4,257 research outputs found
Structural Characterization of H1N1 Nucleoprotein-Nucleozin Binding Sites
published_or_final_versio
An artificial intelligence driven multi-feature extraction scheme for big data detection
© 2019 IEEE. The Internet improves the speed of information dissemination, and the scale of unstructured text data is expanding and increasingly being used for mass communication. Although these large amounts of data meet the infinite demand, it is difficult to find public focus in a timely manner. Therefore, information extraction from big data has become an important research issue, and there are many published studies on big data processing at home and abroad. In this paper, we propose a multi-feature keyword extraction method, and based on this, an artificial intelligence driven big data MFE scheme is designed, then an application example of the general scheme is expanded and detailed. Taking news as the carrier, this scheme is applied to the algorithm design of hot event detection. As a result, a multi-feature fusion clustering algorithm is proposed based on user attention with two main stages. In the first stage, a multi-feature fusion model is developed to evaluate keywords, and this model combines the term frequency and part of speech features. We use it to extract keywords for representing news and events. In the second stage, we perform clustering and detect hot events in accordance with the procedure, and during the composition of news clusters, we analyze several variadic parameters in order to explore the optimal effectiveness. Then, experiments on the news corpus are conducted, and the results show that the approach presented herein performs well
Fr-TM-align: a new protein structural alignment method based on fragment alignments and the TM-score
©2008 Pandit and Skolnick; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article is available from: http://www.biomedcentral.com/1471-2105/9/531doi:10.1186/1471-2105-9-531Background: Protein tertiary structure comparisons are employed in various fields of
contemporary structural biology. Most structure comparison methods involve generation of an
initial seed alignment, which is extended and/or refined to provide the best structural superposition
between a pair of protein structures as assessed by a structure comparison metric. One such
metric, the TM-score, was recently introduced to provide a combined structure quality measure
of the coordinate root mean square deviation between a pair of structures and coverage. Using the
TM-score, the TM-align structure alignment algorithm was developed that was often found to have
better accuracy and coverage than the most commonly used structural alignment programs;
however, there were a number of situations when this was not true.
Results: To further improve structure alignment quality, the Fr-TM-align algorithm has been
developed where aligned fragment pairs are used to generate the initial seed alignments that are
then refined using dynamic programming to maximize the TM-score. For the assessment of the
structural alignment quality from Fr-TM-align in comparison to other programs such as CE and TMalign,
we examined various alignment quality assessment scores such as PSI and TM-score. The
assessment showed that the structural alignment quality from Fr-TM-align is better in comparison
to both CE and TM-align. On average, the structural alignments generated using Fr-TM-align have
a higher TM-score (~9%) and coverage (~7%) in comparison to those generated by TM-align. Fr-
TM-align uses an exhaustive procedure to generate initial seed alignments. Hence, the algorithm is
computationally more expensive than TM-align.
Conclusion: Fr-TM-align, a new algorithm that employs fragment alignment and assembly provides
better structural alignments in comparison to TM-align. The source code and executables of Fr-
TM-align are freely downloadable at: http://cssb.biology.gatech.edu/skolnick/files/FrTMalign/
Coherent spinor dynamics in a spin-1 Bose condensate
Collisions in a thermal gas are perceived as random or incoherent as a
consequence of the large numbers of initial and final quantum states accessible
to the system. In a quantum gas, e.g. a Bose-Einstein condensate or a
degenerate Fermi gas, the phase space accessible to low energy collisions is so
restricted that collisions be-come coherent and reversible. Here, we report the
observation of coherent spin-changing collisions in a gas of spin-1 bosons.
Starting with condensates occupying two spin states, a condensate in the third
spin state is coherently and reversibly created by atomic collisions. The
observed dynamics are analogous to Josephson oscillations in weakly connected
superconductors and represent a type of matter-wave four-wave mixing. The
spin-dependent scattering length is determined from these oscillations to be
-1.45(18) Bohr. Finally, we demonstrate coherent control of the evolution of
the system by applying differential phase shifts to the spin states using
magnetic fields.Comment: 19 pages, 3 figure
Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane
Lipopolysaccharide (LPS) is a main component of the outer membrane of Gram-negative bacteria, which is essential for the vitality of most Gram-negative bacteria and plays a critical role for drug resistance. LptD/E complex forms a N-terminal LPS transport slide, a hydrophobic intramembrane hole and the hydrophilic channel of the barrel, for LPS transport, lipid A insertion and core oligosaccharide and O-antigen polysaccharide translocation, respectively. However, there is no direct evidence to confirm that LptD/E transports LPS from the periplasm to the external leaflet of the outer membrane. By replacing LptD residues with an unnatural amino acid p-benzoyl-L-phenyalanine (pBPA) and UV-photo-cross-linking in E.coli, the translocon and LPS intermediates were obtained at the N-terminal domain, the intramembrane hole, the lumenal gate, the lumen of LptD channel, and the extracellular loop 1 and 4, providing the first direct evidence and “snapshots” to reveal LPS translocation steps across the outer membrane
Hydrostatic pressure does not cause detectable changes to survival of human retinal ganglion
Purpose: Elevated intraocular pressure (IOP) is a major risk factor for glaucoma. One consequence of raised IOP is that ocular tissues are subjected to increased hydrostatic pressure (HP). The effect of raised HP on stress pathway signaling and retinal ganglion cell (RGC) survival in the human retina was investigated. Methods: A chamber was designed to expose cells to increased HP (constant and fluctuating). Accurate pressure control (10-100mmHg) was achieved using mass flow controllers. Human organotypic retinal cultures (HORCs) from donor eyes (<24h post mortem) were cultured in serum-free DMEM/HamF12. Increased HP was compared to simulated ischemia (oxygen glucose deprivation, OGD). Cell death and apoptosis were measured by LDH and TUNEL assays, RGC marker expression by qRT-PCR (THY-1) and RGC number by immunohistochemistry (NeuN). Activated p38 and JNK were detected by Western blot. Results: Exposure of HORCs to constant (60mmHg) or fluctuating (10-100mmHg; 1 cycle/min) pressure for 24 or 48h caused no loss of structural integrity, LDH release, decrease in RGC marker expression (THY-1) or loss of RGCs compared with controls. In addition, there was no increase in TUNEL-positive NeuN-labelled cells at either time-point indicating no increase in apoptosis of RGCs. OGD increased apoptosis, reduced RGC marker expression and RGC number and caused elevated LDH release at 24h. p38 and JNK phosphorylation remained unchanged in HORCs exposed to fluctuating pressure (10-100mmHg; 1 cycle/min) for 15, 30, 60 and 90min durations, whereas OGD (3h) increased activation of p38 and JNK, remaining elevated for 90min post-OGD. Conclusions: Directly applied HP had no detectable impact on RGC survival and stress-signalling in HORCs. Simulated ischemia, however, activated stress pathways and caused RGC death. These results show that direct HP does not cause degeneration of RGCs in the ex vivo human retina
Astrocytic Ion Dynamics: Implications for Potassium Buffering and Liquid Flow
We review modeling of astrocyte ion dynamics with a specific focus on the
implications of so-called spatial potassium buffering, where excess potassium
in the extracellular space (ECS) is transported away to prevent pathological
neural spiking. The recently introduced Kirchoff-Nernst-Planck (KNP) scheme for
modeling ion dynamics in astrocytes (and brain tissue in general) is outlined
and used to study such spatial buffering. We next describe how the ion dynamics
of astrocytes may regulate microscopic liquid flow by osmotic effects and how
such microscopic flow can be linked to whole-brain macroscopic flow. We thus
include the key elements in a putative multiscale theory with astrocytes
linking neural activity on a microscopic scale to macroscopic fluid flow.Comment: 27 pages, 7 figure
Spontaneous symmetry breaking in a quenched ferromagnetic spinor Bose condensate
A central goal in condensed matter and modern atomic physics is the
exploration of many-body quantum phases and the universal characteristics of
quantum phase transitions in so far as they differ from those established for
thermal phase transitions. Compared with condensed-matter systems, atomic gases
are more precisely constructed and also provide the unique opportunity to
explore quantum dynamics far from equilibrium. Here we identify a second-order
quantum phase transition in a gaseous spinor Bose-Einstein condensate, a
quantum fluid in which superfluidity and magnetism, both associated with
symmetry breaking, are simultaneously realized. Rb spinor condensates
were rapidly quenched across this transition to a ferromagnetic state and
probed using in-situ magnetization imaging to observe spontaneous symmetry
breaking through the formation of spin textures, ferromagnetic domains and
domain walls. The observation of topological defects produced by this symmetry
breaking, identified as polar-core spin-vortices containing non-zero spin
current but no net mass current, represents the first phase-sensitive in-situ
detection of vortices in a gaseous superfluid.Comment: 6 pages, 4 figure
Marine Collagen Peptides Reduce Endothelial Cell Injury In Diabetic Rats By Inhibiting Apoptosis And The Expression Of Coupling Factor 6 And Microparticles
The present study aimed to elucidate the role of marine collagen peptides (MCPs) in protection of carotid artery vascular endothelial cells (CAVECs) in type 2 diabetes mellitus (T2DM), and the mechanism underlying this process. In an in vivo experiment, diabetic Wistar rats were divided randomly into four groups (n=10/group): Diabetes control, and three diabetes groups administered low, medium and high doses of MCPs (2.25, 4.5 and 9.0 g/kg body weight/day, respectively). Another 10 healthy rats served as the control. In an in vitro experiment, human umbilical‑vein endothelial cells (HUVECs) were incubated in normal and high concentrations of glucose with or without MCPs (3.0, 15.0 and 30.0 mg/ml, respectively) for 24, 48 or 72 h. Blood vessel/endothelial construction, inflammatory exudation and associated molecular biomarkers in CAVECs were detected and analyzed. The results of the present study demonstrated that in rats, MCP treatment for 4 weeks significantly lowered blood glucose and attenuated endothelial thinning and inflammatory exudation in carotid‑artery vascular endothelial cells. In vitro, the high‑glucose intervention significantly increased cell apoptosis in HUVECs, and medium and high doses of MCPs (4.5 and 9.0 g/kg body weight/day, respectively) partially ameliorated this high glucose‑mediated apoptosis and decreased levels of apoptosis biomarkers. In conclusion, a moderate oral MCP dose (≥4.5 g/kg body weight/day) may be a novel therapeutic tool to protect against early cardiovascular complications associated with T2DM by inhibiting apoptosis and reducing the expression of coupling factor 6 and microparticles. Key words: marine collagen peptide, coupling factor 6, microparticles, cell apoptosis, type 2 diabetes mellitus, cardiovascular complication
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