ECONOMICS OF THE FROZEN FOOD DISTRIBUTION SYSTEM

Agribusiness,

Determining the Effect of a CBC-based Oxime on Cell Viability and Apoptosis in Sea Urchin Embryos

Natural products are an important source of existing and potential new anti-cancer drugs. We have previously shown that cannabichromene (CBC) oxime and several CBC oxime esters have potent anti-mitotic activity when used in bioassays with sea urchin embryos. In this study we explored the effect of the CBC oxime on cell viability and apoptosis in sea urchin embryos. Newly fertilized or gastrula stage embryos were combined with 10µM or 50uM CBC oxime as well as a DMSO only control. At various timepoints, samples of the embryos were removed from the cultures and stained with three different fluorescent dyes. NucBlue (ThermoFisher) was used to determine cell number, apoptotic effects were observed with CellEvent caspace3/7 (ThermoFisher), and cell viability was determined with Live-or-Dye (Biotium). As expected, there was a clear effect of the CBC oxime on the rate of cell division. The embryos exposed to both 10uM and 50uM CBC oxime showed a reduction in cell division compared to the controls, with an almost total inhibition in the higher concentration. Cell viability assays using the vital stain Live-or-Dye showed a concentration dependent effect of the CBC oxime on cell viability, with levels of cell death reaching almost 80% in the 50uM concentration by 3 hours after drug addition. Finally, staining with CellEvent caspace3/7, which indicates the presence of active caspase activity, demonstrated concentration dependent induction of apoptosis following exposure to the CBC oxime drug. Therefore, the loss of cell viability observed was due to apoptosis rather than necrosis. There are clear toxic effects seen when this drug is exposed to developing sea urchin embryos, suggesting that it might have potential use as a chemotherapy agent capable of inducing apoptosis in cancer cells. We plan to test this hypothesis on human cancer cell lines in the near future

An Ecological Study of the Lagoons Surrounding the John F. Kennedy Space Center, Brevard County, Florida

The studies reported here are the result of a three year effort to define the major biological, microbiological, chemical and geological characteristics of the water of the Indian River lagoon around the Kennedy Space Center and to determine the movements of those waters within and between the various basins. This work was the result of a jointly funded agreement between the Florida Institute of Technology and John F. Kennedy Space Center, NASA under NASA Grant NGR 10-015-008, dated April 11, 1972. This cost sharing grant was renewed for each of two successive years. Sampling operations were terminated August 31, 1975

Minutes of Marti School Board of Trustees September 7, 1960

Minutes from the Board of Trustees of The Marti School discussing status of the school, the addition of two new board members, the arrival of new faculty, and more.https://corescholar.libraries.wright.edu/special_ms358_documents/1022/thumbnail.jp

When The Flag Goes By

https://digitalcommons.library.umaine.edu/mmb-vp/2716/thumbnail.jp

Context based clearing procedure: A niching method for genetic algorithms

AbstractIn this paper we present CBC (context based clearing), a procedure for solving the niching problem. CBC is a clearing technique governed by the amount of heterogeneity in a subpopulation as measured by the standard deviation. CBC was tested using the M7 function, a massively multimodal deceptive optimization function typically used for testing the efficiency of finding global optima in a search space. The results are compared with a standard clearing procedure. Results show that CBC reaches global optima several generations earlier than in the standard clearing procedure. In this work the target was to test the effectiveness of context information in controlling clearing. A subpopulation includes a fixed number of candidates rather than a fixed radius. Each subpopulation is then cleared either totally or partially according to the heterogeneity of its candidates. This automatically regulates the radius size of the area cleared around the pivot of the subpopulation

DiagHunter and GenoPix2D: programs for genomic comparisons, large-scale homology discovery and visualization

The DiagHunter and GenoPix2D applications work together to enable genomic comparisons and exploration at both genome-wide and single-gene scales. DiagHunter identifies homologous regions (synteny blocks) within or between genomes. DiagHunter works efficiently with diverse, large datasets to predict extended and interrupted synteny blocks and to generate graphical and text output quickly. GenoPix2D allows interactive display of synteny blocks and other genomic features, as well as querying by annotation and by sequence similarity

The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana

BACKGROUND: Most genes in Arabidopsis thaliana are members of gene families. How do the members of gene families arise, and how are gene family copy numbers maintained? Some gene families may evolve primarily through tandem duplication and high rates of birth and death in clusters, and others through infrequent polyploidy or large-scale segmental duplications and subsequent losses. RESULTS: Our approach to understanding the mechanisms of gene family evolution was to construct phylogenies for 50 large gene families in Arabidopsis thaliana, identify large internal segmental duplications in Arabidopsis, map gene duplications onto the segmental duplications, and use this information to identify which nodes in each phylogeny arose due to segmental or tandem duplication. Examples of six gene families exemplifying characteristic modes are described. Distributions of gene family sizes and patterns of duplication by genomic distance are also described in order to characterize patterns of local duplication and copy number for large gene families. Both gene family size and duplication by distance closely follow power-law distributions. CONCLUSIONS: Combining information about genomic segmental duplications, gene family phylogenies, and gene positions provides a method to evaluate contributions of tandem duplication and segmental genome duplication in the generation and maintenance of gene families. These differences appear to correspond meaningfully to differences in functional roles of the members of the gene families