Characteristics of long-duration inhibitory postsynaptic potentials in rat neocortical neurons in vitro

1. The characteristics of long-duration inhibitory postsynaptic potentials (l-IPSPs) which are evoked in rat frontal neocortical neurons by local electrical stimulation were investigated with intracellular recordings from anin vitro slice preparation. 2. Stimulation with suprathreshold intensities evoked l-IPSPs with typical durations of 600–900 msec at resting membrane potential. Conductance increases of 15–60% were measured at the peak amplitude of l-IPSPs (150–250 msec poststimulus). 3. The duration of the conductance increases during l-IPSPs displayed a significant voltage dependence, decreasing as the membrance potential was depolarized and increasing with hyperpolarization. 4. The reversal potential of l-IPSPs is significantly altered by reductions in the extracellular potassium concentration. Therefore it is concluded that l-IPSPs in rat neocortical neurons are generated by the activation of a potassium conductance. 5. l-IPSPs exhibit stimulation fatigue. Stimulation with a frequency of 1 Hz produces a complete fatigue of the conductance increases during l-IPSPs after approximately 20 consecutive stimuli. Recovery from this fatigue requires minutes. 6. l-IPSPs are not blocked by bicuculline but are blocked by baclofen

The impact of transglutaminase on soy protein and tofu texture

The enzyme transglutaminase was investigated for its cross-linking effect on the soy proteins of tofu. In vitro incubations confirmed that soy proteins are excellent substrates for transglutaminase, especially when denatured. The macroscopic effects resulting from the addition of transglutaminase were compared to changes at the microstructural and molecular level. Treatment produced a firmer tofu, with a significantly increased fracture force. Examination by SEM showed a change in the matrix structure, with transglutaminase resulting in a finer-stranded, uniform network that accounted for the increase in fracture force. At the molecular level, little, if any, cross-linking occurred within the tofu matrix in situ. This suggests that the change in functional properties afforded by addition of transglutaminase to tofu is due to a side reaction of the enzyme, for example hydrolysis of glutamine residues, rather than its cross-linking activity. These ideas are further explored in the accompanying paper

Possible optical detection of a fast, nearby radio pulsar PSR B1133+16

Aims: We performed deep optical observations of the field of an old, fast-moving radio pulsar PSR B1133+16 in an attempt to detect its optical counterpart and a bow shock nebula. Methods: The observations were carried out using the direct imaging mode of FORS1 at the ESO VLT/UT1 telescope in the B, R, and H_alpha bands. We also used archival images of the same field obtained with the VLT in the B band and with the Chandra/ACIS in X-rays. Results: In the B band we detected a faint (B=28.1+/-0.3) source that may be the optical counterpart of PSR B1133+16, as it is positionally consistent with the radio pulsar and with the X-ray counterpart candidate published earlier. Its upper limit in the R band implies a color index B-R <0.5, which is compatible with the index values for most pulsars identified in the optical range. The derived optical luminosity and its ratio to the X-ray luminosity of the candidate are consistent with expected values derived from a sample of pulsars detected in both spectral domains. No Balmer bow shock was detected, implying a low density of ambient matter around the pulsar. However, in the X-ray and H_alpha images we found the signature of a trail extending ~4"-5" behind the pulsar and coinciding with the direction of its proper motion. If confirmed by deeper studies, this is the first time such a trail has been seen in the optical and X-ray wavelengths. Conclusions: Further observations at later epochs are necessary to confirm the identification of the pulsar by the candidate's proper motion measurements.Comment: 11 pages, 6 figures, A&A, accepte

Granisetron vs ondansetron: is it a question of duration of 5-HT3 receptor blockade?

