Studi Fisikokimia Betasianin dalam Kulit Buah Naga dan Aplikasinya sebagai Pewarna Merah Alami Sediaan Farmasi: Physicochemical Study Of Betacyanin From Dragon Fruit Rind And Its Application AS Natural Dye For Pharmaceutical Dosage Form

The rind of dragon fruit (Hylocereus lemairel (Hook.) Britton & Rose) is a waste material that has not been optimally utilized. The rind contains betacyanin pigment that has many benefits in pharmaceutical products. The study aimed to characterize the physicochemical properties, to evaluate the stability and to apply betacyanin in dragon fruit rind as a natural dye. The extraction process was done by using water as solvent which was sonicated at 50 kHz for 30 minutes at 25 °C. Extract was freeze dried for 48 hours. The dried extract was purified by using preparative TLC and physicochemically analyzed by using UV-Vis and FTIR spectrophotometer. The stability of betacyanin extract against pH and temperature was examined and applied as a dye in tablet imprinting. The result of this study indicated that betacyanin was found at Rf value of 0.6 same as the betacyanin standard. The maximum wavelength of betacyanin was obtained at 534 nm and the IR spectra showed similarity with betacyanin standard with the same functional groups between 4000-600 cm-1 although there was a slight shift in the wavenumber but it still in the range. The stability study were stable at temperature below 40 °C and at range pH 4-6. Betacyanin applications as natural dye of tablet have been successfully carried out with good color stability during 3 months of storage at room temperature

The Composition of Species and Structure of Seagrass Fish Community in Tanjung Tiram – Inner Ambon Bay

The study was conducted in March - May 2011 in the coastal waters of Tanjung Tiram – inner Ambon bay. The aims of the study were to determine the composition of species and structure of fish communities in seagrass beds ecosystems. Fish were collected every spring and neap tide for three month periode with a swept area method using beach seine. Fishes were collected as many as 6444 individuals representing 68 species from 29 families. Siganus canaliculatus was contributed up to 62.91% of the total individual fish found.The fish community structure was varied between spring and neap tide. Index of dominance was in low category, diversity in medium, and evenness in unstable conditions. Moreover, the results indicated that seagrass ecosystems in Tanjung Tiram (TAD) have an important role as spawning, nursery ground, and feeding ground. Therefore, management and conservation efforts are urgently needed to maintain the ecological role of seagrass ecosystems for the sustainability of the fish resources

Pemetaan Spasio-temporal Ikan-ikan Herbivora di Kepulauan Spermonde, Sulawesi Selatan [Spatio-temporal Mapping Of Herbivorous Fishes At Spermonde Islands, South Sulawesi]

Ikan herbivora di terumbu karang menjadi salah satu indikator tingkat kesehatan ekosistem. Pada terumbu karang yang sehat biasanya ditandai dengan kelimpahan ikan herbivora yang tinggi. Penelitian ini bertujuan untuk memetakan distribusi spasial dan temporal ikan herbivora dalam kaitannya dengan kondisi kesehatan karang. Pendataan jumlah jenis dan kepadatan ikan herbivora dengan metode transek sabuk dan pencacahan dengan teknik visual sensus, sedangkan penilaian kesehatan karang dengan metode transek kuadran. Data sebaran diplot dengan teknik pemetaan sedangkan data kepadatan dikelompokkan berdasarkan pulau dan dianalisis perbedaannya dengan analisis ragam. Hubungan antara kepadatan ikan herbivora dengan kesehatan karang dilakukan dengan menggunakan regresi sederhana. Hasil penelitian menunjukkan rata-rata kepadatan ikan herbivora periode April-Agustus dengan kisaran 0,014-0,532 ekor.m-2. Hasil analisis ragam menunjukkan bahwa ada perbedaan kepadatan ikan herbivora antarpulau. Hubungan antara kepadatan ikan herbivora dengan kondisi kesehatan karang berkorelasi positif dan nyat

ANALISIS SENYAWA BERBAHAYA PARFUM ISI ULANG YANG DIJUAL DI KOTA PADANG MENGGUNAKAN METODE KROMATROGRAFI GAS-SPEKTROMETRI MASSA

Perfume is widely used by most of people in this world. The increasing demand of perfume has caused many producers cheating by adding dangerous compounds and also unregistered by the National Agency of Drug and Food Control Indonesia. The aim of this study is to analyze the dangerous compounds in unregistered perfumes sold in Pasar Raya Padang City by using gas chromatography-mass spectrometry (GC-MS) method. In this Study, samples (A, B, C, D and E) were collected and the value of specific weight and refractive index of the samples were analyzed. The results showed that five samples contained relatively similar chemical compounds. Twelve compounds were detected and seven of them were harmful to health in refill perfumes which were dipropylene glycol, linalool, lily aldehyde, benzenepentanol, dimethylbenzyl carbinyl acetate, dihydro methyl jasmonate, alpha hexyl cinnamic aldehyde, based on Material Safety Data Sheet (MSDS). Regular monitoring of chemicals used in the manufacture of perfumes which may cause health risks to users should be controlled by National Agency of Drug and Food Control Indonesia

Aplikasi Metode Multycriteria Decision Making (MCDM)dengan Teknik Pembobotan Dalam Mengidentifikasi Dan Mendesain Kawasan Konservasi Perairan Daerah Di Kabupaten Luwu Utara, Provinsi Sulawesi Selatan

