Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils

In this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert’s experimental design applied the determination of the optimal operating conditions. An investigation into the effects of the methanol composition (50–100%), the volume of eluent (1–12 mL), and pH (1–3) on the extraction of phenols acids and total phenols from Tunisian olive oils was performed. The results showed that the extraction conditions had a significant effect on the extraction efficiency. The experiment showed that the greatest conditions for the SPE of phenolic acids were the methanol composition at 90.3%, pH at 2.9, and volume at 7.5 mL, respectively. The optimal conditions were applied to different types of olive oils, and it could be concluded that larger concentrations of polyphenols were found in extra virgin olive oil (89.15–218), whereas the lowest levels of these compounds (66.8 and 5.1) were found in cold-pressed crude olive oil and olive pomace oil, respectively

Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils

In this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert’s experimental design applied the determination of the optimal operating conditions. An investigation into the effects of the methanol composition (50–100%), the volume of eluent (1–12 mL), and pH (1–3) on the extraction of phenols acids and total phenols from Tunisian olive oils was performed. The results showed that the extraction conditions had a significant effect on the extraction efficiency. The experiment showed that the greatest conditions for the SPE of phenolic acids were the methanol composition at 90.3%, pH at 2.9, and volume at 7.5 mL, respectively. The optimal conditions were applied to different types of olive oils, and it could be concluded that larger concentrations of polyphenols were found in extra virgin olive oil (89.15–218), whereas the lowest levels of these compounds (66.8 and 5.1) were found in cold-pressed crude olive oil and olive pomace oil, respectively

In Vitro Antioxidant, Antibacterial and Mechanisms of Action of Ethanolic Extracts of Five Tunisian Plants against Bacteria

Pistacia lentiscus, Rosmarinus officinalis, Erica multiflora, Calicotome villosa, and Phillyrea latifolia were considered important medicinal herbs and were used to treat various ailments. The present study was designed to evaluate the antioxidant and antimicrobial activities of ethanolic extracts (EEs). P. lentiscus and R. officinalis were the richest species in phenolic compounds. Similarly, both species showed the highest values of flavonoids. While the EEs of P. lentiscus, E. multiflora, and C. villosa had higher amounts of tannins. These phenolic compounds were evaluated by two different tests, namely diphenyl picrylhydrazyl (DPPH) and ferric iron-reducing power (FRAP). The IC50 values were found to be significant (p < 0.05) for P. lentiscus and E. multiflora. Similarly, both plants showed the highest ferric-reducing antioxidant power (FRAP). This study has been conducted to evaluate the antibacterial potential of EEs against selected bacteria—Gram-positive bacteria (Staphylococcus aureus ATCC 29213, Listeria monocytogenes ATCC 7644) and Gram-negative bacteria (Escherichia coli ATCC 8739, Salmonella typhimurium NCTC 6017)—and determine their modes of action. The ethanolic extracts inhibited bacterial growth by producing concentration-dependent zones of inhibition. Treatment with these extracts at their minimum inhibitory concentrations (MICs) showed a significant reduction (p < 0.05) in the viability of bacteria. The extracts did not induce total lysis. Bacteria organisms treated with EEs at MICs showed a significant (p < 0.05) loss of tolerance to NaCl (5%). Our results highlighted the use of plant extracts as natural antibacterials that can be safely used in health care and led to the understanding of the antibacterial mechanism of plant extracts

Solid-Phase extraction of polycyclic aromatic hydrocarbons from water samples using CTAB-TIO2 modified nanotubes

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Nanostructured Titanium Dioxide (NS-TiO₂)

During the past decade, research in the area of synthesis and applications of nanostructured titanium dioxide (NS TiO2) has become tremendous. NS TiO2 materials have shown great potential and a wide range of applications. The decrease in the particle size and the increase of the surface/volume ratio lead to the increase of the specific surface and the modification of the physicochemical properties and the appearance of new interesting properties (photocatalytic, optical, magnetic, electronic...). Their new morphology even allows the appearance of new biological properties. NS TiO2 can thus be used for the same applications as those known for their precursors before transformation and their nanostructures are accompanied by new properties allowing applications. This chapter briefly describes the synthesis process of the different NS TiO2, their chemical and surface modifications, and their application. The preparation of NS TiO2, including nanoparticles, nanorods, nanowires, nanosheets, nanofibers, and nanotubes is described. This chapter discusses the effects of precursor properties and synthesis conditions on the structure, crystallinity, surface specificity, and morphology of titanium dioxide nanoparticles. Recent advances in NS TiO2 in nano-biosensing, medical implants, drug delivery, and antibacterial fields, pharmaceutical applications, as well as their toxicity and biocompatibility, were presented