Revenue, trade and welfare effects of the COMESA FTA on the Democratic Republic of Congo

The present research attempts to assess the likely revenue, trade and welfare implications of the Common Market for Eastern and Southern Africa (COMESA) Free Trade Agreement (FTA) on the Democratic Republic of Congo (DRC). The study adopts a partial equilibrium model based on the World Integrated Trade Solution 2010 database and the Software for Market Analysis and Restrictions on Trade (WITS-SMART) as the methodological approach. The findings of the research reveal that the COMESA FTA will be beneficial to the DRC in terms of an increase in exports of US$371.57 million and consumer welfare gain amounting to US$28.49 million. Moreover, The WITS-SMART simulation results indicate that around US$322.10 million of trade will be created in the DRC as a result of the COMESA FTA. Notwithstanding the fact that trade creation will have a positive effect on welfare, as the Congolese consumers would benefit from lower prices, some local industries in the DRC may be threaten of closure due to the lack of competitiveness. In addition, the simultation results show that the country will experience a huge fiscal revenue loss amounting to US$107.01 million due to the implementation of zero per cent tarrif rate on imports duty from the COMESA trading partners. Finally, the simultation results indicate that an equivalent value of US$49.47 million of trade will be diverted from more efficient and low cost non-member states to high cost suppliers from the COMESA region. In light of these results, the research recommends that the DRC’s government needs to come up with a strategic plan in order to protect the national industry that would be negatively affected by the trade-creation effect. In order to mitigate the expected revenue loss, the implementation of the COMESA FTA in the DRC should be accompanied by fiscal reforms to improve the tax-collection system from sales or value-added taxes (VAT) and domestic excise. Regarding the trade-diversion effect, the inefficient producers from the COMESA region could be displaced through building new capacities in short, medium and long term based on comparative advantage in order to address supply constraints in these sectors affected by trade diversion

Contributors to Students’ Use of Counselling Services in Kenyan Universities

Transition to university life can be stressful for all students. In mitigation, most universities including those in Kenya offer social support to students in form of counselling, financial assistance, health and academic support. Despite this it has been documented that only a minority of university students who experience psychological distress seek professional counselling. This paper looks at contributors to students’ use of counselling services in Kenyan universities. These are classified into four: the social and behavioural issues, academic issues, psychological issues and economic issues. The study applied descriptive survey research design guided by Person Centred and Social Learning Theories. Data was collected using questionnaires, in-depth interview schedules and Focus Group Discussions. The study findings indicate that both male and female students are faced with many counselling issues such as academic, psychological, social, personal, economic, health, physical, vocational and spiritual .It can be concluded that the threat to masculine ideology encourages males to have more positive attitude towards seeking help for academic issues and that female students seek social psychological counselling to help them gain understanding of root causes of their problems. Key words: reasons, social and behavioural issues, academic issues, psychological issues, economic issue

Reasons Why University Students Do Not Seek Counselling Services in Kenya

Transition to university life can be stressful for all students. In mitigation, most universities in Kenya offer social support to students in form of counselling, financial assistance, health and academic support. Despite this it has been documented that only a minority of university students who experience psychological distress seek professional counselling (Khan and Williams, 2003, Raunic and Xenos, 2008) a situation that is the same in Kenya. According to university counsellors, university students undergo tremendous stress from personal issues, academic pressure, career emphasis and social problems. Counselling is meant to provide linear paths for students to address their problems. However, some students are reluctant to seek help from counsellors. This paper is a report on a study that sought to establish the reasons why university students in Kenya do not seek counselling services. Key words: Gender, Social Mistrust, Confidence, immediate solutions, Perception, Locatio

Assessing land use-land cover changes and their effects on the hydrological responses within the Nyangores River Catchment, Kenya

Philosophiae Doctor - PhDThis thesis aimed at contributing knowledge on how the widespread changes in land use/cover resulting from increasing human population and their associated activities, are influencing hydrological responses in a sub-humid catchment. The study therefore hypothesised that reduced forest cover over time in favour of agricultural activities is altering hydrological processes of the catchment which is affecting the flow characteristics in a sub-humid catchment. The sub-humid catchment selected to investigate these issues is the Nyangores River Catchment in Kenya

