Oral contrast-enhanced CT cholangiography--an initial experience

Objective: To describe our experience of CT (Computed tomographic) Cholangiography examination for detection of choledocholithiasis at the Aga Khan University Hospital (AKUH) Karachi Pakistan.Methods: Seven patients underwent helical CT cholangiography for suspected choledocholithiasis. Iopanoic acid (6 grams) was administered orally 8-12 hours before acquisition of a helical CT cholangiogram. Three-dimensional reconstructions and curved multiplanar reformations were generated from a set of axial source images.Results: Our patients had no adverse reactions to iopanoic acid. The degree of biliary opacification was sufficient to perform three-dimensional and curved planar reformations in 5 patients. In two patients, the biliary tree was not opacified. Both of these studies were considered failures. Findings on CT cholangiography in the remaining 5 patients were the following: cholelithiasis with normal bile duct (n=2), choledocholithiasis (n=1), stone in gallbladder remnant with long cystic duct (n=1) and infiltrating adenocarcinoma of the gallbladder (n=1).CONCLUSION: Spiral CT cholangiography is a safe, non-invasive, and cost effective alternative test and, in a carefully selected patient population can play a role in the diagnostic work-up of patients with suspected choledocholithiasis

Robust human face detection in complex color images

We propose in this paper a model based technique for the detection of human faces from rich still color images. Traditionally, color images are represented in the RGB color space. RGB space, however, is not only a 3-dimensional space but also includes brightness or luminance which is not a reliable criterion for skin separation. To avoid the effect of luminance, we propose to work in the chromatic or pure color space. Using such space, a Gaussian model for the skin color pixels is developed and a skin likelihood image is obtained. Such image is then transformed into a binary image using adaptive thresholding. Finally, bright regions satisfying certain "facial" properties are obtained followed by a template matching stage. The method presented here is shown to provide robust detection under different environments and found to achieve very satisfactory results when compared to traditional "mug shot" based approaches

Robust human face detection in complex color images

We propose in this paper a model based technique for the detection of human faces from rich still color images. Traditionally, color images are represented in the RGB color space. RGB space, however, is not only a 3-dimensional space but also includes brightness or luminance which is not a reliable criterion for skin separation. To avoid the effect of luminance, we propose to work in the chromatic or pure color space. Using such space, a Gaussian model for the skin color pixels is developed and a skin likelihood image is obtained. Such image is then transformed into a binary image using adaptive thresholding. Finally, bright regions satisfying certain "facial" properties are obtained followed by a template matching stage. The method presented here is shown to provide robust detection under different environments and found to achieve very satisfactory results when compared to traditional "mug shot" based approaches

Expression of M. tuberculosis-induced suppressor of cytokine signaling (SOCS) 1, SOCS3, FoxP3 and secretion of IL-6 associates with differing clinical severity of tuberculosis

Background Appropriate immune activation of T cells and macrophages is central for the control of Mycobacterium tuberculosis infections. IFN-γ stimulated responses are lowered in tuberculosis (TB), while expression of Suppressor of Cytokine Signaling (SOCS) molecules – 1 and 3 and CD4+CD25+FoxP3+T regulatory cells is increased. Here we investigated the association of these molecules in regard to clinical severity of TB. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from patients with pulmonary TB (PTB, n = 33), extra-pulmonary TB (ETB, n = 33) and healthy endemic controls (EC, n = 15). Cases were classified as moderately advanced or far advanced PTB, and less severe or severe disseminated ETB. M. tuberculosis -stimulated IFN-γ, SOCS1, SOCS3 and FoxP3 gene expression and secretion of Th1 and Th2 cytokines was measured. Statistical analysis was performed using Mann–Whitney U, Wilcoxon Rank and Kruskal Wallis non-parametric tests. Results In un-stimulated PBMCs, IL-6 (p = 0.018) and IL-10 (p = 0.013) secretion levels were increased in PTB while IL-10 was also increased in ETB (p = 0.003), all in comparison with EC. M. tuberculosis-stimulated IL-6 (p = 0.003) was lowered in ETB as compared with EC. SOCS1 mRNA expression in M. tuberculosis stimulated PBMCs levels in moderately advanced PTB (p = 0.022), far advanced (p = 0.014) PTB, and severe ETB (p = 0.009) were raised as compared with EC. On the other hand, SOCS1 mRNA titers were reduced in less severe ETB, in comparison with severe ETB (p = 0.027) and far advanced PTB (p = 0.016). SOCS3 mRNA accumulation was reduced in far advanced PTB (p = 0.007) and FoxP3 mRNA expression was increased in less severe ETB as compared with EC (p = 0.017). Conclusions The lowered SOCS1 mRNA levels in patients with less severe extra-pulmonary TB as compared to those with more severe ETB and PTB may lead to elevated IFN-γ pathway gene expression in the latter group. As localized ETB has shown to be associated with more effective Th1 immunity and adaptive responses, this suggests a role for SOCS1 in determining disease outcome in extra-pulmonary TB

