Učestalost supkliničkog endometritisa u krava koje se pregone te usporedba učinkovitosti različite antibiotske i biljne intrauterine terapije

The present research work was conducted to investigate the incidence of subclinical endometritis (SE) in repeat breeder dairy cattle, and to study the comparative efficacy of different antimicrobial and polyherbal intrauterine therapies. Repeat breeding crossbred cows (>90 days in milking) with clear cervico-vaginal mucous discharge in spontaneous estrus were examined for uterine cytology by the cytobrush technique. The cows with ≥ 4% polymorphonuclear cells (PMNs) in uterine cytology were considered to be affected with SE. These cows were randomly and equally divided (n=12/group) into 5 groups and treated with the intrauterine medicine Vodine-IU (T1), Metricare-IU (T2), Utriguard-IU (T3), Uraksha (polyherbal preparation) (T4), or left untreated (control/C). In the subsequent estrus, uterine cytology was repeated and the cows were inseminated. The cows that failed to conceive but showed estrus were re-inseminated. The pregnancy status was checked. The incidence of SE was observed to be 36.80% (60/163). The PMNs percentage significantly declined in all the treatment group cows in response to treatment. The highest conception rate was observed in the T4 group (Uraksha treated) cows. It was concluded that the uterine cytobrush technique can be used efficiently for diagnosis of SE in dairy cattle, and polyherbal intrauterine drugs may be a good alternative in intrauterine antimicrobial therapy.Rad je proveden kako bi se istražila učestalost supkliničkog endometritisa (SE) u mliječnih krava koje se pregone. Također, usporedila se učinkovitost različitih antimikrobnih i poliherbalnih intrauterinih terapijskih postupaka. U krava koje se pregone (više od 90 dana u mužnji), s čistim cerviko-vaginalnim iscjetkom iz rodnice u spontanom estrusu, tehnikom citočetkice učinjena je citološka pretraga maternice. Za krave kod kojih je utvrđeno 4% polimorfonuklearnih stanica (PMN) smatralo se da imaju supklinički endometritis. Životinje su nasumično podijeljene u pet skupina s jednakim brojem jedinki (n=12 po skupini) i liječene intrauterino sljedećim lijekovima: Vodine (skupina T1), Metricare (skupina T2), Utriguard (skupina T3), Uraksha (poliherbalni pripravak; skupina T4), dok je peta skupina bila kontrolna skupina (C). U sljedećem estrusu ponovljena je citološka analiza maternice i krave su osjemenjene. Krave nisu ostale gravidne te su u sljedećem estrusu ponovno osjemenjene. Provjeren je status gravidnosti. Incidencija SE-a bila je 36,80 % (60/163). Postotak PMN-a znatno se smanjio u svih krava u tretiranim skupinama kao odgovor na liječenje. Najveća stopa koncepcije zabilježena je u krava u skupini T4 (liječene Urakshom). Zaključeno je da tehnika citočetkica može učinkovito poslužiti u dijagnostici SE-a u mliječnih goveda te da poliherbalni intrauterini lijekovi mogu biti dobra alternativa antimikrobnoj intrauterinoj terapiji

The surged faradic stimulation to the pelvic floor muscles as an adjunct to the medical management in children with rectal prolapse

<p>Abstract</p> <p>Background</p> <p>To assess the role of the surged faradic stimulation to the pelvic floor muscles as an adjunct to the conservative management in the children of idiopathic rectal prolapse</p> <p>Methods</p> <p><it>Study design</it>: Prospective</p> <p><it>Setting</it>: Pediatric Surgery Department, Pt BD Sharma, Post Graduate Institute of Medical Sciences, Rohtak</p> <p><it>Subjects</it>: 47 consecutive children with idiopathic rectal prolapse attending the Pediatric Surgery out patient department from July 2005 to June 2006</p> <p><it>Methodology</it>: The information pertaining to duration and the extent of rectal prolapse, predisposing or associated medical conditions, results of local clinical examination were noted. Surged faradic stimulation using modified intraluminal rectal probe, was given on the alternate days. The conventional conservative medical management was also continued. The extent of relief and the number of the sittings of faradic stimulation required were noted at various stages of follow-ups</p> <p><it>Statistical Methods</it>: Mean values between those completely cured and others; poor responders and others were compared with t-test and proportions were compared with Chi square test. The p-value < 0.05 was considered statistically significant.</p> <p>Results</p> <p>The mean number of sittings in the completely cured group (n = <b>28</b>(64%)) was (12.4 ± 7.8) and was comparable with very poor responder (n = 6(13%). There was higher percentage of relief (76%) at the first follow up (at 15 days) in completely cured Vs other (37%) and also the poor responders showed (20%) Vs other (68%) and was statistically significant.</p> <p>Conclusion</p> <p>With use of faradic stimulation, even the long-standing rectal prolapse can be fully cured. The follow up visit at 2 weeks is very important to gauge the likely success of this modality in treatment of the patients with rectal prolapse. Those showing poor response at this stage may require alternative treatment or take a long time to get cured</p

