Relatório de estágio: gestão e desenvolvimento de pessoas

Versão Final Inclui correções ou alterações sugeridas pelo Júri.O presente relatório tem como objetivo central descrever de forma pormenorizada todas as atividades desenvolvidas ao longo do estágio curricular realizado no âmbito do segundo ano do mestrado de Gestão das Organizações – ramo Gestão de Empresas, na empresa Grupo Ramos Ferreira Engenharia, no período correspondente entre 21 de Outubro de 2019 a 24 de Fevereiro de 2020. Integrado no departamento de Marketing, Qualidade e Pessoas, mais precisamente no subdepartamento de Gestão e Desenvolvimento de Pessoas, a realização do estágio teve como missão central, operacionalizar em todas as linhas de atuação inerentes a este subdepartamento e demais áreas afins. As organizações são continuamente influenciadas por fortes pressões de competitividade, inerentes das constantes mudanças a vários níveis que ocorrem no mercado em que se inserem. Neste sentido, as pessoas que as integram constituem-se como o seu principal recurso na manutenção do sucesso estratégico, privilegiando-se cada vez mais a existência de profissionais talentosos e conhecedores. Assim sendo, a Gestão de Recursos Humanos possui um papel preponderante na definição e implementação de estratégias que visam um conjunto de práticas essenciais a uma adequada gestão e desenvolvimento dos colaboradores de uma organização, de forma alinhada com a estratégia organizacional, para o cumprimento dos objetivos, assim como para a garantia e manutenção de relações interpessoais positivas em contextos laborais, sendo as mesmas reguladas por um conjunto de normas jurídicas. A realização do presente relatório permitiu fundamentar a aprendizagem adquirida ao longo do mestrado com a adquirida e aprofundada em contexto laboral, possibilitando a concretização do estágio curricular realizado, um primeiro contacto com a realidade laboral de áreas integrantes do mestrado e uma aplicação e aquisição prática de conhecimentos, experiência e ferramentas essenciais ao mercado de trabalho.El objetivo central del presente informe es describir en detalle todas las actividades desarrolladas durante la pasantía curricular realizada en el marco del segundo año del Máster en Gestión de las Organizaciones – Gestión de Empresas, en la empresa Grupo Ramos Ferreira Engenharia, en el período correspondiente entre 21 de octubre de 2019 hasta al 24 de febrero de 2020. Integrado en el departamento de Marketing, Calidad y Personas, más precisamente en el subdepartamento de Gestión y Desarrollo de Personas, la pasantía curricular tuvo como misión central, operar en todas las líneas de acción inherentes a este subdepartamento y otras áreas afines. Las organizaciones se ven continuamente influidas por fuertes presiones competitivas, inherentes a los constantes cambios a diversos niveles que se producen en el mercado en el que operan. En este sentido, las personas que las integran son su principal recurso para mantener su éxito estratégico, privilegiando cada vez más la existencia de profesionales talentosos y conocedores. Por consiguiente, la Gestión de Recursos Humanos tiene un papel preponderante en la definición e aplicación de estrategias que apuntan a un conjunto de práticas esenciales para una adecuada gestión y desarrollo de los empleados que integran la organización, en línea con la estrategia de la misma, para el cumplimiento de los objetivos, así como para garantizar y mantener relaciones interpersonales positivas en contextos de trabajo, que se rigen por un conjunto de normas jurídicas. La realización de este informe ha permitido fundamentar los aprendizajes adquiridos durante el máster con los adquiridos y profundizados en el contexto de trabajo, posibilitando la realización de las prácticas curriculares realizadas, un primer contacto con la realidad laboral de las áreas que forman parte del máster y la aplicación y adquisición práctica de conocimientos, experiencia y herramientas esenciales para el mercado laboral.The main purpose of the present report is to describe in thorough detail of all the activities carried throughout the curricular internship in the scope of the second year of the master’s degree in Organizations Management - branch of Business Management, at the company Grupo Ramos Ferreira Engenharia, during the corresponding period between October 21st, 2019 and February 24th, 2020. This internship was integrated in the department of Marketing, Quality and People, more precisely in the subdepartment of Management and People Development. The main purpose of this internship was to act in the lines of operation within the subdepartment and all of its underlying areas. Organizations are continuously influenced by strong competitive pressures, inherent to the constant changes at various levels that occur in the market in which they operate. So, following this line of thought, the people that integrate said organizations are the key to its success as they become the company's main resource in maintaining strategic success, favouring the presence of increasingly talented and knowledgeable individuals. Therefore, Human Resource Management has a crucial role in the implementation of strategies that aim at a set of practices essential to promote an adequate level of management and development of the organization’s employees, in line with the organization’s strategy, to fulfill its objectives as well as ensure and maintain positive interpersonal relationships in work contexts, which are regulated by a set of legal norms. The execution of this report allowed for the consolidation of the knowledge acquired throughout the course, deepening the understanding of said knowledge in a work environment. This internship made it possible to have a first contact with the reality of this market and the areas related to the course, also allowing the acquisition of experience, knowledge, and tools essential to integrate the working market