Investigation into high-speed impact response of composite sandwich structures

© The Author(s) 2025. This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/)Sandwich structures composed of top and bottom face sheets and an inner core are commonly used for energy-absorbing applications, mainly because of their superior stiffness-to-weight ratio and crashworthiness. Despite extensive studies on the ballistic behavior of monolithic and composite materials, limited research has focused on hybrid sandwich structures combining lightweight and ductile materials like thermoplastic polyurethane (TPU) with high-strength aluminum. This study aimed to numerically establish the ballistic limit velocities and the penetrating and perforation resistances of composite sandwich structures to address this gap. The sandwich panels were manufactured from thermoplastic polyurethane (TPU) and aluminum (Al) 2024-T351 as core and face sheets/skins, respectively. The panels were subjected to an impact to investigate the effects of various thicknesses of their face skins and core on high-speed impact resistance. From the results obtained, it was evident that the numerical models simulated experiments with high accuracy. The impact and damage resistances of the composite sandwich structures increased with the thicknesses of their core and face sheets. The resistance of the structure increased by 19% by increasing the thickness of face sheets from 1.2 to 2.0 mm. Similarly, the resistance of the composites can be increased by 44% by increasing the core thickness from 20 to 50 mm. Therefore, it can be established that the impact resistance of the composite sandwich structures depended on the thicknesses of their core and skins. The investigated performances of the different composite sandwich structures should guide their choice for various industrial applications.Peer reviewe

An investigation of supervector regression for forensic voice comparison on small data

International audienceThe present paper deals with an observer design for a nonlinear lateral vehicle model. The nonlinear model is represented by an exact Takagi-Sugeno (TS) model via the sector nonlinearity transformation. A proportional multiple integral observer (PMIO) based on the TS model is designed to estimate simultaneously the state vector and the unknown input (road curvature). The convergence conditions of the estimation error are expressed under LMI formulation using the Lyapunov theory which guaranties bounded error. Simulations are carried out and experimental results are provided to illustrate the proposed observer

CHOLINE-UTILIZING MICROBLAL STRAINS FOR BIOLOGICALLY CONTROLLING FUSARIUM HEAD BLIGHT

Three choline utilizing strains of microorganisms isolated from the anthers of wheat, Aureobasidium pullulans strainAS 55.2, Arthrobacter species strain OH 221.3, and Pseudomonas species strain AS 64.4, are superior antagonists of F. graminearum. These microorganisms are effective for suppression and control of FHB in cereals, particularly in wheat and barley

Bacterial Shoot Apical Meristem Inoculation Assay

© Springer Science+Business Media, LLC, part of Springer Nature 2020. By virtue of their sessile nature, plants may not show the fight-and-flight response, but they are not devoid of protecting themselves from disease-causing agents, attack by herbivores, and damages that are caused by other environmental factors. Plants differentially protect their life-sustaining organs such as plant apexes from the attack by microbial pathogens. There are well-established methods to inoculate/infect various plant parts such as leaves, roots, and stems with various different pathogens. The plant shoot apical meristems (SAM) are a high-value plant target that provides niche to stem cell populations. These stem cells are instrumental in maintaining future plant progenies by giving birth to cells that culminate in flowers, leaves, and stems. There are hardly few protocols available that allow us to study immune dynamics of the plant stem cells as they are hindered by various layers of the SAM cell populations. Here, we describe a step-by-step method on how to inoculate the Arabidopsis SAM with model plant pathogen Pseudomonas syringae pv. tomato DC3000

A descriptive analysis of research culture in Pakistan

This study addresses evolution of research culture in Pakistan. The study is descriptive in nature. The primary analysis is related to 180 Pakistani universities and 13259 PhDs produced from these universities since 1947 till 2016 along with their different contextual ratios such as geography, gender, public, and private sectors, universities and number of produced PhDs, supervisor and number of produced PhDs ratios, era wise growth of number of universities and produced PhDs. The study accounts for benchmark changes in leadership and resources as impacting factors for change in number of universities and produced number of Doctoral Researches (PhDs). The primary source for data is HEC while respective websites of the universities (if available) are also explored for the missing data. The study documents relatively recent emergence of certain assets in terms of a research culture and anticipates an opportunity to build upon these assets in future. Public universities with more experience are taking lead in almost all domains such as increase in number of universities and PhDs produced, their geographic and gender diversity, and supervisor to PhDs ratio etc. and private and relevantly new universities lag behind. If these could also start contributing soon Pakistan could muster the fruit of a rich research culture