STUDIES ON PARABIOTIC UNION

In the study on the homotransplantation of the skin, parabiotic union is frequently used. In the present work, parabiosis was carried out between the uniform strain of Wistar rats and uniform strain of Sprague-Dawley rats, and the cytotoxic antibodies were examined. First, the skin of the Sprague Dawley rat was transplanted to the Wistar rats and the antibodies were measured by Terasaki’s improved microdroplet assay method for cytotoxin before the transplantation and also on the 3rd, 5th, 7th, and 10th day after the second transplantation. The cytotoxic antibodies were not found before the transplantation but were observed with the course of time and also after the second transplantation. The mechanism of the rejection of the transplant is still not clear, but the appearance of cytotoxic antibodies has been confirmed and is now regarded as important. Therefore, an anti-rat-lymphocyte rabbit serum was prepared and an attempt was made to prolong the graft viability in parabiosis

PULMONARY EMBOLISM EXPERIMENTAL STUDY ON EMBOLECTOMY

Pulmonary embolism has been thought as a relatively rare disease in Japan. In the recent reports, however, the rate of increase of this disease is almost similar to that in the United States. We reported already about the experimental studies on pulmonary embolism by our original method and studied the roentgenographic changes in relation to the pathohistological findings. In this paper we will report on the experimental studies on embolectomy in the pulmonary artery, especially on the limitation of the effectiveness of this procedure. A total of 45 dogs were used for this study. The embolectomy and thrombectomy by thoracotomy were performed after 1, 2, 3 and 4 days and 1 week following the insertion of the Laminaria-embolus. During thoracotomy the pressure of the pulmonary artery was measured before and after embolectomy. X-rays of the chest were taken after 1 and 3 days and 1 week following embolectomy and we made pathohistological examinations of the resected lungs. We were not able to find any literature on the limitation of the effectiveness of embolectomy in the pulmonary artery. We suppose from these findings that embolectomy should be done 1 or 2 days after the pulmonary embolism occurs and in the early stage when the changes caused by pulmonary embolism are still reversible