demonstrates some examples of understanding nationality. Despite the variety of approaches to the interpretation of nationality in later times, the most adequate remains the thoughts contained in the works of Belinsky and the Slavophiles

Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose.

BackgroundUnderstanding how fungi degrade lignocellulose is a cornerstone of improving renewables-based biotechnology, in particular for the production of hydrolytic enzymes. Considerable progress has been made in investigating fungal degradation during time-points where CAZyme expression peaks. However, a robust understanding of the fungal survival strategies over its life time on lignocellulose is thereby missed. Here we aimed to uncover the physiological responses of the biotechnological workhorse and enzyme producer Aspergillus niger over its life time to six substrates important for biofuel production.ResultsWe analysed the response of A. niger to the feedstock Miscanthus and compared it with our previous study on wheat straw, alone or in combination with hydrothermal or ionic liquid feedstock pretreatments. Conserved (substrate-independent) metabolic responses as well as those affected by pretreatment and feedstock were identified via multivariate analysis of genome-wide transcriptomics combined with targeted transcript and protein analyses and mapping to a metabolic model. Initial exposure to all substrates increased fatty acid beta-oxidation and lipid metabolism transcripts. In a strain carrying a deletion of the ortholog of the Aspergillus nidulans fatty acid beta-oxidation transcriptional regulator farA, there was a reduction in expression of selected lignocellulose degradative CAZyme-encoding genes suggesting that beta-oxidation contributes to adaptation to lignocellulose. Mannan degradation expression was wheat straw feedstock-dependent and pectin degradation was higher on the untreated substrates. In the later life stages, known and novel secondary metabolite gene clusters were activated, which are of high interest due to their potential to synthesize bioactive compounds.ConclusionIn this study, which includes the first transcriptional response of Aspergilli to Miscanthus, we highlighted that life time as well as substrate composition and structure (via variations in pretreatment and feedstock) influence the fungal responses to lignocellulose. We also demonstrated that the fungal response contains physiological stages that are conserved across substrates and are typically found outside of the conditions with high CAZyme expression, as exemplified by the stages that are dominated by lipid and secondary metabolism