BANGO SEA XLOC/HMBC-H2OBC: complete heteronuclear correlation within minutes from one NMR pulse sequence

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Herpes simplex virus PCR in 2230 explanted corneal buttons

Purpose: To determine herpes simplex virus (HSV) DNA prevalence and mean cycle threshold of polymerase chain reaction (PCR) in corneal tissue of patients with penetrating keratoplasty (PKP), with (HSK+) and without (HSK−) previous clinical herpetic keratitis history. Methods: Retrospective review of recipient corneal buttons which were explanted through PKP between March 2010 and September 2018 at the Department of Ophthalmology, Saarland University Medical Center in Homburg/Saar, Germany. Corneal tissue samples were analysed by real-time PCR for the presence of HSV DNA. For each subject, clinical data, including patients’ demographics and clinical diagnoses, were collected. Results: In total, 2230 corneal samples (age at the time of the surgery 57.3 19.2 years) of 1860 patients were analysed. HSV PCR was positive in 137 (6.1%) corneal samples, with a 30.57 6.01 (range 14–39) mean cycle threshold (Ct) value. Two hundred ninety-eight (13.4%) corneas of 266 patients were clinically HSK+, and 1932 (86.6%) corneas of 1600 patients were clinically HSK−. HSV DNA was detected significantly more frequently (p < 0.0001) in HSK+ corneal samples (108 corneal samples; 36.2%), than in HSK− corneal samples (29 corneal samples; 1.5%). Ct value was significantly lower in HSK+ than in HSK- corneal samples (29.8 5.8 versus 32.6 5.9; p = 0.008). Conclusion: Our data demonstrate that a positive clinical history of HSK is related to HSV PCR positivity in about every 2.8th patient. In addition, about every 66th explanted corneal tissue is HSV PCR-positive despite the lack of clinical suspicion. These patients may need additional local/systemic antiviral treatment to avoid newly acquired HSK following penetrating keratoplasty

Clinicopathological Review of 547 Bulbar Enucleations in Hungary (2006–2017)

Purpose. To analyse current clinicopathological enucleation indications in a large third-referral centre in a developed country (Hungary) over a period of 12 years. Methods. Retrospective review was performed on 547 enucleated eyes of 543 patients (48.6% males, age 52.7 ± 24.5 years) who were operated on between 2006 and 2017 at the Department of Ophthalmology of Semmelweis University, in Budapest, Hungary. For each subject, clinicopathological data, including patient demographics, indications for enucleation, B-scan ultrasound reports, operative details, and histopathological analyses, were reviewed. Primary enucleation indications were classified into trauma, tumours, systemic diseases, surgical diseases, infections or inflammations, miscellaneous diseases, and unclassifiable groups. Clinical immediate enucleation indications were classified as tumours, atrophia or phthisis bulbi, infection or inflammation, painful blind eye due to glaucoma, acute trauma, threatening or spontaneous perforation, cosmetic causes, and expulsive bleeding. Results. The most common primary enucleation indications were tumours (47.3%), trauma (16.8%), surgical diseases (15.7%), infection or inflammation (11.6%), systemic diseases (5.1%), miscellaneous diseases (2.0%), and unclassifiable diseases (1.5%). Clinical immediate enucleation indications were tumours (46.1%), atrophia or phthisis bulbi (18.5%), infection or inflammation (18.5%), painful blind eye due to glaucoma (11.2%), acute trauma (3.7%), threatening or spontaneous perforation (1.3%), cosmetic reasons (0.5%), and expulsive bleeding (0.4%). Conclusions. Intraocular tumours represent the most common clinicopathological indication for ocular enucleation in our study population. Following ocular trauma and systemic diseases, the rate of enucleation decreased in the last decade, compared to those previously reported in other developed countries. However, changes were not observed for surgical diseases, infectious and inflammatory causes, or for miscellaneous and unclassified diseases. Orbital implant financing should be increased to ensure better postoperative aesthetic rehabilitation, following enucleation in Hungary