The making of multilateralist germany implications for US-german bilateral relations

“Em Novembro de 1989, o mundo assistia extasiado à queda do muro de Berlim. O simbolismo do acontecimento não se perdeu à medida que alemães orientais e ocidentais quebravam a materialização em cimento do que Winston Churchill anos antes chamara a “Cortina de Ferro”. A queda do muro de Berlim e consequente processo de reunificação, acompanhado pelo fim das divisões da Guerra Fria que rodeavam a Europa, criou mudanças tectónicas na geopolítica global. O processo de reunificação alemão, acompanhado por um espírito internacional de cooperação e optimismo, pendendo para a euforia, forneceu um modelo inspirador de diplomacia positiva e popular autodeterminação trabalhando conjuntamente para um bem comum – era uma diplomacia por excelência. Diplomatas e políticos em Washington, Moscovo, Londres e Paris lutaram para manter os acontecimentos pacíficos na Europa central e todo o processo de reunificação foi marcado por um espírito notável de internacionalismo; (...) Esse espírito de colaboração transatlântica contrasta com a situação uma década e meia mais tarde. Desde do final de 2002, muito foi feito no cada vez maior distanciamento cultural e político entre EUA e Europa. Este distanciamento assenta firmemente onde política externa e valores culturais convergem. O historiador britânico Timothy Garton Ash chamou-lhe “a crise do ocidente, a mais profunda desde do fim da Guerra Fria”

Carbamazepine inhibits angiotensin I-converting enzyme, linking it to the pathogenesis of temporal lobe epilepsy

We find that a common mutation that increases angiotensin I-converting enzyme activity occurs with higher frequency in male patients suffering from refractory temporal lobe epilepsy. However, in their brains, the activity of the enzyme is downregulated. As an explanation, we surprisingly find that carbamazepine, commonly used to treat epilepsy, is an inhibitor of the enzyme, thus providing a direct link between epilepsy and the renin-angiotensin and kallikrein-kinin systems. Translational Psychiatry (2012) 2, e93; doi:10.1038/tp.2012.21; published online 13 March 2012INNTConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Biophys, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023032 São Paulo, BrazilUniv São Paulo, Sch Arts Sci & Humanities, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Sci & Technol, BR-04023032 São Paulo, BrazilNove de Julho Univ UNINOVE, Dept Rehabil Sci, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Sci & Technol, BR-04023032 São Paulo, BrazilWeb of Scienc

Central nervous-system kinin receptors and the hypertensive response mediated by bradykinin

1 Bradykinin (Bk) administered intracerebroventricularly to the rat causes an increase in arterial pressure. 2 Analogues of Bk with agonist and antagonist activity were injected, over a wide dose‐range, into the posterior region of the fourth ventricle of unanaesthetized rats implanted with permanent ventricular canullae, and blood pressure was measured directly from the abdominal aorta. 3 The analogues Ile‐Ser‐Bk (T‐kinin) and Lys‐Lys‐Bk, which interact with both B1 and B2 Bk receptors, produced pressor effects similar to those of Bk, although of greater duration, whereas des‐Arg9‐Bk, a B1 ‐receptor agonist, had no effect. 4 The B1‐antagonist des‐Arg9‐[Leu8]‐Bk did not alter the Bk pressor response, but D‐Arg‐[Hyp3, Thi5,8,D‐Phe7]‐Bk, which interacts both with B1‐ and B2‐receptors blocked the responses to Bk, T‐kinin and Lys‐Lys‐Bk and caused parallel shifts to the right of the Bk dose‐response curves. Neither antagonist, by itself, had any effect on blood pressure. 5 It is concluded that the central pressor response to Bk is mediated by receptors of the B2 subtype.ESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034 SAO PAULO,SP,BRAZILESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034 SAO PAULO,SP,BRAZILWeb of Scienc

