Aperfeiçoamento de Abordagens para o Recrutamento de Pessoal na Indústria Hoteleira

The purpose of the article is to develop a recommendation system for hospitality enterprises using non-traditional forms of recruitment. The dynamism of changes in the environment is found to necessitate the use of innovative approaches based on the real needs and opportunities of modern hospitality industry enterprises. It is confirmed that the effectiveness of personnel recruitment depends on the mastery of up-to-date methods. It is established that implementing the totality of the proposed modern human resource management technologies will contribute to increased personnel productivity and efficiency of hotel enterprises. The introduction and implementation of innovative technologies of personnel management are found to bring changes to other resource areas – in the nature of the hotel product, in the way relationships with the key customers are constructed, and in the economy of the hotel enterprise as a whole in a digitalized environment.El objetivo del artículo es desarrollar un sistema de recomendación para empresas de hostelería que utilicen formas de contratación no tradicionales. Se considera que el dinamismo de los cambios en el entorno requiere el uso de enfoques innovadores basados en las necesidades y oportunidades reales de las empresas modernas de la industria hotelera. Se confirma que la eficacia de la contratación de personal depende del dominio de los métodos actualizados. Se establece que la implementación de la totalidad de las tecnologías modernas de gestión de recursos humanos propuestas contribuirá a aumentar la productividad del personal y la eficiencia de las empresas hoteleras. Se encuentra que la introducción e implementación de tecnologías innovadoras de gestión de personal trae cambios a otras áreas de recursos: en la naturaleza del producto hotelero, en la forma en que se construyen las relaciones con los clientes clave y en la economía de la empresa hotelera en su conjunto. en un entorno digitalizado.O objetivo do artigo é desenvolver um sistema de recomendação para empresas de hospitalidade usando formas não tradicionais de recrutamento. O dinamismo das mudanças no ambiente exige o uso de abordagens inovadoras baseadas nas reais necessidades e oportunidades das empresas modernas da indústria hoteleira. Confirma-se que a eficácia do recrutamento de pessoal depende do domínio de métodos atualizados. Fica estabelecido que a implementação da totalidade das modernas tecnologias de gestão de recursos humanos propostas contribuirá para o aumento da produtividade do pessoal e da eficiência das empresas hoteleiras. A introdução e implementação de tecnologias inovadoras de gestão de pessoal trazem mudanças para outras áreas de recursos – na natureza do produto hoteleiro, na forma como os relacionamentos com os principais clientes são construídos e na economia da empresa hoteleira como um todo em um ambiente digitalizado

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p53 Protects renal inner medullary cells from hypertonic stress by restricting DNA replication

Dmitrieva, Natalia, Luis Michea, and Maurice Burg. p53 Protects renal inner medullary cells from hypertonic stress by restricting DNA replication. Am J Physiol Renal Physiol 281: F522-F530, 2001.-We previously found that p53 upregulation by hypertonicity protected renal inner medullary collecting duct (mIMCD3) cells from apoptosis. The purpose of the present study was to investigate the mechanism by which p53 protects the cells. We now find that hypertonicity (NaCl added to a total of 500 mosmol) inhibits DNA replication and delays G 1-S transition as concluded from analysis of cell cycle distributions and bromodeoxyuridine (BrDU) incorporation rates. Lowering of p53 with p53 antisense oligonucleotide attenuated such effects of hypertonicity, resulting in an increased number of apoptotic cells in S phase and cells with Ͼ4 N DNA. Results with synchronized cells are similar, showing that cells in the early S phase are more sensitive to hypertonicity. Immunocytochemistry revealed that p53 becomes phosphorylated on Ser 15 and translocates to the nucleus in S both in isotonic and hypertonic conditions. Caffeine (2 mM) greatly reduces the p53 level and Ser 15 phosphorylation, followed by a remarkable increase of DNA replication rate, by failure of hypertonicity to inhibit it, and by reduction of cell number during hypertonicity. Finally, inhibition of DNA replication by the DNA polymerase inhibitor aphidicolin significantly improves cell survival, confirming that keeping cells in G1 and decreasing the rate of DNA replication is protective and that these actions of p53 most likely are what normally help protect cells against hypertonicity. cell cycle arrest; apoptosis; sodium chloride CELLS OF THE renal inner medulla are normally exposed to variable and often extreme hypertonicity as the result of the renal mechanism for concentrating the urine. This raises questions about the mechanisms that they employ to survive and function under such adverse conditions. p53 Is a tumor suppressor whose loss of function, observed in many types of cancer, contributes to genomic instability and malignancy In previous studies, we found that hypertonicity increased the amount of total and phosphorylated Ser 15 p53-and p53-dependent transcription in renal inner medullary collecting duct cells (mIMCD3; see Ref. 11). Under these conditions, reducing p53 with p53 antisense oligonucleotide (p53-AS) increased apoptosis, suggesting that activation of p53 is protective (11). Hypertonicity also arrests growth of mIMCD3 cells The purpose of present study was to analyze the mechanism by which p53 protects against hypertonicity. We found that phosphorylation of p53 on Ser 15 , previously noted to be protective during hypertonicity, occurs in the S phase of the cell cycle. Furthermore, reducing p53 expression with p53-AS or caffeine reversed both the G 1 -S arrest and reduction of DNA replication that are caused by hypertonicity. Under those conditions, apoptosis increased mainly in the cells in which DNA content had increased. We conclude that p53 protects cells against hypertonic stress by restricting DNA replication. EXPERIMENTAL PROCEDURES Cell culture. Subconfluent cultures of mIMCD3 cells (generously provided by S. Gullans; see Ref. 31) were used in passages 13-17. The medium contained 45% DME low glucose, 45% Coon's Improved Medium mF-12 (Irvine Scientific), and 10% FBS (Life Technologies). Osmolality of control ("isotonic") medium was 320 mosmol/kg. Hypertonic media, prepared by adding NaCl, were substituted for the control medium, as indicated. Cells were incubated at 37°C and gassed with 5% CO 2-95% air during growth and during all experiments. Antisense oligonucleotide experiments. For all experiments with p53-AS (Biognostik), cells were grown on eight-chamber plastic slides (Nalge Nunc International) and preincubated for 16 h with 2 M of p53-AS (sequence: CGT CAT GTG CTG TGA C) or control (CG-matched randomized-sequence phosphorothioate oligonucleotide: GAC TAC GAC CTA CGT G)

