The Effect of Female Pheromone on the Functional State of Young Men

Introduction. Modern studies show that the presentation of smell of female mice before male mice infection with the influenza virus activates non-specific immune response which protects male mice from infection of the upper respiratory tract and reducing their mortality. However the researches have not studied the adaptive effect of female sexual pheromone on men.The aim of this work was to study the effect of odor of synthetic analogue of natural female pheromone (Osmopherine) on the functional state of young men often suffering from acute respiratory infections (ARI) and allergic diseases.Materials and methods. We studied four-week effect of Osmopherine on the psycho-emotional state, the concentration of hormones (testosterone, cortisol) and immunoglobulins (sIgA, total IgG), the percentage of leukocytes in the blood and the individual smell of young men, who have ARI more than 4 times a year or an allergic disease. The study involved 117 boys aged 19–23 years. Olfactory testing of individual odor samples of young men was carried out by young women (n = 32) aged 18–22 years.Results. It was found that female pheromone has a positive effect on the functional state of men with different immune systems. However its effect is caused by the pathogenesis of the disease. Application of Osmopherine in men with reduced immunity causes an increased activity, improved mood, reduced anxiety and stabilized testosterone level. Men with allergic diseases did not have the same effect. A significant 2 times decrease in the content of eosinophils was found, which indirectly indicates the decrease in the activity of the delayed phase of allergic reactions of immediate type.Conclusion. The immune system functions according to its internal laws and programs. However its activity for the benefit of the whole body is integrated and regulated by the nervous and endocrine systems. Therefore modulating effect on the functioning of the immune system can be mediated through the presentation of pheromones

Increasing the efficiency of nuclear power plant equipment at the design stage

It is possible to increase the efficiency of the nuclear power plants equipment in various ways. In particular, one of the most relevant is the active use of computer modeling at different stages of work. The effectiveness the software package used directly affects the quality of the installation equipment. Depending on the stage at which the software package is used, it has various priority properties for the most effective application

Russian and foreign steam generators for NPP power units with wet steam turbines

The main aim of the current study is to analyze advantages and shortcomings of horizontal and vertical types of steam generator design. Design solutions and experience of operation of steam generators of horizontal type accepted in Russia and of vertical type applied by Westinghouse, Combustion Engineering, Siemens, Mitsubishi, Doosan were analyzed within the framework of the present study. It was established that steam generator equipment of horizontal type is characterized by disadvantages of design, technological and operational nature. Thus, horizontal steam generators with dimensions permissible for railroad transportation and, for VVER-1200 with reactor vessel diameter equal to 5 m, by water transport as well, have exhausted the possibilities for further significant increase of the per unit electric power. The demonstrated advantages of vertical-type steam generators are as follows: 1) absence of stagnant zones within the second cooling circuit; 2) uniformity of heat absorption efficiency of the heating surface that ensures improved conditions for moisture separation; 3) increased temperature drop with parameters of generated steam elevated by 0.3 – 0.4 MPa. Conclusion was made on the advisability of introduction of steam generators with vertical-type layout in the Russian nuclear power generation

Steam reheater with helical tube bundle for wet steam turbine

Since steam heat exchangers, used at steam cycle of Russian nuclear power stations, were designed while the knowledge about the separation and the heat exchange processes was limited, deviations between its empirical and theoretical characteristics occur. This limitation also determined application of heating pipes with simple straight shape rather than curved. This study explores a steam heat exchanger with helical heating pipes. It was shown that the model may work stably within the range of parameters, simulating work conditions of the moisture separator and steam reheater at Leningrad nuclear power plant. The experiment included processing of pure water steam as well as mixture of steam and nitrogen. It was obtained a relationship between empirical the heat transfer coefficient and the steam mass flow rate. It was noted that presence of incondensable gas does not affect significantly the heat transfer from the coils, processing high pressure steam

The new ways to increase the efficiency of thermal hydraulic processes in systems of separation and reheating of NPP wet steam turbines

