49 research outputs found
ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΡΠΈΠ»ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠ³ΠΎ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Π° ph20 ΠΈ Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Π° ΡΠΈΠ½Π΅Π³Π½ΠΎΠΉΠ½ΠΎΠΉ ΠΏΠ°Π»ΠΎΡΠΊΠΈ ph57 ΠΏΡΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΡ ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΠΈ Π² ΠΎΡΡΠΎΠΏΠ΅Π΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΈΠ· ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΠ° (ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅Π½ΡΠ°)
Background: The problem of bacterial colonization of implants used in medical practice continues to be relevant regardless of the material of the implant. Particular attention deserves polymeric implants, which are prepared ex tempore from polymethyl methacrylate, for example - duting orthopedic surgical interventions (so-called "bone cement"). The protection of such implants by antibiotic impregnation is subjected to multiple criticisms, therefore, as an alternative to antibiotics, lytic bacteriophages with a number of unique advantages can be used - however, no experimental studies have been published on the possibility of impregnating bacteriophages into polymethyl methacrylate and their antibacterial activity assessment under such conditions.Aims: to evaluate the possibility of physical placement of bacteriophages in polymethylmethacrylate and to characterize the lytic antibacterial effect of two different strains of bacteriophages when impregnated into polymer carrier ex tempore during the polymerization process in in vitro model.Materials and methods: Β First stage - Atomic force microscopy (AFM) of polymethyl methacrylate samples for medical purposes was used to determine the presence and size of caverns in polymethyl methacrylate after completion of its polymerization at various reaction Β temperatures (+6β¦+25Β°C and +18β¦+50Β°C).The second stage was performed in vitro and included an impregnation of two different bacteriophage strains (phage ph20 active against S. aureus and ph57 active against Ps. aeruginosa) into polymethyl methacrylate during the polymerization process, followed by determination of their antibacterial activity.Results: ACM showed the possibility of bacteriophages placement in the cavities of polymethyl methacrylate - the median of the section and the depth of cavities on the outer surface of the polymer sample polymerized at +18β¦+50Β°C were 100.0 and 40.0 nm, respectively, and on the surface of the transverse cleavage of the sample - 120.0 and 100.0 nm, respectively, which statistically did not differ from the geometric dimensions of the caverns of the sample polymerized at a temperature of +6β¦+25Β°C.The study of antibacterial activity showed that the ph20 bacteriophage impregnated in polymethyl methacrylate at +6β¦+25Β°C lost its effective titer within the first six days after the start of the experiment, while the phage ph57 retained an effective titer for at least 13 days.Conclusion: the study confirmed the possibility of bacteriophages impregnation into medical grade polymethyl methacrylate, maintaining the effective titer of the bacteriophage during phage emission into the external environment, which opens the way for the possible application of this method of bacteriophage delivery in clinical practice. It is also assumed that certain bacteriophages are susceptible to aggressive influences from the chemical components of "bone cement" and / or polymerization reaction products, which requires strict selection of bacteriophage strains that could be suitable for this method of delivery.ΠΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅. ΠΡΠΎΠ±Π»Π΅ΠΌΠ° Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΡ
Β Π²Β ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² ΠΈΠ· ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ² ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ°Π΅Ρ ΠΎΡΡΠ°Π²Π°ΡΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΉ, Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π΄Π»Ρ ΠΈΡ
ΠΈΠ·Π³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΡΠ΄Π΅Π»ΡΠ½ΠΎΠ³ΠΎ Π²Π½ΠΈΠΌΠ°Π½ΠΈΡ Π·Π°ΡΠ»ΡΠΆΠΈΠ²Π°ΡΡ ΠΈΠΌΠΏΠ»Π°Π½ΡΠΈΡΡΠ΅ΠΌΡΠ΅Β Π²Β ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠ΅ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΈΠ·Π³ΠΎΡΠΎΠ²Π»ΡΡΡ exΒ tempore (ΠΏΠΎ ΠΌΠ΅ΡΠ΅ Π½Π°Π΄ΠΎΠ±Π½ΠΎΡΡΠΈ) ΠΈΠ· ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΠ°, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ ΠΏΡΠΈ ΠΎΡΡΠΎΠΏΠ΅Π΄ΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°Ρ
(ΡΠ°ΠΊ Π½Π°Π·ΡΠ²Π°Π΅ΠΌΡΠΉ ΠΊΠΎΡΡΠ½ΡΠΉ ΡΠ΅ΠΌΠ΅Π½Ρ). ΠΠ°ΡΠΈΡΠ° ΡΠ°ΠΊΠΈΡ
ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΎΠ² ΠΏΡΡΠ΅ΠΌ ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΠΈΒ Π²Β Π½ΠΈΡ
Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ² ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π΅ΡΡΡ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΊΡΠΈΡΠΈΠΊΠ΅, ΠΏΠΎΡΡΠΎΠΌΡΒ Π²Β ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Ρ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ°ΠΌ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΈ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ ΡΡΠ΄ΠΎΠΌ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ², ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΡΠ°Π±ΠΎΡ ΠΏΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²Β Π²Β ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΒ ΠΈΒ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈΒ Π²Β ΡΠ°ΠΊΠΈΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Β Π²Β Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ Π½Π΅ ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½ΠΎ.Β Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΒ β ΠΈΠ·ΡΡΠΈΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ΅Π½ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²Β Π²Β ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΠ΅Β ΠΈ Π²Β ΠΌΠΎΠ΄Π΅Π»ΠΈΒ inΒ vitroΒ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°ΡΡ Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠΉ ΡΡΡΠ΅ΠΊΡ Π΄Π²ΡΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ² ΠΏΡΠΈ ΠΈΡ
ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΠΈΒ Π²Β ΠΈΠ·Π³ΠΎΡΠ°Π²Π»ΠΈΠ²Π°Π΅ΠΌΡΠΉ exΒ tempore ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ½ΡΠΉ Π½ΠΎΡΠΈΡΠ΅Π»Ρ Π½Π° ΡΡΠ°ΠΏΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈ.Β ΠΠ΅ΡΠΎΠ΄Ρ. ΠΠ΅ΡΠ²ΡΠΌ ΡΡΠ°ΠΏΠΎΠΌ Π±ΡΠ»Π° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π°Β Π°ΡΠΎΠΌΠ½ΠΎ-ΡΠΈΠ»ΠΎΠ²Π°Ρ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ (ΠΠ‘Π) ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΠ° ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΈΡ Π΄Π»Ρ Π²ΡΡΡΠ½Π΅Π½ΠΈΡ Π½Π°Π»ΠΈΡΠΈΡΒ ΠΈΒ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠ² ΠΊΠ°Π²Π΅ΡΠ½, ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π²ΡΠΈΡ
ΡΡ ΠΏΠΎΡΠ»Π΅ Π·Π°Π²Π΅ΡΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΌ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΌΠ΅ΡΠΈ (+6β¦+25 Β°CΒ ΠΈΒ +18β¦+50 Β°C). ΠΡΠΎΡΡΠΌ ΡΡΠ°ΠΏΠΎΠΌ inΒ vitro Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΡ Π΄Π²ΡΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ² (ph20, Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎΒ Π²Β ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ StaphylococcusΒ aureus,Β ΠΈΒ ph57, Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎΒ Π²Β ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ PseudomonasΒ aeruginosa)Β Π²Β ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°Ρ Π½Π° ΡΡΠ°ΠΏΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈΒ ΡΒ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈΡ
Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ.Β Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ.Β ΠΒ Ρ
ΠΎΠ΄Π΅ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ ΠΠ‘Π ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠ°Π·ΠΌΠ΅ΡΠ΅Π½ΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²Β Π²Β ΠΊΠ°Π²Π΅ΡΠ½Π°Ρ
ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°ΡΠ°: ΠΌΠ΅Π΄ΠΈΠ°Π½Π° ΡΠ΅ΡΠ΅Π½ΠΈΡΒ ΠΈΒ Π³Π»ΡΠ±ΠΈΠ½Ρ ΠΊΠ°Π²Π΅ΡΠ½ Π½Π° Π²Π½Π΅ΡΠ½Π΅ΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠ°, ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ +18β¦+50 Β°C, ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 100,0Β ΠΈΒ 40,0Β Π½ΠΌ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ,Β Π°Β Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΏΠΎΠΏΠ΅ΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠΎΠ»Π° ΠΎΠ±ΡΠ°Π·ΡΠ°Β β 120,0Β ΠΈΒ 100,0Β Π½ΠΌ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ, ΡΡΠΎ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ ΠΎΡΠ»ΠΈΡΠ°Π»ΠΎΡΡ ΠΎΡ Π³Π΅ΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°Π·ΠΌΠ΅ΡΠΎΠ² ΠΊΠ°Π²Π΅ΡΠ½ ΠΎΠ±ΡΠ°Π·ΡΠ°, ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ +6β¦+25 Β°C. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, ΡΡΠΎ ΠΈΠΌΠΏΡΠ΅Π³Π½ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ ΠΏΡΠΈ +6β¦+25 Β°CΒ Π²Β ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°Ρ ΡΡΠ°ΡΠΈΠ»ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΠΉ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Β ph20 ΡΡΡΠ°ΡΠΈΠ» ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΡΠΈΡΡ ΡΠΆΠ΅Β Π²Β ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΡΡ
ΡΠ΅ΡΡΠΈ ΡΡΡΠΎΠΊΒ ΡΒ ΠΌΠΎΠΌΠ΅Π½ΡΠ° Π½Π°ΡΠ°Π»Π° ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ ΡΠΈΠ½Π΅Π³Π½ΠΎΠΉΠ½ΡΠΉ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Β ph57 ΡΠΎΡ
ΡΠ°Π½ΡΠ» ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΡΠΈΡΡ ΠΊΠ°ΠΊ ΠΌΠΈΠ½ΠΈΠΌΡΠΌΒ Π²Β ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 13Β ΡΡΡ.Β ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅.Β ΠΒ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ Π±ΡΠ»Π° ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΈΠΌΠΏΡΠ΅Π³Π½Π°ΡΠΈΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²Β Π²Β ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ»ΠΌΠ΅ΡΠ°ΠΊΡΠΈΠ»Π°Ρ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ Π½Π°Π·Π½Π°ΡΠ΅Π½ΠΈΡΒ ΡΒ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠΈΡΡΠ° Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³Π° ΠΏΡΠΈ Π΅Π³ΠΎ ΡΠΌΠΈΡΡΠΈΠΈ Π²ΠΎ Π²Π½Π΅ΡΠ½ΡΡ ΡΡΠ΅Π΄Ρ, ΡΡΠΎ ΠΎΡΠΊΡΡΠ²Π°Π΅Ρ ΠΏΡΡΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠ°ΠΊΠΎΠ³ΠΎ ΡΠΏΠΎΡΠΎΠ±Π° Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²Β Π²Β ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅. Π’Π°ΠΊΠΆΠ΅ ΡΠ΄Π΅Π»Π°Π½Ρ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΡΒ ΠΎΒ Π²Π΅ΡΠΎΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ΄Π²Π΅ΡΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ² Π°Π³ΡΠ΅ΡΡΠΈΠ²Π½ΡΠΌ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡΠΌ ΡΠΎ ΡΡΠΎΡΠΎΠ½Ρ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Β«ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠ΅Π½ΡΠ°Β» ΠΈ/ΠΈΠ»ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΡΠ΅Π°ΠΊΡΠΈΠΈ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠΈΠ·Π°ΡΠΈΠΈ, ΡΡΠΎ ΡΡΠ΅Π±ΡΠ΅Ρ ΡΡΡΠΎΠ³ΠΎΠ³ΠΎ ΠΎΡΠ±ΠΎΡΠ° ΠΏΡΠΈΠ³ΠΎΠ΄Π½ΡΡ
Π΄Π»Ρ ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠ³ΠΎ ΡΠΏΠΎΡΠΎΠ±Π° Π΄ΠΎΡΡΠ°Π²ΠΊΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠ°Π³ΠΎΠ²
Invasive aspergillosis in patients with COVID-19 in intensive care units: results of a multicenter study
Objective.