Analogues containing the paramagnetic amino acid TOAC as substrates for angiotensin 1-converting enzyme

The angiotensin I-converting enzyme (ACE) converts the decapeptide angiotensin I (Ang I) into angiotensin II by releasing the C-terminal dipeptide. A novel approach combining enzymatic and electron paramagnetic resonance (EPR) studies was developed to determine the enzyme effect on Ang I containing the paramagnetic 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) at positions 1, 3, 8, and 9. Biological assays indicated that TOAC(1)-Ang I maintained partly the Ang I activity, and that only this derivative and the TOAC(3)-Ang I were cleaved by ACE. Quenching of Tyr(4) fluorescence by TOAC decreased with increasing distance between both residues, suggesting an overall partially extended structure. However, the local bend known to be imposed by the substituted diglycine TOAC is probably responsible for steric hindrance, not allowing the analogues containing TOAC at positions 8 and 9 to act as substrates. in some cases, although substrates and products differ by only two residues, the difference between their EPR spectral lineshapes allows monitoring the enzymatic reaction as a function of time. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

Role of the second disulfide bridge (Cys(18)-Cys(274)) in stabilizing the inactive AT(1) receptor

Previous research showed that disruption of the Cys(18)-Cys(274) bond in the angiotensin II (AngII) AT(1) receptor mutant (C18S), expressed in CHO cells, causes an increase in the basal activity and attenuation of the maximum response to AngII. in addition, this mutant was mostly intracellularly distributed. Our aim was to investigate whether the intracellular presence of the mutant was due to a constitutive internalization or to a defective maturation of the receptor. the first hypothesis was assessed by pretreating the cells with losartan or [Sar(1)Leu(8)]-AngII, specific AT(1) receptor antagonists, a maneuver to revert the receptor internalization. the second hypothesis was tested using calnexin, an endoplasmic reticulum marker. We found that treatment with AT(1) receptor antagonists causes an increase in the binding ability of the mutant to AngII. Furthermore, whereas the maximum effect is increased, it reduces the enhanced basal levels of IP3. the hypothesis for a lack of maturation of the mutant receptor was ruled out because calnexin was poorly colocalized with the intracellular C18S receptor. Our results suggest that the mutation of the AT(1) receptor leads to a conformational structure similar to that of the active mode of the AT(1) receptor, favoring its internalization in the absence of the agonist.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Biophys, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilFAPESP: 07/01910-0Web of Scienc

Leptin regulates ACE activity in mice

Leptin is a hormone related to metabolism. It also influences blood pressure, but the mechanisms triggered in this process are not yet elucidated. Angiotensin-I converting enzyme (ACE) regulates cardiovascular functions and recently has been associated with metabolism control and obesity. Here, we used ob/ob mice, a model lacking leptin, to answer the question whether ACE and leptin could interact to influence blood pressure, thereby linking the renin-angiotensin system and obesity. These mice are obese and diabetic but have normal 24 h mean arterial pressure. Our results show that plasma and lung ACE activities as well as ACE mRNA expression were significantly decreased in ob/ob mice. in agreement with these findings, the hypotensive effect produced by enalapril administration was attenuated in the obese mice. Plasma renin, angiotensinogen, angiotensin I, bradykinin, and angiotensin 1-7 were increased, whereas plasma angiotensin II concentration was unchanged in obese mice. Chronic infusion of leptin increased renin activity and angiotensin II concentration in both groups and increased ACE activity in ob/ob mice. Acute leptin infusion restored ACE activity in leptin-deficient mice. Moreover, the effect of an ACE inhibitor on blood pressure was not changed in ob/+ mice during leptin treatment but increased four times in obese mice. in summary, our findings show that the renin-angiotensin system is altered in ob/ob mice, with markedly reduced ACE activity, which suggests a possible connection between the renin-angiotensin system and leptin. These results point to an important interplay between the angiotensinergic and the leptinergic systems, which may play a role in the pathogenesis of obesity, hypertension, and metabolic syndrome.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Deutsche Akademische Austauschdienst (DAAD/PROBRAL)Deutsche ForschungsgemeinschaftUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, BrazilMax Delbruck Ctr Mol Med MDC, ECRC, Berlin, GermanyUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, BR-04023062 São Paulo, BrazilDeutsche Forschungsgemeinschaft: BA 1374/16-1Web of Scienc