7 research outputs found
The Influence of General Anesthesia on the Brain in Aged Patients with Previous Ischemic Cerebrovascular Disease
Postnatal development of the rat corticospinal tract, with special reference to confocal laser scanning microscopy of growing axons
Glutamate Transporter GLT-1 Is Transiently Localized on Growing Axons of the Mouse Spinal Cord before Establishing Astrocytic Expression
The glutamate transporter GLT-1 is expressed in astrocytes of the mature brain and spinal cord. In the present study, we examined its expression in the developing mouse spinal cord. By in situ hybridization, 35 S-labeled antisense oligonucleotide probes for GLT-1 mRNA consistently labeled the mantle zone/ gray matter from embryonic day 11 through the adult stage. However, immunohistochemistry with a specific antibody visualized distinct regional and cellular localizations during the time between the fetal and postnatal stages. At fetal stages, GLT-1 immunoreactivity predominated in the marginal zone/white matter, observed as tiny puncta in cross-sections and as thin fibers in longitudinal sections. The GLT-1-immunopositive structures were also labeled for neuron-specific enolase, a glycolytic enzyme specific to postmitotic neurons and endocrine cells. By electron microscopy, GLT-1 immunoreactivit