British Journal of Cancer (2002) 86, 1662–1663. DOI: 10.1038/sj/bjc/6600313 www.bjcancer.co

‘Even though it might take me a while, in the end, I understand it’: a longitudinal case study of interactions between a conceptual change strategy and student motivation, interest and confidence

Although there have been many investigations of the social, motivational, and emotional aspects of conceptual change, there have been few studies investigating the intersection of these factors with cognitive aspects in the regular classroom. Using a conceptual change approach, this case study reports experiences of a student of low to average prior attainment who achieved high levels of conceptual gains in five science topics over a two-year period. Her experience in the cognitive, social and affective domains was probed through analysis of interviews, student artefacts, video recordings of classroom learning, pre/post-tests and questionnaire results. For this student, peripheral or incidental persuasion of belonging to a supportive small group initially led to greater engagement with the construction of understanding through production of multiple student-generated representations, resulting in improved self-confidence and high levels of conceptual change. Evidence of transfer from performance to mastery approach goals, adoption of positive activating emotions and increased interest in science were observed. This study highlights that adoption of a multidimensional conceptual change approach with judicious organisation of small groups to support construction of verbal, pictorial and written representations of understanding may bring about changes in motivational stance, self-confidence and emotions to maximise conceptual change

19th Annual Conference on Legal Issues For Financial Institutions

Materials from the 19th Annual Conference on Legal Issues For Financial Institutions held by UK/CLE in May 1999

Computational prediction and experimental validation associating FABP-1 and pancreatic adenocarcinoma with diabetes

<p/> <p>Background</p> <p>Pancreatic cancer, composed principally of pancreatic adenocarcinoma (PaC), is the fourth leading cause of cancer death in the United States. PaC-associated diabetes may be a marker of early disease. We sought to identify molecules associated with PaC and PaC with diabetes (PaC-DM) using a novel translational bioinformatics approach. We identified fatty acid binding protein-1 (FABP-1) as one of several candidates. The primary aim of this pilot study was to experimentally validate the predicted association between FABP-1 with PaC and PaC with diabetes.</p> <p>Methods</p> <p>We searched public microarray measurements for genes that were specifically highly expressed in PaC. We then filtered for proteins with known involvement in diabetes. Validation of FABP-1 was performed via antibody immunohistochemistry on formalin-fixed paraffin embedded pancreatic tissue microarrays (FFPE TMA). FFPE TMA were constructed using148 cores of pancreatic tissue from 134 patients collected between 1995 and 2002 from patients who underwent pancreatic surgery. Primary analysis was performed on 21 normal and 60 pancreatic adenocarcinoma samples, stratified for diabetes. Clinical data on samples was obtained via retrospective chart review. Serial sections were cut per standard protocol. Antibody staining was graded by an experienced pathologist on a scale of 0-3. Bivariate and multivariate analyses were conducted to assess FABP-1 staining and clinical characteristics.</p> <p>Results</p> <p>Normal samples were significantly more likely to come from younger patients. PaC samples were significantly more likely to stain for FABP-1, when FABP-1 staining was considered a binary variable. Compared to normals, there was significantly increased staining in diabetic PaC samples (p = 0.004) and there was a trend towards increased staining in the non-diabetic PaC group (p = 0.07). In logistic regression modeling, FABP-1 staining was significantly associated with diagnosis of PaC (OR 8.6 95% CI 1.1-68, p = 0.04), though age was a confounder.</p> <p>Conclusions</p> <p>Compared to normal controls, there was a significant positive association between FABP-1 staining and PaC on FFPE-TMA, strengthened by the presence of diabetes. Further studies with closely phenotyped patient samples are required to understand the true relationship between FABP-1, PaC and PaC-associated diabetes. A translational bioinformatics approach has potential to identify novel disease associations and potential biomarkers in gastroenterology.</p

Wide-field optical imaging on ELAIS N1, ELAIS N2, First Look Survey and Lockman Hole: observations and source catalogues

We present u-, g-, r-, i- and z-band optical images and associated catalogues taken primarily with the Isaac Newton Telescope Wide Field Camera on the European Large Area ISO Survey (ELAIS) N1 and N2, First Look Survey and Lockman Hole fields comprising a total of 1000 h of integration time over 80 deg^2 and approximately 4.3 million objects. In this paper we outline the observations and data processing and characterize the completeness, reliability, photometric and astrometric accuracy of this data set. All images have been photometrically calibrated using the Sloan Digital Sky Survey and a uniform and homogeneous data set is composed over all the observed fields. Magnitude limits are u, g, r, i, z of 23.9, 24.5, 24.0, 23.3, 22.0 (AB, 5σ). These data have been used for optical identification of past and ongoing projects including the surveys ELAIS, Spitzer Wide-Area Infrared Extragalactic Survey, Spitzer Extragalactic Representative Volume Survey and Herschel Multi-tiered Extragalactic Survey