Impact of the superimposition reference area on intraoral scanning accuracy in a partially dentate maxilla

Statement of problem The alignment of 3-dimensional (3D) files involves selecting a reference area before performing a local best fit alignment during the digital scan superimposition and is essential for comparing digital scans. Scan alignment relies on both reference area location and the alignment algorithm. However, a consensus on the impact of different reference areas on intraoral scanning accuracy is lacking. Purpose The purpose of this in vitro study was to assess the impact of 3 superimposition reference areas on the accuracy of 3 intraoral scanners for a partially dentate maxilla. Material and methods A Kennedy class II resin cast was scanned using 3 intraoral scanners (Primescan, TRIOS 3, and Emerald) outputting 30 digital scans (10 per scanner). Test scans from intraoral scanners were subsequently compared with a reference digital standard tessellation language file generated by a laboratory scanner with validated accuracy. The files were superimposed using best fit alignment for each intraoral scanner using 3 different superimposition reference areas (whole region of interest, palate, and all teeth). Accuracy was assessed by using a 3D analysis program (Geomagic Control X; 3D systems) for each scanner at 4 preselected areas. Test and reference scan differences were depicted on color maps and quantified via root mean square deviations. Differences were analyzed using regression analysis with the post hoc student t test and Bonferroni correction (α=.05). Results The TRIOS 3 and Emerald produced positive deviations in the palatal color maps, whereas Primescan produced more uniform color maps, regardless of the superimposition strategy used. Primescan exhibited the best accuracy (trueness and precision) in both palatal and bounded edentulous areas, regardless of the superimposition reference area. The TRIOS 3 recorded the highest distal extension trueness (ranging from 42.9±7.7 µm to 65 ±19.5 µm), and Primescan achieved the highest precision (ranging from 28.5 ±9.8 µm to 48.9 ±16.9 µm), regardless of the superimposition area. Emerald demonstrated the highest teeth trueness (ranging from 31.6 ±6.8 µm to 69.6 ±11.5 µm), while Primescan produced the highest precision (ranging from 17.9 ±6.1 µm to 30.7 ±9.2 µm), regardless of the reference area used. Conclusions The chosen reference area for best fit alignment significantly influenced digital scan accuracy (P<.001). Primescan displayed the highest palatal and bounded edentulous area accuracy, with TRIOS 3 recording the highest distal extension trueness. Emerald recorded the highest teeth trueness and Primescan recorded the highest distal extension and tooth precision. All conclusions were independent of the superimposition strategy used

Potentiation of raloxifene cytotoxicity against MCF-7 breast cancer cell lines via transdermal delivery and loading on self-emulsifying nanoemulsions

Purpose: To enhance raloxifene (RLX) delivery and cytotoxicity against breast cancer (MCF-7) cell lines. Methods: This was a solubility study of RLX in different oils, surfactants, and co-surfactants. Twelve formulae were tested to reach the smallest&nbsp; globular size, and hydroxypropyl methylcellulose, (HPMC), and Carbopol 947 polymers were tested for formation of transdermal films. The formula&nbsp; with the lowest size was compared with raw RLX in diffusion studies using a Franz diffusion cell. Finally, a cytotoxicity study against MCF-7 breast cancer cell lines was conducted. Results: The maximum solubility of RLX was in Tween 80, peppermint oil, and PEG 200; therefore, these were the main components of the 12 formulations. The release of RLX loaded on the selfnanoemulsion drug delivery system (SNEDDS) was increased 3-fold compared with raw RLX.Cytotoxicity results revealed that RLX SNEDDs decreased MCF-7 cell survival by approximately 40 %, compared with raw RLX (control), which augmented the RLX suppression of breast cancer cell lines. Conclusion: Improvement in RLX cytotoxicity is a novel strategy to suppress breast cancer. Keywords: Raloxifene, Osteoporosis, Bioavailability, Nanoemulsion, Nanoparticle

Hepatitis C virus as possible etiologic factor in idiopathic nephrotic syndrome among Egyptian patients

Hepatitis C virus (HCY) infection is associated with a variety of extrahepatic disorders. including cryoglobulinaernia and glomerulonephritis. Hepatitis C virus (HCV) glornerulopathy may be present as a primary glomerular disease. Our study included 50 adult Egyptian patients who were diagnosed as idiopathic nephrotic syndrome (NS). We described the clinical, pathological and immunological features of these patients. There was a high prevalence (50%) of HCV infection among these patients. The studied risk factors included history of; blood transfusion (16%) operation (24%) or antibilharzial drugs (76%). Hepatomegaly was observed in 24% of cases.Mernbranoproliferative glomerulonephritis (MPGN) was the commonest pathological  type associated with HCV (48%). Other patterns included focal segmental glomerulosclerosis (FSGS) in 32%, membranous in 8% and minimal change glomerulonephritis in 12%. Cryoglobulins were detected in 5.6% of 18 patients with HCV and idiopathic NS.Patients having HCV infection and membranoproliferative glomerulonephritis had hypocomplementemia and antinuclear antibodies were detected in 41.6%.Realising that HCV infection may be linked to different glomerulopathies, thus routine screening for HCV should be considered in serologic work-up of patients with glomerulopathy. Nevertheless, seroepiderniological studies including larger number of patients with glornerulopathy are therefore necessary to specify its relation with HCV infection

The theory of a "staphylococcus superantigen" in chronic rhinosinusitis with nasal polyps: myth or reality?