To study risk factors, clinical and radiological features and effectiveness of the treatment of invasive aspergillosis (IA) in adult patients with COVID-19 (COVID-IA) in intensive care units (ICU).
Materials and Methods.
A total of 60 patients with COVID-IA treated in ICU (median age 62 years, male β 58%) were included in this multicenter prospective study. The comparison group included 34 patients with COVID-IA outside the ICU (median age 62 years, male β 68%). ECMM/ISHAM 2020 criteria were used for diagnosis of CAPA, and EORTC/MSGERC 2020 criteria were used for evaluation of the treatment efficacy. A case-control study (one patient of the main group per two patients of the control group) was conducted to study risk factors for the development and features of CAPA. The control group included 120 adult COVID-19 patients without IA in the ICU, similar in demographic characteristics and background conditions. The median age of patients in the control group was 63 years, male β 67%.
Results.
64% of patients with COVID-IA stayed in the ICU. Risk factors for the COVID-IA development in the ICU: chronic obstructive pulmonary disease (OR = 3.538 [1.104β11.337], p = 0.02), and prolonged (> 10 days) lymphopenia (OR = 8.770 [4.177β18.415], p = 0.00001). The main location of COVID-IA in the ICU was lungs (98%). Typical clinical signs were fever (97%), cough (92%), severe respiratory failure (72%), ARDS (64%) and haemoptysis (23%). Typical CT features were areas of consolidation (97%), hydrothorax (63%), and foci of destruction (53%). The effective methods of laboratory diagnosis of COVID-IA were test for galactomannan in BAL (62%), culture (33%) and microscopy (22%) of BAL. The main causative agents of COVID-IA are A. fumigatus (61%), A. niger (26%) and A. flavus (4%). The overall 12-week survival rate of patients with COVID-IA in the ICU was 42%, negative predictive factors were severe respiratory failure (27.5% vs 81%, p = 0.003), ARDS (14% vs 69%, p = 0.001), mechanical ventilation (25% vs 60%, p = 0.01), and foci of destruction in the lung tissue on CT scan (23% vs 59%, p = 0.01).
Conclusions.
IA affects predominantly ICU patients with COVID-19 who have concomitant medical conditions, such as diabetes mellitus, hematological malignancies, cancer, and COPD. Risk factors for COVID-IA in ICU patients are prolonged lymphopenia and COPD. The majority of patients with COVID-IA have their lungs affected, but clinical signs of IA are non-specific (fever, cough, progressive respiratory failure). The overall 12-week survival in ICU patients with COVID-IA is low. Prognostic factors of poor outcome in adult ICU patients are severe respiratory failure, ARDS, mechanical ventilation as well as CT signs of lung tissue destruction
Change in the soilsβ fertility level of tea agrocenoses in the transition to cultivation without mineral fertilizers in the humid-subtropical zone of Russia
