104 research outputs found
17O NMR and FT-IR study of the ionization state of peptides in aprotic solvents Application to Leu-enkephalin
AbstractThe ionization state of Leu-enkephalin in DMSO and MeCN/DMSO (4/1) solution was studied by the combined use of 17O NMR and FT-IR spectroscopy. After lyophilization or an aqueous solution at nearly neutral pH, Leu-enkephalin essentially exists in the uncharged state in MeCN/DMSO (4/1) solution. In pure DMSO, only 40% of the Leu-enkephalin molecules are in the zwitterionic state under the same conditions
Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons
Neurogliaform (RELN+) and bipolar (VIP+) GABAergic interneurons of the mammalian cerebral cortex provide critical inhibition locally within the superficial layers. While these subtypes are known to originate from the embryonic caudal ganglionic eminence (CGE), the specific genetic programs that direct their positioning, maturation, and integration into the cortical network have not been elucidated. Here, we report that in mice expression of the transcription factor Prox1 is selectively maintained in postmitotic CGE-derived cortical interneuron precursors and that loss of Prox1 impairs the integration of these cells into superficial layers. Moreover, Prox1 differentially regulates the postnatal maturation of each specific subtype originating from the CGE (RELN, Calb2/VIP, and VIP). Interestingly, Prox1 promotes the maturation of CGE-derived interneuron subtypes through intrinsic differentiation programs that operate in tandem with extrinsically driven neuronal activity-dependent pathways. Thus Prox1 represents the first identified transcription factor specifically required for the embryonic and postnatal acquisition of CGE-derived cortical interneuron properties. SIGNIFICANCE STATEMENT: Despite the recognition that 30% of GABAergic cortical interneurons originate from the caudal ganglionic eminence (CGE), to date, a specific transcriptional program that selectively regulates the development of these populations has not yet been identified. Moreover, while CGE-derived interneurons display unique patterns of tangential and radial migration and preferentially populate the superficial layers of the cortex, identification of a molecular program that controls these events is lacking.Here, we demonstrate that the homeodomain transcription factor Prox1 is expressed in postmitotic CGE-derived cortical interneuron precursors and is maintained into adulthood. We found that Prox1 function is differentially required during both embryonic and postnatal stages of development to direct the migration, differentiation, circuit integration, and maintenance programs within distinct subtypes of CGE-derived interneurons
Can group-based reassuring information alter low back pain behavior? A cluster-randomized controlled trial?
Background
Low back pain (LBP) is common in the population and multifactorial in nature, often involving negative consequences. Reassuring information to improve coping is recommended for reducing the negative consequences of LBP. Adding a simple non-threatening explanation for the pain (temporary muscular dysfunction) has been successful at altering beliefs and behavior when delivered with other intervention elements. This study investigates the isolated effect of this specific information on future occupational behavior outcomes when delivered to the workforce.
Design
A cluster-randomized controlled trial.
Methods
Publically employed workers (n=505) from 11 Danish municipality centers were randomized at center-level (cluster) to either intervention (two 1-hour group-based talks at the workplace) or control. The talks provided reassuring information together with a simple non-threatening explanation for LBP - the ‘functional-disturbance’-model. Data collections took place monthly over a 1-year period using text message tracking (SMS). Primary outcomes were self-reported days of cutting down usual activities and work participation. Secondary outcomes were self-reported back beliefs, work ability, number of healthcare visits, bothersomeness, restricted activity, use of pain medication, and sadness/depression.
Results
There was no between-group difference in the development of LBP during follow-up. Cumulative logistic regression analyses showed no between-group difference on days of cutting down activities, but increased odds for more days of work participation in the intervention group (OR=1.83 95% CI: 1.08-3.12). Furthermore, the intervention group was more likely to report: higher work ability, reduced visits to healthcare professionals, lower bothersomeness, lower levels of sadness/depression, and positive back beliefs.
Conclusion
Reassuring information involving a simple non-threatening explanation for LBP significantly increased the odds for days of work participation and higher work ability among workers who went on to experience LBP during the 12-month follow-up. Our results confirm the potential for public-health education for LBP, and add to the discussion of simple versus multidisciplinary interventions
Isotopic Grand Unification with the Inclusion of Gravity (revised version)
We introduce a dual lifting of unified gauge theories, the first
characterized by the isotopies, which are axiom- preserving maps into broader
structures with positive-definite generalized units used for the representation
of matter under the isotopies of the Poincare' symmetry, and the second
characterized by the isodualities, which are anti-isomorphic maps with
negative-definite generalized units used for the representation of antimatter
under the isodualities of the Poincare' symmetry. We then submit, apparently
for the first time, a novel grand unification with the inclusion of gravity for
matter embedded in the generalized positive-definite units of unified gauge
theories while gravity for antimatter is embedded in the isodual isounit. We
then show that the proposed grand unification provides realistic possibilities
for a resolution of the axiomatic incompatibilities between gravitation and
electroweak interactions due to curvature, antimatter and the fundamental
space-time symmetries.Comment: 20 pages, Latex, revised in various details and with added reference
Enantioselective Cyanosilylation of Ketones by a Catalytic Double-Activation Method Employing Chiral Lewis Acid and Achiral N
Energy and Exergy-Based Screening of Various Refrigerants, Hydrocarbons and Siloxanes for the Optimization of Biomass Boiler–Organic Rankine Cycle (BB–ORC) Heat and Power Cogeneration Plants
The cogeneration of power and heat was investigated for Biomass Boiler–Organic Rankine Cycle (BB–ORC) plants with the characteristics of typical units, such as the 1 MWel Turboden ORC 10 CHP. The thermodynamic analysis of the ORC unit was undertaken considering forty-two (42) dry and isentropic candidate pure working fluids. Only subcritical Rankine cycles were considered, and the pinch point temperature differences for the evaporation and condensation heat exchangers were kept constant at 10 °C in all cases. The study provides an original and unique screening of almost all pure working fluids that are considered appropriate in the literature under the same operation and optimization conditions and compiles them into a single reference. In its conclusions, the study provides useful fluid selection and design guidelines, which may be easily followed depending on the optimization objective of the ORC designer or operator. In general, hydrocarbons are found to lie in the optimum middle range of the fluid spectrum, between the siloxanes that maximize the production of mechanical power and the refrigerants that maximize the production of heat. Specific hydrocarbon fluids, such as cyclopentane, heptane, hexane, benzene, and toluene, are found as rational options for maximum mechanical efficiency when operating with practically feasible condensation pressures between 10 and 200 kPa. At condensation pressures below 10 kPa, ethylbenzene, o-xylene, m-xylene, p-xylene, and nonane are also found to be feasible options. Finally, cyclopentane, hexane, and MM (hexamethyldisiloxane) are selected as the most appropriate options for cogeneration plants aiming simultaneously at high mechanical power and maximum temperature water production. © 2022 by the authors
IR-Methode zur schnellen Identifizierung von Dealkylierungsprodukten in den Addukten von Metallsalzen mit neutralen Organophosphorylestern
In-Depth Characterization of Layer 5 Output Neurons of the Primary Somatosensory Cortex Innervating the Mouse Dorsal Spinal Cord
Neuronal circuits of the spinal dorsal horn integrate sensory information from the periphery with inhibitory and facilitating input from higher central nervous system areas. Most previous work focused on projections descending from the hindbrain. Less is known about inputs descending from the cerebral cortex. Here, we identified cholecystokinin (CCK) positive layer 5 pyramidal neurons of the primary somatosensory cortex (CCK + S1-corticospinal tract [CST] neurons) as a major source of input to the spinal dorsal horn. We combined intersectional genetics and virus-mediated gene transfer to characterize CCK+ S1-CST neurons and to define their presynaptic input and postsynaptic target neurons. We found that S1-CST neurons constitute a heterogeneous population that can be subdivided into distinct molecular subgroups. Rabies-based retrograde tracing revealed monosynaptic input from layer 2/3 pyramidal neurons, from parvalbumin positive cortical interneurons, and from thalamic relay neurons in the ventral posterolateral nucleus. Wheat germ agglutinin-based anterograde tracing identified postsynaptic target neurons in dorsal horn laminae III and IV. About 60% of these neurons were inhibitory and about 60% of all spinal target neurons expressed the transcription factor c-Maf. The heterogeneous nature of both S1-CST neurons and their spinal targets suggest complex roles in the fine-tuning of sensory processing
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