FEATURES OF THE DEVELOPMENT OF A LYOPHILIZED INJECTABLE DOSAGE FORM OF THE ORIGINAL ANTICANCER DRUG LCS-1208

Objective: Development of a lyophilized injectable dosage form LCS-1208, an original antitumor drug based on an indolocarbazole derivative. Methods: The prepared solution of the injectable dosage form LCS-1208 is transferred to sterilizing filtration, which is carried out under vacuum on a «Stericup» filter unit with a filter pore size of 0.22 μm. The sterile solution of the injectable dosage form LCS-1208 is poured into sterile vials using a dispenser and lyophilized in a freeze-drying chamber. At the end of drying, the preparation is corked in the chamber of a sublimation unit using a hydraulic device and transferred to crimping with aluminum caps using a seaming machine. Quantitative determination of the drug content was determined by spectrophotometry using a standard sample at λ = 320±2 nm. The pH was determined by potentiometry. Results: A freeze-drying regimen for the injectable dosage form LCS-1208 has been developed. The required solution freezing temperature was established taking into account the presence of 2 eutectic zones: a solution of LCS-1208 in DMSO (-35 ÷-32) °С, an aqueous solution of Kollidon 17PF (-10 ÷-8) °С. As a result of a series of experiments, the optimal lyophilization regime was chosen that does not require preliminary freezing in a low-temperature chamber, with freezing on the shelves of freeze-drying at a temperature of-47 °C without their preliminary cooling. The most acceptable vial filling volume was determined, amounting to 3 ml, and the rate of temperature rise during secondary drying of the preparation was justified. When using the developed regime of lyophilization of the LCS-1208 solution, it was shown that it can be sublimated while preserving the initial qualitative and quantitative characteristics. Conclusion: In this article, using the example of creating a lyophilized injectable dosage form LCS-1208 (the original antitumor drug from the indolocarbazole group), the main problems that arose during the lyophilization of the selected composition of the model solution, as well as ways to improve the process

Ontogenetic Development of Neural and Muscular Rhythmic Activity and Its Regulation in Mammals during Perinatal Period

This review covers our recent advantages in studying the ontogenetic aspects of physiological mechanisms underlying regulation of rhythmic behavior. We have revealed that excitation patterns that emerged at early stages of phylogenetic development of life forms contribute greatly to the rhythmic activity of living vertebrates and invertebrates. These patterns govern spontaneous excitation, which is easily observed during the early stage of ontogenesis. The intensity and patterns of rhythmic activity are determined by nature and kinetics of certain metabolic reactions. During perinatal and sometimes postnatal periods (as in prematurely born animals), endogenic rhythmicity of developing physiological structures is strongly pronounced due to relatively stable living conditions. This rhythmic behavior is coordinated within an entire organism. Its integration in multiple systems is driven by amplitude and frequency modulation yielding rhythms of various frequency ranges. Indeed, it is the complex and conjoint functioning of physiological systems that maintains homeostasis in developing organisms. We present the results of our authentic research concerning the evolution and ontogeny of regulatory mechanisms of motor, cardiovascular, and respiratory systems. The aspects of intact and disrupted development are considered, involving the changes in dopaminergic, norepinephrinergic, and cholinergic system activation

VALIDATION OF THE QUALITATIVE DETERMINATION OF HPLC METODS FOR SUCROSE AND PEG-2000-DSPE IN A LIPOSOMAL FORM OF THE PHOTOSENSITIZER LIPOPHTHALOCYAN

Objective: This study was undertaken with the aim of the validate the simple isocratic metods high-performance liquid chromatographic (HPLC) for the estimation of PEG-2000-DSPE and sucrose in liposomal medicinal formulations of the phthalocyanine photosensitizer Lipophthalocyan. Methods: HPLC quantification was carried out by using of YMC-Pack Polyamine II column. The mobile phase (for sucrose: acetonitrile: water: ethyl acetate in the ratio of 450: 200: 20; for PEG-2000-DSPE: water in the ratio 10: 90) was pumped at a flow rate of 1 ml/min. Following the guidelines of the International Conference on Harmonization (ICH), the methods was validated for various analytical parameters like specificity, linearity, detection limit, quantitative limit, correctness, and accuracy. Results: The obtained results of the analysis were validated statistically. The correlation coefficient for the linearity was 0.999292, for sucrose, and 0.997650 for PEG-2000-DSPE. The methods can be assessed as correct, as the results obtained are close to the true value and the confidence interval for both methods include 100%. The coefficients of variation in both methods in determining the accuracy were less than 3%. Conclusion: The proposed HPLC methods were validated according to the ICH guidelines and results and statistical parameters demonstrated that the developed methods are sensitive, precise, reliable and simple for the estimation of PEG-2000-DSPE and sucrose in Lipophthalocyan