Colors For Wool Yarn

Colors for Wool Yarn is a manual used by the Du Pont Company to mix dyes for wool yarn. This book contains comprehensive information regarding the range of du Pont dyes suitable for application on wool in all stages of manufacture. Dyeings of both acid and chrome colors are shown on wool yarn. In addition, general dyeing instructions have been incorporated as well as a tabulation embracing most of the working and fastness properties. The following pages also contain specific information on the dyeing properties of the individual colors --Introduction, page 3.https://digitalcommons.winthrop.edu/rarebooks/1159/thumbnail.jp

Étude in vivo de la relation entre la structure et la fonction de la boucle variable de l'ARN de transfert de la sélénocystéine d'E. coli

La sélénocystéine est le 21e acide aminé encodé génétiquement et on la retrouve à travers les trois domaines de la vie. Elle est synthétisée sur l'ARNtSec par un processus unique. L'ARNtSec se distingue également au niveau structural. La tige acceptrice possède 8 (procaryotes) et 9 (eucaryotes) paires de bases, contrairement aux ARNt canoniques qui ont invariablement 7 paires de bases dans la tige acceptrice. De plus, la tige D a 2 paires de bases additionnelles qui remplacent les interactions tertiaires universelles 8-14, 15-48 qui sont absentes chez l'ARNtSec. D'autre part, la longueur de la boucle variable de l'ARNtSec est plus longue que la majorité des ARNt de type II. Dans ce mémoire, on se concentre sur la région de la boucle variable de l'ARNtSec . La recherche consiste à distinguer les paires de bases de la boucle variable qui sont essentielles à la biosynthèse et l’insertion de la sélénocystéine. De plus, on regarde si la paire de base additionnelle de la tige acceptrice de l'ARNtSec (procaryote) est essentielle pour l'insertion de la sélénocystéine. Pour répondre à ces questions, on a utilisé l'approche expérimentale Évolution Instantanée qui consiste au criblage in vivo d'ARNtSec fonctionnels chez E. coli. Dans ce travail, on montre que l'insertion de la sélénocystéine ne nécessite pas une spécificité de la longueur ou de la séquence de l'ARNtSec. On montre aussi que ni la longueur de la tige acceptrice ou du domaine tige acceptrice/tige T n'est essentielle pour avoir un ARNtSec fonctionnel.Selenocysteine is the 21st genetically encoded amino acid and is found across the three domains of life. It is synthesized on the tRNA-Sec by a single process. The tRNA-Sec also stands out at the structural level. The acceptor stem has 8 (bacteria) and 9 (eukaryote) base pairs, in contrast to canonical tRNA which invariably has 7 base pairs in the acceptor stem. Furthermore, the D-stem has two additional bases pairs which replace the universal tertiary interactions 8-14, 15-48 which are absent in the tRNA-Sec. On the other hand, the length of the extra-arm of tRNA-Sec is longer than the majority of type II tRNA . In this dissertation, we focus on the extra-arm of tRNA-Sec. The research consists of distinguishing the base pairs of the extra-arm that are essential for the biosynthesis and insertion of selenocysteine. In addition, we examine if the additional base pair in the acceptor stem (bacteria) is essential for the insertion of selenocysteine. To study these questions, we used the experimental Instant Evolution approach which consists of the in vivo screening of functional tRNA-Sec clones in E. coli. In this work, it is shown that the insertion of selenocysteine does not require a specific length or sequence of the tRNA-Sec. It is also shown that neither the length of the acceptor stem or of the acceptor/T domain is required to have a functional tRNA-Sec

STAT3 phosphorylation at serine 727 activates specific genetic programs and promotes clear cell renal cell carcinoma (ccRCC) aggressiveness