Systems of moisture separators - reheaters (SMSR) were created simultaneously with the powerful turbines development of nuclear power plants operating on a wet steam from the early 60s of the 20th century. This lengthy experience made the opportunity to identify the moisture separators - reheaters (MSRs) drawbacks, to determine the objectives for their design and computation improvement in order to increase thermodynamic efficiency, MSR devices and MSR systems reliability in general. Based on maintenance experience and results of our and other researchers’ model and field studies this article discusses potential ways of further development and improvement, taking into consideration both SMSR components (separation and reheating) under conditions of their flow in one apparatus

Comparative efficiency of applying heat pumps within the system of disposal of the condensation heat of heat machines for heating ice arenas

The analysis of the application of two energy-efficient technologies for air heating of ice arenas is considered, such as the use of heat pumps and a system for partial utilization of heat of condensation of heat engines. The actual energy costs during the operation of two systems are studied, such as the consumption of heat and electricity from heat and power supply organizations, as well as the costs of modernization. The weaknesses and positive aspects of the studied systems for air heating of ice arenas are revealed. Comparison of the calculated data obtained as a result of calculations with the actual data obtained in the research institution in which the study is carried out. An example of actual effective modernization of existing engineering systems is shown, as a result of which the energy efficiency of existing systems is increased. Additionally, we have performed the calculation of reducing thermal pollution with introduction of the investigated systems

Delivery of <i>B. subtilis</i> into Animal Intestine Using Chitosan-Derived Bioresorbable Gel Carrier: Preliminary Results

The oral delivery of bacteria in the human intestine is of great interest because of its potential to correct the gut microbiota and treat inflammatory bowel diseases. The aim of this study was to evaluate sodium N-(2-sulfonatoethyl)chitosan gel cross-linked with glutaraldehyde as a delivery carrier for probiotic bacteria to the gut using in vitro and in vivo experiments. The bacterial test strain was B. subtilis 20. The cytotoxicity of the gel was evaluated via cell culture using flow cytometry and light microscopy. The gel as a delivery system was assessed by the dye release in medium with different pH levels in vitro, and by bacterial titer monitoring in mouse feces using the microbiology method in vivo. Results of an in vitro experiment showed that tested gel has no cytotoxicity. The use of gel as a carrier for bacterial delivery into the intestine was more effective than oral gavage of bacterial suspension. Therefore, gel delivery of bacteria decreased the titer level by up to two times. However, a gavage of bacterial suspension decreased the titer level by over 200 times. Tested gel has the potential to be a carrier for the safe delivery of bacteria to the intestine through the stomach, reducing the rate of the elimination of probiotic bacteria from the intestine

Autoclaved Diet with Inactivated Spores of Bacillus spp. Decreased Reproductive Performance of Muc2&minus;/&minus; and Muc2+/&minus; Mice

Within barrier facilities, autoclaved diet and bedding are used for husbandry of laboratory rodents. Bacillus spp. are ubiquitous in nature and some of them are known as probiotics. Inactivation of the Bacillus spores and reduction of the diet nutritional value due to autoclavation could be especially critical for immunodeficient mice. We studied the effect of the autoclaved and non-autoclaved diets on the reproductive performance and the age of prolapse manifestation in Muc2&minus;/&minus; mice with impaired gut barrier function and, therefore, sensitive to change of microbiota. We found that the non-autoclaved diet led to enhancement of the fertility index of Muc2&minus;/&minus; and Muc2+/&minus; female mice. The non-autoclaved diet affected the prolapse of Muc2&minus;/&minus; mice that occurred later in comparison with females eating the autoclaved diet. We showed that Bacillus spp. was present in the non-autoclaved diet and feces of mice on the non-autoclaved diet. Bacterial strains of the non-autoclaved diet and feces belonged to B. amyloliquefaciens, B. thuringiensis, B. subtilis, Lysinibacillus macrolides, B. cereus, and other representatives of Bacillus spp. Moreover, autoclavation of the diet affected on the percent of the blood and spleen immune cells, the bacterial composition of the intestine, and increased the level of methionine in the thigh muscle of mice. Enhanced reproductive performance and delayed prolapse manifestation in Muc2&minus;/&minus; mice could be due to improved digestion, as Bacillus spp. from diet and feces had enzymatic activity

Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus

In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus—the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy