Nitrous oxide and methane in the Atlantic Ocean between 50 degrees North and 52 degrees South: Latitudinal distribution and sea-to-air flux

We discuss nitrous oxide (N2O) and methane (CH4) distributions in 49 vertical profiles covering the upper 300 m of the water column along two 13,500 km transects between 50°N and 52°S during the Atlantic Meridional Transect (AMT) programme (AMT cruises 12 and 13). Vertical N2O profiles were amenable to analysis on the basis of common features coincident with Longhurst provinces. In contrast, CH4 showed no such pattern. The most striking feature of the latitudinal depth distributions was a well-defined “plume” of exceptionally high N2O concentrations coincident with very low levels of CH4, located between 23.5°N and 23.5°S; this feature reflects the upwelling of deep waters containing N2O derived from nitrification, as identified by an analysis of N2O, apparent oxygen utilization (AOU) and NO3-, and presumably depleted in CH4 by bacterial oxidation. Sea-to-air emissions fluxes for a region equivalent to 42% of the Atlantic Ocean surface area were in the range 0.40–0.68 Tg N2O yr-1 and 0.81–1.43 Tg CH4 yr-1. Based on contemporary estimates of the global ocean source strengths of atmospheric N2O and CH4, the Atlantic Ocean could account for 6–15% and 4–13%, respectively, of these source totals. Given that the Atlantic Ocean accounts for around 20% of the global ocean surface, on unit area basis it appears that the Atlantic may be a slightly weaker source of atmospheric N2O than other ocean regions but it could make a somewhat larger contribution to marine-derived atmospheric CH4 than previously thought

Probing Electron Tunneling Pathways: Electrochemical Study of Rat Heart Cytochromecand Its Mutant on Pyridine-Terminated SAMs

The electron-transfer rates between gold electrodes and adsorbed cytochromes are compared for native cytochrome c and its mutant (K13A) using two different immobilization strategies. A recent study by Niki (Niki, K.; Hardy, W. R.; Hill, M. G.; Li, H.; Sprinkle, J. R.; Margoliash, E.; Fujita, K.; Tanimura, R.; Nakamura, N.; Ohno, H.; Richards, J. H.; Gray, H. B. J. Phys. Chem. B 2003, 107, 9947) showed that the electron-transfer rate for a particular mutant cytochrome c (K13A) is orders of magnitude slower than the native form when electrostatically adsorbed on SAM-coated gold electrodes. The current study directly “links” the protein's heme unit to the SAM, thereby “short circuiting” the electron tunneling pathway. These findings demonstrate that the immobilization strategy can modify the electron-transfer rate by changing the tunneling pathway

Modelling oral adrenal cortisol support

A simplified mathematical model of oral hydrocortisone delivery in adrenal insufficiency is described; the model is based on three components (gastric hydrocortisone, free serum cortisol and bound serum cortisol) and is formulated in terms of linear kinetics, taking into account the dynamics of glucocorticoid-protein binding. Motivated by the need to optimise cortisol replacement in the situations of COVID-19 infection, the model is fitted to recently-published data on 50 mg dosing and earlier data on 10 mg dosing. The fitted model is used to predict typical responses to standard dosing regimes, which involve a larger dose in the morning and 1 or 2 smaller doses later in the day, and the same regimes with doses doubled. In all cases there is a circadian-like response, with early morning nadir. The model is also used to consider an alternative dosing strategy based on four equal and equally-spaced doses of 10, 20 or 30 mg per 24 h, resulting in a more even response resembling a response to sustained inflammatory stress.Comment: 8 pages, 2 figures 1 tabl

Development of Additive Construction Technologies for Application to Development of Lunar/Martian Surface Structures Using In-Situ Materials

For long-duration missions on other planetary bodies, the use of in situ materials will become increasingly critical. As human presence on these bodies expands, so must the breadth of the structures required to accommodate them including habitats, laboratories, berms, radiation shielding for natural radiation and surface reactors, garages, solar storm shelters, greenhouses, etc. Planetary surface structure manufacturing and assembly technologies that incorporate in situ resources provide options for autonomous, affordable, pre-positioned environments with radiation shielding features and protection from micrometeorites, exhaust plume debris, and other hazards. The ability to use in-situ materials to construct these structures will provide a benefit in the reduction of up-mass that would otherwise make long-term Moon or Mars structures cost prohibitive. The ability to fabricate structures in situ brings with it the ability to repair these structures, which allows for the self-sufficiency and sustainability necessary for long-duration habitation. Previously, under the auspices of the MSFC In-Situ Fabrication and Repair (ISFR) project and more recently, under the jointly-managed MSFC/KSC Additive Construction with Mobile Emplacement (ACME) project, the MSFC Surface Structures Group has been developing materials and construction technologies to support future planetary habitats with in-situ resources. One such additive construction technology is known as Contour Crafting. This paper presents the results to date of these efforts, including development of novel nozzle concepts for advanced layer deposition using this process. Conceived initially for rapid development of cementitious structures on Earth, it also lends itself exceptionally well to the automated fabrication of planetary surface structures using minimally processed regolith as aggregate, and binders developed from in situ materials as well. This process has been used successfully in the fabrication of construction elements using lunar regolith simulant and Mars regolith simulant, both with various binder materials. Future planned activities will be discussed as well

Assessing Patients’ Needs in Greek Intensive Care Units. Validation of a Respiratory Patient Satisfaction Scale

Respiratory patients constitute a specific and large category of patients hospitalized in Greek Intensive Care Units (ICUs). These patients have specific needs which differ significantly from other groups of patients treated in general ICUs. Assessing the needs and satisfaction levels of respiratory patients is a crucial issue, related to the quality of care provided in ICU.  Many questionnaires have been developed to measure patient satisfaction or patient needs. However, no previous attempt has been made to develop an instrument focused on respiratory patient needs with the appropriate psychometric properties. The aim of this study was to describe the development and evaluation of the psychometric properties of the Respiratory Patient Satisfaction Scale, and its validity and reliability, by ICU respiratory patients’ satisfaction.Τhe questionnaire was pilot tested with 20 ICU respiratory patients to identify problems of wording or length of the instrument. Based on feedback, the wording was adjusted and certain items were combined. A new convenience sample of 164 ICU respiratory patients then performed a test-retest of the questionnaire. Internal consistency was assessed using Cronbach alpha coefficients and stability of items was evaluated through test and retest comparison and expressed through intraclass correlation coefficient (ICC). The internal consistency reliability coefficients exceed the minimum 0.50 for Cronbach’s coefficient alpha. An exploratory factor analysis revealed seventeen factors, explaining 74.5% of the variability. Respiratory Patient Satisfaction Scale found to be a comprehensive instrument with satisfactory psychometric properties. The results from this study would serve as references for continuous improvement in intensive care practice helping all patients hospitalized in Greek ICUs and not only the respiratory patients. Keywords: Respiratory patients, Intensive Care Unit, Patient Satisfaction Scale, Validation,   Quality care. DOI: 10.7176/JEP/12-17-01 Publication date:June 30th 202

Exploring simulation design for mental health practice preparation: a pilot study with learners and preceptors

The purpose of this exploratory pilot study was to determine the feasibility of delivering mental health practice simulations for occupational therapy learners, and whether different debriefing approaches yielded performance differences over successive simulations. Five clinical preceptors and nine first year MScOT students participated in this mixed-method study. In week one simulations, one student group received preceptor facilitated debriefing while the other group used self-debriefing. Both groups used the same scripted questions informed by an advocacy-inquiry approach. In the second week, both groups received the preceptor-led debriefing. Preceptors rated student performances while students self-rated their confidence, competence screen, and satisfaction using standardized tools. Ratings of simulation performance revealed gaps in practice knowledge and the process of practice. Preceptor-led debriefing for both groups resulted in greater depth of reflection and insight into learning gaps and opportunities for continued improvement. The self-debriefing group reported feeling less confident in the simulations. Preceptors reported the scripted advocacy inquiry debriefing approach helped draw out clinical reasoning that could not be observed from performance alone. Simulation may be an effective teaching tool for developing core practice competencies. Design and debriefing styles appear to impact preceptor feedback and the depth of learner critical reflection. Further study is required for generalization

Researching possible futures to guide leaders towards more effective tertiary education

This research aimed to inform institutional leaders by producing and disseminating a system wide view of what tertiary education might look like in Aotearoa New Zealand, five years into the future. The researchers were responding to a challenge in a speech at the DEANZ 2010 conference by a highly respected national leader (Dr Peter Coolbear). The outcome became known as the DEANZ2016 scenario set. Using JISC scenario planning methodologies, including interviews of 16 national and international education leaders, the scenario set was developed on an X axis depicting the tension between facing the academy vs facing New Zealand employers, professions and iwi, and a Y axis depicting the tension between standardized education vs customization to personalise learning. Each of the four quadrants aimed to expose and contrast potential future scenarios. These were entitled: Articulation, The 'Supermarket', Quality Branded Consortia, and Self Determination. Innovatively disseminated via the web, the DEANZ2016 website became the most popular section of the larger Ako Aotearoa website, which stimulated further research into uptake and impact of the project

Researching possible futures to guide leaders towards more effective tertiary education

This research aimed to inform institutional leaders by producing and disseminating a system wide view of what tertiary education might look like in Aotearoa New Zealand, five years into the future. The researchers were responding to a challenge in a speech at the DEANZ 2010 conference by a highly respected national leader (Dr Peter Coolbear). The outcome became known as the DEANZ2016 scenario set. Using JISC scenario planning methodologies, including interviews of 16 national and international education leaders, the scenario set was developed on an X axis depicting the tension between facing the academy vs facing New Zealand employers, professions and iwi, and a Y axis depicting the tension between standardized education vs customization to personalise learning. Each of the four quadrants aimed to expose and contrast potential future scenarios. These were entitled: Articulation, The 'Supermarket', Quality Branded Consortia, and Self Determination. Innovatively disseminated via the web, the DEANZ2016 website became the most popular section of the larger Ako Aotearoa website, which stimulated further research into uptake and impact of the project