A test for multivariate skew-normality based on its canonical form

Abstract A test to assess if a sample comes from a multivariate skew-normal distribution is proposed. The test statistic is obtained from the canonical form of the multivariate skew-normal distribution and its null distribution is derived. The power of the proposed test is evaluated through Monte Carlo simulations for different conveniently chosen alternatives. Finally, three numerical examples are presented for the purpose of illustration

Prevalence and Susceptibility of extended spectrum beta-lactamases in urinary isolates of Escherichia coli in a Tertiary Care Hospital, Chennai- South India

Extended spectrum beta – lactamases (ESBLs) are on the rise in hospitalsettings across the globe. The presence of ESBLs significantly affects the outcome of an infection and poses a challenge to the management of infection worldwide. Therefore, the aim of the present study is to determine the prevalence and susceptibility of extendedspectrum beta – lactamase in urinary isolates of Escherichia coli (E.coli) in a tertiary care hospital, Chennai-South India. A total of 450 urinary isolates of E.coli were collected over a period of six months from April 2008 to September 2008. Antimicrobial susceptibility testing was determined to commonly used antibiotics using the modified Kirby-Bauer’sdisc diffusion method. ESBL detection was done by the screening method of double disc synergy test and then confirmed by the phenotypic confirmatory test with combination disc as recommended by the Clinical Laboratory Standards Institute (CLSI) and the minimuminhibitory concentration (MIC) method using the E test strips (AB Biodisk,Sweden )- as per manufacturer’s instructions. The prevalence of E.coli ESBL was 60%. The ESBL producing isolates were significantly resistant (p < 0.01) to ampicillin, trimethoprim /sulfamethoxazole, norfloxacin and nalidixic acid as compared to non-ESBL producers.Multidrug resistance was significantly (p < 0.01) higher (69%) in ESBL positive isolates than non-ESBL isolates (21%). Knowledge of the prevalence of ESBL and resistance pattern of bacterial isolates in a geographical area will help the clinicians to formulate theguidelines for antibiotic therapy to avoid inappropriate use of extended spectrum cephalosporins

Protein-Protein Interactions in Clathrin Vesicular Assembly: Radial Distribution of Evolutionary Constraints in Interfaces

In eukaryotic organisms clathrin-coated vesicles are instrumental in the processes of endocytosis as well as intracellular protein trafficking. Hence, it is important to understand how these vesicles have evolved across eukaryotes, to carry cargo molecules of varied shapes and sizes. The intricate nature and functional diversity of the vesicles are maintained by numerous interacting protein partners of the vesicle system. However, to delineate functionally important residues participating in protein-protein interactions of the assembly is a daunting task as there are no high-resolution structures of the intact assembly available. The two cryoEM structures closely representing intact assembly were determined at very low resolution and provide positions of Cα atoms alone. In the present study, using the method developed by us earlier, we predict the protein-protein interface residues in clathrin assembly, taking guidance from the available low-resolution structures. The conservation status of these interfaces when investigated across eukaryotes, revealed a radial distribution of evolutionary constraints, i.e., if the members of the clathrin vesicular assembly can be imagined to be arranged in spherical manner, the cargo being at the center and clathrins being at the periphery, the detailed phylogenetic analysis of these members of the assembly indicated high-residue variation in the members of the assembly closer to the cargo while high conservation was noted in clathrins and in other proteins at the periphery of the vesicle. This points to the strategy adopted by the nature to package diverse proteins but transport them through a highly conserved mechanism

Prediction of protein-protein interactions in dengue virus coat proteins guided by low resolution cryoEM structures

<p>Abstract</p> <p>Background</p> <p>Dengue virus along with the other members of the flaviviridae family has reemerged as deadly human pathogens. Understanding the mechanistic details of these infections can be highly rewarding in developing effective antivirals. During maturation of the virus inside the host cell, the coat proteins E and M undergo conformational changes, altering the morphology of the viral coat. However, due to low resolution nature of the available 3-D structures of viral assemblies, the atomic details of these changes are still elusive.</p> <p>Results</p> <p>In the present analysis, starting from Cα positions of low resolution cryo electron microscopic structures the residue level details of protein-protein interaction interfaces of dengue virus coat proteins have been predicted. By comparing the preexisting structures of virus in different phases of life cycle, the changes taking place in these predicted protein-protein interaction interfaces were followed as a function of maturation process of the virus. Besides changing the current notion about the presence of only homodimers in the mature viral coat, the present analysis indicated presence of a proline-rich motif at the protein-protein interaction interface of the coat protein. Investigating the conservation status of these seemingly functionally crucial residues across other members of flaviviridae family enabled dissecting common mechanisms used for infections by these viruses.</p> <p>Conclusions</p> <p>Thus, using computational approach the present analysis has provided better insights into the preexisting low resolution structures of virus assemblies, the findings of which can be made use of in designing effective antivirals against these deadly human pathogens.</p

