Die rol van die priester in die ‘troonopvolgingsgeskiedenis’

The so-called 'Succession Narrative' or ’Court History' (2 Sam. 9-10 and 1 Kings 1-2) bears very little testimony to the role of the priests. This article represents an attempt to examine the position of little priestly community, the priestly office-bearers and their office, the pail played by the priests in the crowning of the king and the fulfillment of their office to determine the role of the priests in the history of revelation ('theology') as seen in the Succession Narrative. It will become clear that the priestly order was indeed not very prominent and that there were many shortcomings. This however, was part of the fulfillment of God’s covenant promises that there would be a successor to David. Wiile God did not specifically use the priests in the succession to David’s throne, the priests were not ignored in the revelation since their presence and their officiating emphasized the presence of the Lord

Die beklemtoning van die geslagte of geslagslyste in Genesis

Julius Wellhausen het reeds die belangrikheid van die geslagte of geslagslyste (toledot) besef en het in sy onderskeiding van die verskillende bronne tole­ dot as een van die karakteristieke woorde van die P-dokument beskou. P het dit later toegevoeg om die inhoud van die uit bronne saamgestelde Pen- tateug en in die besonder Genesis skematies te konstrueer. Die toledot-ioT- mule kom dan normaalweg aan die begin van sekere P-stukke voor. Die uit- sondering is Gen. 1:4a, waar dit die slotsin van ’n P-stuk vorm. As motive- ring vir hierdie afwyking is later verskeie oplossings aan die hand gedoen, bv. aat so ’n formule nie by die forse inleiding soos in Gen.l: 1 gevind tuishoort me. Die verbinding van toledot met die P-stukke is van verskillende kante bevraagteken (o.a. Eerdmans, Lohr, Volz, Smend, Eichrodt ens. — cf. Hul­ ling, 1964:216-26). Nadat Kulling (ibid., p. 217) ’n deeglike studie in dié verband ondemeem het, het hy afdoende bewys dat die to/edoi-formules nie aan die P-stukke verbind mag word nie, en dit was daarom ook nie die mer- kers wat ’n latere hand gebruik het om die boek Genesis te konstrueer nie. Hiermee het die belangrikheid van die toledot-formules nie afgeneem nie

Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining

This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Max F. Perutz Laboratories, Campus Vienna Biocenter, Vienna, Austria and Ernst Thuer, Centre for Genomic Regulation, Barcelona, Spain, for advice on statistical approaches. Neil Gow acknowledges the support of the Wellcome Trust and the MRC Centre for Medical MycologyPeer reviewedPublisher PD

Trace Metals Bioaccumulation Potentials of Three Indigenous Grasses Grown on Polluted Soils Collected Around Mining Areas in Pretoria, South Africa

The rapid increase in the number of industries may have increased the levels of trace metals in the soil. Phytoremediation of these polluted soils using indigenous grasses is now considered an alternative method in remediating these polluted soils. The present study investigated and compared the ability of three indigenous grasses as bioaccumulators of trace metals from polluted soils. Seeds of these grasses were introduced into pots containing polluted soil samples after the addition of organic manure. The seeds of the grasses were allowed to germinate and grow to maturity before harvesting. The harvested grasses were later separated into shoots and roots and the trace metal contents were determined using ICP –MS. From all the grasses, the concentrations of trace metals in the roots were more than those recorded in the shoot with a significant difference (P < 0.05). The transfer factor (TF) showed that Zn was the most bioaccumulated trace metals by all the grasses followed by Pb, Mn, and Cu respectively. Chromium concentration from the shoot of the grasses was in the order Urochlora moasambicensis > Themeda trianda > Cynodon dactylon. The study concluded that the three grasses used were all able to bioaccumulate trace metals in a similar proportion from the polluted soils. However, since livestock feed on these grasses, they should not be allowed to feed on the grasses used in this study especially when harvested from a polluted soil due to their bioaccumulative potentials

Redox Proteomics and Platelet Activation: Understanding the Redox Proteome to Improve Platelet Quality for Transfusion.

Blood banks use pathogen inactivation (PI) technologies to increase the safety of platelet concentrates (PCs). The characteristics of PI-treated PCs slightly differ from those of untreated PCs, but the underlying reasons are not well understood. One possible cause is the generation of oxidative stress during the PI process. This is of great interest since reactive oxygen species (ROS) act as second messengers in platelet functions. Furthermore, there are links between protein oxidation and phosphorylation, another mechanism that is critical for cell regulation. Current research efforts focus on understanding the underlying mechanisms and identifying new target proteins. Proteomics technologies represent powerful tools for investigating signaling pathways involving ROS and post-translational modifications such as phosphorylation, while quantitative techniques enable the comparison of the platelet resting state versus the stimulated state. In particular, redox cysteine is a key player in platelet activation upon stimulation by different agonists. This review highlights the experiments that have provided insights into the roles of ROS in platelet function and the implications for platelet transfusion, and potentially in diseases such as inflammation and platelet hyperactivity. The review also describes the implication of redox mechanism in platelet storage considerations

Non-classical Photon Statistics For Two-mode Optical Fields

The non-classical property of subpoissonian photon statistics is extended from one to two-mode electromagnetic fields, incorporating the physically motivated property of invariance under passive unitary transformations. Applications to squeezed coherent states, squeezed thermal states, and superposition of coherent states are given. Dependences of extent of non-classical behaviour on the independent squeezing parameters are graphically displayed.Comment: 15 pages, RevTex, 5 figures, available by sending email to [email protected]

Die begronding van geregtigheid - 'n Perspektief op die wettebundels van die Pentateug

We can ascribe a certain sense of righteousness to all people. Using the legal codes of the Pentateuch (the Book of the Covenant, Deuteronomy and Leviticus-Numhers), this paper undertakes to answer the question of the extent of the code's foundation in God and His relationship with people

The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates.

Since 1990, several techniques have been developed to photochemically inactivate pathogens in platelet concentrates, potentially leading to safer transfusion therapy. The three most common methods are amotosalen/UVA (INTERCEPT Blood System), riboflavin/UVA-UVB (MIRASOL PRT), and UVC (Theraflex-UV). We review the biology of pathogen inactivation methods, present their efficacy in reducing pathogens, discuss their impact on the functional aspects of treated platelets, and review clinical studies showing the clinical efficiency of the pathogen inactivation methods and their possible toxicity

Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes

Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. The latter are then reduced to copper metal and further detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere

Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy

Scanning Electrochemical Microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained using a standard protocol involving copper salts. Salts are then reduced to copper and detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere. This method enables unspecific protein adsorption mapping on polymeric membranes and into microchannels without any requirement of enzymatic activity or affinity to a labelled secondary reporter