OBJECTIVE: The aim of our study was to search for evidence of a "staphylococcus superantigen" in chronic rhinosinusitis with nasal polyps. PATIENTS AND METHODS: Sixty-nine patients with chronic rhinosinusitis with nasal polyps and 45 healthy controls were included in the study. All patients in the study and control groups underwent bacteriological and immunological examination on nasal smear samples. Total IgE and the following cytokines were tested in all patients: tumor necrosis factor (TNF), interleukin-1 (IL1), interleukin-6 (IL6), interleukin-8 (IL8). RESULTS: The concentration of bacteria in the nasal cavity was much higher in patients in the study group compared to those in the control group, mainly due to staphylococci. In species identification of staphylococci, bacteria most represented were S. aureus and S. epidermidis. The greater the concentration of S. aureus, the lower the level of IgE. Proinflam-matory cytokines were uniformly increased in patients with nasal polyps. The level of IgE was maximal in patients with chronic rhinosinusitis with nasal polyps with a poor growth of culture and minimal in patients with abundant growth, suggesting that in the latter the effect of eosinophilic inflammation on the disease was reduced, and conversely, the activity of eosinophilic inflammation was maximal with a poor seeding of the nasal cavity. CONCLUSIONS: Although this study has some limits, our findings do not support the theory of a staphylococcus superantigen in which the IgE level and eosinophilic inflammation should increase with increasing activity of Staphylococcus aureus. Further research supported by a larger sample of patients is required to better delineate the role of a staphylococcus superantigen in the pathogenesis of patients with chronic rhinosinusitis with nasal polyps

A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection

We provide a detailed overview of a novel high-throughput protein microarray assay for the determination of anti-C. difficile antibody levels in human sera and in separate preparations of polyclonal IVIg. The protocol describes the methodological steps involved in sample preparation, printing of arrays, assay procedure and data analysis. In addition, this protocol could be further developed to incorporate diverse clinical samples including plasma and cell culture supernatants. Herein, a combination of isotype (IgG, IgA IgM), subclass (IgG1, IgG2, IgG3, IgG4, IgA1, IgA2), and strain-specific antibodies to highly purified whole C. difficile toxins A and B (toxinotype 0, strain VPI 10463, ribotype 087), toxin B from a C. difficile toxin-B-only expressing strain (CCUG 20309), precursor form of B fragment of binary toxin, pCDTb, ribotype-specific whole surface layer proteins (SLPs; 001, 002, 027) and control proteins (Tetanus toxoid and Candida albicans) were determined by protein microarray. Microarrays were probed with sera from individuals with C. difficile infection (CDI), cystic fibrosis (CF) without diarrhea, healthy controls and individuals pre- and post-IVIg therapy for treatment of CDI, combined immunodeficiency disorder and chronic inflammatory demyelinating polyradiculopathy. Significant differences in toxin neutralization efficacies and multi-isotype specific antibody levels were seen between patient groups, commercial preparations of IVIg and sera before and following IVIg administration. A significant correlation was observed between microarray and enzyme-linked immunosorbent assay (ELISA) for antitoxin IgG levels in serum samples. These results suggest that microarray could become a promising tool for profiling antibody responses to C. difficile antigens in vaccinated or infected humans. With further refinement of antigen panels and a reduction in production costs, it is anticipated that microarray technology may help optimize and select the most clinically useful immunotherapies for C. difficile infection in a patient-specific manner

Investigation of sediment accumulation in Nubia Lake, using RS/GIS

The main focus of this paper is to detect the changes in AHDL bed surface (sedimentation and erosion) in the period 2000-2012 in the active sedimentation zone of the Lake which is located in Nubia Lake (Sudanese part of AHDL). To enable this detection, the remote sensing (RS) and (Geographic Information Systems) GIS techniques are used to build the 3-D profile of the Lake portion where most of the sediment accumulate. Also, the accumulated sediment during this period is computed using the developed 3-D profile and using the cross section method as conducted by Aswan High Dam Authority (AHDA). Also, the surface areas of sedimentation and erosion are mapped and computed. Results indicated that sedimentation are dominant in years with high flood while erosion occurs when the incoming flow to the lake is low. Moreover, results indicate that the present approach overestimate the sedimentation amount by about 4% compared to the results of the method used by AHDA

A stand-alone Zero-Liquid-Discharge greenhouse model with rainwater harvesting capability

This is the author accepted manuscriptGlobal warming is a prevalent topic throughout the world. The IPCC predicts that the maximum potential global temperature increase will be 4.8 oC by 2100. It has been concluded that a temperature rise of 1.4 oC or higher will have statistically significant impacts on global precipitation levels. Therefore, there is a need to investigate the future trends of precipitation and subsequent irrigation methods. This study will discuss a new multi-functional zero liquid discharge (ZLD) system for a greenhouse, incorporating a humidification dehumidification (HDH) mechanism, solar still desalination and rainwater harvesting. The focus of this paper is on analysing the water production of the system. Although previous literature discusses the inefficiency of solar still (SS) desalination, the fresh water produced during similar experiments has shown otherwise, desalinating 0.95 L/m²/hr of saline water. Using multiple panels could therefore give a substantial output of distilled water for certain usage such as agriculture. Implementing solar stills of large surface area would also allow the collection of rainwater thus increasing the total water productivity of the system. The ZLD system aims to produce no waste product and use the output brine water for aquaculture and salt cultivation.British CouncilScience & Technology Development Fund (STDF), Egyp