An Observational study to evaluate the effect of Vaitarana Basti in the management of Vatakaphaja Gridhrasi vis-à-vis Sciatica

Sciatica refers to the low back pain radiating to lower limb in a dermatomal distribution. Sciatica is a common cause of pain and disability; it is more persistent and severe than low back pain. Sciatica prevalence from different studies ranged from 1.2% to 43%. Physical activity at work and occupational workload, such as lifting, work related twisting of the trunk, occupational exposure to whole body vibration for example machine operators, motor vehicle drivers have also been suggested to be risk factors for Sciatica. Obesity has previously been linked to Sciatica. All these factors create pressure on spinal cord producing low back ache and radiating pain. Signs and Symptoms of Sciatica shows close resemblance with Gridhrasi. In Ayurveda, Acharya Vangasena has mentioned Vaitarana Basti in the management of Gridhrasi. Vaitarana Basti adopted here in this study has a direct reference for Gridhrasi in Vangasena samhita and Acharya Chakradutta while mentioning Vaitarana Basti has indicated it in Shula, Vatakaphaja vikaras. Considering the ingredients, it is a Shodhana, Teekshna type of Basti which is beneficial in Vatakaphaja Gridhrasi. A minimum of 20 subjects who fulfilled the diagnostic and inclusion criteria were subjected to the intervention. The overall results in the study revealed statistically highly significant result

First Report of Fusarium proliferatum Causing Rot of Onion Bulbs (Allium cepa L.) in India

A rot disease was observed on onion bulbs in major growing areas of Kadapa and Kurnool districts of Andhra Pradesh, India during 2010 to 2012. Based on pathogenicity, morphology and ribosomal DNA spacer sequences, the pathogen was identified as Fusarium proliferatum (Matsushima) Nirenberg. The fungus was isolated from onion bulbs presenting purple and reddish lesions, obtaining F. proliferatum consistently. The fungus produced effuse white colonies, branched hyphae, short conidiophores, slightly curved macroconidia, and single celled microconidia measuring 5.6-10.5 X 2.0-3.5 μm in diameter. Morphological identification of the fungus was confirmed using ribosomal DNA sequence data. Kotch’s postulates were confirmed by performing pathogenicity test on healthy onion bulbs. This is the first report of F. proliferatum causing rot disease on onion bulbs in India; although it had already been reported for onion in the USA and Serbia.Keywords: Onion bulbs; Rot disease; Fusarium proliferatum; Pathogenicity; rDNA - IT

PREDICTING HEART DISEASE USING DATAMINING TECHNIQUES

Nowadays, hardihood disorder is escalating regularly by virtue of life taste, transmitted. Especially, mind disorder has develop into more popular the above-mentioned days.i.e. life of folk persecute risk.Everyone has strange beliefs for Blood influence, cholesterol and vital sign. But in line with medically proven results the healthy beliefs of Blood influence is 120/90, cholesterol is and temperature is 72. This essay gives the evaluate around extraordinary coordination techniques used for predicting the risk matched of anybody occupying on age, feminine, Blood constraint, cholesterol, vital sign. The inmate risk flatten is restricted practicing datamining allotment techniques being Naïve Bayes, KNN, Decision Tree Algorithm, Neural Network. etc., Accuracy of the risk matched is high when adopting more estimate of attributes

Mechanism of haematotoxicity induced by phenylhydrazine: a review

This work was carried out to show the effects of phenylhydrazine (PHZ) induced anaemic condition. Anaemic condition is defined as reduction in red blood cells (RBC) than normal number of red blood cells. The anti-anaemic activity can be studied using the changes in haematological parameters (PCV, RBC & Haemoglobin) influenced by PHZ [(40mg/kg p.o.)] in rats. PHZ, a potent chemical that causes different effects on different tissues at several levels. Administration of PHZ causes haemolytic anaemia, genotoxic effects and rose in iron absorption in spleen, liver and duodenum & causes change in iron metabolism. PHZ acts by activating immune response which triggers phagocytosis and also interfere with the binding of erythropoietin (EPO) receptors and further JAK-STAT pathway. PHZ also causes genotoxic effect by forming single strand DNA damage. In view of lipid peroxidation along with the formation of Thiobarbituric acid (TBA)-reactive malonyldialdehyde, it is recommend that PHZ induces anaemia as an outcome of peroxidation of  RBC membrane lipids and this effect may be a upshot of the autoxidation of the drug and the interaction of membrane lipids and oxygen radical

Effect of Feeding Differently Processed Sweet Sorghum (Sorghum bicolor L. Moench) Bagasse Based complete Diet on Nutrient Utilization and Microbial N Supply in Growing Ram Lambs