Research was carried out on the basis of preserved field multifactor experiment on tea crop (Camellia sinensis (L.) Kuntze) in the conditions of the Sochi Black Sea coast. The application of NPK fertilizers in different doses and combinations according to the experimental scheme was carried out annually from 1986 to 2011. Since 2012, the use of fertilizers has been completely discontinued. The fertility indicators of long-fertilized brown forest acidic soils (in layers 0-20/20-40 cm) were compared with those after 7-8 years of fertilizer withdrawal. During the period of fertilizersβ application, the level of soilsβ nitrogen supply significantly exceeded the control (by 30-75/30-56 mg/kg depending on the doses of nitrogen fertilizers). After the fertilizersβ discontinuity, nitrogen supply level equalization occurred in all experimental options. The content of labile phosphorus in soils previously fertilized with high phosphorus doses (120 kg P2O5 ha-1 year-1 and more), during the period of experimentβs conservation decreased by 450-500/350-450 mg/kg, but exceeded the control in 2-2.5 times; in soils previously fertilized by low doses, the indicators have not changed significantly. In soils highly supplied with labile potassium, the content of the element decreased by 70-140 mg/kg. The yield of tea in 2019-2020 was equally low (12-26 cwt/ha) on all options, 2.2-3 times lower than fertilized plantations. After the fertilizersβ withdrawal, there was a decrease in acidity (an average increase in pH by 0.18-0.24/0.12-0.20 units) of agrogenic-acidized soils and an increase in their oppressed respiratory activity (on average by 1.6 times). These changes reflect the tendency of soil self-restoration after the removal of the fertilizersβ load
Imperative and dispositive norms in legal regulation of genetic research in Russia
Β© 2020, Universidad del Zulia. All rights reserved. The article is devoted to the analysis of diverse utilization varieties of regulatory framework at adjusting genetic techniques and global legal practice research. Internal determinants of the development of national systems and external universal laws of legal transformation influence the evolution of views of the professional community of lawyers in Russia and in foreign countries, regarding the choice of dispositive or mandatory ways of regulating genetic research. The discussion on a number of legal institutions and on the choice of effective methods and forms of legal regulation in the world and Russian legal practice continues
Quality of life - the factor of Russia success at present and in the future
The quality of life and human development - these concepts are substantial characteristics of contemporary approaches to the challenges of economic growth and development of society. The quality of life in contemporary quality concepts means the integrated characteristics of social-economic, political, cultural-ideologic, ecologic factors and living conditions and social status of a person. State policy in the field of regulating populationβs quality of life and living standards is the basis of creating totally new work force, essential needs in material wealth, life quality and working conditions indicators. We consider the definition "quality of life" within the concept of human development to define the system of the factors influencing it. We review the modern approaches to the analysis of the quality of life. Features and aspects of quality of life are considered, its role in social and economic development of the country is analysed. The main criteria define specifics of human development and social wellbeing of the population. To evaluate the quality of life of the population we use the statistical analysis of the United Nations (UN), Human Development Index (HDI).We conclude that the level of life is an integrated social and economic category that presents the level of development of physical, immaterial and social needs which influence the quality of life and population wellbeing Index