Véletlen jelenségek térben és időben = Randomness in Space and Time

A statissztikus fizika és kvantummechanika matematikai problémáival foglalkozunk. A hidrodinamika mikroszkopikus modelljeinek tág osztályait vizsgáltuk, különös tekintettel hiperbolikus skálázású rendszerek makroszkopikus viselkedésére a lökéshulllámok tartományában. Levezettük a rugalmasságtan nemlineáris egyenleteit. Áttörést jelentő eredmények születtek aszimmetrikus modellek 1/3 kitevős skálázásával kapcsolatban (szubdiffúzív viselkedés). Erdős-Rényi-Barabási tipusú ömszervező strukturák kritikus viselkedését, a gráf komponenseinek aszimptokus méretét tisztáztuk. Nem-Markov bolyongások határeloszlását meghatározva meglepő különbséget tapasztaltunk az irányított és nem irányított élek eseteinél. Leírtuk véletlen mátrixok sajátértékeinek pomtfolyamatát. Sinai biliárdok és általánosabb kaotikus dinamikai rendszerek ergodikus viselkedését tisztáztuk, eredményesen tárgyaltuk a sikbeli Lorentz folyamat rekurrenciájának és Brown-közelítésének problémáját. Meghatároztuk iterált függvényrendszerek attraktorainak Hausdorff dimnenzióját, véletlen Cantor halmazok különbségét. Tisztáztuk a kvantummechanikai állapottér geometriájának kérdéseit. A kvantumrendszerek állapotbecsléseit vizsgálva azok megbízhatóságát jellemeztük. Algoritmust adtunk n-szintű rendszer "constrained" becsléseire, a becslési stratégia számítógépes vizsgálatára is sor került. A folytonos optimalizáció különféle algoritmusit konstruáltuk meg, tisztáztuk azok hatékonyságát. | Mathematical problems of statistical physics and quantum mechanics are investigated. Various microscopic models of hydrodynamics are introduced, existence of hyperbolic scaling limits is prroven including the derivation of the equations of nonlinear elastodynamics. Fairly deep results have been obtained on the 1/3 exponent scaling of asymmetric systems (subdiffusive behaviour of the tagged particle). In case of Erdős-Rényi-Barabási type self-organized systems the size of the graph components has been determined. Investigating the limit distribution of non-Markovian random walks, a considerable difference in the behavior of models with directed and non-directed bonds has been observed. Characterization of the point process of eigenvalues of random matrices was presented. Ergodic behavior of Sinai billiards and of more general dynamical systems has been described, discussion of recurrence and Brownian approximation of the planar Lorentz gas has been completed. Hausdorff dimension of attractors of iterated maps has been calculated, difference of random Cantor sets was also studied. Geometry of quantum mechanical and reliability of estimators for the state of quantum mechanical systems has been investigated. Algorithms for constrained estimation for n-level quantum systems have been introduced and investigated by computer simulations. Various methods of continuous optimalization and their effectiveness was also studied

Photochemical and Structural Studies on Cyclic Peptide Models

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Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis

: During muscle cell differentiation, the alternatively spliced, acidic β-domain potentiates transcription of Myocyte-specific Enhancer Factor 2 (Mef2D). Sequence analysis by the FuzDrop method indicates that the β-domain can serve as an interaction element for Mef2D higher-order assembly. In accord, we observed Mef2D mobile nuclear condensates in C2C12 cells, similar to those formed through liquid-liquid phase separation. In addition, we found Mef2D solid-like aggregates in the cytosol, the presence of which correlated with higher transcriptional activity. In parallel, we observed a progress in the early phase of myotube development, and higher MyoD and desmin expression. In accord with our predictions, the formation of aggregates was promoted by rigid β-domain variants, as well as by a disordered β-domain variant, capable of switching between liquid-like and solid-like higher-order states. Along these lines, NMR and molecular dynamics simulations corroborated that the β-domain can sample both ordered and disordered interactions leading to compact and extended conformations. These results suggest that β-domain fine-tunes Mef2D higher-order assembly to the cellular context, which provides a platform for myogenic regulatory factors and the transcriptional apparatus during the developmental process