277 research outputs found
Comparing Three Approaches
Hybridization-based target enrichment protocols require relatively large
starting amounts of genomic DNA, which is not always available. Here, we
tested three approaches to pre-capture library preparation starting from 10 ng
of genomic DNA: (i and ii) whole-genome amplification of DNA samples with
REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library
preparation, and (iii) library construction with a low input oriented ThruPLEX
kit (Rubicon Genomics). Exome capture with Agilent SureSelectXT2 Human AllExon
v4+UTRs capture probes, and HiSeq2000 sequencing were performed for test
libraries along with the control library prepared from 1 µg of starting DNA.
Tested protocols were characterized in terms of mapping efficiency, enrichment
ratio, coverage of the target region, and reliability of SNP genotyping.
REPLI-g- and ThruPLEX-FD-based protocols seem to be adequate solutions for
exome sequencing of low input sample
Morphological estimation of changes in cancellous raduis bone for forensic age determination
Visual researches showed that the cancellous bone of the distal radius has significant morphological changes with the increase of years. Revealed structural changes of cancellous bone allows to differentiate the age of an object and to direct a medical expert towards the proposed age group at the identification study. As a result of photocolorimetric research of involution changes of cancellous bone of the distal radius we found statistically significant increase of light transmission with the increase of years which is associated with an increase of osteoporosis. The correlation coefficient ranged from 0,63 to 0,80. On the basis of these data mathematical models that allow to determine the person's age in the forensic identification researches of the distal radius were developed (standard mean square error in determination of the age was from 6,6 to 8,2 years)
Complications of BCG vaccination: modification of the statistical procedure
There is no uniform method to conduct the epidemiological monitoring of BCG complications in the Russian Federation, Current indicators of BCG complications include the number of complication cases (cases registered within two years after vaccination) and the total number of the vaccinated children. Our aim is to discover a new indicator of BCG complications and to compare it with the indicators of the existing methods, The comparative analysis of new and existing methods of all complication cases (continuous sampling, n = 110) was conducted in the Irkutsk Region (Eastern Siberia) from 2005 to 2014, The calculating formula included the ratio of the number of children with complications vaccinated in the analyzed year to 100,000 children vaccinated in the same year, The new method shows the advantages over the existing methodological approaches, It demonstrates more adequate assessment, expressed in the highest approximation coefficient of trend model compared to the other methods, and stability of indicator over the years, The incidence of complications in the Irkutsk Region has a steady decreasing linear trend and a significant negative growth rate of the indicator (-17,9 %), The new method offers an adequate assessment and can be used as an epidemiological control system not only in the Russian Federation but in other high TB-burden countries with massive BCG vaccination of children
Современные концепции управления высшим учебным заведением
Целью и задачами статьи является исследование современных подходов к управлению вузом, их
критический анализ и возможность оптимизации процессов деятельности вуза
Regularities of structural-cellular structure of ovaries in ontogenesis
The article presents the examination of the ovaries of embryos, ovaries of human feti and ovaries of children of the tender age. The researches revealed the increase of the surface of follicles, nucleus and primary oocyte's cytoplasm that tells about the fact that further follicular maturation continues in the ovaries of a child of 2-4 months old. Also we registered the increase of nuclear cytoplasmic ratio, appearance of single mature follicles with cumulus oophorus that testifies to the fact that the ovary is functionally mature. Furthermore the research revealed increased content of lipids and DNA in follicular epithelium that also tells aboutfunctional maturity of the ovary. It was found that incretory activity of an ovary during the first year of life is provided by the cells originated from the connective tissue membrane of atresic follicles
Soft modes of collective domain-wall vibrations in epitaxial ferroelectric thin films
Mechanical restoring forces acting on ferroelastic domain walls displaced
from the equilibrium positions in epitaxial films are calculated for various
modes of their cooperative translational oscillations. For vibrations of the
domain-wall superlattice with the wave vectors corresponding to the center and
boundaries of the first Brillouin zone, the soft modes are singled out that are
distinguished by a minimum magnitude of the restoring force. It is shown that,
in polydomain ferroelectric thin films, the soft modes of wall vibrations may
create enormously large contribution to the film permittivity.Comment: 6 pages, 3 figure
The HypHI project: Hypernuclear spectroscopy with stable heavy ion beams and rare isotope beams at GSI and FAIR
The HypHI collaboration aims to perform a precise hypernuclear spectroscopy
with stable heavy ion beams and rare isotope beams at GSI and fAIR in order to
study hypernuclei at extreme isospin, especially neutron rich hypernuclei to
look insight hyperon-nucleon interactions in the neutron rich medium, and
hypernuclear magnetic moments to investigate baryon properties in the nuclei.
We are currently preparing for the first experiment with Li and C
beams at 2 AGeV to demonstrate the feasibility of a precise hypernuclear
spectroscopy by identifying H, H and
He. The first physics experiment on these hypernuclei is
planned for 2009. In the present document, an overview of the HypHI project and
the details of this first experiment will be discussed.Comment: 5 pages, 2 figures, French-Japanese symposium 2008, Paris (France
Diagnostic of the Development of Artistic Interest in Pupils to Works of Civil-Patriotic Art
Цель статьи - теоретически обосновать критерии, показатели, типы творческих заданий для диагностики развития у обучающихся художественного интереса к произведениям искусства гражданско-патриотической направленности.The purpose of the article is to theoretically substantiate the criteria, indicators, types of creative tasks for diagnosing the development of students' artistic interest in works of art of a civil-patriotic orientation
Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development
Astrocytes are resident glial cells of the central nervous system (CNS) that play complex and heterogeneous roles in brain development, homeostasis and disease. Since their vast involvement in health and disease is becoming increasingly recognized, suitable and reliable tools for studying these cells in vivo and in vitro are of utmost importance. One of the key challenges hereby is to adequately mimic their context-dependent in vivo phenotypes and functions in vitro. To better understand the spectrum of astrocytic variations in defined settings we performed a side-by-side-comparison of murine embryonic stem cell (ESC)-derived astrocytes as well as primary neonatal and adult astrocytes, revealing major differences on a functional and transcriptomic level, specifically on proliferation, migration, calcium signaling and cilium activity. Our results highlight the need to carefully consider the choice of astrocyte origin and phenotype with respect to age, isolation and culture protocols based on the respective biological question
EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in <i>Saccharomyces cerevisiae</i>
Saccharomyces cerevisiae is widely used in the biotechnology industry for production of ethanol, recombinant proteins, food ingredients and other chemicals. In order to generate highly producing and stable strains, genome integration of genes encoding metabolic pathway enzymes is the preferred option. However, integration of pathway genes in single or few copies, especially those encoding rate-controlling steps, is often not sufficient to sustain high metabolic fluxes. By exploiting the sequence diversity in the long terminal repeats (LTR) of Ty retrotransposons, we developed a new set of integrative vectors, EasyCloneMulti, that enables multiple and simultaneous integration of genes in S. cerevisiae. By creating vector backbones that combine consensus sequences that aim at targeting subsets of Ty sequences and a quickly degrading selective marker, integrations at multiple genomic loci and a range of expression levels were obtained, as assessed with the green fluorescent protein (GFP) reporter system. The EasyCloneMulti vector set was applied to balance the expression of the rate-controlling step in the β-alanine pathway for biosynthesis of 3-hydroxypropionic acid (3HP). The best 3HP producing clone, with 5.45 g.L(-1) of 3HP, produced 11 times more 3HP than the lowest producing clone, which demonstrates the capability of EasyCloneMulti vectors to impact metabolic pathway enzyme activity
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