418 research outputs found
Health-related quality of life and survival of cancer patients admitted to ICUs: Results of the QALY study
Brazilian Guidelines for Hereditary Angioedema Management - 2017 Update Part 1: Definition, Classification and Diagnosis
Hereditary angioedema is an autosomal dominant disease characterized by recurrent angioedema attacks with the involvement of multiple organs. The disease is unknown to many health professionals and is therefore underdiagnosed. Patients who are not adequately diagnosed and treated have an estimated mortality rate ranging from 25% to 40% due to asphyxiation by laryngeal angioedema. Intestinal angioedema is another important and incapacitating presentation that may be the main or only manifestation during an attack. In this article, a group of experts from the âAssociação Brasileira de Alergia e Imunologia (ASBAI)â and the âGrupo de Estudos Brasileiro em Angioedema HereditĂĄrio (GEBRAEH)â has updated the Brazilian guidelines for the diagnosis and treatment of hereditary angioedema
Cardiac-Oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis
Trypanosoma cruzi elicits reactive oxygen species (ROS) of inflammatory and mitochondrial origin in infected hosts. In this study, we examined ROS-induced oxidative modifications in the heart and determined whether the resultant oxidized cardiac proteins are targets of immune response and of pathological significance in Chagas disease. Heart biopsies from chagasic mice, rats and human patients exhibited, when compared to those from normal controls, a substantial increase in protein 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), carbonyl, and 3-nitrotyrosine (3-NT) adducts. To evaluate whether oxidized proteins gain antigenic properties, heart homogenates or isolated cardiomyocytes were oxidized in vitro and one- or two-dimensional gel electrophoresis (2D-GE)/Western blotting (WB) was performed to investigate the proteomic oxidative changes and recognition of oxidized proteins by sera antibodies in chagasic rodents (mice, rats) and human patients. Human cardiomyocytes exhibited LD50 sensitivity to 30 ”M 4-HNE and 100 ”M H2O2 at 6 h and 12 h, respectively. In vitro oxidation with 4-HNE or H2O2 resulted in a substantial increase in 4-HNE- and carbonyl-modified proteins that correlated with increased recognition of cardiac (cardiomyocytes) proteins by sera antibodies of chagasic rodents and human patients. 2D-GE/Western blotting followed by MALDI-TOF-MS/MS analysis to identify cardiac proteins that were oxidized and recognized by human chagasic sera yielded 82 unique proteins. We validated the 2D-GE results by enzyme-linked immunosorbent assay (ELISA) and WB and demonstrated that oxidation of recombinant titin enhanced its immunogenicity and recognition by sera antibodies from chagasic hosts (rats and humans). Treatment of infected rats with phenyl-α-tert-butyl nitrone (PBN, antioxidant) resulted in normalized immune detection of cardiac proteins associated with control of cardiac pathology and preservation of heart contractile function in chagasic rats. We conclude that ROS-induced, cardiac-oxidized antigens are targets of immune recognition by antibodies and molecular determinants for pathogenesis during Chagas disease
Evapotranspiração e coeficientes de cultivo da beterraba orgĂąnica sob cobertura morta de leguminosa e gramĂnea.
As prĂĄticas agrĂcolas que maximizam a produtividade e o uso da ĂĄgua sĂŁo de vital importĂąncia para a agricultura. Assim, foram testados trĂȘs tipos de manejo do solo com objetivo de determinar a evapotranspiração (ETc) e os coeficientes de cultivo (kc) da beterraba. Os tipos de manejo foram a utilização de coberturas mortas vegetais, denominadas capim cameroon (Pennisetum purpureum), gliricĂdia (Gliricidia sepium) e solo sem cobertura morta em ĂĄrea experimental do SIPA (Sistema Integrado de Produção OrgĂąnica) localizado em SeropĂ©dica, Brasil. A lĂąmina de irrigação foi estimada com base no balanço de ĂĄgua no solo, cujo monitoramento foi realizado com a tĂ©cnica da TDR. As ETc acumuladas para a cultura da beterraba foram 59,41; 55,31 e 119,62 mm, respectivamente, para capim cameroon, gliricĂdia e solo sem cobertura morta. A evapotranspiração de referĂȘncia (ETo) foi obtida por meio do modelo de Penamn-Monteith. Os valores mĂ©dios de kc obtidos para as fases inicial, mĂ©dia e final de desenvolvimento foram de 0,39; 0,42 e 1,02; 0,79; 0,76 e 1,18; e 0,56; 0,61 e 0,84, respectivamente, para capim cameroon, gliricĂdia e solo sem cobertura morta. O uso da cobertura do solo com gramĂnea ou leguminosa minimizou de forma expressiva a demanda hĂdrica da cultura da beterraba (Beta vulgaris)
Shrimps from the Santana Group (Cretaceous: Albian): new species (Crustacea: Decapoda: Dendrobranchiata) and new record (Crustacea: Decapoda: Caridea)
Insulin-like growth factor (IgF)-I, IgF binding protein-3, and prostate cancer: correlation with gleason score
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