CPP-ZFN: A potential DNA-targeting anti-malarial drug

<p>Abstract</p> <p>Background</p> <p>Multidrug-resistant <it>Plasmodium </it>is of major concern today. Effective vaccines or successful applications of RNAi-based strategies for the treatment of malaria are currently unavailable. An unexplored area in the field of malaria research is the development of DNA-targeting drugs that can specifically interact with parasitic DNA and introduce deleterious changes, leading to loss of vital genome function and parasite death.</p> <p>Presentation of the hypothesis</p> <p>Advances in the development of zinc finger nuclease (ZFN) with engineered DNA recognition domains allow us to design and develop nuclease of high target sequence specificity with a mega recognition site that typically occurs only once in the genome. Moreover, cell-penetrating peptides (CPP) can cross the cell plasma membrane and deliver conjugated protein, nucleic acid, or any other cargo to the cytoplasm, nucleus, or mitochondria. This article proposes that a drug from the combination of the CPP and ZFN systems can effectively enter the intracellular parasite, introduce deleterious changes in its genome, and eliminate the parasite from the infected cells.</p> <p>Testing the hypothesis</p> <p>Availability of a DNA-binding motif for more than 45 triplets and its modular nature, with freedom to change number of fingers in a ZFN, makes development of customized ZFN against diverse target DNA sequence of any gene feasible. Since the <it>Plasmodium </it>genome is highly AT rich, there is considerable sequence site diversity even for the structurally and functionally conserved enzymes between <it>Plasmodium </it>and humans. CPP can be used to deliver ZFN to the intracellular nucleus of the parasite. Signal-peptide-based heterologous protein translocation to <it>Plasmodium</it>-infected RBCs (iRBCs) and different <it>Plasmodium </it>organelles have been achieved. With successful fusion of CPP with mitochondrial- and nuclear-targeting peptides, fusion of CPP with 1 more <it>Plasmodium </it>cell membrane translocation peptide seems achievable.</p> <p>Implications of the hypothesis</p> <p>Targeting of the <it>Plasmodium </it>genome using ZFN has great potential for the development of anti-malarial drugs. It allows the development of a single drug against all malarial infections, including multidrug-resistant strains. Availability of multiple ZFN target sites in a single gene will provide alternative drug target sites to combat the development of resistance in the future.</p

Dynamic control of a single-server system with abandonments

In this paper, we discuss the dynamic server control in a two-class service system with abandonments. Two models are considered. In the first case, rewards are received upon service completion, and there are no abandonment costs (other than the lost opportunity to gain rewards). In the second, holding costs per customer per unit time are accrued, and each abandonment involves a fixed cost. Both cases are considered under the discounted or average reward/cost criterion. These are extensions of the classic scheduling question (without abandonments) where it is well known that simple priority rules hold. The contributions in this paper are twofold. First, we show that the classic c-μ rule does not hold in general. An added condition on the ordering of the abandonment rates is sufficient to recover the priority rule. Counterexamples show that this condition is not necessary, but when it is violated, significant loss can occur. In the reward case, we show that the decision involves an intuitive tradeoff between getting more rewards and avoiding idling. Secondly, we note that traditional solution techniques are not directly applicable. Since customers may leave in between services, an interchange argument cannot be applied. Since the abandonment rates are unbounded we cannot apply uniformization-and thus cannot use the usual discrete-time Markov decision process techniques. After formulating the problem as a continuous-time Markov decision process (CTMDP), we use sample path arguments in the reward case and a savvy use of truncation in the holding cost case to yield the results. As far as we know, this is the first time that either have been used in conjunction with the CTMDP to show structure in a queueing control problem. The insights made in each model are supported by a detailed numerical study. © 2010 Springer Science+Business Media, LLC

Influenza Virus A Infection of Human Monocyte and Macrophage Subpopulations Reveals Increased Susceptibility Associated with Cell Differentiation