This study was carried out to identify appropriate processing method for efficient utilization of sweet sorghum bagasse (SSB), an agro-industrial by product of ethanol industry after blending with concentrate. SSB based complete diet with roughage to concentrate ratio of 50:50 was processed into mash (SSBM), expander extruded pellet (SSBP), chop form (SSBC) and evaluated in comparison to sorghum stover based complete diet in mash form (SSM). Twenty four Nellore X Deccani ram lambs (9 month age; 21.1 ± 0.57 kg body weight) were randomly divided into four groups of six animals each and the experimental complete diets were allotted at random to each group and evaluated for their intake, nutrient utilization and microbial N supply. Among all the groups, the average dry matter (DM) intake (g/kg w0.75), digested DM, organic matter and crude protein were higher (P < 0.01) in lambs fed SSBP diet. The cellulose digestibility was higher (P < 0.05) in lambs fed SSBP diet than those fed SSM and SSBC diets. Intake of digestible crude protein (DCP, g/d) and metabolizable energy (MJ/d) were higher (P < 0.01) in lambs fed SSBP diet. The SSBP diet had higher (P < 0.01) DCP and N (P < 0.05) balance compared to other three diets. Increased (P < 0.01) purine derivatives and microbial N supply was observed in processed diets. Expander extrusion of SSB based complete diet resulted in improved (P < 0.01) efficiency of microbial protein synthesis. It is concluded that, when SSB was processed into complete diets, in terms of nutrient utilization and microbial N supply, the expander extruded pellet diet was better utilized than chopped or mash form by the growing ram lambs

Growth performance and carcass characteristics of growing ram lambs fed sweet sorghum bagasse-based complete rations varying in roughage-to-concentrate ratios

Different roughage-to-concentrate ratios of sweet sorghum bagasse (SSB) (a by-product of the biofuel industry)-based complete diets were assessed. Twenty four growing Nellore × Deccani ram lambs aged about 3 months (average body wt., 10.62 ± 0.25 kg) were randomly allotted to four complete rations (CR) varying in roughage-to-concentrate ratios viz. 60:40 (CR-I), 50:50 (CR-II), 40:60 (CR-III) and 30:70(CR-IV) for a period of 180 days. The feed intake was comparable among the lambs fed different experimental complete diets. Average daily weight gain (in grams) was 77.31 ± 4.90, 81.76 ± 5.16, 85.83 ± 2.83 and 86.30 ± 3.25, and feed conversion ratio (in kilograms of feed per kilogram gain) averaged 11.42 ± 0.68, 10.57 ± 0.64, 10.17 ± 0.37 and 9.96 ± 0.38 in ram lambs fed CR-I, CR-II, CR-III and CR-IV rations, respectively. Statistically, differences in daily weight gain and feed conversion ratio among the lambs fed four experimental rations were not significant (P > 0.05). The cost per kilogram gain was significantly (P < 0.01) higher in ram lambs fed CR-IV and CR-III rations compared to CR-I ration, and it was comparable between CR-I and CR-II rations. Dressing percentage averaged 44.90 ± 0.15, 42.57 ± 0.72, 43.67 ± 0.16 and 44.42 ± 0.76 for the respective diets. No significant difference and trend was observed in preslaughter weight, empty body weight, carcass weights, dressing percentage, wholesale cuts and edible and non-edible portions of experimental animals. Similarly, no significant variation could be seen in bone and meat yield (in per cent) and their ratios in various wholesale cuts among the dietary treatments. The roughage-to-concentrate ratio did not affect the chemical composition of meat; however, the fat content of meat was linearly increased with increase in the proportion of concentrate in the diets. The results of the experiment indicated that SSB can be included at 60 % level in the complete diet for economical mutton production from growing Nellore × Deccani ram lambs

RNA-Seq gene expression estimation with read mapping uncertainty

Motivation: RNA-Seq is a promising new technology for accurately measuring gene expression levels. Expression estimation with RNA-Seq requires the mapping of relatively short sequencing reads to a reference genome or transcript set. Because reads are generally shorter than transcripts from which they are derived, a single read may map to multiple genes and isoforms, complicating expression analyses. Previous computational methods either discard reads that map to multiple locations or allocate them to genes heuristically

IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection.

Type 2 immune responses are essential in protection against intestinal helminth infections. In this study we show that IL-22, a cytokine important in defence against bacterial infections in the intestinal tract, is also a critical mediator of anti-helminth immunity. After infection with Nippostrongylus brasiliensis, a rodent hookworm, IL-22-deficient mice showed impaired worm expulsion despite normal levels of type 2 cytokine production. The impaired worm expulsion correlated with reduced goblet cell hyperplasia and reduced expression of goblet cell markers. We further confirmed our findings in a second nematode model, the murine whipworm Trichuris muris. T.muris infected IL-22-deficient mice had a similar phenotype to that seen in N.brasiliensis infection, with impaired worm expulsion and reduced goblet cell hyperplasia. Ex vivo and in vitro analysis demonstrated that IL-22 is able to directly induce the expression of several goblet cell markers, including mucins. Taken together, our findings reveal that IL-22 plays an important role in goblet cell activation, and thus, a key role in anti-helminth immunity