Cancer models; Molecular medicineModelos de cáncer; Medicina molecularModels de càncer; Medicina molecularThe signal transducer and activator of transcription 3 (STAT3) is a transcription factor mainly activated by phosphorylation in either tyrosine 705 (Y705) or serine 727 (S727) residues that regulates essential processes such as cell differentiation, apoptosis inhibition, or cell survival. Aberrant activation of STAT3 has been related to development of nearly 50% of human cancers including clear cell renal cell carcinoma (ccRCC). In fact, phosho-S727 (pS727) levels correlate with overall survival of ccRCC patients. With the aim to elucidate the contribution of STAT3 phosphorylation in ccRCC development and progression, we have generated human-derived ccRCC cell lines carrying STAT3 Y705 and S727 phosphomutants. Our data show that the phosphomimetic substitution Ser727Asp facilitates a pro-tumoral phenotype in vitro, in a Y705-phosphorylation-independent manner. Moreover, we describe that STAT3 phosphorylation state determines the expression of different subsets of target genes associated with distinct biological processes, being pS727-dependent genes the most related to cellular hallmarks of cancer. In summary, the present study constitutes the first analysis on the role of overall STAT3 phosphorylation state in ccRCC and demonstrates that pS727 promotes the expression of a specific subset of target genes that might be clinically relevant as novel biomarkers and potential therapeutic targets for ccRCC.This work was supported by Ministerio de Ciencia e Innovación (Grant No. SAF201459945-R and SAF2017-89989-R) to A.M.; American Association for Cancer Research (AACR, Ref #419589) to A.M. and Red de Investigación Renal REDinREN (Grant No. 12/0021/0013) to. A.M. The group holds the Quality Mention from the Generalitat de Catalunya (Grant No. 2021 SGR 01,600). J.A. was a recipient of the Ph.D. Fellow Program from Consejo Nacional de Ciencia y Tecnología (CONACyT), México (Grant No. 549678)

Identification of miRNAs and Their Target Genes Associated with Sunitinib Resistance in Clear Cell Renal Cell Carcinoma Patients

Sunitinib has greatly improved the survival of clear cell renal cell carcinoma (ccRCC) patients in recent years. However, 20–30% of treated patients do not respond. To identify miRNAs and genes associated with a response, comparisons were made between biopsies from responder and non-responder ccRCC patients. Using integrated transcriptomic analyses, we identified 37 miRNAs and 60 respective target genes, which were significantly associated with the NF-kappa B, PI3K-Akt and MAPK pathways. We validated expression of the miRNAs (miR-223, miR-155, miR-200b, miR-130b) and target genes (FLT1, PRDM1 and SAV1) in 35 ccRCC patients. High levels of miR-223 and low levels of FLT1, SAV1 and PRDM1 were associated with worse overall survival (OS), and combined miR-223 + SAV1 levels distinguished responders from non-responders (AUC = 0.92). Using immunohistochemical staining of 170 ccRCC patients, VEGFR1 (FLT1) expression was associated with treatment response, histological grade and RECIST (Response Evaluation Criteria in Solid Tumors) score, whereas SAV1 and BLIMP1 (PRDM1) were associated with metachronous metastatic disease. Using in situ hybridisation (ISH) to detect miR-155 we observed higher tumoural cell expression in non-responders, and non-tumoural cell expression with increased histological grade. In summary, our preliminary analysis using integrated miRNA-target gene analyses identified several novel biomarkers in ccRCC patients that surely warrant further investigation

Estudio de la identificación de los costos por carrera en el presupuesto anual de la unidad académica ciencias administrativas y comerciales de la UNEMI.

La implementación de metodologías de costos basados en actividades "ABC" para determinar los costos por carrera de la unidad académica ciencias administrativas y comerciales de la UNEMI, tiene como principal propósito identificar y analizar los costos que genera cada alumno por carrera que actualmente tiene la unidad; en su relación costo-beneficio con cifras que se obtienen mediante nuevas herramientas como representa el costeo basado en actividades(ABC), que sirve de apoyo para la toma adecuada y oportuna decisiones. Para lo cual se ha extendido una profunda información la misma que está distribuida por cincos capítulos que componen toda la tesis. Detallando prolijamente la problemática sus causas y efectos, delimitación, formulación sistematización y su correspondiente justificación y los objetivos del tema propuesto que beneficiará a la unidad académica ciencias administrativas y comerciales de la UNEMI, lo cual no cuenta con una metodología de costo que le representan por cada estudiante de las diferentes carreras de la unidad. En el capítulo 2 se establece los antecedentes históricos sobre la historia y conceptos de la contabilidad,, presupuesto y costos, para llegar a obtener la información necesaria nos enfocamos en la búsqueda por medio de libro, repositorios y documentos virtuales, esto nos ayuda a tener una mayor comprensión del trabajo investigación y se ha establecido la respectiva hipótesis y sus variables, en el siguiente capítulo se definió que tipo de metodología y técnicas vamos a utilizar para nuestra investigación. En el capítulo 4 se plasmaron los resultados de las encuestas realizadas al personal administrativo que tiene relación con la unidad, mediantes estos resultados se pudo corroborar la necesidad que existe en la unidad académica ciencias administrativas y comerciales de la UNEMI al no contar con un modelo de costos que identifiquen los costos que genera cada alumno de las carreras de la unidad