Influenza virus infection accounts for significant morbidity and mortality world-wide. Interactions of the virus with host cells, particularly those of the macrophage lineage, are thought to contribute to various pathological changes associated with poor patient outcome. Development of new strategies to treat disease therefore requires a detailed understanding of the impact of virus infection upon cellular responses. Here we report that human blood-derived monocytes could be readily infected with the H3N2 influenza virus A/Udorn/72 (Udorn), irrespective of their phenotype (CD14++/CD16−, CD14++/CD16+ or CD14dimCD16++), as determined by multi-colour flow cytometry for viral haemagglutinin (HA) expression and cell surface markers 8–16 hours post infection. Monocytes are relatively resistant to influenza-induced cell death early in infection, as approximately 20% of cells showed influenza-induced caspase-dependent apoptosis. Infection of monocytes with Udorn also induced the release of IL-6, IL-8, TNFα and IP-10, suggesting that NS1 protein of Udorn does not (effectively) inhibit this host defence response in human monocytes. Comparative analysis of human monocyte-derived macrophages (Mph) demonstrated greater susceptibility to human influenza virus than monocytes, with the majority of both pro-inflammatory Mph1 and anti-inflammatory/regulatory Mph2 cells expressing viral HA after infection with Udorn. Influenza infection of macrophages also induced cytokine and chemokine production. However, both Mph1 and Mph2 phenotypes released comparable amounts of TNFα, IL-12p40 and IP-10 after infection with H3N2, in marked contrast to differential responses to LPS-stimulation. In addition, we found that influenza virus infection augmented the capacity of poorly phagocytic Mph1 cells to phagocytose apoptotic cells by a mechanism that was independent of either IL-10 or the Mer receptor tyrosine kinase/Protein S pathway. In summary, our data reveal that influenza virus infection of human macrophages causes functional alterations that may impact on the process of resolution of inflammation, with implications for viral clearance and lung pathology

Event-by-event correlations between Λ\Lambda (Λˉ\bar{\Lambda}) hyperon global polarization and handedness with charged hadron azimuthal separation in Au+Au collisions at sNN=27 GeV\sqrt{s_{\text{NN}}} = 27 \text{ GeV} from STAR

Global polarizations ($P$) of $\Lambda$ ($\bar{\Lambda}$) hyperons have been observed in non-central heavy-ion collisions. The strong magnetic field primarily created by the spectator protons in such collisions would split the $\Lambda$ and $\bar{\Lambda}$ global polarizations ($\Delta P = P_{\Lambda} - P_{\bar{\Lambda}} < 0$). Additionally, quantum chromodynamics (QCD) predicts topological charge fluctuations in vacuum, resulting in a chirality imbalance or parity violation in a local domain. This would give rise to an imbalance ($\Delta n = \frac{N_{\text{L}} - N_{\text{R}}}{\langle N_{\text{L}} + N_{\text{R}} \rangle} \neq 0$) between left- and right-handed $\Lambda$ ($\bar{\Lambda}$) as well as a charge separation along the magnetic field, referred to as the chiral magnetic effect (CME). This charge separation can be characterized by the parity-even azimuthal correlator ($\Delta\gamma$) and parity-odd azimuthal harmonic observable ($\Delta a_{1}$). Measurements of $\Delta P$, $\Delta\gamma$, and $\Delta a_{1}$ have not led to definitive conclusions concerning the CME or the magnetic field, and $\Delta n$ has not been measured previously. Correlations among these observables may reveal new insights. This paper reports measurements of correlation between $\Delta n$ and $\Delta a_{1}$, which is sensitive to chirality fluctuations, and correlation between $\Delta P$ and $\Delta\gamma$ sensitive to magnetic field in Au+Au collisions at 27 GeV. For both measurements, no correlations have been observed beyond statistical fluctuations.Comment: 10 pages, 10 figures; paper from the STAR Collaboratio

Hyperon polarization along the beam direction relative to the second and third harmonic event planes in isobar collisions at sNN\sqrt{s_{NN}} = 200 GeV

The polarization of $\Lambda$ and $\bar{\Lambda}$ hyperons along the beam direction has been measured relative to the second and third harmonic event planes in isobar Ru+Ru and Zr+Zr collisions at $\sqrt{s_{NN}}$ = 200 GeV. This is the first experimental evidence of the hyperon polarization by the triangular flow originating from the initial density fluctuations. The amplitudes of the sine modulation for the second and third harmonic results are comparable in magnitude, increase from central to peripheral collisions, and show a mild $p_T$ dependence. The azimuthal angle dependence of the polarization follows the vorticity pattern expected due to elliptic and triangular anisotropic flow, and qualitatively disagree with most hydrodynamic model calculations based on thermal vorticity and shear induced contributions. The model results based on one of existing implementations of the shear contribution lead to a correct azimuthal angle dependence, but predict centrality and $p_T$ dependence that still disagree with experimental measurements. Thus, our results provide stringent constraints on the thermal vorticity and shear-induced contributions to hyperon polarization. Comparison to previous measurements at RHIC and the LHC for the second-order harmonic results shows little dependence on the collision system size and collision energy.Comment: 6 pages, 5 figures, Published in Physical Review Letter