The study, in 2012, has successfully formulated with the MCDM for each allocation of space in KKPD allotment (core areas, sustainable fisheries zone, used zone, and other zones). This weighting techniques need to be tested and be implemented in identifying and designing the KKPD in the study area. This study aims to identify and map the biophysical conditions and the potential of coastal and marine natural resources in marine conservation area candidate, North Luwu Regency; to identify the areas suitable for the KKPD based on weighting technique with the MCDM method; and to evaluate potential candidates for marine protected areas in the coastal region. This study used a survey method to perform in situ measurements of physico-chemical parameters, conducted a survey of coastal ecosystems using the transect method. The socio-economic data of coastal communities were collected using the questionnaire. The biophysical conditions and marine resources were analyzed using descriptive statistical methods. The results showed that the candidate region has a rich diversity of coastal ecosystems, but the ecosystem, particularly seagrass beds and coral reefs have been in damaged category. Only the mangrove ecosystem that was still in a good condition category with moderate-to-heavy levels of density. There were 6 species of seagrasses and 6 species of mangroves and 71 species of reef fish. It was discovered 2 regions corresponding to the allotment of the Core Zone, which is in the Region I and III with the total area of 654.22 hectares. For sustainable fisheries zone, Region II and IV would be the first choice with the total area of 620.27 hectares. The Used Zone was identified in the Region V with total area of 480.66 hectares. The total area of the region was equal to 1755.15 hectares. Marine protected areas of was suggested to the protection of coastal ecosystems including mangroves, seagrass beds, and coral reefs and its associated biota, especially the protection of local feeding ground of several species (sea turtles and dugongs)

Human papillomavirus genotype distribution among colposcopy diagnosed cervical precancerous lesions

Background: Cervical cancer (CC) is one of the primary causes of gynaecological cancer death. Cervical cancer is the fourth most frequent cancer worldwide, and it is the second most common cancer in Bangladesh. The stage of cervical cancer at diagnosis has a significant impact on survival. Cervical cancer mortality is high in Bangladesh due to late detection and limited management facilities. The aim of the study was to determine the pattern of human papillomavirus (HPV) genotype among colposcopy diagnosed cervical precancerous lesions. Methods: This cross-sectional study was conducted in the department of gynecological oncology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka. Total of 142 women attending the colposcopy clinic of BSMMU. Results: The mean age was found 38.7±7.3 years with a range from 30 to 60 years. 10 (7.0%) patients were found HPV 16 positive followed by 1 (0.7%) HPV 18, another hr-HPV 3 (2.1%), HPV 16 and other hr-HPV 3 (2.1%) and HPV 16, HPV 18 and other hr-HPV 1 (0.7%). Regarding colposcopy reports 99 (69.7%) patients had CIN I, 33 (23.7%) had CIN II and 10 (7.0%) had CIN III identification by colposcopy reports. 61 (43.0%) patients had CIN I followed by 15 (10.6%) had CIN II, 11 (7.7%) had CIN III, 7 (4.9%) had CIS, and 48 (33.8%) had normal or squamous metaplasia by histopathological reports. Conclusions: It can be concluded that among all the 14 hr-HPV genotype HPV 16 is more prevalent while HPV18 prevalence was very low in colposcopy diagnosed cervical precancer cases. The study revealed HPV16 was more common among high grade lesions

Emotional Biases and Recurrence in Major Depressive Disorder. Results of 2.5 Years Follow-Up of Drug-Free Cohort Vulnerable for Recurrence

An interesting factor explaining recurrence risk in Major Depressive Disorder (MDD) may be neuropsychological functioning, i.e., processing of emotional stimuli/information. Negatively biased processing of emotional stimuli/information has been found in both acute and (inconclusively) remitted states of MDD, and may be causally related to recurrence of depression. We aimed to investigate self-referent, memory and interpretation biases in recurrently depressed patients in remission and relate these biases to recurrence. We included 69 remitted recurrent MDD-patients (rrMDD-patients), 35–65 years, with ≥2 episodes, voluntarily free of antidepressant maintenance therapy for at least 4 weeks. We tested self-referent biases with an emotional categorization task, bias in emotional memory by free recall of the emotion categorization task 15 min after completing it, and interpretation bias with a facial expression recognition task. We compared these participants with 43 never-depressed controls matched for age, sex and intelligence. We followed the rrMDD-patients for 2.5 years and assessed recurrent depressive episodes by structured interview. The rrMDD-patients showed biases toward emotionally negative stimuli, faster responses to negative self-relevant characteristics in the emotional categorization, better recognition of sad faces, worse recognition of neutral faces with more misclassifications as angry or disgusting faces and less misclassifications as neutral faces (0.001 < p < 0.05). Of these, the number of misclassifications as angry and the overall performance in the emotional memory task were significantly associated with the time to recurrence (p ≤ 0.04), independent of residual symptoms and number of previous episodes. In a support vector machine data-driven model, prediction of recurrence-status could best be achieved (relative to observed recurrence-rate) with demographic and childhood adversity parameters (accuracy 78.1%; 1-sided p = 0.002); neuropsychological tests could not improve this prediction. Our data suggests a persisting (mood-incongruent) emotional bias when patients with recurrent depression are in remission. Moreover, these persisting biases might be mechanistically important for recurrence and prevention thereof