ΠΠΠ‘Π’ΠΠΠΠ-ΠΠΠΠΠΠΠΠ’ΠΠΠΠ«Π ΠΠΠ ΠΠ Π ΠΠΠΠΠ’ΠΠ― ΠΠΠΠΠΠΠ (ΠΠΠΠ Π) Π£ ΠΠΠΠΠ Π ΠΠΠΠΠ ΠΠΠΠΠΠΠΠΠ - ΠΠΠΠΠΠΠΠΠ ΠΠΠ‘Π’Π ΠΠΠ£Π’Π ΠΠ£Π’Π ΠΠΠΠΠΠ Π ΠΠΠΠΠ’ΠΠ― Π ΠΠ‘ΠΠΠΠΠΠΠ‘Π’ΠΠΠΠ‘Π’ΠΠΠ’ΠΠΠ¬ΠΠΠ ΠΠΠΠΠΠΠ‘Π’ΠΠΠ
Digital Economy and Digital Twins. Main Research Area
The work is devoted to the consideration and research of methods for transforming economic structures that underlie the transition to the dominant digital task of economic processes and their relationship with digital forms of representing economic relations. The main stages of the possible assignment of enterprises and the levels of building digital twins have been determined. Using the example of an object that belongs to the external area of ββthe enterprise β the supplier of the constituent components of the product β it is shown how the transition to the digital form of this object can be built.ΠΠ΅ΡΠ° ΡΠΎΠ±ΠΎΡΠΈ β Π·Π°ΠΏΡΠΎΠΏΠΎΠ½ΡΠ²Π°ΡΠΈ ΠΎΡΠ½ΠΎΠ²Π½Ρ Π΅ΡΠ°ΠΏΠΈ, ΡΠΊΡ Π²ΠΈΠ·Π½Π°ΡΠ°ΡΡ ΠΏΠ΅ΡΠ΅Ρ
ΡΠ΄ Π²ΡΠ΄ Π·Π²ΠΈΡΠ°ΠΉΠ½ΠΈΡ
ΠΏΡΠ΄ΠΏΡΠΈΡΠΌΡΡΠ² Π΄ΠΎ ΠΉΠΎΠ³ΠΎ ΡΠΈΡΡΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠ΄Π°Π½Π½Ρ (ΡΠΈΡΡΠΎΠ²ΠΎΠ³ΠΎ Π΄Π²ΡΠΉΠ½ΠΈΠΊΠ°) Π² ΡΠΌΠΎΠ²Π°Ρ
ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Ρ Π΄ΠΎ ΡΠΈΡΡΠΎΠ²ΠΎΡ Π΅ΠΊΠΎΠ½ΠΎΠΌΡΠΊΠΈ.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠΈΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΡΠ°ΠΏΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ ΠΎΡ ΠΎΠ±ΡΡΠ½ΡΡ
ΠΏΡΠ΅Π΄ΠΏΡΠΈΡΡΠΈΠΉ ΠΊ ΠΈΡ
ΡΠΈΡΡΠΎΠ²ΠΎΠΌΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΡ (ΡΠΈΡΡΠΎΠ²ΠΎΠΌΡ Π΄Π²ΠΎΠΉΠ½ΠΈΠΊΡ) Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄Π° ΠΊ ΡΠΈΡΡΠΎΠ²ΠΎΠΉ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΠΊΠ΅
DYNAMICS OF PLASMA PHENOMENA IN "PLASMA FOCUS" UNDER THE ACTION OF POWERFUL LASER RADIATION
No abstract availabl
ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΠ»ΠΈΠ°ΡΠ½ΠΎΠ³ΠΎ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠ° Π² ΡΠ»Π΅Π·Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
The concentration of ciliary neurotrophic factor (CNTF) was measured in lacrimal fluid (LF) using Human CNTF Quantikine ELISA kit (βR&D Systemsβ, USA) on a ChemWell 2910 automatic analyzer (βAwareness Technology Inc.β, USA). We initially attempted to use commercial kits, designed for serum and plasma CNTF detection, to quantify lacrimal CNTF. The results, however, were rarely above the minimum detection level of the kits, most likely due to matrix complexity and low concentrations of CNTF in diluted LF (LF had to be diluted because of the small volume of collected samples). The optimal sensitivity and the lowest background for the best minimum quantifiable value were determined empirically. Phosphate buffer solution containing 1% bovine serum albumin was selected as an optimal diluent for CNTF measurements in small fluid samples. A standard curve was produced using the calibrating solutions 0-250 pg/ml. Acid treatment of LF samples before the analysis allowed to increase the detectable concentration of the CNTF two-fold. The 1:3 dilution was selected based on the available volume of collected LF and a reasonable variation coefficient. The described protocol allowed to develop a sandwich ELISA optimized for lacrimal CNTF.ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΈΠ»ΠΈΠ°ΡΠ½ΠΎΠ³ΠΎ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠ° β ciliary neurotrophic factor (CNTF) Π² ΡΠ»ΡΠ·Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠΈ (Π‘Π) ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΡΡΠΈΠ½Π½ΡΠΌΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π·Π°ΡΡΡΠ΄Π½Π΅Π½ΠΎ ΠΈΠ·-Π·Π° Π΅Π³ΠΎ Π½ΠΈΠ·ΠΊΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π² ΡΡΡΡΠΊΡΡΡΠ°Ρ
Π³Π»Π°Π·Π° ΠΈ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΡΠ»ΠΈ ΠΏΠΎΠ΄ΠΎΠ±ΡΠ°Π½Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ CNTF Π² Π‘Π ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ΠΠ€Π) Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π½Π°Π±ΠΎΡΠ° Human CNTF Quantikine ELISA Kit (βR&D Systemsβ, Π‘Π¨Π) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ° ChemWell 2910 Combi (βAwareness Technology Inc.β, Π‘Π¨Π). ΠΡΠ΅Π΄Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ½Π°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΏΡΠΎΠ± Π‘Π ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° Π²Π΄Π²ΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ CNTF. ΠΠΎΠ»Π½ΠΎΡΡΡΡ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π°Π½Π°Π»ΠΈΠ·Π° ΠΈ Π²ΡΠ±ΠΎΡ ΡΠ°Π·Π±Π°Π²ΠΈΡΠ΅Π»Ρ Ρ Π½ΠΈΠ·ΠΊΠΎΠΉ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡΡ Π±Π»Π°Π½ΠΊΠ° (0.011) ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ»ΠΈ Π»ΠΈΠ½Π΅ΠΉΠ½ΡΡ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ ΠΊΠ°Π»ΠΈΠ±ΡΠΎΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ Π³ΡΠ°ΡΠΈΠΊΠ° ΠΎΡ 0 Π΄ΠΎ 250 ΠΏΠ³/ΠΌΠ» Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡΡ Π΅Π³ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄Π»Ρ ΡΠ°ΡΡΠ΅ΡΠ° ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ CNTF Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π½ΠΈΠ·ΠΊΠΈΡ
Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ. Π Π°Π±ΠΎΡΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌΡ ΠΠ€Π (Π»ΠΈΠ½Π΅ΠΉΠ½ΠΎΡΡΡ ΠΌΠ΅ΡΠΎΠ΄Π°, ΠΏΡΠ΅Π΄Π΅Π» ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ, Π²ΠΎΡΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ, ΠΈΠ½ΡΠ΅ΡΡΠ΅ΡΠ΅Π½ΡΠΈΡ ΠΌΠ°ΡΡΠΈΡΡ) Π² ΡΠΊΠ°Π·Π°Π½Π½ΠΎΠΌ Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ ΠΊΡΠΈΡΠ΅ΡΠΈΡΠΌ ΠΏΡΠΈΠ΅ΠΌΠ»Π΅ΠΌΠΎΡΡΠΈ Π΄Π»Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ. Π Π°Π·Π²Π΅Π΄Π΅Π½ΠΈΠ΅ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π‘Π Π² ΡΠ΅ΡΡΡΠ΅ ΡΠ°Π·Π°, ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½Π½ΠΎΠ΅ Ρ ΠΊΠΈΡΠ»ΠΎΡΠ½ΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΎΠΉ ΠΏΡΠΎΠ±, ΠΎΠΊΠ°Π·Π°Π»ΠΎΡΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΌ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΡΡ
ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΏΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π°. Π ΡΡΠΎΠΌ ΡΠ»ΡΡΠ°Π΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ CNTF Π² ΠΏΡΠΎΠ±Π΅ ΡΠΎΡΡΠ°Π²Π»ΡΠ»Π° Π² ΡΡΠ΅Π΄Π½Π΅ΠΌ 10.4Β±0.4 ΠΏΠ³/ΠΌΠ» Ρ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠΎΠΌ Π²Π°ΡΠΈΠ°ΡΠΈΠΈ 4.2% ΠΈ Π±Π»ΠΈΠ·ΠΎΡΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΡΡ
Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ ΠΊ ΠΎΠΆΠΈΠ΄Π°Π΅ΠΌΡΠΌ Π²Π΅Π»ΠΈΡΠΈΠ½Π°ΠΌ Π½Π° ΡΡΠΎΠ²Π½Π΅ 104%