Prevalence of Counselling Services among University Students in Kenya

Transition to university life can be stressful for all students. In mitigation, most universities, both private and public, including those in Kenya offer social support to students in form of counselling, financial assistance, health and academic support. Despite this it has been documented that only a minority of university students who experience psychological distress seek professional counselling. This paper aimed at establishing the prevalence of counselling services among university students in Kenya. The study applied descriptive survey research design and guided by Person Centred and Social Learning Theories. Data was collected using questionnaires, in-depth interview schedules and Focus Group Discussions. Findings reveal that university students are faced with various life challenges such as academic, psychological, social, personal, economic, health, physical, vocational and spiritual. However, only 35% of students with issues in both private and public universities seek counselling services, either frequently or rarely, majority of which are females. The study recommends that university counsellors should initiate vigorous campaign to encourage male/female students to seek for counselling services. Key words Prevalence, gender, frequenc

Niemann-Pick C1 (NPC1)/NPC1-like1 Chimeras Define Sequences Critical for NPC1’s Function as a Filovirus Entry Receptor

We recently demonstrated that Niemann-Pick C1 (NPC1), a ubiquitous 13-pass cellular membrane protein involved in lysosomal cholesterol transport, is a critical entry receptor for filoviruses. Here we show that Niemann-Pick C1-like1 (NPC1L1), an NPC1 paralog and hepatitis C virus entry factor, lacks filovirus receptor activity. We exploited the structural similarity between NPC1 and NPC1L1 to construct and analyze a panel of chimeras in which NPC1L1 sequences were replaced with cognate sequences from NPC1. Only one chimera, NPC1L1 containing the second luminal domain (C) of NPC1 in place of its own, bound to the viral glycoprotein, GP. This engineered protein mediated authentic filovirus infection nearly as well as wild-type NPC1, and more efficiently than did a minimal NPC1 domain C-based receptor recently described by us. A reciprocal chimera, NPC1 containing NPC1L1’s domain C, was completely inactive. Remarkably, an intra-domain NPC1L1-NPC1 chimera bearing only a ~130-amino acid N–terminal region of NPC1 domain C could confer substantial viral receptor activity on NPC1L1. Taken together, these findings account for the failure of NPC1L1 to serve as a filovirus receptor, highlight the central role of the luminal domain C of NPC1 in filovirus entry, and reveal the direct involvement of N–terminal domain C sequences in NPC1’s function as a filovirus receptor

Mucosal immune profiles associated with diarrheal disease severity in shigella - and enteropathogenic escherichia coli-infected children enrolled in the global enteric multicenter study

Enteropathogenic Escherichia coli (EPEC) and Shigella are etiologic agents of diarrhea in children <5 years old living in resource-poor countries. Repeated bouts of infection lead to lifelong morbidity and even death. The goal of this study was to characterize local mucosal immune responses in Shigella- and EPEC-infected children <5 years of age with moderate to severe diarrhea (MSD) enrolled in the Global Enteric Multicenter Study (GEMS). We hypothesized that infection with each of these pathogens would induce distinct gut mucosal immune profiles indicative of disease etiology and severity. To test this hypothesis, innate and adaptive immune markers were measured in stools from children with diarrhea due to EPEC, Shigella, or other organisms and in children who had no diarrhea. Shigella-positive diarrhea evoked robust proinflammatory and TH1/TH2 cytokine responses compared to diarrhea caused by EPEC or other organisms, with the exception of interleukin 5 (IL-5), which was associated with EPEC infection. The presence of IL-1β, IL-4, IL-16, and tumor necrosis factor beta (TNF-β) was associated with the absence of dysentery. EPEC-positive diarrhea evoked high levels of IL-1β, vascular endothelial growth factor (VEGF), and IL-10. Granulocyte-macrophage colony-stimulating factor (GM-CSF) had opposing roles in disease severity, being associated with absence of diarrhea in EPEC-infected children and with dysenteric Shigella infection. High levels of antigen-specific antibodies were detected in the controls and children with Shigella without dysentery, which suggests a protective role against severe disease. In summary, this study identified distinct local immune responses associated with two clinically relevant diarrheagenic pathogens, Shigella and EPEC, in children and identified protective immune phenotypes that can inform the development of preventive measures

A Single Residue in Ebola Virus Receptor NPC1 Influences Cellular Host Range in Reptiles

ABSTRACT Filoviruses are the causative agents of an increasing number of disease outbreaks in human populations, including the current unprecedented Ebola virus disease (EVD) outbreak in western Africa. One obstacle to controlling these epidemics is our poor understanding of the host range of filoviruses and their natural reservoirs. Here, we investigated the role of the intracellular filovirus receptor, Niemann-Pick C1 (NPC1) as a molecular determinant of Ebola virus (EBOV) host range at the cellular level. Whereas human cells can be infected by EBOV, a cell line derived from a Russell’s viper (Daboia russellii) (VH-2) is resistant to infection in an NPC1-dependent manner. We found that VH-2 cells are resistant to EBOV infection because the Russell’s viper NPC1 ortholog bound poorly to the EBOV spike glycoprotein (GP). Analysis of panels of viper-human NPC1 chimeras and point mutants allowed us to identify a single amino acid residue in NPC1, at position 503, that bidirectionally influenced both its binding to EBOV GP and its viral receptor activity in cells. Significantly, this single residue change perturbed neither NPC1’s endosomal localization nor its housekeeping role in cellular cholesterol trafficking. Together with other recent work, these findings identify sequences in NPC1 that are important for viral receptor activity by virtue of their direct interaction with EBOV GP and suggest that they may influence filovirus host range in nature. Broader surveys of NPC1 orthologs from vertebrates may delineate additional sequence polymorphisms in this gene that control susceptibility to filovirus infection. IMPORTANCE: Identifying cellular factors that determine susceptibility to infection can help us understand how Ebola virus is transmitted. We asked if the EBOV receptor Niemann-Pick C1 (NPC1) could explain why reptiles are resistant to EBOV infection. We demonstrate that cells derived from the Russell’s viper are not susceptible to infection because EBOV cannot bind to viper NPC1. This resistance to infection can be mapped to a single amino acid residue in viper NPC1 that renders it unable to bind to EBOV GP. The newly solved structure of EBOV GP bound to NPC1 confirms our findings, revealing that this residue dips into the GP receptor-binding pocket and is therefore critical to the binding interface. Consequently, this otherwise well-conserved residue in vertebrate species influences the ability of reptilian NPC1 proteins to bind to EBOV GP, thereby affecting viral host range in reptilian cells

Ralstonia syzygii, the Blood Disease Bacterium and Some Asian R. solanacearum Strains Form a Single Genomic Species Despite Divergent Lifestyles

The Ralstonia solanacearum species complex includes R. solanacearum, R. syzygii, and the Blood Disease Bacterium (BDB). All colonize plant xylem vessels and cause wilt diseases, but with significant biological differences. R. solanacearum is a soilborne bacterium that infects the roots of a broad range of plants. R. syzygii causes Sumatra disease of clove trees and is actively transmitted by cercopoid insects. BDB is also pathogenic to a single host, banana, and is transmitted by pollinating insects. Sequencing and DNA-DNA hybridization studies indicated that despite their phenotypic differences, these three plant pathogens are actually very closely related, falling into the Phylotype IV subgroup of the R. solanacearum species complex. To better understand the relationships among these bacteria, we sequenced and annotated the genomes of R. syzygii strain R24 and BDB strain R229. These genomes were compared to strain PSI07, a closely related Phylotype IV tomato isolate of R. solanacearum, and to five additional R. solanacearum genomes. Whole-genome comparisons confirmed previous phylogenetic results: the three phylotype IV strains share more and larger syntenic regions with each other than with other R. solanacearum strains. Furthermore, the genetic distances between strains, assessed by an in-silico equivalent of DNA-DNA hybridization, unambiguously showed that phylotype IV strains of BDB, R. syzygii and R. solanacearum form one genomic species. Based on these comprehensive data we propose a revision of the taxonomy of the R. solanacearum species complex. The BDB and R. syzygii genomes encoded no obvious unique metabolic capacities and contained no evidence of horizontal gene transfer from bacteria occupying similar niches. Genes specific to R. syzygii and BDB were almost all of unknown function or extrachromosomal origin. Thus, the pathogenic life-styles of these organisms are more probably due to ecological adaptation and genomic convergence during vertical evolution than to the acquisition of DNA by horizontal transfer