Shape-dependence of near-field heat transfer between a spheroidal nanoparticle and a flat surface

We study the radiative heat transfer between a spheroidal metallic nanoparticle and a planar metallic sample for near- and far-field distances. In particular, we investigate the shape dependence of the heat transfer in the near-field regime. In comparison with spherical particles, the heat transfer typically varies by factors between 1/2 and 2 when the particle is deformed such that its volume is kept constant. These estimates help to quantify the deviation of the actual heat transfer recorded by a near-field scanning thermal microscope from the value provided by a dipole model which assumes a perfectly spherical sensor

Investigating the KNDy hypothesis in humans by co-administration of kisspeptin, neurokinin B and naltrexone in men

Context: A subpopulation of hypothalamic neurons co-localise three neuropeptides namely kisspeptin, neurokinin B (NKB) and dynorphin collectively termed KNDy neurons. Animal studies suggest they interact to affect pulsatile GnRH release (KNDy hypothesis); kisspeptin stimulates, NKB modulates and dynorphin (an opioid) inhibits. Objective: To investigate the KNDy hypothesis in humans, we assessed for the first time the effects of co-administration of kisspeptin-54, NKB and an opioid receptor antagonist, naltrexone on LH pulsatility (surrogate marker for GnRH pulsatility) and gonadotropin release. Design, setting and participants: Ethically approved prospective, single-blinded placebo-controlled study. Healthy male volunteers (n=5/group) attended our research facility for 8 study visits. Intervention and main outcome measure: After 1h baseline blood sampling, participants received a different intervention at each visit: oral 50mg naltrexone (NAL), 8h intravenous infusions of vehicle, 2.56nmol/kg/h NKB (NKB), 0.1nmol/kg/h kissspeptin-54 (KP) alone and in combination. Frequent blood sampling to measure plasma gonadotropins and sex steroids was conducted and LH pulsatility was determined using blinded deconvolution analysis. Results: All kisspeptin and naltrexone containing groups potently increased LH and LH pulsatility (p<0.001 vs vehicle). NKB alone did not affect gonadotropins. NKB+KP had significantly lower increases in gonadotropins compared with kisspeptin alone (p<0.01). NAL+KP was the only group to significantly increase LH pulse amplitude (p<0.001 vs vehicle). Conclusions: Our results suggest significant interactions between the KNDy neuropeptides on LH pulsatility and gonadotropin release in humans. This has important implications for improving our understanding of GnRH pulse generation in humans

Temperature in nonequilibrium systems with conserved energy

We study a class of nonequilibrium lattice models which describe local redistributions of a globally conserved energy. A particular subclass can be solved analytically, allowing to define a temperature T_{th} along the same lines as in the equilibrium microcanonical ensemble. The fluctuation-dissipation relation is explicitely found to be linear, but its slope differs from the inverse temperature T_{th}^{-1}. A numerical renormalization group procedure suggests that, at a coarse-grained level, all models behave similarly, leading to a two-parameter description of their macroscopic properties.Comment: 4 pages, 1 figure, final versio

Hydrographic features off northeast coast and Andaman - Nicobar Islands in relation to demersal finfish resources

Temperature varied from 17.6 to 28.5°C, salinity values from 32.12 to 35.21 x 10"" and dissolved oxygen from 0.8 to 4.41 ml/1. No identifiable relationship could be established between these three parameters and the total fish abundance, probably because the catch was made up of many species having different requirements. Higher abundance of trawl catches was from January to May when bottom water temperature was relatively low. The highest level of abundance of 2764 kg/hr in February 1989 was recorded when the parameters were 26°C, 33.6 x 10" and 2.71 ml/1; and the lowest level of 43.2 kg/hr in July 1988 was when the parameters were 25.3°C, 34.48 x 10'^ and 1.6 ml/1