Studies on antidyslipidemic effects of Morinda citrifolia (Noni) fruit, leaves and root extracts

<p>Abstract</p> <p>Background</p> <p>The objective of present study was to provide the pharmacological basis for the medicinal use of <it>Morinda citrifolia </it>Linn in dyslipidemia using the aqueous-ethanolic extracts of its fruits (Mc.Cr.F), leaves (Mc.Cr.L) and roots (Mc.Cr.R).</p> <p>Results</p> <p>Mc.Cr.F, Mc.Cr.L and Mc.Cr.R showed antidyslipidemic effects in both triton (WR-1339) and high fat diet-induced dyslipidemic rat models to variable extents. All three extracts caused reduction in total cholesterol and triglyceride levels in triton-induced dyslipidemia. In high fat diet-induced dyslipidemia all these extracts caused significant reduction in total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C), atherogenic index and TC/HDL ratio. Mc.Cr.R extract also caused increase in high density lipoprotein-cholesterol (HDL-C). The Mc.Cr.L and Mc.Cr.R reduced gain in body weight with a reduction in daily diet consumption but Mc.Cr.F had no effect on body weight and daily diet consumption.</p> <p>Conclusions</p> <p>These data indicate that the antidyslipidemic effect of the plant extracts was meditated through the inhibition of biosynthesis, absorption and secretion of lipids. This may be possibly due partly to the presence of antioxidant constituents in this plant. Therefore, this study rationalizes the medicinal use of <it>Morinda citrifolia </it>in dyslipidemia.</p

6 Minute Walk Test (6MWT) for Healthy Adult Volunteers

Objectives: To determine the 6-minute walk test (6MWT) for healthy volunteers in our population and find out the factors affecting 6MWT. Furthermore, comparing our results with published National and International data. Study Design: Cross-sectional study. Place and Duration of Study: This study was carried in the physiology department of Rawalpindi Medical College, from 1st July 2019 to 31st December 2019. Materials and Methods: A total of 129 adult healthy volunteers between 18 and 60 years of age were included in this study after the application of inclusion and exclusion criteria. A 6-minute walk test (6MWT) was carried out in a standardized procedure. The height, weight, and BMI were recorded on predesigned Performa. Distance covered in 6-minute, heart rate, systolic BP, Diastolic BP, SPO2, Borg Scale Dyspnea score, Borg Scale Fatigue score were recorded before and after the 6-minute walk test. Correlation and its significance were calculated among anthropometric and physiological variables. Results: Seventy (54%) men and Fifty-nine (46%) women participated in the study. The mean distance covered for all subjects was 430.412±63.0150. The distance covered by the female subject was 388.720±30.104 and 439.533±68.355 by male subjects. The mean age of the subjects was 31.64 ±12.36, while the mean BMI was 22.06 ±4.15. The mean heart rate value before the test is 77.72±1.48 and after the test is 92.84±1.12. The mean systolic BP value before the walk was 122.65±11.75 and after the walk, it was 106.5±9.83. Mean diastolic BP before the walk was 80.55±12.61 and after the walk, it was 76.5±9.83. SPO2 mean value before the walk was 96.23±1.4 and after the walk is 98.2±1.17. Borg scale dyspnea score mean value before the test is 0 ± 0 and after the test it was 0.67±0.76 and Borg scale fatigue score mean value before the walk is 1.32±0.73 and after the walk is 1.62±0.92. A positive correlation was noted with height, while a negative correlation was noted with age and BMI. Gender also showed a significant relationship with the 6MWD. Conclusion: The distance covered in 6-minute is shorter than the studies carried out in different parts of the world. Age, gender, height, and BMI showed a significant relationship with 6MWD. Those reference values for the 6MWD should not be used in our population, as it overestimates the distance and may interfere with the significance of the test

Genetic characterization of small ruminant morbillivirus from recently emerging wave of outbreaks in Pakistan

Peste des petits ruminants (PPR) is an endemic and highly infectious disease of several tropical countries, including Pakistan. Despite exhaustive vaccination, outbreaks are on the rise annually across different parts of the country. Clinical outcomes are largely employed to diagnose disease, while comprehensive genomic features of prevailing field strains of SRMV largely remain elusive. Here, we present comparative sequence-based phylogenomics of field strains from three districts representing different agro-livestock production systems during an emerging wave of outbreaks in 2015, together with the first complete genome sequencing of a strain from Pakistan. The analysis revealed clustering of study strain (SRMV/Lahore/UVAS/Pak/2015) to lineage IV, close to isolates characterized previously from India and China. Investigations of inter- and intralineage genetic distances showed a higher genetic distance between SRMV/Lahore/UVAS/Pak/2015 to lineage III viruses than lineages I and II. The characterized Pakistani strain also showed a high percentage of genetic distance from isolates originated from Nigerian and other African small ruminants. Based on these observations, an integrated cross-protection investigation is warranted in the future, not only to define the protective efficacy of currently applied vaccines, but also to continuously elucidate the genomic and evolutionary nature of circulating viruses in the country to achieve disease eradication by 2030

ASSESSMENT AND IMPORTANCE OF LIPID PROFILE IN PRE-ECLAMPSIA WOMEN

Objective: The aim of this study is to know the assessment and importance of lipid profile in preeclampsia women. Study Design: A cross-sectional analytical study. Place and Duration: In the Physiology Department of Nishtar Medical University, Multan in Collaboration with Gynecology and Obstetrics Department of Nishtar Hospital Multan for One year Duration from September 2017 to September 2018. Methods: The study consisted of 90 subjects, each of which consisted of 30 people. 30 were apparently healthy subjects, 30 mild preeclamptic and 30 severe preeclamptic. Clinical details were collected. Fasting blood samples were obtained by aseptic methods and serum lipid profile was analyzed. Results: Of the 90 subjects studied, 30 had normal triglycerides (176.76 mg / d1). However, 30 subjects with severe preeclampsia show serum triglycerides 242.mg / dl according to normal. The difference in both groups was statistically more significant than normal. Conclusion: It was concluded that high circulating levels increased with preeclampsia in the pathogenesis of preeclampsia associated with preeclampsia, which may be a risk factor during pregnancy. Key words: Lipids, Preeclampsia

A Nonlinear Structure of a Chemical Reaction Model and Numerical Modeling with the New Aspect of Existence and Uniqueness

In this article, a nonlinear autocatalytic chemical reaction glycolysis model with the appearance of advection and diffusion is proposed. The occurrence and unicity of the solutions in Banach spaces are investigated. The solutions to these types of models are obtained by the optimization of the closed and convex subsets of the function space. Explicit estimates of the solutions for the admissible auxiliary data are formulated. An elegant numerical scheme is designed for an autocatalytic chemical reaction model, that is, the glycolysis model. The fundamental traits of the prescribed numerical method, for instance, the positivity, consistency, stability, etc., are also verified. The authenticity of the proposed scheme is ensured by comparing it with two extensively used numerical techniques. A numerical example is presented to observe the graphical behavior of the continuous system by constructing the numerical algorithm. The comparison depicts that the projected numerical design is more productive as compared to the other two schemes, as it holds all the important properties of the continuous model.Basque Government, Grant IT1555-22 and Grant KK-2022/00090 MCIN/AEI 269.10.13039/ 501100011033, Grant PID2021-1235430B-C21/C22

Studies on two polyherbal formulations (ZPTO and ZTO) for comparison of their antidyslipidemic, antihypertensive and endothelial modulating activities

Background Cardiovascular disorders (CVDs) are the leading cause of disease burden worldwide. Apart from available synthetic drugs used in CVDs, there are many herbal formulations including POL-10 (containing 10 herbs), which have been shown to be effective in animal studies but POL-10 was found to cause tachycardia in rodents as its side effect. This study was designed to modify the composition of POL-10 for better efficacy and/or safety profile in CVDs. Methods To assess the antidyslipidemic, antihypertensive and endothelial modulatory properties of two herbal formulations, (ZPTO and ZTO) containing Z: Zingiber officinalis, P: Piper nigrum, T: Terminalia belerica and O: Orchis mascula, different animal models including, tyloxapol and high fat diet-induced dyslipidemia and spontaneously hypertensive rats (SHR) were used. Effect on endothelial function was studied using isolated tissue bath set up coupled with PowerLab data acquisition system. The antioxidant activity was carried out using DPPH radical-scavenging assay. Results Based on preliminary screening of the ingredients of POL-10 in tyloxapol-induced hyperlipidemic rats, ZPTO and ZTO containing four active ingredients namely; Z, P, T and O were identified for further studies and comparison. In tyloxapol-induced hyperlipidemic rats, both ZPTO and ZTO caused significant reduction in serum triglyceride (TG) and total cholesterol (TC). In high fat diet-fed rats, ZPTO decreased TC, low-density lipoproteins cholesterol (LDL-C) and atherogenic index (AI). ZTO also showed similar effects to those of ZPTO with additional merits being more effective in reducing AI, body weight and more importantly raising high-density lipoproteins. In SHR, both formulations markedly reduced systolic blood pressure, AI and TG levels, ZTO being more potent in reversing endothelial dysfunction while was devoid of cardiac stimulatory effect. In addition, ZTO also reduced LDL-C and improved glucose levels in SHR. In DPPH radical-scavenging activity test, ZTO was also more potent than ZPTO. Conclusion The modified formulation, ZTO was not only found more effective in correcting cardiovascular abnormalities than ZPTO or POL-10 but also it was free from tachycardiac side-effect, which might be observed because of the presence of Piper nigrum in ZPTO

Suppression of Phase Separation in LiFePO4 Nanoparticles During Battery Discharge

Using a novel electrochemical phase-field model, we question the common belief that LixFePO4 nanoparticles separate into Li-rich and Li-poor phases during battery discharge. For small currents, spinodal decomposition or nucleation leads to moving phase boundaries. Above a critical current density (in the Tafel regime), the spinodal disappears, and particles fill homogeneously, which may explain the superior rate capability and long cycle life of nano-LiFePO4 cathodes.Comment: 27 pages, 8 figure