13 research outputs found
Isolation and production of human monoclonal antibody proteins against a <em>Toxocara canis</em> excretory-secretory recombinant antigen
Molecular Aggregation of L-isoleucine in Aqueous Solution and its Impact on the Determination of Solubility and Nucleation Kinetics
Comparison between the solubility of L-Isoleucine as measured using gravimetric and dissolution methods reveals significant differences which is consistent with the presence of solute aggregation in solution. Calculation of the critical aggregation concentration confirms this analysis revealing this concentration to lie between the two measured solubility values suggesting the existence of a stability zone roughly defined by the temperature/concentration range of 40°C/33g/L to 75°C/42g/L in which a mixture of free and aggregated molecules under the slurried conditions appear to exist. Dynamic light scattering result reveals that the aggregate size lies within the range of 40-170 nm. The potential impact of relying on the measurement of solubility using gravimetric data based on solution isolation of the slurried state for such systems is highlighted through a comparison of the values of representative crystallization parameters such as metastable zone width (MSZW) and nucleation kinetics as determined using solubilities derived from both methodologies. Reduction in solution pH in the aggregated molecular state is consistent with the aggregates being formed from neutral species suggesting, in turn, that this compound might crystallize via a 2-step nucleation process. This research suggests that caution should be exercised when using solubility data derived from gravimetric measurements particularly for amphiphilic molecules where solute ordering in solution might be expected
The Lipid Paradox is present in ST-elevation but not in non-ST-elevation myocardial infarction patients:Insights from the Singapore Myocardial Infarction Registry
Lowering low-density lipoprotein (LDL-C) and triglyceride (TG) levels form the cornerstone approach of cardiovascular risk reduction, and a higher high-density lipoprotein (HDL-C) is thought to be protective. However, in acute myocardial infarction (AMI) patients, higher admission LDL-C and TG levels have been shown to be associated with better clinical outcomes - termed the 'lipid paradox'. We studied the relationship between lipid profile obtained within 72 hours of presentation, and all-cause mortality (during hospitalization, at 30-days and 12-months), and rehospitalization for heart failure and non-fatal AMI at 12-months in ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) patients treated by percutaneous coronary intervention (PCI). We included 11543 STEMI and 8470 NSTEMI patients who underwent PCI in the Singapore Myocardial Infarction Registry between 2008-2015. NSTEMI patients were older (60.3 years vs 57.7 years, p < 0.001) and more likely to be female (22.4% vs 15.0%, p < 0.001). In NSTEMI, a lower LDL-C was paradoxically associated with worse outcomes for death during hospitalization, within 30-days and within 12-months (all p < 0.001), but adjustment eliminated this paradox. In contrast, the paradox for LDL-C persisted for all primary outcomes after adjustment in STEMI. For NSTEMI patients, a lower HDL-C was associated with a higher risk of death during hospitalization but in STEMI patients a lower HDL-C was paradoxically associated with a lower risk of death during hospitalization. For this endpoint, the interaction term for HDL-C and type of MI was significant even after adjustment. An elevated TG level was not protective after adjustment. These observations may be due to differing characteristics and underlying pathophysiological mechanisms in NSTEMI and STEMI
Selenium added unripe carica papaya pulp extracts enhance wound repair through TGF-β1 and VEGF-a signalling pathway
Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells
Cenostigma macrophyllum Tul. on the healing of skin wounds in rats with Diabetes mellitus
Modification of cellulose degree of polymerization by superheated steam treatment for versatile properties of cellulose nanofibril film
Hydrogeochemistry and groundwater quality assessment of the multilayered aquifer in Lower Kelantan Basin, Kelantan, Malaysia
Continual expansion of population density, urbanization, agriculture, and industry in most parts of the world has increased the generation of pollution, which contributes to the deterioration of surface water quality. This causes the dependence on groundwater sources for their daily needs to accumulate day by day, which raises concerns about their quality and hydrogeochemistry. This study was carried out to increase understanding of the geological setup and assess the groundwater hydrogeochemical characteristics of the multilayered aquifers in Lower Kelantan Basin. Based on lithological data correlation of exploration wells, the study area can be divided into three main aquifers: shallow, intermediate and deep aquifers. From these three aquifers, 101 groundwater samples were collected and analyzed for various parameters. The results showed that pH values in the shallow, intermediate and deep aquifers were generally acidic to slightly alkaline. The sequences of major cations and anions were Na+ > Ca2+ > Mg2+ > K+ and HCO3− > Cl− > SO42− > CO32−, respectively. In the intermediate aquifer, the influence of ancient seawater was the primary factor that contributed to the elevated values of electrical conductivity (EC), Cl− and total dissolved solids (TDS). The main facies in the shallow aquifer were Ca–HCO3 and Na–HCO3 water types. The water types were dominated by Na–Cl and Na–HCO3 in the intermediate aquifer and by Na–HCO3 in the deep aquifer. The Gibbs diagram reveals that the majority of groundwater samples belonged to the deep aquifer and fell in the rock dominance zone. Shallow aquifer samples mostly fell in the rainfall zone, suggesting that this aquifer is affected by anthropogenic activities. In contrast, the results suggest that the deep aquifer is heavily influenced by natural processes