Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank.

When it comes to biobanking and working with different types of laboratory specimens, it is important to understand potential biohazards to ensure safety of the operator and laboratory personnel. Biological safety levels (BSL) are a series of designations used to inform laboratory personnel about the level of biohazardous risks in a laboratory setting. There are a total of four levels ranked in order of increasing risk as stipulated by the Center of Disease Control and Prevention (CDC) (Biosafety in microbiological and biomedical laboratories, 5th edn. HHS publication no. (CDC) 21-1112. https://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf . Accessed 2 Jan 2016, 2009). We will address the main distinctions between these levels including briefly introducing hazards characteristics that classify biohazardous agents, as well as define the essentials in meeting safety requirements

Powers of sets in free groups

We prove that |A^n| > c_n |A|^{[\frac{n+1}{2}]} for any finite subset A of a free group if A contains at least two noncommuting elements, where c_n>0 are constants not depending on A. Simple examples show that the order of these estimates are the best possible for each n>0.Comment: 3 page

Thermal testing by internal IR heating of the FEP module

A spacecraft module, to be integrated with the FLTSATCOM spacecraft, was tested in a simulated orbit environment separate from the host spacecraft. Thermal vacuum testing of the module was accomplished using internal IR heating rather than conventional external heat sources. For this configuration, the technique produced boundary conditions expected for flight to enable verification of system performance and thermal design details

ECG Wave-Maven: An Internet-based Electrocardiography Self-Assessment Program for Students and Clinicians

Purpose: To create a multimedia internet-based ECG teaching tool, with the ability to rapidly incorporate new clinical cases. Method: We created ECG Wave-Maven (http://ecg.bidmc.harvard.edu), a novel teaching tool with a direct link to an institution-wide clinical repository. We analyzed usage data from the web between December, 2000 and May 2002. Results: In 17 months, there have been 4105 distinct uses of the program. A majority of users are physicians or medical students (2605, 63%), and almost half report use as an educational tool. Conclusions: The internet offers an opportunity to provide easily-expandable, open access resources for ECG pedagogy which may be used to complement traditional methods of instructio

Continuity of the roots of a polynomial

Let $K$ be an algebraically closed field with an absolute value. This note gives an elementary proof of the classical result that the roots of a polynomial with coefficients in $K$ are continuous functions of the coefficients of the polynomial.Comment: 9 pages; minor improvement

IMMUNOPATHOGENESIS OF ACUTE CENTRAL NERVOUS SYSTEM DISEASE PRODUCED BY LYMPHOCYTIC CHORIOMENINGITIS VIRUS : II. ADOPTIVE IMMUNIZATION OF VIRUS CARRIERS

Lymphocytic choriomeningitis (LCM) virus carriers were established by intracerebral inoculation of adult BALB/c mice followed by a single dose of cyclophosphamide (CY) (150 mg/kg) 3 days after infection, and by intracerebral injection within 24 hr of birth. These carriers were then adoptively immunized with spleen cells or serum from immune or normal BALB/c donors. Transfer of immune spleen cells into drug-induced carriers consistently resulted in acutely fatal choriomeningitis, histologically strikingly similar to classical LCM. Normal spleen cells or immune serum failed to produce either central nervous system (CNS) pathology or illness with any regularity. In addition, focal necrosis of the cerebellum was seen after adoptive immunization of drug-induced carriers but only when mice received cells at least 3 wk after inoculation, which is probably explained by the gradual spread of infection from membranes to the neural parenchyma during the first month after establishment of the carrier state in adult mice. Immune spleen cells, when transferred to neonatal carriers, led to a decrease in virus titers in blood and brains and to development of antibody without acute CNS disease. It appears that the production of fatal choriomeningitis after LCM infection is determined in part by the distribution of viral antigen, and this is markedly different in neonatal and drug-induced carriers at the time of cell transfer. Another factor of potential importance is the much higher level of circulating viral antigen in the plasma of neonatal than in that of drug-induced LCM carriers. Classical LCM disease can only be transferred by immune lymphoid cells and not by antiserum. Furthermore, little or no complement-fixing (CF) antibody was found in the plasma of mice dying of acute choroiditis. These observations strongly suggest that acute choroiditis is dependent upon the cell-mediated immune response

SIC-POVMs and the Extended Clifford Group

We describe the structure of the extended Clifford Group (defined to be the group consisting of all operators, unitary and anti-unitary, which normalize the generalized Pauli group (or Weyl-Heisenberg group as it is often called)). We also obtain a number of results concerning the structure of the Clifford Group proper (i.e. the group consisting just of the unitary operators which normalize the generalized Pauli group). We then investigate the action of the extended Clifford group operators on symmetric informationally complete POVMs (or SIC-POVMs) covariant relative to the action of the generalized Pauli group. We show that each of the fiducial vectors which has been constructed so far (including all the vectors constructed numerically by Renes et al) is an eigenvector of one of a special class of order 3 Clifford unitaries. This suggests a strengthening of a conjuecture of Zauner's. We give a complete characterization of the orbits and stability groups in dimensions 2-7. Finally, we show that the problem of constructing fiducial vectors may be expected to simplify in the infinite sequence of dimensions 7, 13, 19, 21, 31,... . We illustrate this point by constructing exact expressions for fiducial vectors in dimensions 7 and 19.Comment: 27 pages. Version 2 contains some additional discussion of Zauner's original conjecture, and an alternative, possibly stronger version of the conjecture in version 1 of this paper; also a few other minor improvement

IMMUNOPATHOGENESIS OF ACUTE CENTRAL NERVOUS SYSTEM DISEASE PRODUCED BY LYMPHOCYTIC CHORIOMENINGITIS VIRUS : I. CYCLOPHOSPHAMIDE-MEDIATED INDUCTION OF THE VIRUS-CARRIER STATE IN ADULT MICE

A single dose of 150 mg/g of cyclophosphamide (CY), given 3 days after intracerebral (i.c.) inoculation of lymphocytic choriomeningitis (LCM) virus, protected over 90% of adult BALB/c mice against acutely fatal choriomeningitis. Surviving mice became persistently infected carriers, with high virus titers in blood and brain. Immunofluorescent examination of the brain showed that in CY-induced carriers infection was initially confined to the choroid plexus, ependyma, and leptomeninges, but over the next 30 days gradually spread to the neural parenchyma, most notably to the molecular layer of the cerebellum. By contrast, LCM virus-carrier mice produced by neonatal virus injection and examined as adults, showed a much less marked infection of choroid plexus and much more widespread infection of parenchyma, with a different distribution among brain nuclei, including heavy infection of the Purkinje cells of the cerebellum